Catalog: OM295324 Scan to get more validated information
YWHAB Antibody
Catalog: OM295324 100ug
Product profile
Product name YWHAB Antibody
Antibody Type Primary Antibodies
Product description YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by bindi ng to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, sugge sting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript varia nts differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variant s encode the same protein, however, they are differentially expressed in hematopoietic cells.1) Komori, T. , et al., Acta Neuropathol. 106(1):66-70 (2003). Cavet, M.E., et al., J. Biol. Chem. 278(20):18376-18383 (200 3). Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). Shumway, S.D., et al., J. Biol. Chem. 278(4):2089 -2092 (2003). Parvaresch, S., et al., FEBS Lett. 532(3):357-362 (2002).
Immunogen This YWHAB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide bet ween 217-246 amino acids from the C-terminal region of human YWHAB.
Key Feature
Clonality Polyclonal
Isotype Ig
Host Species Rabbit
Tested Applications IF ,IHC-P ,WB
Species Reactivity Human
Concentration 1 mg/ml
Target Information
Gene Symbol YWHAB
Alternative Names 14-3-3 protein beta/alpha, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1, 14-3-3 protein beta/ alpha, N-terminally processed, YWHAB
Molecular Weight(MW) 28 kDa Molecular Weight(MW) 28 kDa
Sequence Similarities Predicted species reactivity based on immunogen sequence: Bovine
Database Links
Entrez Gene 7529
Protein Accession P31946
Application Application Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application Antibody is used in Western blot to detect YWHAB in Jurkat cell lysate.
Application Western blot analysis of YWHAB using rabbit polyclonal YWHAB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAB gene.
Application Confocal immunofluorescent analysis of YWHAB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application Notes For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 For IF starting dilution is : 1:10~50:
Additional Information
Form Liquid
Storage Instructions Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be t aken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high tempera
tures.
Storage Buffer Supplied in PBS with 0.09% (W/V) sodium azide.
Note The product is for research use only,not for use in diagnostic or therapeutic procedures. OmnimAbs.com [email protected] 506 N. GARFIELD AVE # 210 ALHAMBRA, CA 91801 This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt