6Th Rosaceous Genomics Conference (RGC6)
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6th Rosaceous Genomics Conference (RGC6) Mezzocorona (Trento), Italy, 30th September - 4th October 2012 FONDAZIONE EDMUNDFONDAZIONE MACH EDMUND MACH eous Genomics osac Con Rosaceous Gen nce th R fere Sixth omics Confere Six nc ISTITUTO AGRARIOISTITUTOe AGRARIO Under the Auspices ofUnder the the Auspices of the DI SAN MICHELERG ALL’ADIGECDI SAN6 MICHELE ALL’ADIGERGAutonomousC 6 ProvinceAutonomous of Trento Province of Trento 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 th 6 Rosaceous Genomics Conference (RGC6) th th Mezzocorona (Trento), Italy, 30 September‐4 October 2012 ROGRAM AND OOK OF BSTRACTS P B A Under the Auspices of the Autonomous Province of Trento 3 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 Event organized by Event organized by Provincia Autonoma di Trento Provincia Autonoma di Trento and supported by and supported by Cover pictures: Flowering apples and Brenta Dolomites (Courtesy of the Tourism Board of Val di Non - Trentino - Italy and Centre for Technological Transfer Fondazione Edmund Mach) 4 4 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 th 6 Rosaceous Genomics Conference (RGC6) Program Sunday, 30th September Arrival and Accommodation 17:00 Registration at PalOpening lecture: J.arotari, Mezzocorona Salse 18:20 ‐ Plant and animal genome structural, functional and evolutionary plasticity 19:00‐21:30 Welcome dinner in the Historical Cellar Monday, 1st October 8:00‐09:00 Registration R. Viola 8:30 Plenary ‐ Welcome address lecture: S. Myles 9:00 ‐ Genotyping‐by‐sequencing in apple: Enormous promise and significant challenges Session 1 – Structural Genomics Chair: Sue Gardiner S. Gardiner Pyrus communis 9:30 ‐ A draft genome sequence of European pear ( L. ‘Bartlett’) 5 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 T.M. Davis 10:10 S. Montanari‐ Genome compositions in the oct Pyrus oploid and decaploid strawberries Pyrus 10:25 ‐ Identification of single nucleotide polymorphisms (SNPs) and evaluation for genetic mapping in European pear and inter‐specific hybrids J.M. Bradeen 10:25 ‐ The RosaR80 system: a framework for cross‐species comparative analyses of R‐genes from Rosaceous species 10:55 Coffee break AFFYMETRIX SPECIAL EVENT. F. Brew 11:15 ‐ How do you accelerate your genomics program from NGS to molecular breeding? Session 2 Functional Genomics Chair: Herman Silva H. Silva Prunus avium 11:45 ‐ A functional genomics approach to understand cracking susceptibility in sweet cherry (G. Sanchez ) 12:15 ‐ An integrative “omics” approach identifies new candidate genes to impact aroma volatiles in peach fruit T. Hytönen Fragaria vesca 12:30 ‐ Interconnected molecular pathways control vegetative and floral development in R. Schaffer 12:45 ‐ The complexity of apple fruit ripening 13:00 Lunch Session 3 Emerging Omics Chair: Fulvio Mattivi R. Wehrens 14:00 V. Shulaev ‐ High‐throughput metabolomics ‐ challenges for bioinformatics 14:30 ‐ Harvesting the strawberry genome: from genes to metabolic networks 6 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 A. Orellana Prunus persica 14:45 ‐ Unravelling the proteome of the mesocarp during softening 15:0017:00 First poster session (Even numbers) 17:0019:00 Second poster session (Odd numbers) 15:0017:00 SPECIAL EVENT Illumina Genome Studio workshop Session 1 17:0019:00 SPECIAL EVENT. Illumina Genome Studio workshop Session 2 . 15:0019:00 SPECIAL EVENT Global Crop Diversity Trust Expert consultation workshop on the use of crop wild relatives for . pre‐breeding in apple 15:0019:00 SPECIAL EVENT Meeting of the COST Action on cherries . Tuesday, 2nd October Plenary lecture: J. Giovannoni 8:45 ‐ Tomato as a model for analysis of molecular control of fruit ripening Session 4 Plant Genetics Chair: Amy Iezzoni A. Iezzoni Prunus 9:25 ‐ The genetic control of fruit size in cherry ( ): from phenotype to candidate genes S. Kumar 9:55 ‐ Genome‐wide association study of apple fruit traits in an experimental population A. Monfort 10:10 ‐ A walk around the phenotype of diploid strawberry using a NILs collection 7 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 C. Romero 10:25 ‐ Self‐compatibility associated with pollen modifier gene(s) in apricot 10:40 Coffee break ILLUMINA SPECIAL EVENT R. Scavelli 11:00‐11:30 . ‐ Genotyping by sequencing with Illumina technology Session 5 – Fruit quality Chair: Elizabeth Dirlewanger E. Dirlewanger QueroGarcia 11:30 and J. ‐ The genetic control of fruit quality traits in two prunus species: peach and cherry F. Costa 12:00 – Genomic approaches towards efficient assisted breeding for fruit quality in apple R. Pirona 12:15 E.J. Buck ‐ Genetic dissection of peach fruit quality traits 12:30 ‐ Genetic control of fruit sugar content across the Rosaceae 12:45 Lunch Session 6 Applied Molecular Breeding Chair: Pere Arús P. Arús 13:45 ‐ Towards the enrichment of the peach gene pool with alleles from wild species: the collection of peach‐almond introgression lines S. Martens Malus domestica Pyrus communis 14:15 ‐ Genetic and molecular characterisation of apple‐pear hybrids ( M. Davey × ) 14:30 K. Gasic ‐ Allelic‐specific markers for fruit vitamin C breeding in apple Xanthomonas arboricola pv. pruni 14:45 ‐ Mapping QTLs Associated with Resistance to Bacterial Spot ( ) in Peach 15:00 Coffee break 8 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 Session 7 International ‘Omics’ Projects Chair: Francois Laurens F. Laurens 15:20 M. Jänsch ‐ Progress in EU‐FruitBreedomics Rvi2 Rvi4 Rvi11 15:35 ‐ Precise mapping an identification of SNPs associated with the apple scab resistance genes D. Micheletti , and 15:50 ‐ Genetic variability description in a wide germplasm of domesticated peach through high throughput genotyping M. Bink 16:05 E.van de ‐ Discovery & interpretatio Weg n of multiple linked QTL 16:20 ‐ Towards a large sized Axiom SNP array for the allo‐octoploid strawberry C. Peace 16:35 ‐ Jewels in the apple genome: RosBREED’s conversion of reported trait loci for fruit quality into DNA tests routinely used in breeding 19:30‐23:30 Social Dinner at Castello di Toblino – Poster awards Wednesday, 3rd October Plenary lecture: L. Han 8:30 ‐ Strategy for identification of functionally important genes and marker‐assisted plant breeding using NGS technology Session 8 Genetic Engineering Chair: Henk Schouten H. Schouten 9:40 C. Gessler ‐ New plant breeding techniques in the EU 10:00 H. Flachowsky ‐ Can genetic engineering reduce pesticide use in apple orchards? 10:20 ‐ Trans‐ and cis‐genic approaches to improve apple breeding at JKI in Dresden‐Pillnitz A. Chambers Fragaria 10:35 ‐ Functional Genomics 9 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 10:50 Coffee break Session 9 Computational Biology and Bioinformatics Chair: Dorrie Main S. Jung 11:10 J. Ward ‐ Ten years of GDR: Current resources and functionality GDR Rubus idaeus 11:40 – Progress on the development of a genome sequence for red raspberry J. Jansen 11:55 ‐ Statistical tools and software for the construction of integrated genetic linkage maps of outbreeding species using SNP markers with applJ.M. Celton e as a model 12:10 ‐ Re‐annotation and transcript expression analysis of the apple genome 12:25 Lunch Session 10 Pests and Diseases Chair: Bert Abbott B. Abbott 13:40 ‐ Exploiting the peach genome sequence for identification of candidate genes for disease resistanceH. Duval in fruit and forest trees 13:55 ‐ Marker assisted breeding for peach seedling rootstocks resistant to root‐knot‐nematodes V. Decroocq Prunus armeniaca 14:10 ‐ Integrated approaches in for resistance to sharka disease A. Peil Malus × robusta Erwinia Amylovora 14:25 ‐ Indications for a gene‐for‐gene relationship in the – host pathogen system 14:40‐15:40 Discussion Conclusion and farewell 15:40‐17:40 RosExec and RosIGI meetings 10 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 Thursday, 4th October 8:00‐20:00 Dolomites bus trip. For those who want to experience one of the best mountain thrills of Europe, we have planned a trip on the Dolomites and surrounding areas. In case of bad weather we have an alternative programme that includes a visit of Bolzano historical centre and of the Ötzi‐The Iceman Museum, with lunch in one of the renewed brewery of the town. 11 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 Abstracts Keynote speakers 13 6th Rosaceous Genomics Conference Mezzocorona, Italy – 30th September ‐04th October 2012 Tomato as a model for analysis of molecular control of fruit ripening Jim Giovannoni(1) (1) Boyce Thompson Institute for Plant Research and USDA‐ARS, Tower Road, Cornell University campus, Ithaca, NY 14853 USA Tomato is one of the most studied species for fleshy fruit development and climacteric ripening. Availability of a high quality tomato genome sequence has facilitated genetic inquiries via approaches that were not possible until now. Advances in understanding the genetic regulation of tomato fruit ripening to date have focused mostly on characterization of genes and gene expression patterns associated with ripening. Identification of specific genes underlying ethylene biosynthesis and downstream