First Records of Three Genera of Metopiinae (Hymenoptera

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55 HOSTED BY Contents lists available at ScienceDirect 56 57 Journal of Asia-Pacific Biodiversity 58 59 60 journal homepage: http://www.elsevier.com/locate/japb 61 62 63 Original article 64 65 1 First records of three genera of Metopiinae (Hymenoptera, 66 2 67 3 Ichneumonidae) from South Korea with description of one new 68 4 species 69 5 70 6 1 2 1,* 71 7 Q8 Jin-Kyung Choi , Kyong-In Suh , Jong-Wook Lee 72 8 1 Department of Life Sciences, Yeungnam University, Gyeongsan, South Korea 73 9 2 Korean Entomological Institute, Korea University, Seoul 136-701, South Korea 74 10 75 11 76 12 article info abstract 77 13 78 14 Article history: Trieces koreanus Choi & Lee, sp. nov. is described and illustrated in the South Korean fauna. The genera 79 Received 13 April 2016 fi 15 Periope, Stethoncus, and Trieces are recorded for the rst time from South Korea. Description with pho- 80 16 Received in revised form tographs of the new species and diagnoses for each species are provided. 27 April 2016 81 Copyright Ó 2016, National Science Museum of Korea (NSMK) and Korea National Arboretum (KNA). 17 Accepted 7 May 2016 82 Production and hosting by Elsevier. This is an open access article under the CC BY-NC-ND license (http:// 18 Available online xxx 83 creativecommons.org/licenses/by-nc-nd/4.0/). 19 84 20 Keywords: 85 Korea 21 86 22 Periope Stethoncus 87 23 taxonomy 88 24 Trieces 89 25 90 26 91 27 92 28 93 29 Introduction science). In this study, we report a new species and four newly 94 30 recorded species from South Korea. Q1 95 31 Metopiinae Förster, 1869 is a moderately large ichneumonid 96 32 group comprising more than 830 described species worldwide. 97 Materials and methods 33 According to Baltazar (1964), the group consists of koinobiont 98 34 endoparasitoids in pyraloids and tortricoids of lepidopterous 99 Materials used in this study were collected by sweeping and 35 larvae, which emerge from the pupae of their hosts. The genus 100 Malaise trapping, after which they were deposited in the animal 36 Trieces Townes, 1946 comprises 69 species (Tolkanitz 2010; Yu et al 101 systematic laboratory of Yeungnam University (YNU, Gyeongsan, 37 2012; Zhang et al 2016), including the newly described species, 102 South Korea). Morphological terminology follows mostly that of 38 Trieces etuokensis Sheng, 2016, from China by Zhang et al (2016). 103 Gauld et al (2002). Specimens were examined using an AxioCam 39 Also, the genera Periope Haliday, 1838 and Stethoncus Townes & 104 MRc5 camera attached to a stereomicroscope (Zeiss SteREO Dis- 40 Townes, 1959 comprise only five species worldwide (Yu et al 105 covery. V20; Carl Zeiss, Göttingen, Germany), processed using 41 2012). The genera Periope, Stethoncus, and Trieces have not been 106 AxioVision SE64 software (Carl Zeiss), and optimized using a Delta 42 studied yet in South Korea. Three genera of South Korean Meto- 107 imaging system (i-solution; IMT i-Solution Inc., Vancouver, Can- 43 piinae are discussed here: Periope with one species, Stethoncus with 108 ada). Distributional data mainly follow that of Yu et al (2012). 44 one species, and Trieces with three species (one of them new to 109 Species description and diagnoses are based on South Korean 45 110 specimens. 46 111 Abbreviations are as follows: GN, Gyeongsangnam-do; GG, 47 112 Gyeonggi-do. AEI, American Entomological Institute, Gainesville, 48 113 FL, USA (H. Townes collection); HU, Hokkaido University, Faculty of 49 * Corresponding author. Tel.: þ82 53 810 2376. 114 Agriculture, Entomological Institute, Sapporo, Japan; MZ, Musée 50 E-mail address: [email protected] (J.-W. Lee). 115 Zoologique, Place Riponne, Switzerland; TMA, Termeszettudoma- 51 Peer review under responsibility of National Science Museum of Korea (NSMK) and 116 Korea National Arboretum (KNA). nyi Muzeum Allattara, Budapest, Hungary. 52 117 53 http://dx.doi.org/10.1016/j.japb.2016.05.001 118 54 pISSN2287-884X eISSN2287-9544/Copyright Ó 2016, National Science Museum of Korea (NSMK) and Korea National Arboretum (KNA). Production and hosting by Elsevier. 119 This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Please cite this article in press as: Choi J-K, et al., First records of three genera of Metopiinae (Hymenoptera, Ichneumonidae) from South Korea with description of one new species, Journal of Asia-Paciﬁc Biodiversity (2016), http://dx.doi.org/10.1016/j.japb.2016.05.001 JAPB157_proof ■ 27 May 2016 ■ 2/7

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1 Q2 Systematic accounts Diagnosis 66 2 67 3 Family Ichneumonidae Latreille, 1802 Color. Black. Labrum, palpi, projection of upper face, fore and 68 4 Subfamily Metopiinae Förster, 1869 mid legs except basal coxae, spots on dorsal femora of fore and mid 69 5 Genus Periope Haliday, 1838 legs, hind trochanter and tibia except apically, hind tarsi and tegula 70 6 Periope Haliday, 1838:112e121. Type species: Periope auscultator yellow (Figures 1A and 1B). Antenna except scape and first flag- 71 7 Haliday. ellomere brown. 72 8 Monoplectron Holmgren, 1856:1e104. Type species: Monoplec- Face rugosely punctate. Malar space granulate, shorter than 73 9 tron zygaenator Holmgren. basal width of mandible. Projection of upper face recurved. Vertex 74 10 Oligoplectron Förster, 1869:135e221. Type species: Periope densely punctate. Antenna with at least 21 flagellomeres. Pronotum 75 11 auscultator Haliday. New name for primary homonym. polished, with hairs. Scutellum convex. Epicnemial carina incom- 76 12 Monoplectrochus Heinrich, 1949:101e127. Type species: Mono- plete; mesopleuron densely punctate; speculum shiny (Figure 1C). Q3 77 13 plectrochus hoerhammeri Heinrich Metapleuron slightly punctate. Fore wing with stalked areolet 78 14 (Figure 1E). Hind wing with nine distal hamuli. Tergites coarsely 79 15 Periope hoerhammeri (Heinrich, 1949) punctate; first tergite with lateral carinae at anterior half of first 80 16 Monoplectrochus hoerhammeri Heinrich, 1949:101e127. Type: tergite in lateral view. 81 17 male; Type depository: AEI. Material examined. South Korea: [GG] 1_, Bokwangsa, 1 v 1984 82 18 (JW Lee). 83 19 84 20 85 21 86 22 87 23 88 24 89 25 90 26 91 27 92 28 93 29 94 30 95 31 96 32 97 33 98 34 99 35 100 36 101 37 102 38 103 39 104 40 105 41 106 42 107 43 108 44 109 45 110 46 111 47 112 48 113 49 114 50 115 51 116 52 117 53 118 54 119 55 120 56 121 57 122 58 123 59 124 60 125 61 126 62 127 63 128 64 Figure 1. Periope hoerhammeri. A, Head, frontal view. B, Habitus, lateral view. C, Head and mesosoma, lateral view. D, Propodeum, dorsal view. E, Fore wing. Scale bars: 0.2 mm (A, 129 65 D); 0.5 mm (C, E); 1 mm (B). 130

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1 Distribution. South Korea (new record), Japan, Germany, Face coarsely and sparsely punctate; projection of upper face 90 66 2 Lithuania, Poland, Russia (Primor’ye Kray), Ukraine. recurved. Frons convex, sparsely punctate (Figure 2B). Malar space 67 3 longer than basal width of mandible. Occipital carina indistinct; 68 Genus Stethoncus Townes & Townes, 1959 4 occiput sunken. Pronotum sunken and glabrous except upper area 69 Stethoncus Townes & Townes, 1959: 167. Type species: Stetho- 5 (Figure 2C). Mesopleuron swollen, shiny, and sparsely punctate. 70 ncus arcticus Townes & Townes. 6 Mesoscutum sparsely punctate, with notauli (Figure 2D). Scutellum 71 7 Stethoncus sulcator Aubert, 1963 elongate and polished, without lateral carina. Propodeum well- 72 8 Stethoncus sulcator Aubert, 1963:82e86. Type: male; Type de- developed carinae (Figure 2E); propodeal spiracle round, reach 73 9 pository: MZ. pleural carina. Metapleuron glabrous. Fore wing without areolet. 74 10 Hind wing with ﬁve distal hamuli. Legs stout. Tergites shiny and 75 11 Diagnosis impunctate; ﬁrst tergite with median longitudinal carinae. 76 12 Material examined. South Korea: [GN] 1_, Sancheong-gun, 77 13 Color. Black. Palpi, mandible, antenna, tegula and legs yellowish Sicheon-myeon, Naedae-ri Jirisan National Park, Saeseoksanjang, 78 14 brown; hind coxa darkened (Figures 2A and 2B). 12 2001 (JW Lee). 79 15 80 16 81 17 82 18 83 19 84 20 85 21 86 22 87 23 88 24 89 25 90 26 91 27 92 28 93 29 94 30 95 31 96 32 97 33 98 34 99 35 100 36 101 37 102 38 103 39 104 40 105 41 106 42 107 43 108 44 109 45 110 46 111 47 112 48 113 49 114 50 115 51 116 52 117 53 118 54 119 55 120 56 121 57 122 58 123 59 124 60 125 61 126 62 127 63 128 64 129 Figure 2. Stethoncus sulcator. A, Habitus, lateral view. B, Head, frontal view. C, Head and mesosoma, lateral view. D, Head and mesoscutum, dorsal view. E, Scutellum and pro- 65 podeum, dorsal view. Scale bars: 0.2 mm (B, C, D, E); 1 mm (A). 130

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1 Distribution. South Korea (new record), Austria, Bulgaria, France, Face black with yellow projection (Figure 4B). Antenna with 33 66 2 Germany, Japan, Poland, Russia (Buryatskaya Respublika, Krasnodar flagellomeres. T. koreanus Choi & Lee sp. nov. 67 3 Kray), Switzerland, Ukraine. 68 4 Trieces bellulus Kusigemati, 1984 69 Genus Trieces Townes, 1946 5 Trieces bellulus Kusigemati, 1984: 245e263. Type: female; Type 70 Trieces Townes, 1946: 60. Type species: Exochus texanus Cresson. 6 depository: TMA. 71 7 72 8 73 Key to species of South Korean Trieces Diagnosis 9 74 10 75 1. Metapleuron with vertical slit-shaped depression posteriorly. Color. Black. Face, clypeus, palpi, ventral part of antenna, tegula, 11 76 Occipital carina present. 2 fore and mid legs, hind leg except coxa and femur, and ovipositor 12 77 - Metapleuron without vertical slit-shaped depression posteri- yellowish brown; hind coxa dark reddish brown; hind femur brown 13 78 orly. Occipital carina absent. T. bellulus Kusigemati (Figures 5A and 5B). 14 79 2. Head not widened posteriorly behind eyes in dorsal view. Face sparsely punctate. Antenna with 21 flagellomeres. 15 80 Temple not convex, shorter than transverse eye diameter in Mandible granulate. Malar space shorter than basal width of 16 81 lateral view. Face yellowish brown (Figure 3B). Antenna with mandible. Frons shiny without median longitudinal ridge. Occipital 17 Q4 82 less than 24 flagellomeres. T. homonae Kusigemati carina absent. Pronotum glabrous, upper corner of pronotum 18 83 - Head widened posteriorly behind eyes in dorsal view. Temple sparsely punctate (Figure 5C). Mesopleuron sparsely punctate 19 84 convex, longer than transverse eye diameter in lateral view. anteriorly; speculum area glabrous and flat, without pit (Figure 5C). 20 85 21 86 22 87 23 88 24 89 25 90 26 91 27 92 28 93 29 94 30 95 31 96 32 97 33 98 34 99 35 100 36 101 37 102 38 103 39 104 40 105 41 106 42 107 43 108 44 109 45 110 46 111 47 112 48 113 49 114 50 115 51 116 52 117 53 118 54 119 55 120 56 121 57 122 58 123 59 124 60 125 61 126 62 127 63 128 64 Figure 3. Trieces homonae. A, Habitus, lateral view. B, Head, frontal view. C, Head and mesosoma, lateral view. D, Head and mesoscutum, dorsal view. E, Propodeum and tergites, 129 65 dorsal view. F, Wings. Scale bars: 0.1 mm (B, D); 0.2 mm (C, E); 0.5 mm (A). 130

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1 66 2 67 3 68 4 69 5 70 6 71 7 72 8 73 9 74 10 75 11 76 12 77 13 78 14 79 15 80 16 81 17 82 18 83 19 84 20 85 21 86 22 87 23 88 24 89 25 90 26 91 27 92 28 93 29 94 30 95 31 96 32 97 33 98 34 99 35 100 36 101 37 102 38 103 39 104 40 105 41 106 42 107 43 108 44 109 45 Figure 4. Trieces koreanus Choi & Lee sp. nov. A, Habitus, lateral view. B, Head, frontal view. C, Head and mesosoma, lateral view. D, Head and mesoscutum, dorsal view. E, Second 110 46 and third tergites, dorsolateral view. Scale bars: 0.2 mm (B, D, E); 0.5 mm (C); 1 mm (A). 111 47 Mesoscutum convex, without notaulus (Figure 5D). Metapleuron Diagnosis 112 48 shiny; lower and posterior area of metapleuron with transverse 113 49 wrinkles; without vertical slit-shaped depression posteriorly. Color. Black. Face, scape, palpi, fore and mid legs and tegula 114 50 Scutellum elongate, with lateral carinae. Propodeum rugosely yellowish brown to brown (Figures 3B and 3C). Antenna brown. 115 51 punctate. Fore wing without areolet (Figure 5F). Hind wing with six Hind leg brown to dark brown; hind coxa darkened basally 116 52 distal hamuli. Legs stout. First to third tergites rugosely punctate; (Figure 3A). 117 53 first to basal half of third tergites with lateral carinae in lateral view. Face sparsely punctate. Head not widened posteriorly behind 118 54 Second to half of third tergites with median longitudinal carinae eyes in dorsal view. Antenna with 24 flagellomeres; pedicel as long 119 55 distinctly in dorsal view (Figure 5E). Fourth to seventh tergites as scape. Mandible glabrous except basal area granulate. Malar 120 56 finely punctate. space shorter than basal width of mandible. Frons shiny without 121 57 Material examined. South Korea: 1\, Daegu-si, Dalseo-gu, median longitudinal ridge. Temple not convex, shorter than 122 58 Daegok-dong, Daegu Arboretum, alt. 88 m, 22e23 viii 2012 (SG transverse eye diameter in lateral view. Occipital carina complete. 123 59 Kang). Pronotum glabrous (Figure 3C), upper corner of pronotum closely 124 60 Distribution. South Korea (new record), Mongolia, Russia (Chita punctate. Mesopleuron densely punctate anteriorly; speculum area 125 61 Oblast, Kamchatka Oblast, Primor’ye Kray, Tuvinskaya Respublika). flat, without pit. Mesoscutum convex, without notaulus. Meta- 126 62 pleuron shiny, with hairs on upper area; lower area of metapleuron 127 Trieces homonae Kusigemati, 1967 63 with transverse wrinkles; with vertical slit-shaped depression 128 Trieces homonae Kusigemati, 1967:45e49. Type: female; Type 64 posteriorly. Scutellum elongate, with lateral carinae. Propodeum 129 65 depository: HU. 130

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1 66 2 67 3 68 4 69 5 70 6 71 7 72 8 73 9 74 10 75 11 76 12 77 13 78 14 79 15 80 16 81 17 82 18 83 19 84 20 85 21 86 22 87 23 88 24 89 25 90 26 91 27 92 28 93 29 94 30 95 31 96 32 97 33 98 34 99 35 100 36 101 Figure 5. Trieces bellulus. A, Habitus, lateral view. B, Head, frontal view. C, Mesosoma, lateral view. D, Head and mesoscutum, dorsal view. E, Propodeum and basal tergites, dorsal 37 102 view. F, Fore wing. Scale bars: 0.2 mm (B, C, D, E); 0.5 mm (A, F). 38 103 39 104 40 105 41 106 with parallel median longitudinal carinae, without costula truncate. Mandible small, weakly narrowed toward apex; upper 42 107 (Figure 3D). Fore wing without areolet (Figure 3A). Hind wing with tooth distinctly longer than lower tooth. Malar space approxi- 43 108 six distal hamuli. Legs stout. Metasoma finely and densely punctate. mately as long as basal width of mandible. Temple convex and 44 109 First tergite with median longitudinal carinae distinctly in dorsal shiny, with sparse and indistinct punctures; longer than transverse 45 110 view (Figure 3E). Second to third tergites with median longitudinal eye diameter in lateral view. Vertex and frons almost shiny, with 46 111 carina; first to third tergites with lateral carinae distinctly in lateral indistinct sparse punctures. Frons flat. Antenna slightly longer than 47 112 view. head and mesosoma combined, with 33 flagellomeres; each flag- 48 113 Material examined. South Korea: [GG] 2\\, Osan-si, Sucheong- ellomere longer than wide. First flagellomere 1.9 times as long as 49 114 dong, Mulhyanggi Arboretum, alt. 38 m, 16 ive31 viii 2012 (YD maximum width, approximately as long as third flagellomere. Oc- 50 115 Kwon & YM Ji). cipital carina complete. 51 116 Distribution. South Korea (new record), Japan, Russia (Primor’ye Mesosoma. Pronotum smooth and shiny, upper-posterior 52 117 Kray). portion with dense shallow punctures and hairs (Figure 4C). Epo- 53 118 mia indistinct. Mesoscutum with dense distinct fine punctures, 54 Trieces koreanus Choi & Lee, sp. nov. 119 posterior flat. Notaulus absent (Figure 4D). Scutellum weakly 55 Material examined. Holotype: male; Type depository: YNU. 120 convex, with sparse, indistinct punctures; lateral carina reaching 56 South Korea: Seoul, Korea University, Imeupsisukwan A, 24 vi 1987 121 apex. Mesopleuron fovea vestigial; lower posterior portion with 57 (JW Lee). 122 transverse wrinkles. Upper end of epicnemial carina reaching 58 Description. Holotype: male. 123 anterior margin of mesopleuron. Metapleuron almost flat, shiny, 59 Color. Black. Palpi and projection of upper face pale yellow 124 posterodorsal portion with few hairs, lower posterior portion with 60 (Figure 4B). Fore and mid legs yellowish brown. Hind tibia and tarsi 125 distinct wrinkles; with vertical slit-shaped depression posteriorly. 61 brown (Figure 4A). 126 Juxtacoxal carina strong. Wings slightly hyaline. Fore wing without 62 Head. Inner margins of eyes distinctly convergent downward. 127 areolet. Vein Cu-a distal of RsþM. Vein cu-a of hind wing incom- 63 Head widened posteriorly behind eyes in dorsal view. Face with 128 plete. Hind wing with five distal hamuli. Hind tibia gradually 64 weak sparse punctures, distance between punctures approximately 129 widened apically. Posterior spur of hind tibia approximately 2.7 65 as long as diameter of punctures. Apical margin of clypeus almost 130

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1 times as long as anterior spur. Ratio of length of hind tarsomeres References 33 2 33:15:12:10:20. Propodeum with strong apical transverse and lat- 34 3 eromedian longitudinal carinae. Propodeal spiracle large and cir- Aubert JF. 1963. Description de cinq espèces nouvelles appartenant aux genres 35 Lathrolestes, Phobetellus, Orthocentrus, Proclitus et Stethoncus (Hym., Ichneu- 4 cular, fused pleural carina. monidae). Bulletin de la Société Entomologique de Mulhouse 1963:82e86. 36 5 Metasoma. First tergite approximately 1.7 times as long as apical Baltazar CR. 1964. The genera of parasitic Hymenoptera in the Philippines, Part 2. 37 6 width, with dense punctures; median dorsal, dorsolateral, ventro- Pacific Insects 6:15e67. 38 7 Förster A. 1869. Synopsis der Familien und Gattungen der Ichneumonen. Ver- 39 lateral, and sublateral longitudinal carinae complete. Second tergite handlungen des Naturhistorischen Vereins der Preussischen Rheinlande und 8 densely punctate, median and sublateral carinae complete Westfalens 25:135e221. 40 9 (Figure 4E). Third tergite with median longitudinal carina until Gauld ID, Sithole R, Gómez JU, et al. 2002. The Ichneumonidae of Costa Rica: 4. 41 e 10 basal 0.7; basal 0.5 with lateral longitudinal carinae. Basal half of Memoirs of the American Entomological Institute 66:1 768. 42 Haliday AH. 1838. Descriptions of new British insects, indicated in Mr. Curtis’s 11 fourth tergite densely punctate. guide. Annals of Natural History 2:112e121. 43 12 Distribution. South Korea. Heinrich GH. 1949. Neue und interessante Schlupfwespen aus Bayern (Hym.). 44 e e 13 Etymology. The specific name is derived from the holotype Mitteilungen Münchener Entomologischen Gesellschaft 35 39:101 127. 45 Holmgren AE. 1856. Entomologiska anteckningar under en resa i södra Sverige ar 14 locality. 1854. Kongliga Svenska Vetenskapsakademiens Handlingar 75:1e104. 46 15 Remarks. This new species is similar to T. homonae Kusigemati Kusigemati K. 1967. Descriptions of four new species of the genus Trieces Townes 47 e 16 and can be distinguished from the latter by the following combi- from Japan (Hymenoptera: Ichneumonidae). Insecta Matsumurana 29:45 49. 48 Kusigemati K. 1984. Metopiinae (Hymenoptera, Ichneumonidae) from Mongolia. 17 nation of characters: antenna slightly longer than head and mes- Annales Historico-Naturales Musei Nationalis Hungarici 76:245e263. 49 18 osoma combined, with 33 flagellomeres; head widened posteriorly Latreille PA. 1802. Histoire naturelle, générale et particulière, des Crustacés et des 50 19 behind eyes in dorsal view; temple convex, longer than transverse Insectes. Tome troisième. Paris. 468 pp. (Ichneumonidae, pp. 318e327). Q6 51 Tolkanitz VI. 2010. Ichneumon flies of the genus Trieces (Hymenoptera, Ichneu- 20 eye diameter in lateral view; face black with yellow projection. monidae, Metopiinae) in the Palaearctic fauna. Entomological Review 90:465e 52 21 472. http://dx.doi.org/10.1134/S001387381004007X. 53 22 Q5 Acknowledgments Townes HK. 1946. The generic position of the Neotropic Ichneumonidae with types 54 23 in the Philadelphia and Quebec museums described by Cresson, Hooker, Nor- 55 ton, Provancher, and Viereck. Boletín de Entomologia Venezolana 5:29e63. 24 We are deeply grateful to anonymous reviewers for reviewing Townes HK, Townes M. 1959. Ichneumon-flies of America north of Mexico: 1 56 25 this manuscript. We thank Professor Yanko Kolarov from the Fac- Subfamily Metopiinae. United States National Museum Bulletin 216:1e318. 57 26 ulty of Pedagogie, University of Plovdiv, Bulgaria, for providing Yu DS, Van Achterberg C, Horstmann K. 2012. Taxapad 2012, World Ichneumo- 58 noidea 2011. Taxonomy, Biology, Morphology and Distribution. Database on USB 27 useful comments. This work was supported by a grant from the flash-drive. Available at: www.taxapad.com, Ottawa, Ontario, Canada. Q7 59 28 National Institute of Biological Resources (NIBR), funded by the Zhang Y, Sheng M-L, Xiong Z-C. 2016. A new species of Metopiinae (Hymenoptera, 60 e 29 Ministry of Environment (MOE) of the Republic of Korea Ichneumonidae) parasitizing lepidopteran larvae in China. ZooKeys 572:71 79. 61 http://dx.doi.org/10.3897/zookeys.572.8031. 30 (NIBR201601207). 62 31 63 32 64