Product Datasheet

RGS14 Antibody

Catalog No: #34982

Package Size: #34982-1 50ul #34982-2 100ul Orders: [email protected] Support: [email protected]

Description

Product Name RGS14 Antibody

Host Species Rabbit

Clonality Polyclonal

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific

immunogen.

Applications WB

Species Reactivity Hu Ms

Specificity The antibody detects endogenous levels of total RGS14 .

Immunogen Type Peptide

Immunogen Description Synthesized peptide derived from internal of human RGS14.

Target Name RGS14

Other Names regulator of G-protein signaling 14; RGSE;

Accession No. Swiss-Prot: O43566NCBI ID: 10636

SDS-PAGE MW 65kd

Concentration 1.0mg/ml

Formulation Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide

and 50% glycerol.

Storage Store at -20°C

Application Details

Western blotting: 1:500~1:3000

Images

Western blot analysis of extracts from NIH/3T3 cells, using RGS14 antibody #34982.

Background

Acts as a regulator of signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. Lopez-Aranda M.F., Eur. J. Neurosci. 23:2971-2982(2006). Chatterjee T.K., Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases. Puhl H.L. III, Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.

Note: This product is for in vitro research use only and is not intended for use in humans or animals.

Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2