CD4+ T Cell Di erentiation

CD69 CD94 CD150 (SLAMF1) CD160 (Ms) Surface Markers Thymus CD161 (Ms) Co Ad CD314 (NKG2D) CD44 IL-15 Ly-108 CD49b TIM-3 (CD366) CD56 (N-CAM) Co Ad

I n rs tr e Cy ac rk Regulation of Ch ellular Ma immune responses, CD45RA CD45RB CD44lo immune tolerance VISTA (PD-1H) NKT CD62L (L-Selectin) E ector Co Ad Cy Cytokines IL-15Rα CD39 PLZF CD45RA CD73 Surface Markers CD152 (CTLA-4) CD103 CD279 (PD-1) Ad Initiation of Co Costimulatory or Coinhibitory Molecules TIM-3 (CD366) (Ms) Naïve TCR Ligation IL-4 Co + IL-17A immunity, Ad Adhesion Molecules APC Costimulation IFN-γ regulation

Cy Cytokines or Cytokine Receptors Th F

o Ch Chemokine Receptors 5 x T Cy P nTreg A Ch CD127 3 T

Activators • S Cy CD184 (CXCR4) Ch H • hi CD197 (CCR7) e 3 CD25 CD197 (CCR7) l d lo ios • Sma CD127 CD134 (OX-40) IL-2 Inhibitors CD137 (4-1BB) CD69hi Immunity CD304 (Nrp1) CD71 hi to microbial TGF-β FolR4/FR4 (Ms) CD98 Th22 Hu = Human IL-10 GARP HLA-DR pathogens Mouse IL-35 GITR LAG-3 (CD223) Ms = LAP TIGIT hi IL-6, TNF CD44 Ch Cy APC = Antigen Presenting Cell TIM-1 (CD365) Ad AhR hi CD194 (CCR4) CD140 (PDGFR) TIM-3 (CD366) CD25 CD196 (CCR6) VISTA (PD-1H) Cy CCR10 Co IL-22 Activated TGF-β TNF CD152 (CTLA-4) F IL-1, IL-6, IL-21, IL-23, TGF-β CD223 (LAG3) o B CD161 (NK1) x 7 CD279 (PD-1) P Th B CD278 (ICOS) CD39 3 T TIM-3 (CD366) • B TIM-3 (CD366)

CD73 K • Z

Co CD103 i-67 Co

IL-2, Retinoic Acid, TGF-

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IFN-γ, IL-2, IL-4, IL-12, IL-27 -

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IL-2, IL-12 (p70), IL-18, IL-27, RANKL •

Regulation IL-2, IL-4, IL-25, IL-31, IL-33, TSLP •

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o R 4 iTreg IL-1 O of tolerance F x h R P R A IL-21 α I 3 Th17 4 • • IL-23 • Cy • f S 5 t m AT hi S a Ch ad3 • ST CD25 TA B Variable CD127lo T3 1 • Ch • Runx Cy expression CD134 (OX-40) IFN-γ, TGF-β CD26 CD194 (CCR4) CD126 (IL-6Rα) CD137 (4-1BB) IL-4, IL-10, IL-21 IL-6, IL-21, CXCL13 CD94 CD196 (CCR6) IL-17A IL-13Rα1 IL-10 FolR4/Fr4 (Ms) CD278 (ICOS) IL-17F IL-21R IL-35 GARP Tim-3 (CD366) IL-17A/F IL-23R TGF-β GITR Co IL-21 LAP IL-4, TGF-β IFN-γ IL-22 CD84 (SLAMF5) IL-24 Immunity to CD150 (SLAMF1) IL-26 (Hu) CD200 (OX-2) TNF extracellular CD272 (BTLA) CCL20 (MIP-3α) bacterial and CD278 (ICOS) CD279 (PD-1) fungal infections T CD57 - Th1 3 TIGIT Tim-1 (CD365) b x Co Ad e n t u

Co • R

G

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3

c CD183 (CXCR3)

CD178 (FasL)

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• CD195 (CCR5)

CD212 (IL-12Rβ2)

S IFN-

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T F CD218a (IL-18Rα) TGF-β A LT-α Tfh Th2 R T I IL-27Rα

B TNF G 5

3 • NOTCH3 c 4

Ch l T A • Perforin - Cy F 1

6 A T S - RANKL

CD185 (CXCR5) T R I Granzyme A • S A T F M CD126 (IL-6Rα) I A • G AF 3 T6 • Cy Granzyme B CD153 (CD30L) • Ch • F MA ( CD30 Granulysin (Hu) e 1 CD154 (CD40L) . CD184 (CXCR4) a Th9 IFN-γRβ Immunity to IFN- γ r U CD304 (Nrp1) l CD193 (CCR3) T-cell help IL-4 y P IL-4 IL-17RB IL-21R ) • CD194 (CCR4) viruses, IL-17A • IL-5 IL-33Rα for B cells S T6 Cy CD198 (CCR8) IL-17F MA TA IL-6 NOTCH1 Ds • S VDR (Ms) CD294 (CRTH2) intracellular IL-21 IL-10 NOTCH2 IL-13 bacteria, IL-9 IL-31 and parasites IL-10 Immunity to CCL17 (TARC) Immunity to CCL22 (MDC) extracellular extracellular TGF-β parasites T cells play central roles in cell-mediated and humoral immunity. Several parasites di erent subsets of T cells have been discovered, each with distinct functions. Each subset has a characteristic cytokine signature that can be measured at the single-cell level using multicolor flow cytometry, combining surface phenotyping and intracellular staining of cytokines, transcription factors, or signaling molecules. Additionally, multiple soluble cytokines or signaling molecules can be quantitated simultaneously using BD™ Cytometric Bead Array (CBA) technology. Other complementary technologies to further the research in the T cell area include BD OptEIA™ ELISA reagents for cytokine quantitation and BD™ ELISPOT reagents for determining the frequency of cytokine production in cells. BD Life Sciences For Research Use Only. Not for use in diagnostic or therapeutic procedures. © 2016 BD. BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Biosciences 23-10639-03 bdbiosciences.com/tcell bdbiosciences.com