Buffered Glucose Broth (Mr - Vp Medium) Tm 324

www.tmmedia.in PRODUCT DATA SHEET BUFFERED GLUCOSE BROTH (MR - VP MEDIUM) TM 324

INTENDED USE Used in differentiation of bacteria by MR-VP test

COMPOSITION

Ingredients Gms/L Monopotassium phosphate 5.000

Dextrose 5.000 Pancreatic digest of casein 3.500

Peptic digest of animal tissue 3.500

PRODUCT SUMMARY AND EXPLAINATION

BUFFERED GLUCOSE BROTH (MR – VP MEDIUM) is used for the differentiation of Colon- aerogenes family of bacteria could be divided into two groups based on their action in a peptone and dextrose medium. The MR and VP tests appear in the identification scheme for Enterobacteriaceae, important isolates in clinical microbiology and food and dairy microbiology testing. MR-VP Broth is also known as Methyl Red-Voges-Proskauer Medium. When tested with the pH indicator methyl red, the “coli” group produced high acidity while the “aerogenes” group produced a less acid reaction. The test to detect high-acid end products is known as the Methyl Red (MR) test. A color reaction occurs when certain cultures, incubated in a medium containing peptone and dextrose, are treated with potassium hydroxide and exposed to air.

PRINCIPLE Methyl red-positive organisms produce high levels of acid during fermentation of dextrose, overcome the phosphate buffer system and produce a red color upon the addition of the methyl red pH indicator. In the Voges-Proskauer test, the red color produced by the addition of potassium hydroxide to cultures of certain microbial species is due to the ability of the organisms to produce a neutral end product, acetoin (acetylmethylcarbinol), from the fermentation of dextrose. The acetoin is oxidized in the presence of oxygen and alkali to produce a red color. This is a positive Voges- Proskauer reaction.

Manufacturer Address: Titan Biotech Limited, A- 902A, RIICO Industrial Area, Phase III, Bhiwadi-301019. Authorized Representative: MedNet GmbH, Borkstrasse 10, 48163 Munster, Germany. Page 1 www.tmmedia.in PRODUCT DATA SHEET INSTRUCTIONS FOR USE 1. Dissolve 17g in 1000ml of distilled water. 2. Gently heat to dissolve the medium completely. 3. Distribute 10 ml of amount in each test tube and autoclave at 15 psi (at 121°C) for 15 minutes. 4. Cool to room temperature prior to use.

QUALITY CONTROL SPECIFICATIONS Appearance Dehydrated powder: Cream to yellow colour, free flowing powder Appearance of the prepared medium: Light yellow colour, clear solution pH (at 25°C): 6.9 ± 0.2

INTERPRETATION Cultural characteristics observed incubation at 35 ± 2°C for 48 hours.

Inoculum Growth Test Result Test strains ATCC (CFU) MR Test VP Test

Escherichia coli 25922 87 Luxuriant + (Red) - (No change)

Klebsiella pneumoniae 23357 80 Luxuriant - (Yellow) + (Red)

Enterobacter aerogenes 13048 83 Luxuriant - (Yellow) + (Red)

REFERENCES 1. Clark, W.M. and Lubs, H.A. 1915. J. Infect. Dis. 17:160-173. 2. Voges, O. and Proskauer, B. 1898. Z. Hyg. 28:20-22. 3. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., Yolken, R.H. (Eds.). 1995. Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C. 4. Vanderzant, C. and Splittstoesser, D.F. (Eds.). 1992. Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, Washington, D.C. 5. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. I. Williams & Wilkins, Baltimore, Md.

NOTE: Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices

Manufacturer Address: Titan Biotech Limited, A- 902A, RIICO Industrial Area, Phase III, Bhiwadi-301019. Authorized Representative: MedNet GmbH, Borkstrasse 10, 48163 Munster, Germany. Page 1