MTF1 Antibody Cat. No.: 27-481

MTF1 Antibody

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Dog, Human, Mouse, Rat

Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human MTF1.

TESTED APPLICATIONS: ELISA, WB

MTF1 antibody can be used for detection of MTF1 by ELISA at 1:2500. MTF1 antibody can APPLICATIONS: be used for detection of MTF1 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate

PREDICTED MOLECULAR 81 kDa WEIGHT:

Properties

PURIFICATION: Antibody is purified by protein A chromatography method.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

October 3, 2021 1 https://www.prosci-inc.com/mtf1-antibody-27-481.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose.

CONCENTRATION: batch dependent

For short periods of storage (days) store at 4˚C. For longer periods of storage, store MTF1 STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Additional Info

OFFICIAL SYMBOL: MTF1

ALTERNATE NAMES: MTF1, ZRF, MTF-1

ACCESSION NO.: NP_005946

PROTEIN GI NO.: 112790163

GENE ID: 4520

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The zinc finger transcription factor MTF-1 (metal-responsive transcription factor-1) is conserved from insects to vertebrates. The major role of MTF-1 in both organisms is to control the transcription of genes involved in the homeostasis and detoxification of heavy metal ions such as Cu2+, Zn2+ and Cd2+. In mammals, MTF-1 serves at least two BACKGROUND: additional roles. First, targeted disruption of the MTF-1 gene results in death at embryonic day 14 due to liver degeneration, revealing a stage-specific developmental role. Second, under hypoxic-anoxic stress, MTF-1 helps to activate the transcription of the gene placental growth factor (PIGF), an angiogenic protein.

REFERENCES: 1) Chen, X., et al., (2004) J. Biol. Chem. 279 (6), 4515-4522.

ANTIBODIES FOR RESEARCH USE ONLY.

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