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Bio-Analytical Study of Plants Used in Traditional Medicine in Togo Nassifatou Koko Tittikpina

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Bio-Analytical Study of Plants Used in Traditional Medicine in Togo Nassifatou Koko Tittikpina Bio-analytical study of plants used in traditional medicine in Togo Nassifatou Koko Tittikpina To cite this version: Nassifatou Koko Tittikpina. Bio-analytical study of plants used in traditional medicine in Togo. Analytical chemistry. Université de Lorraine; Universität des Saarlandes, 2017. English. NNT : 2017LORR0169. tel-01777427 HAL Id: tel-01777427 https://tel.archives-ouvertes.fr/tel-01777427 Submitted on 24 Apr 2018 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. AVERTISSEMENT Ce document est le fruit d'un long travail approuvé par le jury de soutenance et mis à disposition de l'ensemble de la communauté universitaire élargie. Il est soumis à la propriété intellectuelle de l'auteur. Ceci implique une obligation de citation et de référencement lors de l’utilisation de ce document. D'autre part, toute contrefaçon, plagiat, reproduction illicite encourt une poursuite pénale. Contact : [email protected] LIENS Code de la Propriété Intellectuelle. articles L 122. 4 Code de la Propriété Intellectuelle. articles L 335.2- L 335.10 http://www.cfcopies.com/V2/leg/leg_droi.php http://www.culture.gouv.fr/culture/infos-pratiques/droits/protection.htm BIO-ANALYTICAL STUDY OF PLANTS USED IN TRADITIONAL MEDICINE IN TOGO Dissertation zur Erlangung des Grades des Doktors der Naturwissenschaften der Naturwissenschaftlich-Technischen Fakultät der Universität des Saarlandes von Nassifatou Koko Tittikpina Saarbrücken 2017 Thèse Présentée en vue de l’obtention du grade de DOCTEUR DE L’UNIVERSITE DE LORRAINE Mention : Chimie Analytique Par Nassifatou Koko Tittikpina BIO-ANALYTICAL STUDY OF PLANTS USED IN TRADITIONAL MEDICINE IN TOGO Soutenue le 19 Septembre 2017 devant la commission d’examen JURY Université de Kaiserslautern Prof. Dr. Richling Elke Rapporteur – Professeur (Allemagne) Dr. Vonthron- Rapporteur - Maître de Université de Strasbourg (France) Sénécheau Catherine conferences HDR Prof. Dr. Duval Raphaël Examinateur - Professeur Université de Lorraine (France) Prof. Dr. Müller Uli Examinateur - Professeur Université de la Sarre (Allemagne) Examinateur - Maître de Dr. SPINA Rosella Université de Lorraine (France) conferences Examinateur – Chercheur Dr. Kessler Sonja Université de la Sarre (Allemagne) Post-doctoral Prof. Dr. Chaimbault Co-Directeur de thèse Université de Lorraine (France) Patrick Prof. Dr. Jacob Claus Directeur de thèse Université de la Sarre (Allemagne) Tag des Kolloquiums: 19. 09. 2017 Dekan: Prof. Dr. rer. nat. Guido Kickelbick Prüfungsvorsitzender: Prof. Dr. Ingolf Bernhardt Berichterstatter: Prof. Dr. Claus Jacob Prof. Dr. Uli Müller Akad. Mitarbeiterin: Dr. Sonja Kessler Weitere Mitglieder: Prof. Dr. Chaimbault Patrick Prof. Dr. Richling Elke Prof. Dr. Duval Raphaël Dr. Vonthron-Sénécheau Catherine Dr. Spina Rosella Diese Dissertation wurde in der Zeit von Oktober 2014 bis Juli 2017 unter Anleitung von Prof. Dr. Claus Jacob im Arbeitskreis für Bioorganische Chemie, Fachrichtung Pharmazie der Universität des Saarlandes und Prof. Dr. Patrick Chaimbault im Arbeitkreis Laboratoire de Structure et Réactivité des Systèmes Moléculaires Complexes SRSMC (UMR 7565), Fachrichtung Chemie der Université de Lorraine (Frankreich) durchgeführt. Bei Herr Prof. Dr. Claus Jacob und Herr Prof. Dr. Patrick Chaimbault möchte ich mich für die Überlassung des Themas und die wertvollen Anregungen und Diskussionen herzlich bedanken. To You, Who is always by my side Dedicated to my beloved mother (my inspiration) Table of Contents Table of Contents ......................................................................................................................... Acknowledgments ....................................................................................................................... i Abbreviations ............................................................................................................................ iii Zusammenfassung ...................................................................................................................... v Summary ................................................................................................................................... vii Allgemeine Zusammenfassung ................................................................................................. ix I. Allgemeine Einleitung ........................................................................................................ x II. Ethnobotanische Studie ................................................................................................... xiii III. Biologischer Teil ........................................................................................................... xviii IV. Analytischer Teil ............................................................................................................ xxii V. Pharmazeutische Technologie, Nanopulverisieren von Pflanzen: Nanosized................ xxv Natural Pflanzenmaterial von Pterocarpus erinaceus Poir mit vielversprechenden…………… antibakteriellen Aktivitäten .................................................................................................... xxv VI. Schlussfolgerung und Perspektiven .............................................................................. xxvii Résumé général de la thèse .................................................................................................... xxix 1. Introduction générale ...................................................................................................... xxx 2. Enquête ethnobotanique .............................................................................................. xxxiii 3. Partie biologique ........................................................................................................ xxxviii 4. Partie analytique .............................................................................................................. xlii 5. Technologie pharmaceutique, nanoparticules de poudres végétales : .................................. nanoparticules d’écorce de tronc de Pterocarpus erinaceus Poir aux activités antibiotiques….. prometteuses ............................................................................................................................ xlv 6. Conclusion et perspectives ............................................................................................ xlvii General Introduction ................................................................................................................... 1 1. General characteristics ........................................................................................................ 2 1.1. Climate and vegetation ............................................................................................. 2 1.2. Pedology and hydrography....................................................................................... 3 1.3. Population................................................................................................................. 3 2. Healthcare ........................................................................................................................... 5 Chapter 1: Ethnobotanical study of plants used in the Tchamba district of Togo to treat……… infectious diseases .................................................................................................................... 13 1. Introduction ...................................................................................................................... 12 2. Selection of a suitable area for an ethnobotanical survey: the Tchamba District of ........ 13 3. Recording of traditional knowledge and data collection .................................................. 14 3.1. Turning testimonies into data ................................................................................. 15 3.2. CAPITURE of the most relevant plants based on calculated values ..................... 17 4. Results .............................................................................................................................. 20 Description of Pterocarpus erinaceus Poir ...................................................................... 28 Description of Daniellia oliveri (Rolfe) Hutch. et Dalz ................................................... 29 5. Discussion ......................................................................................................................... 30 6. Conclusions ...................................................................................................................... 33 Chapter 2: Biological activities of Pterocarpus erinaceus and Daniellia oliveri .................. 35 1. Introduction ...................................................................................................................... 36 2. Collection and preparation of plant material .................................................................... 37 3. Extraction and fractionation ............................................................................................
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