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Vaginitis and Abnormal Vaginal Bleeding
UCSF Family Medicine Board Review 2013 Vaginitis and Abnormal • There are no relevant financial relationships with any commercial Vaginal Bleeding interests to disclose Michael Policar, MD, MPH Professor of Ob, Gyn, and Repro Sciences UCSF School of Medicine [email protected] Vulvovaginal Symptoms: CDC 2010: Trichomoniasis Differential Diagnosis Screening and Testing Category Condition • Screening indications – Infections Vaginal trichomoniasis (VT) HIV positive women: annually – Bacterial vaginosis (BV) Consider if “at risk”: new/multiple sex partners, history of STI, inconsistent condom use, sex work, IDU Vulvovaginal candidiasis (VVC) • Newer assays Skin Conditions Fungal vulvitis (candida, tinea) – Rapid antigen test: sensitivity, specificity vs. wet mount Contact dermatitis (irritant, allergic) – Aptima TMA T. vaginalis Analyte Specific Reagent (ASR) Vulvar dermatoses (LS, LP, LSC) • Other testing situations – Vulvar intraepithelial neoplasia (VIN) Suspect trich but NaCl slide neg culture or newer assays – Psychogenic Physiologic, psychogenic Pap with trich confirm if low risk • Consider retesting 3 months after treatment Trichomoniasis: Laboratory Tests CDC 2010: Vaginal Trichomoniasis Treatment Test Sensitivity Specificity Cost Comment Aptima TMA +4 (98%) +3 (98%) $$$ NAAT (like GC/Ct) • Recommended regimen Culture +3 (83%) +4 (100%) $$$ Not in most labs – Metronidazole 2 grams PO single dose Point of care – Tinidazole 2 grams PO single dose •Affirm VP III +3 +4 $$$ DNA probe • Alternative regimen (preferred for HIV infected -
Original Article
ORIGINAL ARTICLE ASSOCIATION OF BACTERIAL VAGINOSIS WITH PRETERM LABOUR Shilpa M.N1, A.P. Chandrashekar2, G.S. Vijay Kumar3, Rashmi P. Mahale4 HOW TO CITE THIS ARTICLE: Shilpa M.N, A.P Chandrashekar, G.S. Vijay Kumar, Rashmi P. Mahale. “Association of bacterial vaginosis with preterm labour”. Journal of Evolution of Medical and Dental Sciences 2013; Vol2, Issue 32, August 12; Page: 6104-6110. ABSTRACT: OBJECTIVES: To study the prevalence of bacterial vaginosis (BV) in preterm labour and to investigate its association as one of the causative factors of preterm labour. MATERIALS AND METHODS: Fifty women who presented with preterm labour (study group) and fifty women in labour at term (control group) admitted to a teaching hospital from November 2009 to May 2011 were examined for bacterial vaginosis using Nugent score. All the statistical methods were carried out through SPSS for windows (version 16.0), STUDY DESIGN: A comparative study. RESULTS: The prevalence of bacterial vaginosis among preterm labour group was 22% and its prevalence among full term group was 4%. There was statistically significant association of BV with preterm labor when compared to term labor (p=.007). Bacterial Vaginosis was strongly associated with very preterm delivery (<34 weeks) (p=.050). Bacterial vaginosis was significantly associated with lesser gestational age at delivery and low birth weight. CONCLUSION: Bacterial vaginosis is significantly associated with preterm labour and is one of the probable causative factors of preterm labour. KEYWORDS: Bacterial vaginosis, Nugent score, Preterm labor. INTRODUCTION: Preterm labour is defined as onset of labour prior to completion of 37 weeks gestation after period of viability.1,2 Though only 7-10% of all deliveries are preterm, prematurity alone accounts for more than 80% of perinatal morbidity and mortality in India.1,2,3,4 The etiology for preterm labour is multifactorial and in many of the cases is obscure. -
Congenital Uterine Anomalies: the Role of Surgery Maria Carolina Fernandes Lamouroux Barroso M 2021
MESTRADO INTEGRADO EM MEDICINA Congenital uterine anomalies: the role of surgery Maria Carolina Fernandes Lamouroux Barroso M 2021 Congenital uterine anomalies: the role of surgery Dissertação de candidatura ao grau de Mestre em Medicina, submetida ao Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto Maria Carolina Fernandes Lamouroux Barroso Aluna do 6º ano profissionalizante de Mestrado Integrado em Medicina Afiliação: Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto Endereço: Rua de Jorge Viterbo Ferreira nº228, 4050-313 Porto Endereço eletrónico: [email protected]; [email protected] Orientador: Dra. Márcia Sofia Alves Caxide e Abreu Barreiro Diretora do Centro de Procriação Medicamente Assistida e do Banco Público de Gâmetas do Centro Materno-Infantil do Norte Assistente convidada, Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto. Afiliação: Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto Endereço: Largo da Maternidade de Júlio Dinis 45, 4050-651 Porto Endereço eletrónico: [email protected] Coorientador: Prof. Doutor Hélder Ferreira Coordenador da Unidade de Cirurgia Minimamente Invasiva e Endometriose do Centro Materno- Infantil do Norte Professor associado convidado, Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto. Afiliação: Instituto de Ciências Biomédicas Abel Salazar – Universidade do Porto Endereço: Rua Júlio Dinis 230, B-2, 9º Esq, Porto Endereço eletrónico: [email protected] Junho 2021 Porto, junho de 2021 ____________________________________ (Assinatura da estudante) ____________________________________ (Assinatura da orientadora) ____________________________________ (Assinatura do coorientador) ACKNOWLEDGEMENTS À Dra. Márcia Barreiro, ao Dr. Luís Castro e ao Prof. Doutor Hélder Ferreira, por toda a disponibilidade e empenho dedicado à realização deste trabalho. Aos meus pais, irmão e avós, pela participação que desde sempre tiveram na minha formação, e pelo carinho e apoio incondicional. -
Evaluation of Different Diagnostic Methods of Bacterial Vaginosis
IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) e-ISSN: 2279-0853, p-ISSN: 2279-0861. Volume 13, Issue 1, Ver. VIII (Feb. 2014), PP 15-23 www.iosrjournals.org Evaluation of different diagnostic methods of bacterial vaginosis Gamal F. M. Gad¹, Ahmed R. El-Adawy², Mohammed S. Mohammed3, Abobakr F. Ahmed1, Heba A. Mohamed¹ ¹ Microbiology department, Faculty of Pharmacy, Minia University 2 Gynecology and Obstetrics department, Faculty of Medicine, Minia University 3 Microbiology department, Faculty of Medicine, Minia University Abstract: Bacterial vaginosis (BV) is an ecological disorder of the vaginal microbiota that affects millions of women annually, and is associated with numerous adverse health outcomes including pre-term birth and the acquisition of sexually transmitted infections. This study aimed to determine the incidence rate of bacterial vaginosis among women and examine the effect of some risk factors and demographic features on BV incidence. Also aimed to compare between the different methods of BV diagnosis. The results revealed that from the 100 patients, 33% were diagnosed as bacterial vaginosis, 23% were having candidiasis, 8% aerobic vaginitis, 3% trichomoniasis and 33% were normal. The highest number of BV positive cases was in the age group of (26-35)Yrs, 94% of the 33 BV positive cases were using vaginal douches and 94% were married. BV positive cases using IUD were equal to BV positive cases using hormonal contraceptives including injection and tablets (12/33, 36.4%). BV was diagnosed in 33%, 38%, 36%, 30% and 34% of patients using Gram stain, culture, Amsel criteria, BV Blue test® and qPCR respectively. -
Diagnosing Bacterial Vaginosis with a Novel, Clinically-Actionable Molecular Diagnostic Tool
ISSN: 2581-7566 Volume 1: 2 J Appl Microb Res 2018 Journal of Applied Microbiological Research Diagnosing Bacterial Vaginosis with a Novel, Clinically-Actionable Molecular Diagnostic Tool Joseph P Jarvis1 Doug Rains2 1Coriell Life Sciences, Pennsylvania, USA Steven J Kradel1 2Quantigen Genomics, Indiana, USA James Elliott2 3ThermoFisher Scientific, USA Evan E Diamond3 4Primex Clinical Laboratories, California, USA Erik Avaniss-Aghajani4 5Maternity & Infertility Institute, California, USA Farid Yasharpour5 Jeffrey A Shaman1* Abstract Article Information Bacterial vaginosis is a common condition among women of Article Type: Research reproductive age and is associated with potentially serious side-effects, Article Number: JAMBR109 including an increased risk of preterm birth. Recent advancements in Received Date: 15 June, 2018 microbiome sequencing technologies have produced novel insights Accepted Date: 11 July, 2018 into the complicated mechanisms underlying bacterial vaginosis and Published Date: 18 July, 2018 have given rise to new methods of diagnosis. Here we report on the validation of a quantitative, molecular diagnostic algorithm based on *Corresponding author: Dr. Jeffrey A Shaman, Coriell the relative abundances of ten potentially pathogenic bacteria and four Life Sciences, Philadelphia, Pennsylvania, USA. Tel: + 609 357 0578; Email: jshaman(at)coriell.com as symptomatic (n=149) or asymptomatic (n=23). We observe a clear #These Authors are Joint First Authors on this Work. commensal Lactobacillus species in research subjects (n=172) classified and reinforcing pattern among patients diagnosed by the algorithm that Citation: Jarvis JP, Rains D, Kradel SJ, Elliott J, Diamond is consistent with the current understanding of biological dynamics EE, Avaniss-Aghajani E, Yasharpour F, Shaman JA (2018) and dysregulation of the vaginal microbiome during infection. -
Development and Validation of a Model for Individualized Prediction
Wu et al. Reproductive Biology and Endocrinology (2021) 19:6 https://doi.org/10.1186/s12958-020-00693-x RESEARCH Open Access Development and validation of a model for individualized prediction of cervical insufficiency risks in patients undergoing IVF/ICSI treatment Yaoqiu Wu1,2, Xiaoyan Liang1, Meihong Cai3, Linzhi Gao1, Jie Lan2 and Xing Yang1* Abstract Background: Women who conceived with in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) are more likely to experience adverse pregnancy outcomes than women who conceived naturally. Cervical insufficiency (CI) is one of the important causes of miscarriage and premature birth, however there is no published data available focusing on the potential risk factors predicting CI occurrence in women who received IVF/ICSI treatment. This study aimed to identify the risk factors that could be integrated into a predictive model for CI, which could provide further personalized and clinically specific information related to the incidence of CI after IVF/ICSI treatment. Patients and methods: This retrospective study included 4710 patients who conceived after IVF/ICSI treatment from Jan 2011 to Dec 2018 at a public university hospital. The patients were randomly divided into development (n = 3108) and validation (n = 1602) samples for the building and testing of the nomogram, respectively. Multivariate logistic regression was developed on the basis of pre-pregnancy clinical covariates assessed for their association with CI occurrence. Results: A total of 109 patients (2.31%) experienced CI among all the enrolled patients. Body mass index (BMI), basal serum testosterone (T), gravidity and uterine length were associated with CI occurrence. The statistical nomogram was built based on BMI, serum T, gravidity and uterine length, with an area under the curve (AUC) of 0.84 (95% confidence interval: 0.76–0.90) for the developing cohort. -
Fallopian Tubes
Joy of GettinG PreGnant Your Complete Guide To Overcome Infertility author / editor / Copyright © Dr. Rupal N. Shah, M.D., D.G.O., Diploma in Reproductive Medicine (Kiel), Germany Contact: [email protected] Publisher: Pugmarks Mediaa, 119, Swami Vivekanand Marg, Allahabad - 211003 (UP) first edition : 2013 Second edition : 2017 Concept / Layout Pugmarks Mediaa & Team IVF India available at: BlOSSOM FeRTIlITY & TeST TUBe BABY CeNTRe Sutaria Building, Near Bahumali Building, Nanpura, Surat - 395001, Gujarat, India Phone : 0261-2470333, 2470444, +91 99799 46222 email : info@blossomivfindia.com Website: www.blossomivfindia.com Cover: Raj Bhagat Printing: Vavo Prints, Bai-ka-Bagh, Allahabad iSBn: 978-81-932743-3-0 Price: Rs 250/- note: Contents of this booklet cannot be published elsewhere. The Opinions and contents of this booklet belongs to the doctor himself, and they are being published just to create awareness in the people. It should not be used by the patients for self diagnosis without medical advice. New therapeutics are updated due to technology and the contents of this booklet may be old or incomplete or with some errors and omissions. But purpose of this booklet is only public awareness and without Medical Ad - vice, no content of this booklet should be followed, as the composition of each patient is different, hence, it is not necessary that the content of this booklet is fit for your purpose. The patient will be responsible if this book is followed without any medical advice. If the content of this booklet is found with error or any injustice is found to be done by any one, he may kindly draw attention. -
Genetics of Recurrent and Spontaneous Miscarriage
Research and Reviews Journal of Medical and Health Science ISSN: 2319-9865 Genetics of Recurrent and Spontaneous Miscarriage Arka Dutta Gupta1* and Akash Baid2 1 Department of Human Genetics, Institute of Genetic Engineering, Madhyamgram, Badu, Itkhola, Kolkata, West Bengal 2 Department of Biotechnology, Institute of Genetic Engineering, Madhyamgram, Badu, Itkhola, Kolkata, West Bengal Research Article Received: 01/03/2017 ABSTRACT Revised: 20/03/2017 Accepted: 27/03/2017 The aim was to find out the genetic basis of recurrent spontaneous abortion (RSA) from the past pregnancies and ensure a more favourable *For Correspondence outcome in the current or future pregnancy. Pregnancy loss has always been Gupta AD, Department of Human a devastating experience for the mothers and the clinician of concern. One Genetics, Institute of Genetic out of four pregnancies ends in miscarriage. It is estimated that 50-60% of Engineering, Madhyamgram, all first trimester pregnancy losses are the cause of chromosomal Badu, Itkhola, Kolkata, West abnormality. Bengal, Tel: 8978764411. 8 couples were selected with the history of Recurrent Spontaneous fetal loss and were suggested ultrasound scan while no such abnormalities Keywords: Recurrent were found, hence further we asked for cytogenetic procedure for detection spontaneous miscarriage, Bad of chromosomal abnormalities by the method karyotyping. Obstetric History, chromosomal We discovered 45;X/46;XX, 45;X/46;XY and 46;XX/46;XY in female abnormalities partners and 46;XX/46;XY, 46;XY/47;XXY mosaicism in male partners. Two unique balanced translocations in females such as 46;XX,t(5q35:8q24) E-mail: and46;XX,t(14q:21q) and a single balanced translocation in male partner [email protected] 46;XY,t(6q:8p) with reproductive failure. -
Bacterial Vaginosis in Pregnancy and Risk of Spontaneous Preterm
Bacterial vaginosis in pregnancy and risk of spontaneous preterm delivery Approved at the obstetrical guideline-meeting January 2015 Danish Society of Obstetrics and Gynecology (DSOG) Update February 2019 The Lancet has now published reference 31b, by Subtil et al. (2018). The 2015 version of the DSOG guideline included this reference as a congress presentation (ref. 31a), i.e. the risk of bias was high. However, the DSOG working group finds that this publication supports the DSOG recommenda- tions from 2015 (see “Update February 2019” for details). Apart from the “Update February 2019” paper, we did not make any other changes to the original guideline from 2015 . Members of the working group • DSOG: Niels Uldbjerg (chairman, [email protected], +4520679420), Anne Ersbøll, Jens Svare, Kirstine Sneider, Rikke Bek Helmig, Thor Haahr, Mona Aarenstrup Karlsen, Lene Hee Christensen. • Midwife: Louise Weile (MHSc) • Clinical Pharmacology: Agnes Ziobrowska Bech, MD (Department of Clinical Pharmacology, Aarhus University Hospital) • Clinical Microbiology: Claus Østergaard MD, consultant (Department of Clinical Microbiology, Vejle, Denmark) • External reviewers: Bo Jacobsson MD, professor & Ronald Lamont MD, professor. • Advisor concerning diagnostic criteria: Jørgen Skov Jensen (Statens Seruminstitut) Objective The objective of this guideline is to evaluate bacterial vaginosis in pregnancy concerning • Different treatments for prevention of preterm delivery. • Screening of pregnant women with low as well as high risk of sPTD. • Stratification into gestational ages below and above 16 weeks. • Diagnostic methods. Key words Bacterial vaginosis, vaginal pH, pH-glove, vaginal discharge, Nugent score, Amsel score, Gardnerella vaginalis, Mobiluncus species, preterm delivery, preterm birth, GRADE, clindamycin, metronidazole, screening, treatment, probiotics, antibiotics, dysbiosis, abnormal vaginal flora. -
Recurrent Miscarriage
The Investigation and Treatment of Couples with Recurrent First- trimester and Second-trimester Miscarriage Green-top Guideline No. 17 April 2011 The Investigation and Treatment of Couples with Recurrent First-trimester and Second-trimester Miscarriage This is the third edition of this guideline , which was first published in 1998 and then in 2003 under the title The Investigation and Treatment of Couples with Recurrent Miscarriage . 1. Purpose and scope The purpose of this guideline is to provide guidance on the investigation and treatment of couples with three or more first -trimester miscarriages, or one or more second-trimester miscarriages . 2. Background and introduction Miscarriage is defined as the spontaneous loss of pregnancy before the fetus reaches viability. The term therefore includes all pregnancy losses from the time of conception until 24 weeks of gestation. It should be noted that advances in neonatal care have resulted in a small number of babies surviving birth before 24 weeks of gestation. Recurrent miscarriage, defined as the loss of three or more consecutive pregnancies, affects 1% of couples trying to conceive. 1 It has been estimated that 1–2% of second -trimester pregnancies miscarry before 24 weeks of gestation. 2 3. Identification and assessment of evidence The Cochrane Library and Cochrane Register of Controlled Trials were searched for relevant randomised controlled trials, systematic reviews and meta-analyses. A search of Medline from 1966 to 2010 was also carried out. The date of the last search was November 2010. In addition, relevant conference proceedings and abstracts were searched. The databases were searched using the relevant MeSH terms including all sub-headings. -
Vaginal Probiotics for Reproductive Health and Related Dysbiosis: Systematic Review and Meta-Analysis
Journal of Clinical Medicine Review Vaginal Probiotics for Reproductive Health and Related Dysbiosis: Systematic Review and Meta-Analysis Ana López-Moreno 1,2,* and Margarita Aguilera 1,2,3,* 1 Department of Microbiology, Faculty of Pharmacy, Campus of Cartuja, University of Granada, 18071 Granada, Spain 2 Instituto de Nutrición y Tecnología de los Alimentos, INYTA-Granada, 18100 Granada, Spain 3 Instituto de Investigación Biosanitaria, Ibs-Granada, 18012 Granada, Spain * Correspondence: [email protected] (A.L.-M.); [email protected] (M.A.); Tel.: +34-9-5824-5129 (M.A.); Fax: +34-958-246235 (M.A.) Abstract: The use of probiotics in reproductive-related dysbiosis is an area of continuous progress due to the growing interest from clinicians and patients suffering from recurrent reproductive microbiota disorders. An imbalance in the natural colonization sites related to reproductive health—vaginal, cervicovaginal, endometrial, and pregnancy-related altered microbiota—could play a decisive role in reproductive outcomes. Oral and vaginal administrations are in continuous discussion regarding the clinical effects pursued, but the oral route is used and studied more often despite the need for further transference to the colonization site. The aim of the present review was to retrieve the standard- ized protocols of vaginal probiotics commonly used for investigating their microbiota modulation capacities. Most of the studies selected focused on treating bacterial vaginosis (BV) as the most common dysbiosis; a few studies focused on vulvovaginal candidiasis (VVC) and on pretreatment during in vitro fertilization (IVF). Vaginal probiotic doses administered were similar to oral probiotics Citation: López-Moreno, A.; 7 10 Aguilera, M. Vaginal Probiotics for protocols, ranging from ≥10 CFU/day to 2.5 × 10 CFU/day, but were highly variable regarding Reproductive Health and Related the treatment duration timing. -
The Prevalence of Bacterial Vaginosis and Candidiasis in Postmenopausal Women
THE PREVALENCE OF BACTERIAL VAGINOSIS AND CANDIDIASIS IN POSTMENOPAUSAL WOMEN Ivanka Hadji Petrusheva Meloska1, Konstantin Icev1, Branko Jaglikovski1, Anica Hadji-Petrusheva Jankijevikj2, Biljana Curcic-Trajkovska2. 1Avicena Laboratory, Skopje, Macedonia.; 2Clinical Hospital Acibadem Sistina, Skopje, Macedonia Graph 3. Nugent‘s score among different age groups INTRODUCTION MATERIAL AND METHODS RESULTS The prevalence of BV (assessed as a Bacterial vaginosis (BV) is the most A total of 750 vaginal and cervical 500 NS of >or=7) in postmenopausal common cause of vaginal symptoms swabs, collected from 450 women was low (5,6% - 42 cases) and 400 characterized by a change of vaginal postmenopausal women age 40-69 350 NS1-4 GV was isolated in most of those 300 flora from years, were examined. BV was NS 4-6 cases. A high percentage (51.0% - 383) 250 predominant Lactobacillus to diagnosed microscopically by 200 NS>7 of postmenopausal women (NS of 1-4) 150 replacement with anaerobes such as Nugent's score (NS), the presence of had decreased number of lactobacilli 100 Gardnerella vaginalis (GV). Bacterial leukocytes in the slides was noted and no BV -associated 50 0 vaginosis occurs when the normal too. Detection of aerobic and microorganisms. In this category there age 40-49 age 50-59 age 60-69 lactobacilli of the vagina are replaced anaerobic bacteria and fungi was were 35 cases with leukocytes in the Graph 4. Microbiology findings by mostly anaerobic bacteria. performed by conventional vaginal smear, where cultures 60 p<0.05 Diagnosis is commonly made using microbiological methods. revealed presence of enterobacteriaceae and gram positive 50 the Nugent criteria, which include 40 cocci.