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The The 10 µg/mL. 10 For 10⁸ total cells, up to 100 tests. up100 to cells, For 10⁸ total Store protected from light at 2−8 light from Store protected mL DAPI Staining Solution: Solution: DAPI1 mL Staining vial label. vial freeze. The expiration date is indicated on the on indicated is date expiration The freeze. supplied in distilled water at a concentration of at a concentration water distilled in supplied 68, 5142968, Fax Fax +49 2204 85197 2204 +49 ready-to-use Bergisch Gladbach, Germany Bergisch Gladbach, DAPI Staining Solution is is Solution DAPI Staining nm and lower emission lower emission and nm nm. DAPI binds also also DAPI binds nm. nm DNA-bound nm °C. Do not Do °C. 10⁶ human peripheral blood mononuclear cells (PBMCs) old, ,8days cells mononuclear blood peripheral 10⁶ human Viable cells belong to the P2 population. P2 the to belong cells Viable 1.3 2. DAPI Staining Solution Miltenyi Biotec provides technical support worldwide. Visit worldwide. support technical provides Biotec Miltenyi was excluded from the analysis based on scatter signals. Dead Dead signals. on scatter based analysis the from excluded was analyzed directly and Solution DAPI Staining the with were stained www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec Biotec Miltenyi nearest your find to www.miltenyibiotec.com/local For dead cell exclusion add 10 µL of DAPI Staining Solution to 10⁶ to Solution of DAPI 10 add Staining µL exclusion cell For dead It is recommended to use DAPI Staining Solution at a final at afinal Solution DAPI use Staining to It recommended is Refer to to Refer cells are positive for DAPI and thus can be excluded from analysis. analysis. from excluded be can for DAPI thus and positive are cells 1 in cells investigator each vary, application Since of 0.1 µg/mL. concentration contact. should titrate the reagent to obtain optimal results. optimal obtain to reagent the titrate should by flow cytometry using the MACSQuant Analyzer 10. Cell debris debris Cell 10. Analyzer MACSQuant the using cytometry by flow page 1/2 Recommended dilution dilution Recommended Example of cell staining with the DAPI the with Staining staining of cell Example Solution

www.miltenyibiotec.com Side scatter analysis. cytometric flow to directly and proceed buffer mL 1 250 500 750 000 0 -1 P1/P2 0 1 10 D API ¹1 M 0² for all data sheets and protocols. protocols. and sheets data for all Order no. 130-111-570 10 ³

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