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SCYL1 Rabbit pAb

Catalog No. A6735 Category Polyclonal Antibodies Applications WB, IHC, IF Observed MW 110kDa Cross-reactivity Human, Mouse, Rat Calculated MW 69kDa/78kDa/86kDa/88kDa/89kDa

Immunogen Information Recommended Dilutions

Immunogen Recombinant fusion protein containing a sequence WB 1:500 - 1:2000 corresponding to amino acids 460-740 of human SCYL1 (NP_065731.3). IHC 1:50 - 1:200 ID 57410 IF 1:50 - 1:100 Swiss prot Q96KG9 Synonyms SCYL1;GKLP;HT019;NKTL;NTKL;P105;SCAR21;TAPK;TE IF;TRAP Product information

Source Rabbit Isotype IgG Purification method Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Western blot analysis of extracts of Background various cell lines, using SCYL1 Antibody (A6735) at 1:1000 dilution. This gene encodes a transcriptional regulator belonging to the SCY1-like family of Secondary antibody: HRP Goat Anti- kinase-like proteins. The protein has a divergent N-terminal kinase domain that is Rabbit IgG (H+L) (AS014) at 1:10000 thought to be catalytically inactive, and can bind specific DNA sequences through its dilution. C-terminal domain. It activates transcription of the telomerase reverse transcriptase Lysates/proteins: 25ug per lane. and DNA polymerase beta . The protein has been localized to the nucleus, and Blocking buffer: 3% nonfat dry milk in also to the cytoplasm and during . Multiple transcript variants TBST. encoding different isoforms have been found for this gene. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Immunohistochemistry of paraffin- embedded human liver damage using Immunohistochemistry of paraffin- Immunofluorescence analysis of MCF-7 SCYL1 antibody (A6735) at dilution of embedded rat kidney using SCYL1 cells using SCYL1 antibody (A6735). 1:100 (40x lens). antibody (A6735) at dilution of 1:100 Blue: DAPI for nuclear staining. (40x lens).

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