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Growth, Development, and Survival of Nosema Pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1ab B

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Growth, Development, and Survival of Nosema Pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1ab B Entomology Publications Entomology 2004 Growth, Development, and Survival of Nosema pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1Ab B. J. Reardon Iowa State University Richard L. Hellmich Iowa State University, [email protected] Douglas V. Sumerford United States Department of Agriculture Leslie C. Lewis Iowa State University Follow this and additional works at: http://lib.dr.iastate.edu/ent_pubs Part of the Entomology Commons, and the Plant Breeding and Genetics Commons The ompc lete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ ent_pubs/92. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the Entomology at Iowa State University Digital Repository. It has been accepted for inclusion in Entomology Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Growth, Development, and Survival of Nosema pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1Ab Abstract Transgenic corn, Zea mays L., hybrids expressing crystal protein endotoxin genes fromBacillus thuringiensis Berliner are an increasingly popular tactic for managing the European corn borer, Ostrinia nubilalis (Hübner), in North America. O. nubilalis populations also are often vulnerable to the ubiquitous entomopathogenic microsporidium Nosema pyrausta(Paillot). We examined the effect of feeding meridic diet incorporated with purified Cry1Ab on growth, development, and survival of Nosema-infected and uninfected neonate O. nubilalis. Infected larvae developed more slowly than uninfected larvae. Increasing the concentration of Cry1Ab in diet reduced larval development, and this effect was amplified by microsporidiosis. Infected larvae weighed significantly less than uninfected larvae. The er lationship among Nosema infection, Cry1Ab concentration, and larval weight was fitted to an exponential function. The CL 50 of infected larvae was one- third that of uninfected larvae, indicating that infected larvae are more vulnerable to toxin. This work has implications for resistance management of O. nubilalis and demonstrates that it is important to determine whether N. pyrausta is present when testing susceptibility of larvae to transgenic corn hybrids. Keywords Ostrinia nubilalis, Nosema pyrausta, Bt, corn Disciplines Entomology | Plant Breeding and Genetics Comments This article is from Journal of Economic Entomology; 97 (2004); 1198-1201; doi: 10.1603/ 0022-0493-97.4.1198 Rights Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The onc tent of this document is not copyrighted. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ent_pubs/92 Growth, Development, and Survival of Nosema pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1Ab Author(s): B. J. Reardon , R. L. Hellmich , D. V. Sumerford , and L. C. Lewis Source: Journal of Economic Entomology, 97(4):1198-1201. 2004. Published By: Entomological Society of America DOI: http://dx.doi.org/10.1603/0022-0493-97.4.1198 URL: http://www.bioone.org/doi/full/10.1603/0022-0493-97.4.1198 BioOne (www.bioone.org) is a nonprofit, online aggregation of core research in the biological, ecological, and environmental sciences. BioOne provides a sustainable online platform for over 170 journals and books published by nonprofit societies, associations, museums, institutions, and presses. Your use of this PDF, the BioOne Web site, and all posted and associated content indicates your acceptance of BioOne’s Terms of Use, available at www.bioone.org/page/terms_of_use. Usage of BioOne content is strictly limited to personal, educational, and non-commercial use. Commercial inquiries or rights and permissions requests should be directed to the individual publisher as copyright holder. BioOne sees sustainable scholarly publishing as an inherently collaborative enterprise connecting authors, nonprofit publishers, academic institutions, research libraries, and research funders in the common goal of maximizing access to critical research. BIOLOGICAL AND MICROBIAL CONTROL Growth, Development, and Survival of Nosema pyrausta-Infected European Corn Borers (Lepidoptera: Crambidae) Reared on Meridic Diet and Cry1Ab B. J. REARDON, R. L. HELLMICH, D. V. SUMERFORD, AND L. C. LEWIS Corn Insects and Crop Genetics Research Unit, USDAÐARS, Genetics Laboratory, Iowa State University, Ames, IA 50011 J. Econ. Entomol. 97(4): 1198Ð1201 (2004) ABSTRACT Transgenic corn, Zea mays L., hybrids expressing crystal protein endotoxin genes from Bacillus thuringiensis Berliner are an increasingly popular tactic for managing the European corn borer, Ostrinia nubilalis (Hu¨ bner), in North America. O. nubilalis populations also are often vulnerable to the ubiquitous entomopathogenic microsporidium Nosema pyrausta (Paillot). We examined the effect of feeding meridic diet incorporated with puriÞed Cry1Ab on growth, development, and survival of Nosema-infected and uninfected neonate O. nubilalis. Infected larvae developed more slowly than uninfected larvae. Increasing the concentration of Cry1Ab in diet reduced larval development, and this effect was ampliÞed by microsporidiosis. Infected larvae weighed signiÞcantly less than uninfected larvae. The relationship among Nosema infection, Cry1Ab concentration, and larval weight was Þtted to an exponential function. The LC50 of infected larvae was one-third that of uninfected larvae, indicating that infected larvae are more vulnerable to toxin. This work has implications for resistance management of O. nubilalis and demonstrates that it is important to determine whether N. pyrausta is present when testing susceptibility of larvae to transgenic corn hybrids. KEY WORDS Ostrinia nubilalis, Nosema pyrausta, Bt, corn THE EUROPEAN CORN BORER, Ostrinia nubilalis (Hu¨ b- Cry1Aa, Cry1Ab, Cry1Ac, and others (Rukmini et al. ner), is estimated to have entered the United States 2000, Huang et al. 2002) that may maximize the toxicity from Europe between 1909 and 1914 (Smith 1920, of this Bt preparation to O. nubilalis (Mohd-Salleh and Fracker and Fluke 1926). Since its arrival, O. nubilalis Lewis 1982). In contrast to insecticidal sprays for O. has established itself as a major pest of corn, Zea mays nubilalis, gene transfer technology currently uses one or L. (Cyperales: Poaceae). Rice (1994) suggested that a few genes, including the cry1Ab crystal protein endo- yield loss due to O. nubilalis may reach 81.5 bushels toxin gene (Pilcher et al. 2002). It is, therefore, important per hectare. Genetically modiÞed corn hybrids that to assess the interactions of O. nubilalis, N. pyrausta, and express crystal protein endotoxin genes from Bacillus Cry1Ab. The objective of this research was to examine thuringiensis Berliner (Bacillales: Bacillaceae) (Bt) for the effect of puriÞed Cry1Ab on growth, development, the control of O. nubilalis have been available com- and survival of Nosema-infected and uninfected O. nu- mercially since 1996 (Koziel et al. 1993) and are an bilalis. increasingly popular management tactic (Pilcher et al. Materials and Methods 2002). However, development of resistance by pestif- erous Lepidoptera may reduce the duration of efÞca- Insect and Pathogen Cultures. Adult O. nubilalis cious use of transgenic technology. collected during the summer of 2002 from light traps Nosema pyrausta (Paillot) (Microspora: Nosemati- were used to establish a colony at the Corn Insects and dae) is probably the most chronically detrimental, nat- Crop Genetics Research Unit, Ames, IA. To ensure urally occurring pathogen of O. nubilalis in the United that the colony was Nosema-free, eggs were heat- States (Lewis and Lynch 1978). A ubiquitous microspo- treated (Raun 1961). Moths were reared following ridium, N. pyrausta reduces egg hatch, developmental procedures similar to Guthrie et al. (1965). rate, fecundity, and life span of O. nubilalis (Zimmack N. pyrausta spores were isolated from Þeld-col- and Brindley 1957, Windels et al. 1976). Pierce et al. lected O. nubilalis in 2002. Infected larvae were ho- (2001) examined the interactions of Dipel ES (a spray mogenized in a glass tissue grinder with 10ϫ phos- formulation of Bt), N. pyrausta, and O. nubilalis, and they phate-buffered saline (PBS), and the homogenate was concluded that the susceptibility of O. nubilalis to Dipel Þltered through cheesecloth. Aureomycin (50 mg/ml ES increased when infected with N. pyrausta. Dipel ES, solution) was added to solution to inhibit microbial however, contains bacterial spores and several differ- growth. The concentration of spores was determined ent endotoxins active against Lepidoptera, including by using a hemocytometer (Levy, Horsham, PA) un- August 2004 REARDON ET AL.: GROWTH,DEVELOPMENT, AND SURVIVAL OF EUROPEAN CORN BORER 1199 der 400ϫ phase contrast microscopy. The suspension option of the SAS procedure PROC FREQ, SAS In- was frozen at Ϫ20ЊC when not in use. stitute 1988). Instar data were pooled over replications PuriÞed trypsin activated Cry1Ab (HPLC chromato- because visual inspection of the data failed to indicate gram-demonstrated purity) was obtained from Dr. M. a trend temporally. Carey (see Acknowledgments). The toxin was activated The inßuences of Nosema infection,
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