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The Interdigestive Motor Complex of Normal Subjects and Patients with Bacterial Overgrowth of the Small Intestine

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The Interdigestive Motor Complex of Normal Subjects and Patients with Bacterial Overgrowth of the Small Intestine The Interdigestive Motor Complex of Normal Subjects and Patients with Bacterial Overgrowth of the Small Intestine G. Vantrappen, … , J. Hellemans, Y. Ghoos J Clin Invest. 1977;59(6):1158-1166. https://doi.org/10.1172/JCI108740. Research Article Intraluminal pressures were measured in the gastric antrum and at different levels of the upper small intestine in 18 normal subjects to investigate whether or not the interdigestive motor complex, identified in several animal species, occurs in man and, if so, to determine its characteristics. In all normal subjects, the activity front of the interdigestive motor complex was readily identified as an uninterrupted burst of rhythmic contraction waves that progressed down the intestine and that was followed by a period of quiescence. Quantitative analysis of various parameters of the complex and simultaneous radiological and manometrical observations revealed that it resembled closely the canine interdigestive motor complex. To test the hypothesis that disorders of this motor complex may lead to bacterial overgrowth in the small 14 intestine, similar studies were performed in 18 patients with a positive CO2 bile acid breath test and in an additional 14 control group of 9 patients with a normal CO2 breath test. All but five patients had normal interdigestive motor complexes. The five patients in whom the motor complex was absent or greatly disordered had bacterial overgrowth as 14 evidenced by CO2 bile acid breath tests before and after antibiotics. These studies establish the presence and define the characteristics of the normal interdigestive motor complex in man. They also suggest that bacterial overgrowth may be due to a […] Find the latest version: https://jci.me/108740/pdf The Interdigestive Motor Complex of Normal Subjects and Patients with Bacterial Overgrowth of the Small Intestine G. VANTRAPPEN, J. JANSSENS, J. HELLEMANS, and Y. GHOOS From the Department of Medical Research, University of Leuven, and the Department of Medicine, Akademisch Ziekenhuis St. Rafael, B-3000 Leuven, Belgium A B S T R A C T Intraluminal pressures were measured in the duodenum. Feeding interrupted this cycle and in the gastric antrum and at different levels of the changed the tracing into a pattern of irregular spiking upper small intestine in 18 normal subjects to investi- activity. This observation has been confirmed and ex- gate whether or not the interdigestive motor complex, tended by other investigators who have called the identified in several animal species, occurs in man and, phenomenon the "interdigestive myoelectric com- if so, to determine its characteristics. In all normal plex" (2). An analogous cyclically recurring sequence subjects, the activity front of the interdigestive motor of regular spiking activity has been observed in several complex was readily identified as an uninterrupted animal species, including sheep (3), calves (4), horses burst of rhythmic contraction waves that progressed (5), and rats (6). Very little is known about the pres- down the intestine and that was followed by a period of ence of this complex in man and nothing about its quiescence. Quantitative analysis of various param- characteristics and function. Kyfareva (7) and Foulk eters of the complex and simultaneous radiological et al. (8) studied the motility of the human upper and manometrical observations revealed that it re- small intestine. They described periods of activity sembled closely the canine interdigestive motor alternating with periods of quiescence and recognized complex. To test the hypothesis that disorders of this that sometimes this motility pattern developed into motor complex may lead to bacterial overgrowth in a burst of rhythmic contractions of several minutes the small intestine, similar studies were performed in duration terminating the active phase. It has been 18 patients with a positive 14CO2 bile acid breath shown that these bursts progress aborally in the duo- test and in an additional control group of 9 patients denum of man (9). This rhythmic sequence probably with a normal 14CO2 breath test. All but five pa- corresponds to the activity front (2) of the interdiges- tients had normal interdigestive motor complexes. tive myoelectric complex. The cyclic recurrence of the The five patients in whom the motor complex was phenomenon and its migration down the intestine were absent or greatly disordered had bacterial overgrowth not recognized. On the basis of simultaneous radio- as evidenced by 14CO2 bile acid breath tests before logical and electromyographical observations, it has and after antibiotics. These studies establish the been stated that this activity front serves to clean up presence and define the characteristics of the normal the remnants of the preceding meal and functions as interdigestive motor complex in man. They also suggest the "house keeper" of the small intestine (10). that bacterial overgrowth may be due to a specific The first purpose of the present study was to investi- motility disorder i.e., complete or almost complete gate whether or not an interdigestive motor complex absence of the interdigestive motor complex. occurs in humans and, if so, to determine its charac- teristics. If this interdigestive complex functions as a INTRODUCTION house keeper, it seems natural to speculate that a deficient clearing mechanism may result in accumula- In 1969, Szurszewski (1) identified in the small in- tion of food remnants, desquamated cells, and secre- testine of fasted dogs an electric complex charac- tions, thus creating a medium favorable to bacterial terized by a front of intense spiking activity that overgrowth in the small intestine. Therefore, the migrated down the entire small bowel. As the complex second purpose of this study was to test the hypothesis reached the ileum, another activity front developed that abnormalities of the interdigestive motor complex 1158 The Journal of Clinical Investigation Volume 59 June 1977* 1158 -1166 may lead to bacterial colonization of the small intes- at the level of the angle of Treitz; (b) aboral progression tine. of the activity front, as indicated by its sequential passage over all three or over the distal two recording orifices; (c) a period of complete quiescence after the activity front. METHODS If a burst of rhythmic contractions was observed at the Normal subjects and patients. 18 normal subjects, without level of the distal recording orifice only, and if this burst was gastrointestinal symptoms or signs and with a normal followed by a period of complete quiescence, this pattern 14CO2 bile acid breath test, were studied. Their ages ranged could not with certainty be interpreted as an activity front from 18 to 71 yr, with a mean of 33 yr. There were 11 men because there was no proof of its aboral progression. As and 7 women. 18 patients, selected on the basis of an this complex of pressure changes is similar to the pattern pro- abnormal 14CO2 bile acid breath test, were also investigated. duced by an activity front, it is logical to assume that it is After the motility study, they were treated for 5 days with produced by an activity front that starts between the two distal a daily dose of 2 g of Tetrex (tetracylcine phosphate com- recording orifices. Such pressure complexes were called plex) (Bristol Laboratories, Syracuse, N.Y.) by mouth. A "probable interdigestive motor complexes". The activity second "4CO2 bile acid breath test was then performed fronts were identified independently by two of the authors. to distinguish between ileal malabsorption and small intes- The tracings were coded so that no clinical information tinal bacterial overgrowth (11, 12). Nine patients with various was available to the analyzers. Up to this phase of the diseases of the digestive system but with a negative 14CO2 analysis, interobserver variation was limited to only one breath test were studied as an additional control group. activity front. Some pertinent data on the patients are summarized in The final step of the analysis consisted of determining Table I. the duration of the bursts of rhythmic contractions at dif- Motility recordings. Intraluminal pressures were meas- ferent levels (duration of activity fronts), the frequency of ured by means of three polyethylene catheters (PE 205, the contraction waves during the periods of rhythmic ac- Clay Adams, Div. of Becton, Dickinson and Co., Parsippany, tivity, the progression velocity of the activity front, the N. J.) with side openings 25 cm apart. The catheters calculated length of the activity front (duration x progres- were perfused continuously at a rate of 0.19 ml/min (Har- sion velocity), the interval between meal and appearance vard pump, Harvard Apparatus Co., Inc., Millis, Mass.). of the first activity front, and the duration of the complete External pressure transducers (EMT 34, Elema Schonander, cycle of the motor complex. As this phase of the analysis Stockholm, Sweden) were used and the tracings were re- is subject to observer bias and requires the drawing of marks corded on a polygraph (Mingograph 81, Elema Schonander). on the tracings, it was carried out jointly by three authors. The catheter assembly included two other tiny polyethylene The data were analyzed statistically by means of Student's tubes (PE 60, Clay Adams) which ended in a mercury- t test (13). filled latex bag 15 cm below the most aboral recording 14C02 Bile acid breath test. The bile acid breath test orifice. The mercury-filled bag facilitated the passage of the was performed according to the method of Fromm et al. catheter system through the small intestine whereas the two (14) with slight modifications. After an overnight fast, subjects additional catheters made it possible to aspirate the mercury. and patients took with their breakfast a starch capsule In three subjects of the control group, the most proximal containing 5 ACi cholyl-['4C glycine] (The Radiochemi- recording site was temporarily located in the antrum. In cal Centre, Amersham, Buckinghamshire, England). all other normal subjects and in the patients, the proximal Breath samples were collected before and 1, 2, 3, 4, 5, 6.
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