SPECIFICATION SHEET Rev 110519MAB-J1585

Mouse Anti-Human DSC3 Monoclonal Antibody Mouse, Monoclonal (DSC3) Cat. No. DMAB1585MH Lot. No. (See product label)

PRODUCT INFORMATION ANTIGEN INFORMATION

Product Overview: MAb to 3 Gene Name: DSC3 desmocollin 3 [ Homo sapiens ] Monoclonal Antibody to Human Desmocollin 3 Official Symbol: DSC3 Antigen Description: Desmocollin-3 is a that in hu- Synonyms: DSC; DSC1; DSC2; DSC4; CDHF3; HT-CP; mans is encoded by the DSC3 gene. DSC3; desmocollin 3; desmocollin-3; desmocollin-4; cad- Immunogen: Synthetic peptide corresponding to a se- herin family member 3; Desmocollin-4 quence present on the extracellular anchor domain of human GeneID: 1825 desmocollin 3 (VHGAPFYFSLPNTSPEISRLWSLTKVC) mRNA Refseq: NM_001941 Isotype: IgG1 Protein Refseq: NP_001932 Specificity: MAb localizes Dsc3 present in all living epider- MIM: 600271 mal layers, in glandular duct cells, in basal matrix cells and UniProt ID: Q14574 outer root sheath of hair follicles, basal and suprabasal cell Location: 18q12.1 layers of stratified epithelia (e.g. vagina, tongue, esophagus), Function: calcium ion binding; gamma-catenin bindin basal layer of bladder urothelium and complex epithelium of trachea, and thymic reticulum cells. All simple epithelia are PACKAGING negative. Intercalated disks of myocardium are negative. Polypeptide reacting: 109kDa and 10 kDa polypeptides Concentration: Not determined (desmocollin 3 splice isoforms at pI 5.2) from human epider- Buffer: Not applicable mal . Most cultured cell lines derived from Storage: Store at 2–8°C stratified squamous epithelia or squamous cell carcinomas Warning: This product contains sodium azide, which has are positive (e.g. A-431, HaCaT). Specific for human des- been classified as Xn (Harmful), in European Directive mocollin 3. Does not react with bovine and rodent. 67/548/EEC in the concentration range of 0.1–1.0%. When Clone: Esc4-U115 disposing of this reagent through lead or copper plumbing, Format: Supernatant, Liquid flush with copious volumes of water to prevent azide build-up Host animal: Mouse in drains. Source: Cell culture Application: Suitable for Immunohistochemistry on frozen REFERENCES and paraffin-embedded sections after microwave treatment 1. Kurzen, H., et al., (1998), “Compositionally different des- (use undiluted) and Western blot. For better resolution on mosomes in the various compartments of the frozen sections preincubation (directly after fixation) with human hair follicle”, Differentiation, 63, 295-304. 0.05 – 0.2% Triton X100, for 5-10 minutes depending on 2. South, A.P., et al., (2003), “Lack of plakophilin 1 increases tissue type, is recommended. Each laboratory should deter- keratinocyte migration and reduces stability”, J. mine an optimum working titer for use in its particular appli- Cell Sci., 116, 3303-3314. cation. Other applications have not been tested but use in such assays should not necessarily be excluded. Purification: Not applicable Preservative: 0.09% Sodium azide Affinity Constant: Not determined

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