CPI-17 Antibody / PPP1R14A (R32782)

Catalog No. Formulation Size

R32782 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug Bulk quote request

Availability 1-3 business days

Species Reactivity Human, Mouse, Rat

Format Antigen affinity purified

Clonality Polyclonal (rabbit origin)

Isotype Rabbit IgG

Purity Antigen affinity

Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

UniProt Q96A00

Localization Cytoplasmic, nuclear

Applications Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 1-2ug/ml Flow cytometry : 1-3ug/10^6 cells Immunofluorescence : 2-4ug/ml

Limitations This CPI-17 antibody is available for research use only.

IHC testing of FFPE human lung cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing. IHC testing of FFPE human intestinal cancer tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE human placental tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

IHC testing of FFPE mouse testis tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing. IHC testing of FFPE rat lung tissue with CPI-17 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Western blot testing of 1) rat brain, 2) mouse brain and 3) human PANC1 lysate with CPI-17 antibody at 0.5ug/ml. Predicted molecular weight ~17 kDa.

Flow cytometry testing of human U-2 OS cells with CPI-17 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CPI-17 antibody. IHC testing of FFPE human U-2 OS cells with CPI-17 antibody (green) and DAPI (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.

Description Protein phosphatase 1 regulatory subunit 14A, also known as CPI-17, is a protein that in humans is encoded by the PPP1R14A gene. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Application Notes Optimal dilution of the CPI-17 antibody should be determined by the researcher.

Immunogen Amino acids L30-Q126 from human CPI-17 alpha were used as the immunogen for the CPI-17 antibody.

Storage After reconstitution, the CPI-17 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.