Comparison of Mechanical Property and Role Between Enamel And
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Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate
materials Article Preparation of Absorption-Resistant Hard Tissue Using Dental Pulp-Derived Cells and Honeycomb Tricalcium Phosphate Kiyofumi Takabatake 1, Keisuke Nakano 1,* , Hotaka Kawai 1, Yasunori Inada 1, Shintaro Sukegawa 1,2 , Shan Qiusheng 1, Shigeko Fushimi 1, Hidetsugu Tsujigiwa 1,3 and Hitoshi Nagatsuka 1 1 Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama 700-8525, Japan; [email protected] (K.T.); [email protected] (H.K.); [email protected] (Y.I.); [email protected] (S.S.); [email protected] (S.Q.); [email protected] (S.F.); [email protected] (H.T.); [email protected] (H.N.) 2 Department of Oral and Maxillofacial Surgery, Kagawa Prefectural Central Hospital, Kagawa 760-8557, Japan 3 Department of Life Science, Faculty of Science, Okayama University of Science, Okayama 700-0005, Japan * Correspondence: [email protected] Abstract: In recent years, there has been increasing interest in the treatment of bone defects using undifferentiated mesenchymal stem cells (MSCs) in vivo. Recently, dental pulp has been proposed as a promising source of pluripotent mesenchymal stem cells (MSCs), which can be used in various clinical applications. Dentin is the hard tissue that makes up teeth, and has the same composition and strength as bone. However, unlike bone, dentin is usually not remodeled under physiological conditions. Here, we generated odontoblast-like cells from mouse dental pulp stem cells and combined them with honeycomb tricalcium phosphate (TCP) with a 300 µm hole to create bone-like Citation: Takabatake, K.; Nakano, K.; tissue under the skin of mice. -
Collagen Fibres Are Not Required for Initial Matrix Mineralization by Bone Cells
Cells and Materials Volume 6 Number 1 Numbers 1-3 Article 23 1996 Collagen Fibres are Not Required for Initial Matrix Mineralization by Bone Cells M. M. Hosseini University of Toronto S. A. F. Peel University of Toronto J. E. Davies University of Toronto Follow this and additional works at: https://digitalcommons.usu.edu/cellsandmaterials Part of the Biomedical Engineering and Bioengineering Commons Recommended Citation Hosseini, M. M.; Peel, S. A. F.; and Davies, J. E. (1996) "Collagen Fibres are Not Required for Initial Matrix Mineralization by Bone Cells," Cells and Materials: Vol. 6 : No. 1 , Article 23. Available at: https://digitalcommons.usu.edu/cellsandmaterials/vol6/iss1/23 This Article is brought to you for free and open access by the Western Dairy Center at DigitalCommons@USU. It has been accepted for inclusion in Cells and Materials by an authorized administrator of DigitalCommons@USU. For more information, please contact [email protected]. Cells and Materials Vol. 6, No. 1-3, 1996 (Pages 233-250) 1051-6794/96$5.00+ .25 Scanning Microscopy International, Chicago (AMF O'Hare), IL 60666 USA COLLAGEN FIBRES ARE NOT REQUIRED FOR INITIAL MATRIX MINERALIZATION BY BONE CELLS M.M. Hosseini, S.A.F. Peel and J.E. Davies• Centre for Biomaterials, University of Toronto, 170 College Street, Toronto, Ontario, Canada, M5S 3E3 (Received for publication June 25, 1996 and in revised form December 27, 1996) Abstract Introduction Passaged primary cultures of young adult rat bone We have recently shown that differentiating osteo marrow cells were maintained in medium containing genic cell s, derived from explants of young adult rat combinations of the supplements dexamethasone, ascor bone marrow, elaborate an interfacial matrix with the bic acid and Na-{3-glycerophosphate. -
The Role of Bone Marrow-Derived Cells During Ectopic Bone
Int. J. Med. Sci. 2018, Vol. 15 748 Ivyspring International Publisher International Journal of Medical Sciences 2018; 15(8): 748- 757. doi: 10.7150/ijms.24605 Research Paper The Role of Bone Marrow-Derived Cells during Ectopic Bone Formation of Mouse Femoral Muscle in GFP Mouse Bone Marrow Transplantation Model Kiyofumi Takabatake1, Hidetsugu Tsujigiwa2, Yu Song1, Hiroyuki Matsuda1, Hotaka Kawai1, Masae Fujii1, Mei Hamada1, Keisuke Nakano1, Toshiyuki Kawakami3, Hitoshi Nagatsuka1 1. Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Science, Okayama University, Okayama, Japan; 2. Department of life science, Faculty of Science, Okayama University of Science, Okayama, Japan; 3. Hard Tissue Pathology Unit, Matsumoto Dental University Graduate School of Oral Medicine, Shiojiri, Japan. Corresponding author: Kiyofumi Takabatake, Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-Cho, Okayama 700-8558, Japan. Phone: (+81) 86-2351-6651; Fax: (+81) 86-235-6654; E-mail: [email protected] ama-u.ac.jp © Ivyspring International Publisher. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions. Received: 2017.12.27; Accepted: 2018.04.12; Published: 2018.05.22 Abstract Multipotential ability of bone marrow-derived cells has been clarified, and their involvement in repair and maintenance of various tissues has been reported. However, the role of bone marrow-derived cells in osteogenesis remains unknown. In the present study, bone marrow-derived cells during ectopic bone formation of mouse femoral muscle were traced using a GFP bone marrow transplantation model. -
Pulp Canal Obliteration After Traumatic Injuries in Permanent Teeth – Scientific Fact Or Fiction?
CRITICAL REVIEW Endodontic Therapy Pulp canal obliteration after traumatic injuries in permanent teeth – scientific fact or fiction? Juliana Vilela BASTOS(a) Abstract: Pulp canal obliteration (PCO) is a frequent finding associated (b) Maria Ilma de Souza CÔRTES with pulpal revascularization after luxation injuries of young permanent teeth. The underlying mechanisms of PCO are still unclear, (a) Universidade Federal de Minas Gerais - and no experimental scientific evidence is available, except the results UFMG, School of Dentistry, Department of Restorative Dentistry, Belo Horizonte, MG, of a single histopathological study. The lack of sound knowledge Brazil. concerning this process gives rise to controversies, including the (b) Pontifícia Universidade Católica de Minas most suitable denomination. More than a mere semantic question, Gerais – PUC-MG, Department of Dentistry, the denomination is an important issue, because it reflects the nature Belo Horizonte, MG, Brazil. of this process, and directly impacts the treatment plan decision. The hypothesis that accelerated dentin deposition is related to the loss of neural control over odontoblastic secretory activity is well accepted, but demands further supportive studies. PCO is seen radiographically as a rapid narrowing of pulp canal space, whereas common clinical features are yellow crown discoloration and a lower or non-response to sensibility tests. Late development of pulp necrosis and periapical disease are rare complications after PCO, rendering prophylactic endodontic intervention -
Sensitive Teeth Sensitive Teeth Can Be Treated
FOR THE DENTAL PATIENT ... TREATMENT Sensitive teeth Sensitive teeth can be treated. Depending on the cause, your dentist may suggest that you try Causes and treatment desensitizing toothpaste, which contains com- pounds that help block sensation traveling from the tooth surface to the nerve. Desensitizing f a taste of ice cream or a sip of coffee is toothpaste usually requires several applications sometimes painful or if brushing or flossing before the sensitivity is reduced. When choosing makes you wince occasionally, you may toothpaste or any other dental care products, look have a common problem called “sensitive for those that display the American Dental Asso- teeth.” Some of the causes include tooth ciation’s Seal of Acceptance—your assurance that Idecay, a cracked tooth, worn tooth enamel, worn products have met ADA criteria for safety and fillings and tooth roots that are exposed as a effectiveness. result of aggressive tooth brushing, gum recession If the desensitizing toothpaste does not ease and periodontal (gum) disease. your discomfort, your dentist may suggest in- office treatments. A fluoride gel or special desen- SYMPTOMS OF SENSITIVE TEETH sitizing agents may be applied to the sensitive A layer of enamel, the strongest substance in the areas of the affected teeth. When these measures body, protects the crowns of healthy teeth. A layer do not correct the problem, your dentist may rec- called cementum protects the tooth root under the ommend other treatments, such as a filling, a gum line. Underneath the enamel and the crown, an inlay or bonding to correct a flaw or cementum is dentin, a part of the tooth that is decay that results in sensitivity. -
Original Article Concurrent Measurements of Danger Zone Anatomy in Mandibular First Molars Using Micro-Computed Tomography: a Pilot Study
Int J Clin Exp Med 2018;11(8):7692-7700 www.ijcem.com /ISSN:1940-5901/IJCEM0067321 Original Article Concurrent measurements of danger zone anatomy in mandibular first molars using micro-computed tomography: a pilot study Jiawei Lu1, Lizong Liang1, Jie Ran1, Gao Wu1, Chenghao Li2, Jianping Ge2, Jianxiang Tao1 Departments of 1Prosthodontics, 2Endodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 20072, China Received October 16, 2017; Accepted May 9, 2018; Epub August 15, 2018; Published August 30, 2018 Abstract: The aim of this study is to describe the anatomical characteristics of the ‘danger zone’ in human mandibu- lar first molars by measuring the thickness of the bending point (point α) and the thinnest point (point β) of each root in human mandibular first molars. Eighteen mandibular first molars were scanned by micro-computed tomography and reconstructed three-dimensionally by Mimics Research 17.0. To identify characteristics of the ‘danger zone’, the actual root curvature, the minimal dentin thickness of the bending slice and the thinnest slice were selected as parameters. Furthermore, to describe the relationship of point α and point β, the distance between them was also measured. The results showed that the actual canal curvatures in the mandibular first molars were 5-10 degrees larger than those measured in 2-D images including X-ray or CBCT. The thinnest thickness of roots in all canals was less than 1 mm except the two distal roots of three-rooted mandibular first molars. Moreover, as for some two-rooted mandibular first molars, their point α and point β were located in the same furcation surface of root, the result might be coincident in the mesiolingual side; whereas for three-rooted mandibular first molars, the distal two canals were found significantly thicker than the mesial two (p<0.05). -
Anatomical Analysis of the Resected Roots of Mandibular First Molars After Failed Non-Surgical Retreatment
Restor Dent Endod. 2018 May;43(2):e16 https://doi.org/10.5395/rde.2018.43.e16 pISSN 2234-7658·eISSN 2234-7666 Research Article Anatomical analysis of the resected roots of mandibular first molars after failed non-surgical retreatment Jiyoung Yoon ,1 Byeong-Hoon Cho ,2 Jihyun Bae ,1 Yonghoon Choi 1* 1Department of Conservative Dentistry, Section of Dentistry, Seoul National University Bundang Hospital, Seongnam, Korea 2Department of Conservative Dentistry, Seoul National University School of Dentistry and Dental Research Institute, Seoul, Korea Received: Nov 29, 2017 ABSTRACT Accepted: Jan 26, 2018 Objectives: Yoon J, Cho BH, Bae J, Choi Y Understanding the reason for an unsuccessful non-surgical endodontic treatment outcome, as well as the complex anatomy of the root canal system, is very *Correspondence to important. This study examined the cross-sectional root canal structure of mandibular first Yong-Hoon Choi, DDS, MSD, PhD molars confirmed to have failed non-surgical root canal treatment using digital images Associate Professor, Department of Conservative Dentistry, Section of Dentistry, obtained during intentional replantation surgery, as well as the causative factors of the failed Seoul National University Bundang Hospital, conventional endodontic treatments. 82 Gumi-ro 173-beon-gil, Bundang-gu, Materials and Methods: This study evaluated 115 mandibular first molars. Digital Seongnam 13620, Korea. photographic images of the resected surface were taken at the apical 3 mm level and E-mail: [email protected] examined. The discolored dentin area around the root canal was investigated by measuring Copyright © 2018. The Korean Academy of the total surface area, the treated areas as determined by the endodontic filling material, and Conservative Dentistry the discolored dentin area. -
RANKL/OPG Ratio Regulates Odontoclastogenesis in Damaged
www.nature.com/scientificreports OPEN RANKL/OPG ratio regulates odontoclastogenesis in damaged dental pulp Daisuke Nishida1, Atsushi Arai2, Lijuan Zhao3, Mengyu Yang3, Yuko Nakamichi3, Kanji Horibe4, Akihiro Hosoya5, Yasuhiro Kobayashi3, Nobuyuki Udagawa6* & Toshihide Mizoguchi1,6* Bone-resorbing osteoclasts are regulated by the relative ratio of the diferentiation factor, receptor activator NF-kappa B ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). Dental tissue- localized-resorbing cells called odontoclasts have regulatory factors considered as identical to those of osteoclasts; however, it is still unclear whether the RANKL/OPG ratio is a key factor for odontoclast regulation in dental pulp. Here, we showed that odontoclast regulators, macrophage colony-stimulating factor-1, RANKL, and OPG were detectable in mouse pulp of molars, but OPG was dominantly expressed. High OPG expression was expected to have a negative regulatory efect on odontoclastogenesis; however, odontoclasts were not detected in the dental pulp of OPG-defcient (KO) mice. In contrast, damage induced odontoclast-like cells were seen in wild-type pulp tissues, with their number signifcantly increased in OPG-KO mice. Relative ratio of RANKL/OPG in the damaged pulp was signifcantly higher than in undamaged control pulp. Pulp damages enhanced hypoxia inducible factor-1α and -2α, reported to increase RANKL or decrease OPG. These results reveal that the relative ratio of RANKL/OPG is signifcant to pulpal odontoclastogenesis, and that OPG expression is not required for maintenance of pulp homeostasis, but protects pulp from odontoclastogenesis caused by damages. Osteoclasts are monocyte/macrophage-derived multinucleated bone-resorbing cells 1–3. Bone tissue is continu- ously resorbed by osteoclasts, and is subsequently replenished by osteoblasts4. -
Comparative Morphology of Incisor Enamel and Dentin in Humans and Fat Dormice (Glis Glis)
Coll. Antropol. 27 (2003) 1: 373–380 UDC 572.72:616.314.11 Original scientific paper Comparative Morphology of Incisor Enamel and Dentin in Humans and Fat Dormice (Glis glis) Dean Konjevi}1, Tomislav Keros2, Hrvoje Brki}3, Alen Slavica1, Zdravko Janicki1 and Josip Margaleti}4 1 Chair for Game Biology, Pathology and Breeding, Veterinary Faculty, University of Zagreb, Zagreb, Croatia 2 Croatian Veterinary Institute, Zagreb, Croatia 3 Department for Dental Anthropology, School of Dental Medicine, University of Zagreb, Zagreb, Croatia 4 Department of Forest Protection and Wildlife Management, Faculty of Forestry, University of Zagreb, Zagreb, Croatia ABSTRACT The structure of teeth in all living beings is genetically predetermined, although it can change under external physiological and pathological factors. The author’s hypoth- esis was to indicate evolutional shifts resulting from genetic, functional and other dif- ferences. A comparative study about certain characteristics of incisors in humans and myomorpha, the fat dormouse (Glis glis) being their representative as well, comprised measurements of enamel and dentin thickness in individual incisor segments, evalua- tion of external enamel index, and also assessment of histological structure of enamel and dentin. The study results involving dormice showed the enamel to be thicker in lower than in the upper teeth, quite contrary to enamel thickness in humans. In the up- per incisors in dormice the enamel is the thickest in the medial layer of the crown, and in the cervical portion of the crown in the lower incisors. The thickness of dentin in dor- mice is greater in the oral than in the vestibular side. These findings significantly differ from those reported in reference literature, but they are based on the function of teeth in dormice. -
Bonding to Enamel and Dentin
Chapter 16 Bonding to Enamel and Dentin INTRODUCTION • Evolution of Dentin Bonding Agents ADHESIVE DENTISTRY • Nanofilled Bonding Agents • Indications for Use of Adhesives HYBRID LAYER AND HYBRIDIZATION • Advantages of Bonding Techniques • Hybridization (Given by Nakabayachi in 1982) • Mechanism of Adhesion • Zones of Hybrid Layer • Factors Affecting Adhesion SMEAR LAYER ENAMEL BONDING • Structure • Steps for Enamel Bonding • Formation of Smear Layer • Mechanism of Etching • Components of Smear Layer DENTIN BONDING • Role of Smear Layer • Conditioning of Dentin • Role of Smear Layer in Dentin Bonding • Priming of Dentin • Classification of Modern Adhesives • Moist vs Dry Dentin GLASS IONOMER BASED ADHESIVE SYSTEM DENTIN BONDING AGENT • Steps • Mechanism of Bonding FAILURE OF DENTIN BONDING INTRODUCTION fluid present in dentinal tubules makes the bonding difficult. The adhesion to dentin requires decalcification The traditional “drill and fill” approach is fading now of the dentinal surface to expose a layer of interlacing because of numerous advancements taking place in collagen fibrils and the entrances of the dentinal tubules. restorative dentistry. For a restorative material, adhesion When resins are used, they form an intermediate layer is the primary requirement so that restorative materials with exposed spongy collagen network which can be then can be bonded to enamel or dentin and without the need bonded to the retentive inner surface of the restoration of extensive tooth preparation. The initial advancement by means of a resin similar to that of enamel bonding. was made in 1956, by a pedodontist, Buonocore, who The past decade has seen increased use of bonding developed acid etching of the enamel. He showed that agents in concurrence with traditional dental materials. -
The Microhardness of Enamel and Dentin R
THE MICROHARDNESS OF ENAMEL AND DENTIN R. G. CRAIG, PH.D., AND F. A. PEYTON, D.Sc. University of Michigan, School of Dentistry, Ann Arbor, Mich. THE hardness of enamel and dentin has been determined by a variety of methods including abrasion," 2 pendulum,' scratch,4-7 and indentation" teehnics. Since the hardness of enamel and dentin has been shown to have con- siderable local variations, the methods using a microscratch or microindentation have been preferred. One of the more common types is the Knoop diamond in- denter14 which has been used by a number of investigators.', 12, 15, 16 It should be mentioned, however, that in spite of the fact that the indentations are ex- tremely small, they still represent a macroindentation when compared to the microstructure of enamel and dentin. The majority of the published hardness data for enamel and dentin has been measured on ground sections, although several papers'0 13 reported the hardness of intact enamel surfaces. The conclusions in regard to the difference in hardness from one section of a tooth to another are at times in variance with each other. This study of dentin and enamel was undertaken in an attempt to establish any trends in hardness existing from one area of a tooth to another or between different types of teeth. With this purpose in mind, this research did not attempt to relate the hardness values to the histologic tooth structure, but a sufficiently large number of hardness measurements were made so that the data could be treated on a statistical basis. EXPERIMENTAL Specimen Preparation.-Mature, freshly extracted, noncarious teeth were imbedded in Ward's Bio-Plastic by suspending them in a Vaughn ring contain- ing the polymer mixed with the catalyst and accelerator. -
Microstructure of Primary Tooth Dentin
Scientific Article Microstructure of primary tooth dentin David A. Sumikawa, DDS, MS Grayson W. Marshall, DDS, MPH, PhD Lauren Gee, MPH Sally J. Marshall, PhD Dr. Sumikawa is in private pediatric dental practice in Honolulu, Hawaii. Dr. G. Marshall is Professor and Chair, Division of Biomaterials and Bioengineering, Ms. Gee is with the Department of Epidemiology and Biostatistics, and Dr. Sally Marshall is Professor and Vice-Chair for Research, Department of Restorative Dentistry, University of California, San Francisco, California. Abstract Purpose: This study was performed to determine variations in Restoration of primary teeth, particularly anterior teeth, is dentin microstructure from primary anterior teeth at specific ar- often difficult because of their small size, thinness of enamel, eas and depths in relation to the dentin enamal junction, (DEJ). enamel morphology, pulpal anatomy, and rapid spread and Methods: Ten freshly extracted, non-carious primary maxil- extent of decay.4 lary anterior teeth were sectioned to provide two 1.0 mm x 1.0 Dentin bond strength comparisons between primary and mm matchsticks extending from the DEJ to the pulp chamber— permanent teeth have shown mixed results. Salama and Tao5 one each from the central and distal regions of each tooth. Slices found lower bond strength to primary dentin, Bordin-Aykroyd were prepared at distances of 0.15, 0.8, and 1.45 mm from the et al.1 found higher bond strengths, while others.6,7 found no DEJ. Following polishing, each slice was examined in a wet scan- significant differences. ning election microscope, (SEM) and tubule density, tubular Tubule diameters and tubule numerical density increase diameter, and peritubular width were determined at nine grid from the dentinoenamel junction (DEJ), towards the pulp, with locations.