DOCSLIB.ORG

The Values of AHCY and CBS Promoter Methylation on the Diagnosis of Cerebral Infarction in Chinese Han Population

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Values of AHCY and CBS Promoter Methylation on the Diagnosis of Cerebral Infarction in Chinese Han Population Li et al. BMC Medical Genomics (2020) 13:163 https://doi.org/10.1186/s12920-020-00798-7 RESEARCH ARTICLE Open Access The values of AHCY and CBS promoter methylation on the diagnosis of cerebral infarction in Chinese Han population Xiaodong Li1, Shufang Bu1, Ran Ran Pan2, Cong Zhou2, Kun Qu3, Xiuru Ying2, Jie Zhong2, Jianhao Xiao4, Qian Yuan4, Simiao Zhang4, Laura Tipton5, Yunliang Wang4*, Youping Deng5* and Shiwei Duan2* Abstract Background: The goal of our study is to investigate whether the methylation levels of AHCY and CBS promoters are related to the risk of cerebral infarction by detecting the methylation level of AHCY and CBS genes. Methods: We extracted peripheral venous blood from 152 patients with cerebral infarction and 152 gender- and age-matched healthy controls, and determined methylation levels of AHCY and CBS promoters using quantitative methylation-specific polymerase chain reaction. We used the percentage of methylation reference (PMR) to indicate gene methylation level. Results: We compared the promoter methylation levels of two genes (AHCY and CBS) in peripheral blood DNA between the cerebral infarction case group and the control group. Our study showed no significant difference in AHCY promoter methylation between case and control. Subgroup analysis by gender showed that the methylation level of AHCY in males in the case group was lower than that in the control group, but the difference was not statistically significant in females. In a subgroup analysis by age, there was no significant difference in the AHCY methylation level between the case and control in the young group (≤44 years old). However, the level of AHCY gene methylation in the middle-aged group (45–59 years old) was significantly higher and the aged group (≥60 years old) was significantly lower than that in the control groups. However, CBS promoter methylation levels were significantly lower in the case group than in the control group (median PMR: 70.20% vs 104.10%, P = 3.71E-10). In addition, the CBS methylation levels of males and females in the case group were significantly lower than those in the control group (male: 64.33% vs 105%, P = 2.667E-08; female: 78.05% vs 102.8%, P = 0.003). We also found that the CBS levels in the young (23–44), middle-aged (45–59), and older (60–90) groups were significantly lower than those in the control group (young group: 69.97% vs 114.71%; P = 0.015; middle-aged group: 56.04% vs 91.71%; P = 6.744E-06; older group: 81.6% vs 119.35%; P = 2.644E-04). Our ROC curve analysis of CBS hypomethylation showed (Continued on next page) * Correspondence: [email protected]; [email protected]; [email protected] 4Department of Neurology, the Second Affiliated Hospital, Zhengzhou University, Zhengzhou 450014, Henan, China 5Bioinformatics Core, Department of Complementary and Integrative Medicine and John A. Burns School of Medicine, University of Hawai’i, Honolulu, HI 96822, USA 2School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, China Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Li et al. BMC Medical Genomics (2020) 13:163 Page 2 of 10 (Continued from previous page) an area under the curve of 0.713, a sensitivity of 67.4%, and a specificity of 74.0%. Conclusion: Our study suggests that hypomethylation of the CBS promoter may be closely related to the risk of cerebral infarction and may be used as a non-invasive diagnostic biomarker for cerebral infarction. Keywords: Cerebral infarction, DNA methylation, AHCY, CBS, qMSP Background In addition, a meta-analysis of a prospective cohort Stroke is one of the leading causes of death and disabil- study found that after a mean follow-up of 7.3 years, ity [1], with ischemic stroke (cerebral infarction) ac- serum HHcy was reduced by 3 μmol/L, and stroke risk counting for 60 to 80% of all strokes [2]. Cerebral was reduced by 24% [16]. Ashjazadeh et al. found high infarction is a complex disease that is affected by both serum Hcy associated with stroke in the Iranian popula- environmental risk factors and genetic factors [3, 4]. tion [17]. Hankey et al. found elevated serum Hcy in Common risk factors for cerebral infarction include age, stroke patients in Australia as an independent risk factor hypertension, diabetes, hyperlipidemia, smoking, and for ischemic stroke [18]. Hyperhomocysteinemia (HHcy) atrial fibrillation, etc. [5]. DNA methylation is an epigen- is commonly recognized as an independent risk factor etic modification that links the roles between heredity for stroke [19, 20]. and the environment [6]. The mechanism depends on AHCY encodes S-adenosine homocysteine hydrolase the catalysis of DNA methyltransferase (DNMT), which (SAH), whose function is to catalyze the reversible in turn causes the transfer of methyl groups from s- hydrolysis of SAM to produce adenosine (Ado) and adenosylmethionine (SAM) to specific segments in the Hcy (Fig. 1). SAH may control the number of methyl- DNA sequence, thereby changing the function of the ated donors by regulating the level of intracellular genome [7]. Therefore, the level of SAM is closely re- SAM [21]. Plasma Hcy concentrations may increase lated to DNA methylation. Xiao et al. [8] showed that due to genetic defects in related enzymes [22, 23]. PON1 and PON3 methylation were associated with the AHCY methylation may affect the activity of the en- risk of male cerebral infarction. There are several DNA zyme, causing a metabolic disorder of Hcy, leading to methylation studies related to stroke [9]. High homo- HHcy. However, the association between AHCY cysteine (Hcy) leads to hypermethylation of the TM gene methylation and cerebral infarction has not previously and further induction of TM gene silencing, which may been studied. play an important role in the development and progres- In addition, CBS-encoded cystathionine beta-synthase sion of ischemic brain injury [10]. DNA methylation of (CBS) catalyzes the sulfur metabolism of Hcy (Fig. 1). In CDKN2B may play a potential role in arterial calcifica- vivo, Hcy can be irreversibly catalyzed by CBS to pro- tion in patients with cerebral infarction [11]. People with duce cysteine (Fig. 1). At present, many studies have ex- a lower degree of LINE-1 methylation have a higher risk plored the relationship between CBS mutations and of stroke [12]. DNA methylation inhibitor Zebularine stroke, of which T833C point mutation is the most com- confers neuroprotective effects on the ischemic rat brain mon [24–27]. This mutation replaces threonine with iso- and further supports the hypothesis that DNA methyl- leucine, resulting in the inactivation of CBS, blocking transferase promotes delayed ischemic brain injury [13]. Hcy degradation, and ultimately leading to abnormal ac- In the normal fasting state, the human plasma Hcy cumulation of Hcy (HHcy) in the body [28]. concentration is 5–15 μmol. Hcy, as a sulfur-containing The association between AHCY and CBS promoter amino acid in the human body, can be summarized as: methylation and stroke studies has not been reported so (1) further metabolized to cystathionine and cysteine; (2) far. In this study, we examined the levels of AHCY and re-converted to methionine; (3) as a -Based receptors CBS promoter methylation in peripheral blood from pa- participate in choline metabolism; (4) participate in the tients with cerebral infarction and healthy controls. Our folic acid cycle as an essential substrate. Wald et al. have goal is to assess whether promoter methylation of AHCY shown that a 5 μmol/L increase in plasma Hcy leads to a and CBS can be used as a diagnostic biomarker for the 59% increased risk of cerebral infarction; a decrease in risk of cerebral infarction. Hcy of 3 μmol/L reduces the risk of cerebral infarction by 24% [14]. In 2011, the American Heart Association Methods and the Association for Cerebral Infarction jointly issued Sample collection a primary prevention guideline for cerebral infarction, From October 2016 to April 2017, we selected 152 hos- which increases the risk of ischemic cerebral infarction pitalized patients with cerebral infarction from the 960th by two to three times as plasma Hcy levels increase [15]. Hospital of the People’s Liberation Army. Patients were Li et al. BMC Medical Genomics (2020) 13:163 Page 3 of 10 Fig. 1 Hcy metabolic pathway. SAM: S-adenosylmethionine; SAH: S-adenosine homocysteine; Hcy: homocysteine; CBS: cystathionine beta synthase tested by brain CT or MRI, in strict accordance with the ethics committee. In addition, all subjects provided diagnostic criteria of the Fourth National Cerebrovascu- informed consent. lar Disease Conference [29]. The patients included 112 males and 40 females with an age range of 23 to 88 years and an average age of 60.37 ± 12.02 years.
Load more

Recommended publications
  • Senior Women's Performances of Sexuality
    “DUSTY MUFFINS”: SENIOR WOMEN’S PERFORMANCES OF SEXUALITY A Thesis by EVLEEN MICHELLE NASIR Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF ARTS August 2012 Major Subject: Performance Studies “Dusty Muffins”: Senior Women’s Performances of Sexuality Copyright 2012 Evleen Michelle Nasir “DUSTY MUFFINS”: SENIOR WOMEN’S PERFORMANCES OF SEXUALITY A Thesis by EVLEEN MICHELLE NASIR Submitted to the Office of Graduate Studies of Texas A&M University in partial fulfillment of the requirements for the degree of MASTER OF ARTS Approved by: Chair of Committee, Kirsten Pullen Committee Members, Judith Hamera Harry Berger Alfred Bendixen Head of Department, Judith Hamera August 2012 Major Subject: Performance Studies iii ABSTRACT “Dusty Muffins”: Senior Women’s Performance of Sexuality. (August 2012) Evleen Michelle Nasir, B.A., Texas A&M University Chair of Advisory Committee: Dr. Kirsten Pullen There is a discursive formation of incapability that surrounds senior women’s sexuality. Senior women are incapable of reproduction, mastering their bodies, or arousing sexual desire in themselves or others. The senior actresses’ I explore in the case studies below insert their performances of self and their everyday lives into the large and complicated discourse of sex, producing a counter-narrative to sexually inactive senior women. Their performances actively embody their sexuality outside the frame of a character. This thesis examines how senior actresses’ performances of sexuality extend a discourse of sexuality imposed on older woman by mass media. These women are the public face of senior women’s sexual agency.
    [Show full text]
  • The Evolving Perception of Controversial Movies
    ARTICLE Received 6 Jul 2015 | Accepted 2 Nov 2015 | Published 8 Dec 2015 DOI: 10.1057/palcomms.2015.38 OPEN The evolving perception of controversial movies Luca Amendola1, Valerio Marra2 and Miguel Quartin3 ABSTRACT Polarization of opinion is an important feature of public debate on political, social and cultural topics. The availability of large internet databases of users’ ratings has permitted quantitative analysis of polarization trends—for instance, previous studies have included analyses of controversial topics on Wikipedia, as well as the relationship between online reviews and a product’s perceived quality. Here, we study the dynamics of polarization in the movie ratings collected by the Internet Movie database (IMDb) website in relation to films produced over the period 1915–2015. We define two statistical indexes, dubbed hard and soft controversiality, which quantify polarized and uniform rating distributions, respec- tively. We find that controversy decreases with popularity and that hard controversy is relatively rare. Our findings also suggest that more recent movies are more controversial than older ones and we detect a trend of “convergence to the mainstream” with a time scale of roughly 40–50 years. This phenomenon appears qualitatively different from trends observed in both online reviews of commercial products and in political debate, and we speculate that it may be connected with the absence of long-lived “echo chambers” in the cultural domain. This hypothesis can and should be tested by extending our analysis to other forms
    [Show full text]
  • The Musical Number and the Sitcom
    ECHO: a music-centered journal www.echo.ucla.edu Volume 5 Issue 1 (Spring 2003) It May Look Like a Living Room…: The Musical Number and the Sitcom By Robin Stilwell Georgetown University 1. They are images firmly established in the common television consciousness of most Americans: Lucy and Ethel stuffing chocolates in their mouths and clothing as they fall hopelessly behind at a confectionary conveyor belt, a sunburned Lucy trying to model a tweed suit, Lucy getting soused on Vitameatavegemin on live television—classic slapstick moments. But what was I Love Lucy about? It was about Lucy trying to “get in the show,” meaning her husband’s nightclub act in the first instance, and, in a pinch, anything else even remotely resembling show business. In The Dick Van Dyke Show, Rob Petrie is also in show business, and though his wife, Laura, shows no real desire to “get in the show,” Mary Tyler Moore is given ample opportunity to display her not-insignificant talent for singing and dancing—as are the other cast members—usually in the Petries’ living room. The idealized family home is transformed into, or rather revealed to be, a space of display and performance. 2. These shows, two of the most enduring situation comedies (“sitcoms”) in American television history, feature musical numbers in many episodes. The musical number in television situation comedy is a perhaps surprisingly prevalent phenomenon. In her introduction to genre studies, Jane Feuer uses the example of Indians in Westerns as the sort of surface element that might belong to a genre, even though not every example of the genre might exhibit that element: not every Western has Indians, but Indians are still paradigmatic of the genre (Feuer, “Genre Study” 139).
    [Show full text]
  • Everybody Loves Raymond” Let’S Shmues--Chat
    THANKSGIVING WITH THE CAST OF “EVERYBODY LOVES RAYMOND” LET’S SHMUES--CHAT The Yiddish word for “turkey” is “indik.” “Dankbar” is the Yiddish word for “thankful.” By MARJORIE GOTTLIEB WOLFE 46 “milyon” turkeys are eaten each Thanksgiving. Turkey consumption has increased 104% since 1970. And 88% of Americans surveyed by the National Turkey Federation eat turkey on this holiday. Marie Barone (Doris Roberts) is devoted to making sure her boys are well-fed. She gets her adult children back with food. It’s like a culinary hostage trade-off. Marie wants Debra, her daughter-in-law, to be a better cook. She calls her “cooking-challenged” and thinks that she should live directly opposite Zabar’s. Marie want the children to have better food. She says, “A mother’s love for her sons is a lot like a dog’s piercing bark: protective, loyal, and impossible to ignore. Many episodes of “Everybody Loves Raymond” dealt with Thanksgiving. Grab a #2 pencil and see if you can identify which of the following story lines is real or fabricated. Good luck. EPISODE 1. Thanksgiving has arrived. Marie is on a “diete.” She has just returned from a residential treatment facility for weight (“vog”) loss at Duke University in North Carolina. It was an expensive (“tayer”) program. The lockdown began: Low-Fat, No-Sugar (“tsuker”), No Taste foods, and 750 calories a day. (That’s the equivalent of a slice of chocolate cake.) One woman in the program sent herself “Candygrams” each week (“vokh”). Doris eats very little (“a bisl”) at the Thanksgiving table, but as she is preparing to leave Debra’s house, she asks for a little leftover turkey and apple (“epl”) chutney.
    [Show full text]
  • Shuttlegirl Julie Anne Comine Iowa State University
    Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1998 Shuttlegirl Julie Anne Comine Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Creative Writing Commons, and the English Language and Literature Commons Recommended Citation Comine, Julie Anne, "Shuttlegirl" (1998). Retrospective Theses and Dissertations. 14435. https://lib.dr.iastate.edu/rtd/14435 This Thesis is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Shuttlegirl by Julie Anne Comine A thesis submitted to the graduate faculty in partial fulfillment of the requirements for the degree of MASTER OF ARTS Major: English (Creative Writing) Major Professor: Neal Bowers Iowa State University Ames, Iowa 1998 Graduate College Iowa State University This is to certify that the Master's thesis of Julie Anne Comine has met the thesis requirements ofIowa State University Signatures have been redacted for privacy lU for Jeff, with great impatience IV TABLE OF CONTENTS I. The Astronaut's Bride 1 The married planet 2 Reputation 3 hoopskirt 4 The Godmother, part 3 1/2 7 To the memory of Mrs. Seuss 8 Canticle of tentacles 9 The torch singer who came in from the cold 10 Sarcastics anonymous 11 dada 12 Decree 13 Breaking down is hard to do 16 The book of Sarah 17 Hymns & hers 19 Diadem 20 II.
    [Show full text]
  • Mercy Medical Center Community Health Needs Assessment, Two of the Community Statistical Areas Have Shifted
    Mercy Medical Center Community Health June 8 The Sisters of Mercy Needs Assessment & welcome all people of every Implementation 2016 creed, color, economic and Strategy social condition. Community health needs assessments (CHNA) and implementation strategies are required of tax- exempt hospitals as a result of the Patient Protection and Affordable Care Act. The CHNA and implementation strategies create an important opportunity to improve the health of communities by ensuring that hospitals have the information they need to provide community benefits that meet the needs of their communities. They also provide an opportunity to improve coordination of hospital community benefits with other efforts to improve community health. On December 31, 2014, the Internal Revenue Service (IRS) published final rules implementing the “Additional Requirements for Charitable Hospitals” section of the Affordable Care Act (ACA). The hospital facility must "conduct" a community health needs assessment ("CHNA") during the current taxable year or in either of the two taxable years immediately preceding such taxable year and an "authorized body of the hospital facility" must adopt an "implementation strategy" to meet the community health needs identified through the CHNA. Included in this document is Mercy Medical Center’s CHNA and Implementation Strategy as approved by the Mercy Health Services Mission & Corporate Ethics Committee on June 8, 2016. Mercy Medical Center 345 Saint Paul Place Baltimore, Maryland 21201 www.mdmercy.com TABLE OF CONTENTS EXECUTIVE
    [Show full text]
  • Everybody Loves Raymond
    SHOW BIZ Maria Faisal Everybody Loves Raymond Based on the real-life experiences of Ray Romano, Everybody Loves Raymond, the popular sitcom ran from September 1996 to May 2005. Everybody Loves Raymond, a very popular American sitcom broadcast on CBS, ran from 1996 to 2005. The show revolved around the life of Ray Barone, a News Paper sportswriter from Long Island, his wife, Debra, daughter, Ally, and identical twin sons, Geoffrey and Michael. Ray has imposing parents and a jealous, insecure brother, Robert. They are found most of the time in the living room of Ray. Debra, Ray's wife is sick of this routine that had turned her in to a cranky yelling woman, but tolerates for the love of her husband. Ray’s parents and brother never give Ray or his family a moment of peace. Ray often finds himself in the middle of someone else's problems. He is usually the one blamed for everyone else's troubles. Based on the real-life experiences of Ray Romano, Everybody Loves Raymond premiered on September 13, 1996, on CBS. The series finale was broadcast on May 16, 2005, though old episodes are still rerun on cable network TBS and in daily syndication. The show can be watched Monday to Friday on Rogers channel 35, 835, 47 and 847. Paaras 1 Interesting Facts About the Show ·In an unusual turn for such a long-running show, ·Like Robert Barone in Everybody Loves Raymond, every episode featured a single plotline followed Ray Romano has a brother who works for the New throughout both acts.
    [Show full text]
  • Acting Resume
    Chip Chinery COMMERCIALS: AKA TALENT AGENCY: 323-965-5600 THEATRICAL & VOICE OVER: IMPERIUM 7 AGENCY: 323-931-9099 Auburn Hair Brown Eyes SAG-AFTRA www.chipchinery.com Chip’s Cell/Text Machine: 818-793-4329 FILM BATTLE OF THE SEXES Roone Arledge Dir: Jonathan Dayton & Valerie Faris THE OLD MAN & THE STUDIO Co-Star Dir: Eric Champnella PH2: PEARLMAGEDDON Co-Star Dir: Rob Moniot THE COUNTRY BEARS Featured Dir: Peter Hastings COYOTE UGLY Featured Dir: David McNally SPACE COWBOYS Featured Dir: Clint Eastwood ROCKY & BULLWINKLE Featured Dir: Des McAnuff DILL SCALLION Featured Dir: Jordan Brady TELEVISION THE UPSHAWS Malcolm Netflix / Dir. Sheldon Epps THE CONNERS Tony ABC / Dir. Gail Mancuso SHAMELESS Larry Seaver SHOWTIME / Dir. Loren Yaconelli HOMECOMING Sawyer (recurring) AMAZON / Dir. Kyle Patrick Alvarez LIKE MAGIC Club Announcer NBC / Dir. Julie Anne Robinson GOOD GIRLS The Mattress King NBC / Dir. Michael Weaver THE GOLDBERGS Dancing Bruce ABC / Dir. Lew Schneider THE BIG BANG THEORY Bob The Boat Neighbor CBS / Dir. Mark Cendrowski THE KIDS ARE ALRIGHT Bob Parson ABC / Dir. Matt Sohn I FEEL BAD Officer Young NBC / Dir. Tristram Shapeero FAM Race Track Announcer CBS / Dir. Victor Gonzalez NEW GIRL Ben FOX / Dir. Josh Greenbaum LIFE IN PIECES Dustin CBS / Dir. John Riggi BROOKLYN NINE-NINE Don ABC / Dir. Kat Coiro SPEECHLESS Mr. McHugh ABC / Dir. Christine Gernon AMERICAN HORROR STORY Leland Candoli FX / Dir. Brad Buecker NCIS NCIS Agent Rodney Spence CBS / Dir.Tony Wharmby CHELSEA White Collar Comedy Tour Netflix / Dir.Rik Reinholdtsen MOM Dave CBS / Dir. James Widdoes THE MIDDLE Guest Starring ABC / Dir. Blake Evans ANGER MANAGEMENT Guest Starring FX / Dir.
    [Show full text]
  • 'Seven Days in Utopia'
    ‘Seven Days in Utopia’ Life is never the same Once you’ve been to Utopia! Click Here to See SEVEN DAYS IN UTOPIA – Movie Trailer In Theaters August 12, 2011 The Movie ‘Seven Days in Utopia’ was filmed in Utopia Texas, the Utopia Golf Course & the PGA Tour shots were at a Fredericksburg Golf Course. Robert Duvall is Johnny Crawford and Lucas Black is playing Luke Chisolm (the young golfer) Based on the book – ‘Golf's Sacred Journey, 7 Days at the Links of Utopia’ is really a book about life; it just so happens that the main character's life is all about golf. A professional golfer, who is actually never named in the book, has a breakdown on the golf course during a tournament in which he was favored to do well. Despondent and despairing of his future in golf, he wanders into the town of Utopia, where he providentially meets Johnny, a former golf pro who has retired to a quite country life. Johnny, a Yoda-like teacher, who is building a golf course in a field in the middle of nowhere; takes the young golfer under his wing and spends a week completely reshaping the way he thinks about the game of golf and life. The Books web site - http://www.linksofutopia.com/cms In Utopia you can buy the Books at – Hill Country Nature Center & Main Street Utopia Cast: Entire Cast info link - http://www.imdb.com/title/tt1699147 Robert Duvall http://www.imdb.com/name/nm0000380 Lucas Black http://www.imdb.com/name/nm0085407 Kathy Baker http://www.imdb.com/name/nm0000834 Deborah Ann Woll http://www.imdb.com/name/nm2832695 Brian Geraghty http://www.imdb.com/name/nm1310016 Melissa Leo http://www.imdb.com/name/nm0502425 *2011 Best Actress of the Year in ‘The Fighter’ Joseph Lyle Taylor http://www.imdb.com/name/nm0852668 Chris Andersen - Denver Nuggets Basketball Player "The Bird Man".
    [Show full text]
  • DAVID HELFAND, ACE Editor
    DAVID HELFAND, ACE Editor PROJECTS DIRECTORS STUDIOS/PRODUCERS YOUNG SHELDON Various Directors WARNER BROS. / CBS Seasons 1 - 5 Chuck Lorre, Steven Molaro Tim Marx UNITED WE FALL Mark Cendrowski ABC / John Amodeo, Julia Gunn Pilot Julius Sharpe UNTITLED REV RUN Don Scardino AMBLIN PARTNERS / ABC Pilot Presentation Jeremy Bronson, Rev Run Simmons Jhoni Marchinko ATYPICAL Michael Patrick Jann SONY / NETFLIX 1 Episode Robia Rashid, Seth Gordon Mary Rohlich, Joanne Toll GREAT NEWS Various Directors UNIVERSAL / NBC Season 1 Tina Fey, Robert Carlock Tracey Wigfield, David Miner THE BRINK Michael Lehman HBO / Roberto Benabib, Jay Roach Season 1 Tim Robbins Jerry Weintraub UNCLE BUCK Reggie Hudlin ABC / Brian Bradley, Steven Cragg Season 1 Ken Whittingham Korin Huggins, Will Packer Fred Goss Franco Bario BAD JUDGE Andrew Fleming NBC / Adam McKay, Kevin Messick Pilot Will Ferrell THE MINDY PROJECT Various Directors FOX / Mindy Kaling, Howard Klein Seasons 1 - 2 Michael Spiller WEEDS Craig Zisk SHOWTIME Series Paul Feig Jenji Kohan, Roberto Benabib 2x Nominated, Single Camera Comedy Paris Barclay Mark Burley Series – Emmys NEXT CALLER Pilot Marc Buckland NBC / Stephen Falk, Marc Buckland PARTY DOWN Bryan Gordon STARZ Season 2 Fred Savage John Enbom, Rob Thomas David Wain Dan Etheridge, Paul Rudd THE MIDDLE Pilot Julie Anne Robinson ABC / DeeAnn Heline, Eileen Heisler GANGSTER’S PARADISE Ralph Ziman Tendeka Matatu Winner, Best Editing, FESPACO Awards FOSTER HALL Bob Berlinger NBC / Christopher Moynihan Pilot Conan O’Brien, Tom Palmer THAT ‘70S SHOW David Trainer FOX Season 6 Jeff Filgo, Jackie Filgo, Tom Carsey Nominated, Multi-Cam Comedy Series – Mary Werner Emmys CRACKING UP Chris Weitz FOX Pilot Paul Weitz Mike White GROSSE POINTE Peyton Reed WARNER BROS.
    [Show full text]
  • UAB-Psychiatry-Fall-081.Pdf
    Fall 2008 Also Inside: Surviving Suicide Loss The Causes and Prevention of Suicide New Geriatric Psychiatry Fellowship Teaching and Learning Psychotherapy MESSAGE FROM THE CHAIRMAN Message Jamesfrom H .the Meador-Woodruff, Chairman M.D. elcome to the Fall 2008 issue of UAB Psychia- try. In this issue, we showcase some of our many departmental activities focused on patients of Wevery age, and highlight just a few of the people that sup- port them. Child and adolescent psychiatry is one of our departmental jewels, and is undergoing significant expansion. I am par- ticularly delighted to feature Dr. LaTamia White-Green in this issue, both as a mother of a child with an autism- spectrum disorder (and I thank Teddy and his grandmother both for agreeing to pose for our cover!), but also the new leader of the Civitan-Sparks Clinics. These Clinics are one of UAB’s most important venues for the assessment of children with developmental disorders, training caregivers that serve these patients, and pursuing important research outcome of many psychiatric conditions. One of our junior questions. The Sparks Clinics moved into the Department faculty members, Dr. Monsheel Sodhi, has been funded by of Psychiatry over the past few months, and I am delighted this foundation for her groundbreaking work to find ge- that we have Dr. White-Green to lead our efforts to fur- netic predictors of suicide risk. I am particularly happy to ther strengthen this important group of Clinics. As you introduce Karen Saunders, who shares how her own family will read, we are launching a new capital campaign to raise has been touched by suicide.
    [Show full text]
  • 200708 Mu Vb Guide.Pdf
    1 Ashlee 2 Leslie 4 Terri 5 Katie OH Fisher OH Bielski L Angst S Weidner 6 Jenn 7 Tiffany 8 Jessica 9 Kimberley 10 Katie MH Brown MH/OH Helmbrecht DS Kieser MH/OH Todd OH Vancura 11 Rabbecka 12 Julie 14 Hailey 15 Caryn OH Gonyo OH Richards DS Viola S Mastandrea Head Coach Assistant Coach Assistant Coach Pati Rolf Erica Heisser Raftyn Birath 20072007 MARQUETTE MARQUETTE VOLLEYBALL VOLLEYBALL TEAM TEAM Back row (L to R): Graydon Larson-Rolf (Manager), Erica Heisser (Assistant Coach), Kent Larson (Volunteer Assistant), Kimberley Todd. Third row: Raftyn Birath (Assistant Coach), Tiffany Helmbrecht, Rabbecka Gonyo, Katie Vancura, Jenn Brown, Peter Thomas (Manager), David Hartman (Manager). Second row: Pati Rolf (Head Coach), Ashlee Fisher, Julie Richards, Terri Angst, Leslie Bielski. Front row: Ellie Rozumalski (Athletic Trainer), Jessica Kieser, Hailey Viola, Katie Weidner, Caryn Mastandrea. L E Y B V O L A L L Table of Contents Table of Contents Quick Facts 2007 Schedule 2 General Information 2007 Roster 3 School . .Marquette University Season Preview 4 Location . .Milwaukee, Wis. Head Coach Pati Rolf 8 Enrollment . .11,000 Nickname . .Golden Eagles Assistant Coach Erica Heisser 11 Colors ...............Blue (PMS 281) and Gold (PMS 123) Assistant Coach Raftyn Birath 12 Home Arena . .Al McGuire Center (4,000) Meet The Team 13 Conference . .BIG EAST 2006 Review 38 President . .Rev. Robert A. Wild, S.J. 2006 Results and Statistics 41 Interim Athletics Dir. .Steve Cottingham Sr.Woman Admin. .Sarah Bobert 2006 Seniors 44 2006 Match by Match 47 Coaching Staff 2006 BIG EAST Recap 56 Season Preview, page 4 Head Coach .
    [Show full text]