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Supplemental Table 1 Symbol Gene name MIN6.EXO MIN6.M1 MIN6.M2 MIN6.M3 MIN6.M4 A2m alpha-2-macroglobulin A2m Acat1 acetyl-Coenzyme A acetyltransferase 1 Acat1 Acly ATP citrate lyase Acly Acly Acly Act Actin Act Act Act Act Aga aspartylglucosaminidase Aga Ahcy S-adenosylhomocysteine hydrolase Ahcy Alb Albumin Alb Alb Alb Aldoa aldolase A, fructose-bisphosphate Aldoa Anxa5 Annexin A5 Anxa5 AP1 Adaptor-related protein complex AP1 AP2 Adaptor protein complex AP2 Arf1 ADP-ribosylation factor 1 Arf1 Atp1a1 ATPase Na/K transpoting Atp1a1 ATP1b1 Na/K ATPase beta subunit ATP1b1 ATP6V1 ATPase, H+ transporting.. ATP6V1 ATP6v1 ATP6v1 Banf1 Barrier to autointegration factor Banf1 Basp1 brain abundant, memrane signal protein 1 Basp1 C3 complement C3 C3 C3 C3 C4 Complement C4 C4 C4 C4 Calm2 calmodulin 2 (phosphorylase kinase, delta) Calm2 Capn5 Calpain 5 Capn5 Capn5 Cct5 chaperonin subunit 5 Cct5 Cct8 chaperonin subunit 8 Cct8 CD147 basigin CD147 CD63 CD63 CD63 CD81 CD81 CD81 CD81 CD81 CD81 CD81 CD82 CD82 CD82 CD82 CD90.2 thy1.2 CD90.2 CD98 Slc3a2 CD98 CD98 Cdc42 Cell division cycle 42 Cdc42 Cfl1 Cofilin 1 Cfl1 Cfl1 Chmp4b chromatin modifying protein 4B Chmp4b Chmp5 chromatin modifying protein 5 Chmp5 Clta clathrin, light polypeptide A Clta Cltc Clathrin Hc Cltc Cltc Cltc Cltc Clu clusterin Clu Col16a1 collagen 16a1 Col16a1 Col2 Collagen type II Col2a1 Col2 Col6 Collagen type VI alpha 3 Col6a3 Col6 CpE carboxypeptidase E CpE CpE CpE, CpH CpE CpE Cspg4 Chondroitin sulfate proteoglycan 4 Cspg4 CyCAP Cyclophilin C-associated protein CyCAP CyCAP Dnpep aspartyl aminopeptidase Dnpep Dstn destrin Dstn EDIL3 EGF-like repeat discoidin.. EDIL3 EDIL3 Edil3 Eef Eukaryotic elongation factor 2 Eef2 Eef EEF1A1 elongation factor Tu EEF1A1 EEF1A1 EEF1a1 Eif4a1 eukaryotic translation initiation factor 4A1 Eif4a1 Enddo1 Endonuclease domain containing 1 Enddo1 Eno Enolase Eno Eno Enpep glutamyl aminopeptidase Enpep Env Envelope protein Env Env Env F2 Coagulation factor II F2 F2 Fbln1 Fibulin Fbln1 Fbln5 Fignl1 fidgetin-like 1 Fignl1 Flot1 flotillin 1 Flot1 Fn1 Fibronectin Fn1 Fn1 Fn1 Fn1 Fn1 Ftl1 Ferritin light chain Ftl1 Ftl1 Fth1 ferritin heavy chain 1 Fth1 GAPDH glyceraldehyde-3-phosphate dehydrogenase GAPDH GAPDH GAPDH GAPDH GAPDH gag-pol gag-pro-pol polyprotein gag-pol gag gag-pol gag-pol gag-pol Glut1 glucose transporter 1 Glut1 Glut1 Glut1 Gnai2 guanine nucleotide binding protein (G protein) Gnai2 Gnao1 guanine nucleotide binding protein Gnao1 Gnas GTP-binding protein alpha-s Gnas GNPTG N-acetylglucosamine-1-phosphate transferase GNPTG GPI glucose phosphate isomerase GPI Grem1 Gremlin 1 Grem1 Grem1 Gsn Gelsolin, cytosolic Gsn Gstp1 glutathione S-transferase, pi 1 Gstp1 H-2D MHC class I H-2D H-2D H-2K MHC class I H-2K H-2K Hba1 Hemoglobin alpha Hba1 Hba1 Hbb Hemoglobin beta Hbb Hbb Hbb-y Hemoglobin Y Hbb-y Hbb-y Hbb-y Hbb-y Hbb-y Histones Histone clusters Histones Histones Histones Hnrnp heterogeneous nuclear ribonucleoprotein Hnrnp Hras1 Harvey rat sarcoma virus oncogene 1 Hras1 HSP heat shock protein family HSP HSP HSP HSP HSP40 DnaJ HSP40 IGF2 Insulin-like growth factor 2 IGF2 Igsf8 Ig superfamily receptor PGRL Igsf8 Igsf8 Igsf8 Ins2 Insulin II Ins2 ITIH3 inter-alpha trypsin inhibitor ITIH3 ITIH3 Kpnb1 Importin/karyopherin Kpnb1 Krt Keratins Krt Krt Krt Krt Krt Lbp lipopolysaccharide binding protein LDHA Lactate dehydrogenase A LDHA LPL Lipoprotein lipase LPL LPL LPL M6PR Mannose-6-phasphate receptor M6PR M6PR MDH Malate dehydrogenase MDH Mfge8 milk fat globule-EGF factor 8 protein Mfge8 MIF Migration inhibitory factor MIF MVP Major vault protein MVP MVP MYH9 Myosin Heavy chain 9 MYH9 MYH9 MYH9 MYH9 MYH10 Myosin Heavy chain 10 MYH10 MYH10 Myl Myosin light chain Myl Ncl nucleolin Ncl NonO non-POU-domain-containing, octamer binding protein Nsf N-ethylmaleimide sensitive fusion pr Nsf Nsfl1c NSFL1 (p97) cofactor (p47) Nsfl1c Oxtr Oxytocin receptor Oxtr P4hb prolyl 4-hydroxylase, beta polypeptide P4hb Pabp Poly(A) binding protein Pabp Pabp Pabp Pacsin2 protein kinase C and casein kinase substrate Pacsin2 PC1 proprotein convertase Kexin PC1 PC1 PC2 Proprotein convertase Kexin PC2 PC2 PC2 PC2 Pcbp1 poly(rC) binding protein 1 Pcbp1 Pck2 Phosphoenolpyruvate carboxykinase 2 Pck2 Pdcd6ip Alix Pdcd6ip Pdcd6ip Pdcd6ip Pdia6 protein disulfide isomerase associated 6 Pdia6 Pdyn prodynorphin Pdyn PGK Phosphoglycerate kinase PGK PGK Phgdh 3-phosphoglycerate dehydrogenase Phgdh PKM2 pyruvate kinase PKM2 PKM2 PKM2 Plod1 Lysyl hydroxylase 1 Plod1 Plp2 proteolipid protein 2 Plp2 Postn Periostin Postn Postn Postn PPIA peptidylprolyl isomerase A PPIA PPIA Ppp1cc protein phosphatase 1 Ppp1cc Ppp1cc Prdx1 peroxiredoxin 1 Prdx1 Prnp prion protein Prnp Prss1 Protease, Serine 1 Prss1 Prss1 Prss1 Prss1 Psmc2 proteasome (prosome, macropain) 26S Psmc2 Purb purine rich element binding protein B Purb Pzp Pregnancy zone protein Pzp Pzp Rab Rab family Rab Rab Rab7 Rab 7 Rab7 Rabep1 rabaptin, RAB GTPase binding effector protein 1 Rabep1 Rac1 RAS-related C3 botulinum substrate 1 Rac1 Rap1A Rap1A Rap1A Rap1a Rhoc ras homolog gene family, member C Rhoc Rpl11 ribosomal protein L11 Rpl11 Rplp0, l13 ribosomal protein, large, P0 Rplp0, l13 Rps5 5S ribosomal protein Rps5 Rps8, 11, 13Ribosomal protein S Rps8, 11, 13 Rpsa ribosomal protein SA, Laminin Receptor Rpsa Rpsa Scg2 secretogranin II Scg2 Scramp3 Secretory carrier membrane protein 3 Scramp3 Sdcbp Syntenin Sdcbp Sdcbp Sec31a protein transporting Sec31a Sec31a Sec31a Serpinf1 serine peptidase inhibitor, Caspin Serpinf1 Sh3gl1 SH3 domain Grb2-like 1 Sh3gl1 Sh3gl1 Sil1 ER chaperone SIL1 homolog Sil1 Slit1 Slit homology 1 Slit1 Slc16a1 Solute carrier family 16a1 Slc16a1 Slc1a5 Insulin-activated aa transporter Slc1a5 Slc2a3 Solute carrier family 2a3 Slc2a3 Slc38a5 Slc38a5 Slc38a5 Slc7a2 Slc7a2 Slc7a2 Slc7a5 Solute carrier family 7a5 Slc7a5 Slc7a5 Snrpd1 small nuclear ribonucleoprotein D1 polypeptide 16kDa Snx18 sorting nexin 18 Snx18 Spock2 Sparc/osteonectin… Spock2 Spp2 secreted phosphoprotein 2 Spp2 Sub1 RNA polymerase II transcriptional coactivator Sub1 Tagln2 transgelin 2 Tagln2 TFRC Transferrin receptor TFRC Tfrc Tfrc TGFbi TGF beta induced TGFbi Thbs1 Thrombospondin 1 Thbs1 Thbs1 Thbs1 Tln1 Talin 1 Tln1 TPI1 Triosephosphate isomerase TPI1 TPI1 Tpp2 Tripeptidyl peptidase II Tpp2 Tpt1 tumor protein, translationally-controlled 1 Tpt1 Trove2 60KD ribonuceoprotein SSA/Ro Trove2 Trove2 Try4 Trypsin 4 Try4 Try4 Try4 Try10 Trypsin 10 Try10 Try10 trypsinogen trypsinogen7 7 trypsinogen 7 Tsg101 tumor susceptibility gene 101 Tsg101 Tspan6 tetraspanin 6 Tspan6 Tspan7 tetraspanin 7 Tspan7 Tspan7 Tuba alpha-Tubulin Tuba Tuba Tuba Tuba Tuba Tubb beta Tubuln Tubb Tubb Tubb Tub6 Ub Ubiquitin famuily Ub Ub Ub Ub Ub Uba1 ubiquitin-like modifier activating enzyme 1 Uba1 UbE2N ubiquitin-conjugating enzyme E2N UbE2N ValRS Valyl-tRNA synthetase ValRS Vamp2 vesicle-associated membrane protein 2 Vamp2 Vcp Valosin containing protein Vcp Vcp Vcp VcpP97 Chain A VcpP97 VcpP97 VcpP97 Vps25 Vacuolar protein sorting 25 Vps25 Vps28 Vacuolar protein sorting 28 Vps28 Vps37 Vacuolar protein sorting 37B VPS37 Vps37 Vstm2l V-set and transmembrane domain containing 2-like Vstm2l Wars tryptophanyl-tRNA synthetase Wars Ybx1 Y Box binding protein Ybx1 Ybx1 YWHAE YW -monoxygenase act protein YWHAE YWHAZ 14-3-3 YWHAZ SUPPLEMENTAL TABLE. Mass spectrometry analysis of proteins in MIN6.EXO. EXO prepared from MIN6 insulinoma cell culture supernatant (50 µg) was submitted to the mass spectrometry facility in the Scripps Research Institute (San Diego, CA) for protein identification. The result is shown in the third column, MIN.EXO. For column #4-7, total 100 µg of EXO proteins were separated on a 10% Tris-HCl polyacrylamide gel. The gel was divided into 4 slices, M1, M2, M3 and M4, as indicated in Fig. 1B. Each slice was analyzed in mass spectrometry, and protein contents are listed in the rest columns labeled as MIN6.M1, MIN6.M2, MIN6.M3 and MIN6.M4, respectively for each slice. .
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