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Low-Cost Conversion of the Polaroid MD-4 Land Camera to a Digital Gel Documentation System
J. Biochem. Biophys. Methods 67 (2006) 1–5 www.elsevier.com/locate/jbbm Short note Low-cost conversion of the Polaroid MD-4 land camera to a digital gel documentation system Timothy G. Porch *, John E. Erpelding USDA, Agricultural Research Service, Tropical Agriculture Research Station, 2200 Pedro Albizu Campos Ave., Suite 201, Mayaguez, Puerto Rico, 00680-5470 Received 21 April 2005; received in revised form 15 November 2005; accepted 15 December 2005 Abstract A simple, inexpensive design is presented for the rapid conversion of the popular MD-4 Polaroid land camera to a high quality digital gel documentation system. Images of ethidium bromide stained DNA gels captured using the digital system were compared to images captured on Polaroid instant film. Resolution and sensitivity were enhanced using the digital system. In addition to the low cost and superior image quality of the digital system, there is also the added convenience of real-time image viewing through the swivel LCD of the digital camera, wide flexibility of gel sizes, accurate automatic focusing, variable image resolution, and consistent ease of use and quality. Images can be directly imported to a computer by using the USB port on the digital camera, further enhancing the potential of the digital system for documentation, analysis, and archiving. The system is appropriate for use as a start-up gel documentation system and for routine gel analysis. Published by Elsevier B.V. Keywords: Digital camera; Electrophoresis; Gel imaging; Gel documentation 1. Introduction Numerous molecular biology applications rely on the capture of images for the analysis of nucleic acids or proteins. -
Photoflex Catalog.Pdf
Photo by: Ian Spanier PROFESSIONAL LIGHT SHAPING TOOLS 10 Softboxes for Strobe/Cool Lights Softboxes Photofl ex® began producing the LiteDome® softbox in 1985 and remains the world’s most popular softbox for professional photographers. Here are just a few of the features that separate Photofl ex® from the competition: • Our proprietary DuraCloth fabric interior softens light, eliminates leaks, and insures neutral color rendition. • Medium, large and extra large LiteDomes include patented Quick-Release Corners (QRC, see page 6) for easy assembly and break-down, with removable face and interior baffl e. • Optional fabric grid attaches with Velcro® for added control. LiteDome® The LiteDome® is our most popular softbox model due to its simplicity and high performance design. The LiteDome® is the HalfDome® original Photofl ex® white interior softbox model, designed over 30 years ago. Perfect for every SoftBox for strobe only ® photography application, the LiteDome ® consistently delivers even, natural light from The HalfDome features a narrow profi le, your strobe unit. perfect to use as a hair light or rim light for portraits or for getting sleek, elongated Extra Large [XT-4XLLD293] 871150 catchlights in product photography. A Dimensions: 53 x 70 x 35 in. / 134 x 177 x 89cm removable StripMask is included for Large [XT-3LLD293] 871147 creating an even narrower light source, Dimensions: 34 x 45 x 24.5 in. / 86 x 114 x 62cm reducing the dome width by half. Photo by: Scott Stulberg Medium [XT-2MLD293] 871144 Medium [FV-HDMW] 870349 Dimensions: 24.5 x 32 x 17 in. / 62 x 81 x 43cm Dimensions: 15.5 x 55 x 23 in. -
Ten Top Tips for Photography and Videography
PROGRESSIVE APPROACHES TO TI.IE WORK AT I{AND CRU ELTY I NVESTIGATIONS Ten TopTipsfor Good Ph otog ra p hy, Vid eo g ra p hy Document incidents oJ animal cruelty and neglect more eJJectioely by f ollowing tbese belpJul bints. By Ceoffrey L. Handy { ruelty investigators lor the -) Check with local prosecutors Thilor the mix of photographs g - ? SPCA ol Texas in Dallas have Zo and ludges to find out what J o and video to the case at hand. Ltaken to wearing basebali they like and what they dont. Ask Photographs (or "stills") are general- caps on the job. No, they're not be- questions: Do they have any special ly best for stark images, whereas ing unprofessional. Instead, they're requirements or preferences, such as video is best lor capt.uring move- practicing how to use $2,000 worth including dates on photographs, re- ments and sounds. For a ten-month- of undercover surveillance equip- quiring certification by the devel- old German shepherd with a chain ment purchased with a donation oper that the photos were not al- embedded in her neck, for example, from Mary Kay Cosmetics. The tered in any way, or a preference that a few color stills are your best bet. equipment includes a video camera at least one photograph shows the For an animal who vocalizes his dis- small enough to fit inside a baseball investigator on the scene? (To be on tress, supplement the stills with a cap and a f-inch Magnavox televi- the safe side, you should take those minute or two of video. -
The 22” High-Output Beauty Dish Reflector
The 22” High-Output Beauty Dish Reflector What is a High-Output Beauty Dish? Conventional beauty dishes project a very wide angle and low ef- ficiency light output when used with or without a diffusing sock. A direct-light blocking shield is generally necessary because the direct light from the flashtube is very bright in comparison to the light reflected from the white surface of the dish. direct-light blocker assembly The 22” High-Output Beauty Dish is designed to project a narrow 45° beam angle, with about six times the efficiency of a conven- tional dish. This allows for lighting subjects from greater distances with low power flash units, and allows using the reflector with or without the direct-light blocker. It also results in a more selective area of coverage when used closer to the subject. Using the Silver 22HOBD for maximum specularity Beauty Dish with diffusion sock Beauty Dish with direct-light blocker installed Because of its geometry, the Silver Beauty Dish provides a some- Mounting the Beauty Dish what higher degree of shadow softening and the finish allows more specularity than white dishes. Even more specular response can Retract the four retaining fingers by squeezing the levers on top of be achieved by using the Beauty Dish without the included direct- the light (AlienBees) or sliding the lever on the bottom of the light light blocker. Because of its very high efficiency, the ratio of direct over to the release position (White Lightning and Zeus). Remove to reflected light is much lower in comparison to a conventional any mounted reflector, and replace with the Beauty Dish ensuring beauty dish. -
Softbox | Octabox 2 Softbox System
Softbox | Octabox www.broncolor.com 2 Softbox System The broncolor Softbox System Square or octagonal? Let your style decide The new broncolor Softboxes These quality, high-performance light shapers extend your flash system are optimised light shapers for and offer countless additional lighting effects. Three different diffusers precise lighting design in nine enable a soft light with slight central emphasis through to perfectly different shapes and sizes. homogeneous distribution over the entire area. With a light grid, light Square, rectangular, as a strip or control can be even more precise. octagonal. The special internal coating guarantees optimum efficiency. Coloured markings on the tensioning rods and the support ring enable simple, quick erection and dismantling. With appropriate adapters, Softboxes can also be used with flash units from other manufacturers. 3 Softbox System 33.565.00 Softbox 90 x 120 cm (3 x 3.9´) The Big One: Softbox with the classical 3:4 aspect ratio. Perfect for use anywhere where large area and soft lighting is required As with any rec- tangular (but not square) Softbox, you can turn it through 90° to achieve another, slightly different, lighting characteristic. 33.566.00 Softbox 120 x 180 cm (3.9 x 5.9´) The Biggest: Over two square metres of light! Even over the shortest distance this Softbox illuminates a whole body very uniformly and this short distance from the model (or object) guarantees an unbelievably soft and beautiful light. It infuses spaces with a certain magic. 33.564.00 Softbox 30 x 120 cm (1 x 3.9´) The Strip Softbox: The Striplite among the textile Boxes is perfect for illuminating edges in product photography and as a hair-light in the portrait and fashion fields. -
Photo Rentals(Q375 Package)
Photo Rentals (Q375 Package) Power Grip (continued) 1 - 375 amp 3 phase generator 2 - 12 X 12 Solids 2 - 12 X 12 Frame Lighting 1 - 20 X 20 Break Apart Frame 8 - Profoto Pro-7A Pack 1 - 16’ Speedrail 1 - Profoto Pro-7B2 Pack 2 - 10’ Speedrail 8 - Profoto Pro-7 Head 4 - 4’ X 4’ Floppies 1 - Profoto Pro-7 Twin Head 2 - 4’ X 4’ Silks 2 - Profoto Pro-7 Head Extension Cable 1 - 4’ X 4’ Empty Frame 1 - Profoto Ringlight 2 - 24” X 36” Solid 4 - Profoto 41” White Umbrella 2 - 24” X 36” Silk 1 - Profoto 41” Silver Umbrella 2 - 24” X 36” Single 2 - Profoto Magnum Reflectors 2 - 24” X 36” Double 2 - Profoto Magnum Reflector Grids 2 - 12” X 24” Solid 1 - Profoto White Beauty Dish with Diffusion 2 - 12” X 24” Single 1 - Profoto Silver Beauty Dish with Diffusion 2 - 12” X 24” Double 1 - Profoto Beauty Dish Grid 1 - 24” X 60” Cutter 5 - Profoto Grid Sets 10 - 4” Baby Pins 1 - Profoto Pro Globe with Speedring 10 - J Hooks 1 - Westcott 46” Translucent Umbrella 6 - BJ Adaptors 9 - Pocket Wizard Transceivers 2 - Bead Board Holders 2 - Elinchrom 74” Octabank 4 - Bull Pricks 1 - Chimera Super Pro Plus Large White 1 - Sledge Hammer 2 - Chimera Super Pro Plus Medium White 10 - Super Clamps 1 - Chimera Super Pro Plus Small White 4 - Cardellinis End Jaw 1 - Chimera Strip Super Pro Plus Medium White 4 - Cardellinis Center Jaw 1 - Chimera Strip Super Pro Plus Small White 2 - Baby Nail On Plates 6 - Profoto Speedring 6 - Grip Clips #3 2 - ARRI 1K Fresnel 20 - Grip Clips #2 6 - V Flats 10 - Grip Clips #1 4 - 30” Safety Cables 1 - Mega Boom Distro 1 - Mini Boom 1 - -
EZ Lock Beauty Dish Guide Introduction
EZ Lock Beauty Dish Guide Introduction GLOW EZ LOCK BEAUTY DISH is an ingenious portable version of the light modifier famous for striking fashion and portraiture images showcasing the incomparable safe and sure EZ Lock system. No more clumsy construction, fumbling with flex resistant rods or struggling in frustration for careful alignment of speedring holes. The 16-ribbed dish opens up and closes down, ‘umbrella style.’ The quick-to-setup quasi-parabola saucer like assembly has all the advantages of the beauty dish allure without the disadvantages of damage that a metallic dish confronts during location travel and is free of any of the drawbacks of a delicate bendable dish. Intrinsic to the beauty dish design is a small reversible opaque deflector cover that is suspended concave profile out, directly over the front of the light source, preventing direct light from reaching the subject. With the Glow EZ Lock Beauty Dish Eggcrate Grid, you gain more control of a light source’s beam and can feather light for a more attractive shadow, virtually eliminating spill from the rest of the body and the background. Photographers use 25” to 42” beauty dish on monolights and flash heads for flattering people shots, especially for sculpting facial features and creating beautiful shadows and catchlights. When stacked in a vertical tower array of 2 or more, the beauty dish accentuates a model’s full figure with perfect head to toe coverage. 2 GlowLightControl.com Precautions • Please study this guide and store for future reference. • The materials used in this product are not waterproof or flame resistant. -
Fish-Photography-Post-Processing.Pdf
B Spring 2015 American Currents 26 PHOTOGRAPHY AND POST-PROCESSING TECHNIQUES FOR NATIVE FISHES Scott Smith and Jesse Bissette North Carolina Division of Marine Fisheries, Morehead City, NC INTRODUCTION have an older flash knocking about, it will work as well. Just There are many well-documented methods for photograph- know that you will be required to adjust the flash settings ing fishes, using everything from point-and-shoot cameras manually. Price is the second reason for choosing this flash, to professional DSLRs. These methods often produce excel- as a used SB-600 costs about $200. We find this well within lent photographs, albeit with varying background colors our “oops, I dropped it in the water” budget. and different lighting, depending on the weather. In an ef- fort to standardize our photographs, we use an approach Lens that builds off of known methods, consistently provides In order to properly resolve the fins of small fishes, a macro solid black backgrounds, removes any concern over ambient lens is an absolute necessity. We use the Nikon 40mm f/2.8G lighting, and reveals a level of detail not normally seen in AF-S Micro Nikkor on the D3000 and the 60mm f/2.8G photographs of fish. This article aims to outline our process AF-S Micro Nikkor on the D610. Due to the cropped sensor so that anyone, whether equipped with entry-level or profes- of the D3000, both these setups have an equivalent working sional gear, will be able to duplicate it. focal length (i.e., the angle of view is the same). -
Master Professional Portrait Lighting with These 20 Essential Studio Setups
LIGHTING GUIDE Master professional portrait lighting with these 20 essential studio setups REMBRANDT WITH A PORTALITE SOFTBOX REMBRANDT THROUGH AN UMBRELLA REMBRANDT WITH A HONEYCOMB GRID REMBRANDT WITH A SILVER UMBRELLA KIT: One D-lite RX4 head, one Clip-lock KIT: One D-lite RX4 head, KIT: One D-lite RX4 head, KIT: One D-lite RX4 head, Stand, one Portalite Softbox one Clip-lock Stand, one 16cm Reflector, one Clip-lock Stand, one 18cm Reflector one Clip-lock Stand, one 16cm Reflector, Position the light high and to the side to one Shoot-through Umbrella with Honeycomb one Silver Umbrella create a triangle on the model’s cheek. The Position the light high and to the side as with Position the light in the same manner as the Position the light in the same manner as the shadow of the nose should point towards the the ‘Rembrandt with a Portalite Softbox’ previous ‘Rembrandt’ techniques; the light previous ‘Rembrandt’ techniques. The light edge of the lips. The Portalite creates a soft setup. The light is slightly less contrasty, through the honeycomb grid is stronger and bouncing from the silver umbrella is more directional effect. because the light is less directional more dramatic. The grid makes it very easy direct and wraps around the features of the and there is always some reflection to direct the light on to the model and away face yet still creates the shadow from the from the studio surroundings. from the background, which becomes dark. nose towards the mouth. REMBRANDT SHORT REMBRANDT BROAD SPLIT SPLIT WITH FILL KIT: One D-lite RX4 head, one Clip-lock KIT: One D-lite RX4 head, one Clip-lock KIT: One D-lite RX4 head, one Clip-lock KIT: One D-lite RX4 head, one Clip-lock Stand, one Portalite Softbox Stand, one Portalite Softbox Stand, one Portalite Softbox Stand, one Portalite Softbox, one Use the principles of ‘Rembrandt’ lighting Use the principles of ‘Rembrandt’ lighting Position a light to one side of the model in small reflector to create the triangle of light on the face. -
FUSION 2018 the Craft Behind the Image Saturday, May 5Th, 2018 River Rock Casino Resort Richmond, BC
Whatever Your Interests, We Have a Show for You! FUSION 2018 The Craft Behind the Image Saturday, May 5th, 2018 River Rock Casino Resort Richmond, BC Immerse yourself in photography! Fusion 2018 is a full day of speakers, on-stage demos and an Industry Expo. Hear talks on travel photography, portraits, lighting, printing your own images, and more. The Industry Expo will run from 10AM to 5PM with displays and demos of the latest equipment. www.beauphoto.com/fusion2018 An Exhibition of Instant Images April 4th - May 13th, 2018 Opening April 4th, 6 - 8PM Science World - Aurizon Atrium www.beauphoto.com/instant Join the show! Submissions open until March 20th! MAGAZINE Beau Newsletter - March 2018 Beau Photo is On The Move! • The New Fujifilm X-H1 Camera - In Stock and in Rentals! • Fujifilm XF 80mm f/2.8R LM OIS WR Macro lens • Canon Speedlite 470EX-AI • Contax T Cameras • All About Fujifilm Instax Cameras • New Lastolite Skylite Rapid System • Renaissance Stock Albums Sale • more... BEAU NEWS MARCH 2018 Beau Photo Is On The Move... City of Vancouver Archives - CVA 99-3766 - Arrow Transfer fleet of trucks We are excited to announce that the rumours are true: Beau Photo will be on the move in late 2018! This is something that’s been in the works for well over a year, and the plan is now coming together. We are in the process of securing a new location and once everything is finalized, we’ll let you know. With even more developments scheduled in the area of our current location, the time is ripe for us to head elsewhere and we’re sure you won’t miss the parking struggle! Once we have finalized the where and when, you’ll want to “Save the Date” for the big Welcome to our New Store Party we have planned! We are all excited about designing a new space in a new location and please note, the plans include not having the shipping/receiving area and staff kitchen in the middle of the shop this time around! But don’t worry, our unique, laid-back shopping experience will still be there. -
Summer Is Here!
Summer is Here! We’ve been talking about it for three seasons, but summer has Valid from June 21 finally arrived! Get the inspiration and gear you need inside. to June 27, 2019 PHOTO BY TOM-GRIMBERT ON UNSPLASH TOM-GRIMBERT BY PHOTO 15,000 PRODUCTS IN-STORE OR ONLINE AT HENRYS.COM +BONUS! VANGUARD BAG $40 VALUE CANON EOS M6 NIKON D5600 SONY ALPHA A6000 Includes 15-45mm IS lens Includes AF-P 18-55mm VR lens Includes 16-50mm lens • 24.2 MP CMOS (APS-C) sensor • Easy to use with full creative control • Compact body • Fast, accurate dual pixel CMOS AF • Nikon Snapbridge wireless • Built-in electronic viewfinder • 5-axis image stabilization smartphone connected • 11 fps continuous shooting $ 99 SAVE $550 $ 99 SAVE $350 $ 99 SAVE $180 599 567CAN159 699 NIKKIT605 599 567SON066 HENRY’S EXCLUSIVE SONY CYBERSHOT HX80 PANASONIC LUMIX FZ300 Compact Camera Compact Camera CAMERON OBSIDIAN SERIES SLING BACKPACK • 30x Zeiss zoom • 24x optical zoom • Electronic viewfinder • 4K video/4K photo • DSLR or mirrorless cameras, • Built-in Wi-Fi • Splash/dustproof 2-3 lenses, and accessories $399.99 SAVE $50 566SON432 $499.99 SAVE $150 566PAV269 $49.99 SAVE $30 147CAM013 - CANADA'S GREATEST CAMERA STORE PAGE 1 Stay gold Golden hour, an hour after dawn and before sunset, SHOP IN-STORE OR ONLINE AT provides a soft, magical light for your subjects. HENRYS.COM PHOTO BY MASON-WILKES ON UNSPLASH MASON-WILKES BY PHOTO +BONUS! SONY 64GB SD CARD $100 VALUE SONY DSC-RX100 SONY FDR-AX53 SONY DSC-RX100 VA Premium Compact Camcorder Premium Compact • 1-inch Exmor CMOS sensor -
2.2 Producción Audiovisual
FACULTAD DE DISEÑO ARQUITECTURA Y ARTE ESCUELA DE DISEÑO DE OBJETOS SISTEMA DE HERRAMIENTAS PARA REGISTROS AUDIOVISUALES Y MULTIMEDIA TRABAJO DE GRADUACIÓN PREVIO A LA OBTENCIÓN DE TITULO DE DISEÑADOR DE OBJETOS AUTOR: ANTONIO AGUIRRE CORONEL TUTOR: ING. JOSÉ LUIS FAJARDO SEMINARIO, MGST CUENCA - ECUADOR 2020 Í AUTOR: Antonio Aguirre Coronel TUTOR: Ing. José Luis Fajardo Seminario, Mgst Cuenca - Ecuador 2020 7 DEDI Quiero dedicar este proyecto a mi familia, amigos, profesores, profesio- nales y aficionados en el área de la fotografía y vídeo, quienes se han “ visto en la necesidad de optimizar recursos para facilitar la realización de producción audiovisual en diferentes ámbitos, planteando una nueva alter- CATO nativa multifuncional que responde a una serie de requerimientos dados un espacio determinado. ” RIA 8 9 Agradezco de manera especial a mi querida madre quien ha sido incondi- cional en mi vida, y pilar fundamental de la misma, a mi padre que en paz “ descanse, y aunque hoy ya no este presente fue quien me enseñó a saber tomar decisiones en la vida y quien me motivó a cumplir cada uno de mis metas, también agradezco a mi familia, quienes me han apoyado a AGRA lo largo de este proceso de formación a pesar de las dificultades y se han convertido en mi fuente de inspiración, les doy las gracias por no haberme dejado decaer, sino al contrario siempre me motivaron a ser responsable y lograr uno a uno mis propósitos. DECIMI Finalmente les doy las gracias a mis profesores que me han compartido sus conocimientos y han sido aporte fundamental para mi formación como profesional incluyendo a todas las personas que han creído en mi y mis capacidades, las mismas que han servido como un granito de arena para ENTO lograr concluir este proyecto.