F - 1 CROP PROTECTION PLANT PATHOLOGY

Research Findings:

Path.1 Epidemiological studies on cotton diseases

Path.1 (a): Observations on the occurrence of various diseases (all centers except Pune and CICR, Sirsa)

North Zone

Cotton leaf curl disease (CLCuD), Bacterial Blight (BB), Fungal Foliar leaf spots (FFLS) were the diseases observed in different districts of Punjab by Faridkot center. The PDI of CLCuD varied from 0.1 to 89.2%. 40 to 77% ; 37 % to 65 % on varieties/hybrids like RST-9, RS 921, F 846, F 505, F 1378, F 1861 , F 2383, F 2228, RCH 650 Bt, Bioseed 6488 BGII , Bioseed 6588 BGII and RCH 773 BGII in different villages & research farm of Faridkot, Fazilka and Muktsar districts/ area (Table-1). Bacterial blight was found in 0.1 to 2.9% in Faridkot and traces to 2.1% in Fazilka and traces to 2% in Muktsar on hybrids like Bioseed 6488 BGII, Bioseed 6588 BGII and various entries of coordinated trials in different cotton growing areas. The fungal foliar leaf spots caused by Alternaria, Myrothecium and Cercospora, ranged from 0.3 to 2.6%, traces to 2.4% and traces to 2.1% disease intensity on RST-9, RS 921, F 846, RCH 650 BG II and RCH 773 BG II and different entries of coordinated trials at research farm. The incidence was also observed in the different districts of Punjab i.e. Bathinda, Mukstar and Mansa by Bhatinda center. Incidence of bacterial blight, fungal foliar leaf spot and root rot was noticed in traces and reported in Bathinda district only. The data (Table-2) revealed that the PDI of CLCuD varied from 25.33-72.67, 16.67-51.33 and 31.33-56.67 respectively in different villages of district Bathinda, Mansa and Muktsar. Highest PDI was recorded in US Agriseeds US 21BG II as 72.67 in Village Jodhpur of district Bathinda.

Out of four tehsils surveyed in Sriganganagar district of Rajasthan, Sriganganagar tehsil, was found to be severely invaded by leaf curl virus showing average disease intensity of 31.1 per cent. In Srikaranpur and Padampur tehsils the average disease intensity was 5.2 and 2.7 per cent, respectively whereas incidence was not observed in Suratgarh tehsil. In adjoining cotton belt of Hanumangarh district, the average disease intensity was 6.1 per cent (Table-3).

The survey in Haryana was undertaken to record the occurrence of different diseases in major cotton growing districts (Sirsa, Hisar, Fatehabad, Jind and Bhiwani) as well as in the non- traditional areas of Haryana (Kaithal, Rohtak, Mahendergarh and Rewari) from July to September, 2015. At farmer’s field highest per cent disease index (PDI) of 58.3 (Ankur 3028 BG II), 50.0 (Bio 6588BGII), 46.6 (RCH 602 BG II) and 43.3 (RCH 650 BG II) were observed in villages Siswal, Danoda, Dhingsara and Darba Kalan of districts Hisar, Jind, Fatehabad and Sirsa respectively. In Bhiwani district disease appeared in the month of September up to 21.7 PDI. Whereas, Mahendergarh, Rewari and Palwal districts were free from CLCuD. At CCS HAU research farm PDI of CLCuD was observed from 4.8 to 66.6 in different cultivars. Bio 100 showed resistant reaction against CLCuD with the lowest PDI of 4.8. Among the fungal foliar diseases, Myrothecium leaf spot (MLS) PDI

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 2 varied from 0.0 to 25.0%. Other diseases viz. Alternaria leaf spot (ALS), root rot (RR) and Bacterial leaf blight (BLB) did not appear at farmer’s field on all locations surveyed in Haryana (Table-4).

Central Zone

Alternaria leaf blight was the major disease observed during the year on research farm at Rahuri in Maharashtra. The disease intensity irrespective of variety/hybrids ranged from 4.25 to 20.70 per cent (Table-5) and it reduced from October onwards. The other foliar diseases viz., Myrothecium, Cercospora, grey mildew and bacterial blight were observed in traces during crop growth. The incidence of Tobacco streak virus was very low i.e. only upto 3% which was mainly observed as localized infection on upper leaves of the plants. The sowing of Bt cotton under irrigated areas was done from 15th May onwards, but the water stress conditions resulted in high incidence of the physiological disorder - para wilt. Due to low rainfall, the sowing of rainfed cotton (Bt/non-Bt) was also delayed up to first fortnight of July (28th MW) in most part of Maharashtra. Alternaria blight was the major disease observed and the disease intensity ranged between 2.15 to 23.04 per cent irrespective of variety/hybrids and locations. The other foliar diseases were observed in traces. In the current year very low rainfall than the average was received in most of the areas of Maharashtra during Kharif season, which resulted in the drought conditions and low productivity of seed cotton. The normal sowing of cotton was also delayed up to 20th July on farmer’s field.

The Alternaria was found wide spread disease followed by bacterial blight and grey mildew on G. hirsutum and G. arboreum cotton in the areas surveyed around Nanded, Maharashtra. The intensity of Alternaria varied from 6.50 to 21.50 PDI, while the intensity of bacterial blight ranged from 2.50 to 28.50 PDI; grey mildew intensity varied from 0.00 to 3.00 PDI. The incidence of para wilt varied from 0.00 to 3.00 PDI, during this year (Table-6).

During seedling stage crop was free from diseases at farmers and research field at Akola, Maharashtra. The bacterial blight was observed in flowering stage varied from 1.01 to 4.08 and 0.33 to 2.56 per cent disease intensity on farmers and research field. Maximum disease intensity was observed at boll development stage 5.41 and 5.54 per cent in varieties/Bt hybrids at farmers and research field respectively. The grey mildew intensity was not observed on farmer field and research farm. The Myrothecium leaf spot was observed at flowering stage within range of 0.81 to 2.02 and 0.72 to 1.72 per cent on farmers and research field respectively. Alternaria leaf spot was not observed during crop season. (Table-7)

Myrothecium leaf blight of cotton was first observed in II week of August in Khandwa. In the month of September the disease incidence increased up to 18.8%. Bacterial blight was observed in 2nd week of July. The incidence increased up to 15.2 %. Grey mildew was first observed in second last week of September. The disease incidence increased up to 12.7 %.On the farmer’s fields where cotton sown in early summer Bacterial Leaf Blight and Myrothecium leaf blight was observed in 3rd week of July. The incidence of New Wilt in Bt hybrids has not been observed in Research Farm and farmer field in vicinity of Khandwa.

On Research farm at Surat, Gujarat, periodic observations recorded on G. Cot. Hy. 12 revealed that bacterial blight appeared during the 2nd week of July (2.0 %) and then gradually developed and reached at its peak (23.5 % PDI) during the 3rd week of September and then

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 3 declined, but prevailed up to 1st week of December (Table 8). Observations were also recorded on other cultivars. The susceptible cultivars viz., LRA 5166 showed bacterial blight intensity to the tune of 2.5 to 23.0 per cent PDI moreover, non Bt cotton was more susceptible to the bacterial blight disease. During 30 - 45 DAS (vegetative stage of the crop), the BLB was more pronounced on lower leaves. Roving survey was conducted in Surat, Bharuch and Narmada districts on farmers’ field. The BLB incidence was found in the range of 0.0 to 5.5 %, 0.0 to 5.5 % and 0.0 to 24.0 % PDI in Surat, Bharuch and Narmada districts, respectively on cultivars fields in majority growing approved BG II hybrids. Moreover, Alternaria leaf spot was observed in the range of 0.0 to 13.5 % in Nandod taluka of Narmada district (Kuta Amba village). Whereas, in other two districts viz., Surat and Bharuch, it was in the tune of 0.0 to 4.5 % and 0.0 to 2.5 % PDI. The disease incidence was also recorded in the HDPS experiment under TMC MM 1 project at Surat which revealed that all the four genotypes were found Moderately resistant to BLB disease with the Grade 2 and Alternaria leaf spot diseases was not observed. (Table-8a)

Survey on the occurrence of various cotton diseases was carried out on farmers field and cotton research station JAU Junagadh, Gujarat. Total of 29 locations covering 7 districts namely, Junagadh, Amreli, Surendranagar, Jamnagar, Rajkot, Gir Somnath and Porbander were intensively surveyed. Bacterial blight was recorded during flowering stage while Alternaria leaf spot recorded in almost all locations during boll formation (Table-9). The alternaria leaf spot (ALS) recorded in Junagadh district in different varieties ranging from 8.0 to 17.0, and bacterial leaf blight (BLB) 1.0 to 3.0 per cent disease intensity (PDI). In Amreli district, ALS disease was found in the ranging from 11.0 to 19.0 and BLB 1.0 to 2.0 per cent. In Surendranagar district ALS disease noted to the ranging of 14.0 to 15.0 and BLB 0.0 to 1.0. In Jamnagar district ALS disease noted in range of 0.0 to 17.0 and BLB 0.0 to2.0 per cent. Disease condition in Rajkot district, ALS was noted in range of 13.0 to 19.0 and BLB 0.0 to 1.0 PDI. In Gir Somnath, ALS and BLB disease recorded in the ranging of 7.0 to 11.0 and 0.0 per cent, respectively. In Porbander district ALS and BLB disease incidence recorded in the range of 9.0 to 18.0 and 0.0 to 1.5 PDI, receptively. At cotton research station, Junagadh varieties G. Cot.hy-6 and G. Cot hy-18 recorded ALS 16.0 and 17.0; BLB 4.0 and 3.0 percent respectively. During the season Grey Mildew was not observed in all locations, while root rot and wilt were observed negligible in some locations.

South Zone

Survey results from Dharwad, Karnataka indicated the predominance of Alternaria blight (05-30%) in early stages of the crop growth followed by Grey mildew (05-30%) and Bacterial blight (10-25%). Rust appeared after 90 DAS (20-40%). (Table-10). The results of incidence of Cotton foliar diseases as influenced by technology for Organic cotton production (G. arboreum) indicates that, the treatment no.8 (RDF through inorganic fertilizers i.e. 60:30:30NPKkg/ha)has least incidence of Alternaria blight and rust with higher yield (Table 10 a).

Alternaria leaf spot, bacterial blight, grey mildew and rust were recorded during the surveys conducted from September to December 2015, in cotton growing districts of Andhra Pradesh viz., Guntur, Praksam, Anathapur, Kurnool, Kadapa and Krishna (Table11 ). Intensity of bacterial blight, Alternaria leaf spot, grey mildew and rust diseases in various hybrids and varieties of cotton trials conducted at RARS, Lam was also recorded. During August and September traces of Bacterial blight was recorded in Jadoo BG II in Brahmanapalle village, Guntur district during November 2015 (8.5 PDI) and Virupapuram village, Kurnool district during December 2015 (18.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 4 PDI). Traces of root rot were observed in Jadoo BG II during September 2015 at Ravela village and in RCH 659 BG II at Kesanupalli village, Guntur district during October 2015. Traces of Boll rot was recorded during September 2015 in Bhakthi BG II at Krishnapuram village, Krishna district. Cercospora leaf spot was (11.2 PDI) observed in Jadoo BG II during October 2015 at Tallacheruvu village, Krishna district. Alternaria leaf spot, grey mildew and rust diseases were recorded to the tune of 0 to 21.3%, 0 to 18% and 0 to 16.3% in farmers’ fields surveyed during September to December. Low to moderate intensity of Alternaria leaf spot and rust were observed at Regional Agricultural Research Station, Lam, and Guntur while grey mildew recorded moderate to severe intensity.

Survey was conducted to assess the severity of cotton diseases from regions viz., Dharapuram, Tirupur, Erode, Anthiyur, Thondamuthur, Ariyalur and Coimbatore of Tamil Nadu. The incidence of diseases viz., root rot, wilt, Alternaria leaf blight, Rust, grey mildew, bacterial blight and TSV were assessed in the above mentioned cotton growing regions. Survey results indicated that the foliar diseases viz., rust, BLB and grey mildew was not found to occur till harvest in surveyed areas except Coimbatore where the incidence of the diseases was observed. The incidence of Alternaria leaf blight ranged from 2.0 to 8.0 PDI. The maximum incidence was observed in Coimbatore district. Survey for the occurrence of root rot indicated that the incidence varied from 5.0 to 18.0%. The maximum incidence of root rot was observed to an extent of 18.0% in Coimbatore district. The incidence of both wilt and root rot was found to be higher in diploid cotton. The severity was more when the disease was found to occur along with the stem weevil incidence. Survey for the occurrence of viral diseases revealed that Tobacco Streak Virus was found to be observed in all cotton growing tracts surveyed in Tamil Nadu. Incidence of TSV varied from 2.0 to 11.0%. The maximum TSV infection of 11.0% was recorded in Coimbatore district (Table-12).

1(b): Disease progress in relation to weather factors (all centers)

NORTH ZONE:

Cotton leaf curl viral disease (CLCuD) was observed on susceptible variety RS 921 in 26th meteorological week (25-01 July) at Faridkot. Observations on the appearance and progress of CLCuD were taken at weekly interval starting from 25th upto 44th meteorological week. It was found that CLCuD % increased upto 42nd meteorological week (87.2). The bacterial blight started late in the season in 30th meteorological week with 0.1 % DI. The fungal foliar leaf spots (caused by Alternaria, Myrothecium, and Cercospora) ranged from 0.1to 2.8; 0.1 to 2.6 and 0.3 to 2.6 disease index from 30th to 42nd meteorological week (Table-13). Co-efficient of correlation [r] for CLCuD %, Percent CLCuD increase was positively significant with white fly population, relative humidity and min temperature and rainfall. Co-efficient of correlation [r] of per cent disease index (PDI) of bacterial blight (BB) was positively correlated with wind direction, bss and negatively co related with rainfall, maximum and minimum temperature. Similar type of Correlation of per cent disease index (PDI) of Myrothecium leaf spot (MLS), Alternaria leaf spot (ALS) and Cercospora leaf spot (CLS) was observed in all the three fungal disease.

Leaf curl first appeared on 26th June after 43rd day of sowing during 26th St. Week and the incidence at the end of this week was only 0.47 per cent at Sriganganagar. However in subsequent standard week’s leaf curl incidence in crop showed fast progressive increase reaching maximum level of 100.0 per cent on 29th Aug. during 35th Standard Week. Data presented in (Table-14)

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 5 indicate that disease remained slow up to 27th Std. Week (2 July – 8 July) with disease severity of 9.80 per cent. In subsequent 28th to 30th std. week disease increased about three fold reaching to the level of 66.17 per cent. Fast progress of disease was observed up to 31th Std. Week (30 July – 05th Aug) and the leaf curl incidence reached to 95.26 per cent. Thereafter progress of disease was slow between 32rd (06th Aug – 12th Aug.) to 35th (27th Aug – 02th Sept) standard week. During this period progress of disease was between 96.73 to 100.0 per cent only. The overall precipitation from June to August (vegetative phase) during this year was 294.8 mm. The maximum average monthly temperature during this period of June to August remained between 37.80 and 26.02 oC and Relative humidity between 55.3 and 76.2 per cent (Table14a).

CLCuD appeared on 9th June, 2015 in the 23rd standard meteorological week after 17 days of sowing and disease incidence increased up to 100 % in 27th week (2.7.15 to 8.7.15) at Hisar. During this period the average maximum and minimum temperature were 38.1 and 25.1oC, whereas, morning and evening relative humidity ranged from 73.5 to 48.2 per cent with 7.5 bright sun hours having whitefly population 47.2 per three leaves. However, 1.0 PDI was observed in 23rd standard meteorological week, which increased up to 55.9 in 31st standard meteorological week (Table-15). The per cent leaf curl disease data of cotton (2005 to 2015) were correlated with the meteorological parameters. The maximum temperature (-0.58), min. temperature (-0.48), wind speed (-0.66), evaporation (-0.65), actual vapour pressure at morning (-0.29) & evening (-0.25), evening relative humidity (-0.01), and rainfall (-0.06) showed negative correlation with disease development, whereas, morning relative humidity (0.43), sunshine hours (0.19) and cumulative rainfall (0.68) showed positive correlation. The optimum temperature for the disease development was noted to be between 33 to 37°C, RH m 77 to 90 percent, evaporation 2.9 to 7.1 mm, wind speed 1.9 to 5.3 km per hours, bright sunshine hours 4.3 to 8.2 hrs. The wind speed showed a highly significant negative correlation with disease development which may be favorable for white fly migration. There was an exponential relationship between disease development and cumulative rainfall (sigmoid growth curve) i.e. the rate of disease development was initially slow till the accumulation of 100 mm rainfall and thereafter, disease development increased sharply. The sunshine hours showed a linear relationship with disease indicating that the clear days favored its development. Stepwise multiple linear regression process was carried out to identify best suited model using above weather variables.

The leaf curl virus disease initiated in 24th met week (11-17 June) and the incidence reached 100% by 34th week (20-26 August) at Sirsa. An increasing trend of whitefly incidence from 1.75 per 3 leaves upto 70 whiteflies per 3 leaves was observed during the season. The maximum temperature ranged from 31.8 to 39.30C whereas minimum temperature was between25.5 to 27.80C. Relative humidity morning and evening ranged between 66.9-92 and 39.1-76 % respectively. The rainfall received during this period was 157.8 mm. (Table-16).

CENTRAL ZONE:

The first incidence of Alternaria blight was noticed on 20th July (29 MW) on research farm at Rahuri. The average maximum and minimum temperature during 28th MW was 34.60C and 23.60C, respectively and average morning and evening humidity was 67.9 and 41.3 per cent, respectively with 0.0 and 17.4 mm rainfall in 28th and 29th MW, respectively. The intensity of Alternaria blight increased at slower rate from 2.9 per cent in 29th MW to 20.7 per cent in 38th MW.

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 6 The average maximum and minimum temperature during 37th MW was 31.30C and 22.00C, and average morning and evening humidity was 78.9 and 58.1 per cent, respectively. As per the correlation there was significant positive correlation of rainfall (at 1 %) with Alternaria blight development. Also, the minimum temperature had significant negative correlation ( 1 & 5 %) with the Alternaria blight development. The other weather parameters i.e. morning and evening humidity, sunshine hours and rainy days had positive and maximum temperature negative correlation with PDI of Alternaria blight in cotton (Table17 and 17a).

Bacterial blight was initiated during first week of September 2015 (36 MW), when the maximum and minimum temperature was 32.9 0C and 22.5 0C along with relative humidity between the range of 87 and 57 percent at Akola. Later gradually increased and maximum per cent disease intensity i.e. 8.6% was observed during (41 MW). Maximum and minimum temperature was 37.0 0C and 19.3 0C with relative humidity range of 71 - 24 percent. Later decreasing trend was observed (Table-18). In LRA-5166 as well as Bunny Bt, similar trend was observed. Myrothecium leaf spot diseases was initiated during first week of September 2015 (36 MW), during this year and maximum disease intensity i.e. 2.08% was observed during (37 MW). Maximum and minimum temperature was 34.3 – 27.20C along with relative humidity between the range of 88 and 54 per cent in LAR 5166 and Bunny Bt respectively. Grew mildew was not observed on LRA 5166, Bunny Bt and AKA-8 throughout the season. The data presented in Table-18a indicated that the correlation between rainfall, minimum temperature and relative humidity morning and evening showed negative and non-significant at both the level of significance with disease whereas maximum temperature and sunshine hours has showed positive and significant correlation with bacterial blight disease at both levels of significance.

On susceptible cultivar JK-4 bacterial blight was first observed in traces on 25th Standard Meteorological Weeks (SMW) at Khandwa. The disease intensity gradually increases upto35th SMW and then decreased up to 43th SMW. From the last week of August disease increases and in 35th SMW incidence was maximum, the grade was 3.0 in 0-4 scale. Myrothecium leaf disease was also first noted in 25th SMW and increased up to 35th SMW. The incidence of Grey Mildew was first noted in 39th SMW and increased up to 43th SMW, the grade was 2.0. (Table-19).

Bacterial blight disease (BLB) progress was recorded with its first appearance and subsequently at weekly interval till it prevailed on G. Cot. Hy.12 on Research farm at Surat. The result presented in Table, indicated that the disease first appeared in 2nd week of July (2.0 %) i.e. in 28th Met. week. The incidence of bacterial blight disease (BLB) was noticed from 28 to 49th standard week with the maximum disease intensity in third week of September (23.5 %) i.e. in 39th Met. week. Non significant correlation with all the parameters (Morning & Evening humidity, Max temperature, Rainy days and Rainfall) was found for the disease development (Table-20).

The first occurrence of Alternaria Leaf Spot was observed during third week of September (39thSMW) at Junagarh. Highest percent disease incidence of 17.20 was recorded during second week of November (46th SMW). The first occurrence of BLB was recorded during second week of August i.e. 32nd meteorological standard week. Maximum (9.40 %) BLB incidence was recorded during first week of December (49th SMW) and there after it declined. The grey mildew disease remain absent during the season (Table-21). The data presented in Table reveled that Alternaria leaf spot disease found non significant with maximum temperature, rainfall and rainy days. The minimum temp and relative humidity (%) at morning and evening were recorded significant and

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 7 negative correlation at 1 per cent level. The maximum temperature, rainfall and rainy days found negative correlation at 5 per cent level. Bacterial leaf blight disease correlation coefficient studies recorded non significant with maximum temperature, rainfall and rainy days. The minimum temperature and relative humidity morning and evening found negative correlation at 1 per cent level. While maximum temperature, rainfall and rainy days found negative correlation at 5% level. The Grey mildew disease was not observed during the season (Table21a).

SOUTH ZONE:

Maximum Alternaria blight incidence(PDI) of 32.2,28.7,32.9 & 34.4 was noted in 46th met week at Dharwad farm in Abadhita, Jayadhar, Bunny Bt and RCH 2 Bt. Bacterial blight was noted upto 11.9, 7.2 and 8.0 in Abadhita, Bunny Bt and RCH 2 Bt respectively. In case of Grey mildew a maximum of 12.7, 26.8, 4.4 & 7.3 PDI was noted. Whereas rust incidence was 23.0, 13.9, 30.5 & 20.8 respectively in above referred varieties/hybrids at 46th met week. (Table-22 and 22a).With respect to Alternaria blight, bacterial blight, grey mildew and rust diseases significant positive correlation was observed between the maximum temperature and the genotypes. Significant negative correlation or no correlation was observed between minimum temperature and genotypes in general. No correlation was observed between RH Morning and RH Evening in four genotypes with respect to four diseases. Significant positive correlation was noticed between total rainfall and rust in all the genotypes and no correlation was noticed between rainy days and the genotypes with respect to all the four diseases (Table-22b).

The data recorded at Guntur showed that Alternaria leaf spot appeared in 36th standard week during September 2015 when the mean maximum temperature was 33.30C; mean minimum temperature was 24.90C; mean relative humidity of 88%; mean rainfall was 93.8mm; rainy days were 4.86; sun shine hours were 5.83; wind speed 4.86kmph and evaporation was 7.43. Alternaria leaf spot attained a maximum PDI of 30.0 on Jadoo BG II during 48th standard week i.e., last week of November, during which the mean maximum temperature was 30.930C; mean minimum temperature was 18.60C; mean relative humidity of 98.3%; shine hours were 4.7; wind speed 4kmph and evaporation was 6.7 RCH 2 BG II and Bunny BG II recorded 21 and 20 PDI, respectively, during the same period (Table-23).Grey mildew disease appeared in 44th standard week during November 2015 on Jadoo BG II when mean maximum temperature was 35.50C; mean minimum temperature was 23.60C; mean relative humidity of 92%; sunshine hours were 6.3, wind speed 4.7kmph and evaporation was 9.7. The disease progressed and reached PDI of 52.5 on Jadoo BG II during 51st week of 2015 during which the mean maximum temperature was 32.00C; mean minimum temperature was 16.90C; mean relative humidity was 97.5%; sunshine hours were 3.38, wind speed 3.88kmph and evaporation was 3.0. Disease intensity was negatively correlated with maximum, minimum temperatures, RH II, Rainfall, rainy days, wind speed, evaporation; positively correlated with RH I and sun shine hours Significant correlation was recorded with temperature, RH I, number of rainy days, wind speed and evaporation. Multiple linear regression analysis revealed that minimum temperature and rainfall significantly influenced the disease intensity. In Bunny BG II grey mildew appeared later in 48th SMW during which the mean maximum temperature was 30.930C; mean minimum temperature was 18.60C; mean RH of 98.3%; shine hours were 4.7; wind speed 4kmph and evaporation was 6.7. The disease progressed and reached PDI of 34.0 during 51st week of 2015 during which the mean maximum temperature was 32.00C; mean minimum temperature was 16.90C; mean RH was 97.5%; sunshine hours were 3.38, wind speed 3.88 kmph and evaporation was 3.0 (Table 1b.1). Positive correlation was recorded with maximum

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 8 temperature, SSH and wind speed, while it was significant for max temperature. Minimum temperature, RH and evaporation showed negative and non significant correlation. Multiple linear regression analysis revealed that temperature and RH II significantly influenced the disease intensity. Rust disease appeared in 49th standard meteorological week during December 2015 when mean maximum temperature was 31.90C and mean minimum temperature was 18.30C, and mean RH of 99.4%. The disease progressed and reached PDI of 45 on RCH 2 BG II during 4th week of 2015 during which the mean maximum temperature was 30.70C; mean minimum temperature was 18.210C and mean RH was 96%. Jadoo BG II and Bunny BG II recorded 41% and 35% during the same week. Disease intensity was negatively correlated with maximum temperature, RH II and evaporation which were significant while minimum temperature and RH I were non-significant. Sunshine hours and wind speed were positively correlated and non significant. Multiple linear regression analysis revealed that maximum temperature and RH II significantly influenced the disease intensity while evaporation also showed partial influence on disease intensity.

Disease intensity was negatively correlated with temperature, rainfall, sunshine hours and wind speed; positively correlated with RH and evaporation during kharif 2015. Significant correlation was recorded with temperature; RH I and RF. Multiple linear regression analysis revealed that temperature, RH II and wind speed significantly influenced the disease intensity (Table-23a).

Studies on the impact of weather factors on the intensity of foliar and soil borne diseases starting from 35th and 40th meteorological week at TNAU, Coimbatore revealed that foliar diseases such as Alternaria leaf blight was found to be associated with varieties MCU13 and hybrids viz, RCHBGII and TCH32. However, comparison between different meteorological weeks indicated that, the incidence of foliar diseases was found to be initiated during 35th meteorological week (September, 2015). The maximum incidence of Alternaria leaf blight (8.0PDI) was observed in the hybrids viz, RCHBGII, TCH32 and variety. The maximum incidence of Alternaria leaf blight was observed during peak boll bursting stage. The incidence of TSV was found to be associated up to 9.0% in RGHBGII and TCH32 followed by 7.0% in the variety MCU13. Similarly, assessment of root rot revealed that the maximum incidence of 15.0% was observed during 45th meteorological week in the variety MCU13 and disease incidence was not observed in hybrids. However, in general the incidence of TSV and leaf spot were found to be more in the hybrid than the variety (Table-24).

1(c): Studies on the variability of Alternaria leaf spot (all centers)

Nanded center has reported Alternaria dianthi in isolations from their samples. At Junagarh center samples were collected from seven different location and pure cultures were obtained (Table-25). At Dharwad center, Among the Six isolates of Alternaria isolated from the different infected samples during 2015-16, four isolates (from Kalghatagi, Murgod, Hebballi farm, and Gadag) resemble to Alternaria macrospora with respect to length of conidia, beak length and number of septa. The other two isolates differed from Alternaria macrospora with respect to the conidial characters (Table-26). During the survey for cotton diseases at Guntur, Alternaria leaf spot samples were collected from 16 locations in farmers’ fields and experimental plots at RARS, Lam and observed for the presence of Alternaria (Table-27). Based on the spore structure the species was identified as Alternaria macrospora. The fungus was cultured and the isolate was sent to TNAU, Coimbatore for molecular analysis. At TNAU, Coimbatore, 25 isolates of Alternaria were obtained and their symptoms and culture characteristics were documented. (Table-28).

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 9 1(d): Survey and Epidemiology of TSV (Guntur, Nandyal, TNAU Coimbatore, Akola, Nanded & Rahuri)

The occurrence of Tobacco streak virus (TSV) was observed from first fortnight of July on most of the Bt cotton hybrids sown on farmers field around Rahuri, Maharashtra. However, the incidence of the disease was only up to five per cent during the season. Moreover, the virus was observed to infect only the upper leaves and symptoms were confined to leaves only. Systemic infection of the virus was rarely noticed. As against, incidence of the disease during 2014-15 was up to 40%. Environmental conditions during the current year may be the reason for low incidence of the disease (Table-29).

Nanded center, Maharashtra surveyed six districts ie Aurangabad (Fulambri); Parbhani (Selu); Hingoli (Kalamanuri); Latur (Ahemadpur); Jalna (Ambad) of Marathwada region for TSV incidence and confirmation for TSV but not a single infected plant was observed.

During the surveys conducted in Cotton growing districts by Guntur center, Tobacco Streak Virus disease was recorded from September to December 2015 on Cotton. The incidence on different hybrids varied from 0% to 12% (Table-30). TSV disease incidence was negligible on cotton varieties and hybrids in experimental plots of Regional Agricultural Research Station, Lam during September 2015 January 2016. Progress of TSV disease incidence (%) was in the range of 0.8% to 13.0% in Bt Cotton hybrids (Table-30a). The incidence was more during September when the crop was at 5 to 6 weeks old and thrips counts were in the range of 0.0 to 2 per three leaves. The symptoms were restricted to few leaves and/or branches. The incidence of TSV disease was less from second fortnight of October onwards and thrips were not found.

Direct Antigen Coated Enzyme Linked Immunosorbent Assay of cotton leaves showing different symptoms confirmed the presence of TSV. Analysis of common weeds in cotton plots revealed the presence of TSV in Cyperus rotundus, Cleome viscose and Panicum species (Table- 30b).

Survey for the occurrence of viral diseases by TNAU, Coimbatore revealed that Tobacco streak virus disease incidence was observed in all cotton growing tracts surveyed in Tamil Nadu. Incidence of TSV varied from 1.0 to 11.0%. The incidence was maximum (11.0%) in the Coimbatore. The minimum incidence of 1.0 and 2.0% of TSV was noticed in the Dindigul, Palladam, Anthiyur and Dharmapuri. TSV disease incidence was more in rainy season when compared to other seasons (Table-31).

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 10 Path 2 (a): Screening of breeding lines for diseases reaction (all centers): Table 32-71

Path 2(b): Confirmation and maintenance of disease resistant lines (all centers)

The results (Table-72) showed that out of 24 entries tested under field conditions at Faridkot against cotton leaf curl disease (CLCuD), two entries viz., Su-Flum and Bihani-251 gave moderately resistant reaction and one entry namely, MR 68 moderately susceptible reaction and four entries F 2164, H 1471, SSGR105 and CSH-2931 gave susceptible reaction to (CLCuD) and all other entries gave highly susceptible reaction. Against bacterial blight five entries viz., Su-Flum, Bihani-251, F 2501, RS 2765 and MR 68 gave resistant reaction and all other entries gave moderately resistant reaction and RST 9 gave moderately susceptible reaction. Against fungal foliar leaf spot (FFLS) caused by Myrothecium, Alternaria and Cercospora, one entry viz Bihani-251 gave resistant reaction and RST 9 gave moderately susceptible reaction. All other entries were found in moderately resistant group.

In National trial (Br 02) entries namely RS 2670, L1008 and SCS 1001 were found highly resistant against CLCuD during 2011-2012 crop seasons. Above entries were sown further during 2012-13, 2013-14 and 2014-15 crop seasons for confirmation of resistance against CLCuD. But all the entries were found susceptible to CLCuD under unprotected natural conditions. Hence no resistant entry was available for confirmation of resistance against CLCuD at CCS HAU, Hisar centre.

Twenty one breeding lines from Central Zone and National trials showing resistant during 2014-15, were sown in earthen pots during 2015-16 and screened against Alternaria blight disease under in vivo conditions by artificial inoculation of fresh culture of A. macrospora at Rahuri. The susceptible check LRA-5166 was also sown for comparison. The reaction of breeding lines is given in Table .Twelve lines showed resistant reaction against ALB CCH 12-2; GTHV 04/13; GJHV 497; CNH 5; L 1008; GSB 45; ARBB 1302; ZC (Suvin); TCB 37; ARBD 27; DSC 1401; RAHC 1011(Table-73). For Bacterial Blight it was observed that during 2014-15 and 2015-16, among 12 genotypes tested, 3 genotypes showed resistance to bacterial blight i.e. (NDLH-1943, P-2151, ADB- 542) whereas remaining genotypes were categorized as moderately resistant i.e. (BHV 180, AKH 10-1, BS – 30, AKH – 8828, BGDS-1063, SCH-1062, BS – 79, BGHV – 180, SCS - 793).( Table-73a)

At Nanded, twenty four breeding lines screened during 2015-16 against important diseases viz., Alternaria Leaf spot, Bacterial blight, Grey mildew, TSV, Wilt and para wilt. From each line five plants were randomly selected and artificially sprayed by culture of Bacterial blight, and Alternaria leaf blight. None of the line was found disease free and resistant to cotton diseases.

At Akola, it was observed that during 2014-15 and 2015-16, among 12 genotypes tested, 3 genotypes were resistant to bacterial blight i.e. (NDLH-1943, P-2151, ADB- 542) whereas remaining genotypes were categorized as moderately resistant i.e. (BHV 180, AKH 10-1, BS – 30, AKH – 8828, BGDS-1063, SCH-1062, BS – 79, BGHV – 180, SCS - 793). (Table-74)

The entries found disease free and resistant during previous year were tested this year under natural field and artificial condition against the different diseases at Surat. Infester row of susceptible check LRA 5166 was grown alternating every two rows of the test entries. Artificial inoculation was made twice by preparing the spore suspension of BLB. Total 2 entries were screened through artificial inoculation, from these entries viz., GSHH 2729 and GSHV 162 was

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 11 observed disease free under the artificial reaction. Alternaria leaf spot disease was not observed (Table-75).

Seventeen breeding lines were evaluated against Alternaria leaf spot, bacterial leaf blight and grey mildew diseases under field condition during kharif-2015 at Junagadh. Two entries recorded susceptible, 15 moderately susceptible reactions against Alternaria leaf spot disease. In case of bacterial leaf blight disease, the sixteen entries found resistance and one entry found moderately resistance. The grey mildew disease was not observed during the Kharif-2015(Table- 76).

Five varieties viz., ARBB 1401, DB 1402, CCB 36, ARBB 1402 and DB 1302 along with two susceptible checks RCH 2 BG II (for bacterial blight and rust) and Jadoo BG II (for Alternaria leaf spot and grey mildew) were evaluated for second consecutive year at Guntur. Two checks RCH 2 BG II (for bacterial blight and rust) and Jadoo BG II (for Alternaria leaf spot and grey mildew) after every four rows of randomized entries and also as border. Natural disease pressure was supplemented with artificial inoculations with grey mildew spore suspensions. The test entries showed resistant reaction to grey mildew disease and moderately resistant reaction to Alternaria leaf spot while the respective check entries showed susceptible reaction (Table-77).

The genotype SVA-1118 exhibited resistance to Alternaria blight, Bacterial blight, Grey mildew and Rust at Dharwad. The genotypes DB-14, DB-40 and SCS-1206 showed resistant reaction against Alternaria blight, Bacterial blight and Grey mildew but found susceptible against Rust (Table-78).

At Coimbatore, Seventeen entries from Br03a, Br04a and Br06a zonal trials of 2014-15 were screened against foliar diseases under natural conditions. The entries viz., ZC (Suraj), TSH04/115, Local Check, GJHV-516, CCH13-2, BGDS1063, DSC-1302 and DSC-1301 were free from Myrothecium leaf spot, root rot, grey mildew and BLB (Table-79).

Path 2(c): Monitoring of breakdown of resistance against CLCuD in cotton(Centers-Hisar, Sriganaganagar & Bhatinda).

A total of eight cultures (three CLCuD susceptible and three resistant G hirsutum varieties along with one susceptible and tolerant Bt hybrid) were screened against CLCuD at three locations to monitor break down of resistance due to appearance of new recombinant strains at 3 locations. The cumulative reaction results revealed that all the four susceptible cultures showed HS reaction whereas the three resistant cultures also showed HS reaction. The tolerant Bt hybrid showed susceptible reaction (Table-80).

Path 3 (a1): Validation of seed dressing chemicals against seed and soil borne diseases of cotton (Centers - Junagarh, Dharwad, Guntur & Coimbatore).

Based on pooled results of three years (2012-14), the minimum seedling mortality was observed when seed was treated with Carboxin 37.5%+ Thiram 37.5%DS at 4.5 g /kg followed by Thiram 75 WP at 4g/kg and Carboxin 75 % at 3g/kg. In case of Alternaria blight also the minimum PDI was observed when seed was treated with Carboxin 37.5%+ Thiram 37.5%DS at 4.5 g /kg but this was followed by Carboxin 37.5%+ Thiram 37.5%DS at 3.5 g /kg and Carboxin at 3g/kg. Bacterial

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 12 blight was best controlled by Thiram75WS at 4g/kg followed by Thiram75WS at 3g/kg & Carboxin 75 % at 2g/kg. Root was observed minimum in case of seed treatment of Carboxin 37.5%+ Thiram 37.5%DS at 4.5 g /kg followed by Thiram 75 WP at 4g/kg & Carboxin 37.5%+ Thiram 37.5%DS at 3.5 g /kg. Maximum seed cotton yield also was noted in Carboxin 37.5%+ Thiram 37.5%DS at 4.5 g /kg treatment (10.79 q/ha) followed by Carboxin 37.5%+ Thiram 37.5%DS at 3.5 g /kg & Thiram 75 WP at 4g/kg. Maximum benefit cost ratio was noted in treatment Carboxin 37.5%+ Thiram 37.5%DS at 4.5 g /kg at Dharwad and Coimbatore (Table-81).

Path 3 (a3): Evaluation of TrichoCASH (Trichoderma harzianum) CICR-G 1% WP for cotton root diseases at CICR Sirsa and Pune

The trichocash (Trichoderma harzianum) CICR-G 1% WP (seed treatment @ 5 & 10 g/kg seed alone and with drenching @ 0.5% & 1.0%) evaluation alone and in combination with thiram seed treatment(@3g/kg seed) was done against root rot disease of cotton caused by Rhizoctonia sps at CICR, regional station Sirsa under sick field conditions. Non significant results were obtained during the season. However, numerically lowest seedling mortality was noted in trichocash treatment @10g/kg seed + Thiram @3g/kg seed followed by trichocash treatment @5g/kg seed. In case of root rot observations, lowest incidence was noted in commercial Trichoderma formulation seed treatment @10g/kg seed followed by trichocash treatment @5g/kg seed (Table-82)

At Pune center, TrichoCASH) @ 10g/kg seed + Thiram @ 3g/kg showed maximum 45.26% disease control of cotton wilt, followed by Tricho CASH) @ 5g/kg seed + Thiram @ 3g/kg (43.33%) disease control (Table-83).

Path 3 (c): Developing IDM modules for the management of cotton diseases (Centres- Dharwad, Akola, Junagarh)

The maximum bacterial blight disease intensity 8.3 per cent was recorded on Bunny Bt in the Control plot (untreated plot) at Akola. All six different modules were found effective in managing the bacterial blight disease of cotton over untreated plot, where neither seed treatment nor any fungicide/ bioagent sprays were given (Table-84). However, the seed treatments of bioagent with chemical sprays were found more effective in minimizing the BLB disease in Bt hybrid (module 5).The significantly maximum seed cotton yield (13.5 q/ha) compared to control was recorded in the treatment of Bunny Bt + module 3 which was at par with treatment Bunny Bt + module 5 (13.03 q/ha) and Bunny Bt + module 2. Whereas, the minimum seed cotton yield 8.4 q/ha was recorded in untreated plot. The results on yield contributing parameters revealed that there was not much difference in seed germination (92.8 to 97.9 %), days to flowering (38 to 42 days) and root length (37.2 to 41.2 cm) due to different treatments. There was not much variation in plant height in Bt hybrid with different module, however plant height of Bunny Bt was (64.1 to 71.1 cm) whereas, the highest plant height 71.1 cm was recorded in the treatment of soil application of Pseudomonas fluorescens (PF - TNAU) @ 2.5 Kg/ha Foliar spray with P. fluorescens (module 3), which was followed by Seed Treatment of Pseudomonas fluorescens PF CICR @10 g/Kg of seed; Soil Application of Trichoderma viride @ 2.5 Kg /ha TV-TNAU1 FS with Propiconazol 0.1% for foliar diseases and COC (0.3%) + Streptocycline (0.01%) for BLB or Carbendazim 0.1% for grey mildew on need basis (module 5).

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 13 At Junagadh center, out of the total seven Modules including the control, Module-6 (8.8%) followed by Module-5 (9.2%) and Module-1(9.8%) recorded significantly minimum Alternaria leaf spot as compared to the Module-7 i.e. Control (16.4%) in RCH-2 BG-II hybrid, while all the remaining Modules found statistical at par with the Module -7 i.e. control for Alternaria leaf spot. For bacterial leaf blight, all the Modules except Module- 4 recorded significantly minimum bacterial leaf blight in RCH-2 BG-II hybrid as compared to the control. Out of total Modules used in field condition the minimum bacterial leaf blight was recorded in Module-6 (1.8%), followed by Module- 5 (1.97%), Module-3 (2.2%), Module-2 (2.3%) and Module-1 (2.5%) as compared to the control i.e. (3.99%). Considering mortality per cent, the minimum mortality per cent (2.9%) was recorded in Module-6 and it was at par with rest of Modules except control. The highest seed cotton yield was recorded in Module-6 (18.3 Q/ha), followed by Module-5 (17.5Q/ha) and Module-2 (17.1 Q/ha). The seed cotton yield was found statistically significant in Module-6 and it was at par with Module- 5 and Module-2 (Table85). The highest mean germination percentage was recorded in Module-6 (99.3%) and the lowest mean germination percentage was recorded in control (93.0%). The same results were also found for plant height in RCH-2 BG-II hybrid at Junagadh centre (Table-85a).

At Dharwad, least per cent of seedling mortality was found in T6 (Pseudomonas fluorescens (PF-CICR) @ 10g/kg of seed Soil application of Trichoderma viride @ 2.5 kg/ha (TV-TNAU) in 250 kg of Compost or FYM foliar spray with Ergon @ 1ml/lit followed by Taqat @ 1.5g/lit for Fungal diseases or COC 0.3% + Streptocycline 0.01% for BLB). With respect to per cent disease index, T6 recorded lower incidence with higher yields. Though higher yield was recorded in T6 it was found to be on par with T4 (Table-86).

Path 3 (e): Innovative interventions for the management of CLCuD(Centers-Hisar, Faridkot, Sriganganagar, CICR, Sirsa and Bhatinda).

The experiment conducted at different locations to study the efficacy of ecological interventions i.e., Butter milk @ 5%, Cow urine @6.6%, Neem oil@1%, Mustard oil@3%, Calcium [email protected]%, Cow urine + Calcium nitrate, Cow urine + Butter milk, Butter milk + Calcium nitrate, Lachesis30@1ml/l, Digitalis@1ml/l, Apis mallifera30@1ml/l, Bryonia30@1ml/l, Natrum Mure @1ml/l, [email protected]%, and control was studied during 2015-16.The minimum CLCuD PDI was noted in Polo spray @0.1% followed by cow urine @5%, neem oil and cow urine + calcium nitrate combination. (Table 87). Maximum seed cotton yield was also observed in polo spray followed by cow urine +calcium nitrate treatment (Table 87a).As per the reduction in white fly population during the peak population period at all the locations, in case of Sirsa maximum reduction was obtained under Polo (36.75%) followed by cow urine (15.98%), neem oil and mustard oil. In Hisar Polo, Buttermilk (12.28%), Naturum mure (12.84%) and Bryonia (11.30%) comparatively reduced the population of whitefly. In Sriganganagar, Neem oil (50.41%) followed by polo (47%) gave the maximum reduction however in all the treatments a good reduction in whitefly population (34.29- 48.81%) was recorded. (Tables 87 b,c &d)

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 14 Path 4(e): Crop loss estimation due to CLCuD and distribution pattern of CLCuD in North zone.

Experiment-1: To work out relationship between disease index and yield reduction due to cotton leaf curl virus disease (Centers-Hisar, Faridkot & Sriganganagar).

The percent seed cotton yield reduction ranged from 10.3 – 26.0, 28.6-37.6 and 17.3 -34.8 respectively in Bt cotton hybrids(Bioseed 6588 BG II, RCH 650 BG II, Ankur 3028 BG II & MRC 7017 BG II) tested at Hisar, Faridkot and Sriganganagar locations respectively (Table-88).In case of grade wise seed cotton yield reduction (%) 34.1-72.5 and 5.1 81.3 in Bioseed 6588 ;33.5-56.7 and 6.3 to 67.5 in RCH 650 ; 39.7-68.7 and 12.0 to 64.5 in Ankur 3028 ; 28.3-55.7 and 12.9-66.3 in MRC 7017 respectively at Hisar and Faridkot was observed (Table-88a).

Experiment-2: Study on distribution pattern of cotton leaf curl virus disease on local popular Bt hybrid at farmer’s field (Centers: Hisar, Faridkot & Sriganganagar). The district wise pooled CLCuD PDI data of Punjab showed that per cent disease index varied from 34.0-45.3.Rajasthan’s Sriganganagar district showed a per cent disease index of 14.8. The district wise pooled CLCuD PDI data of Haryana showed that per cent disease index varied from 8.6-34.4.No CLCuD was observed at farmer’s field in Bhiwani (Siwani & Loharu blocks), Rewari, Mahendergarh, Palwal and Jhajjar districts (Tables-89).

Experiment-3:CLCuD progress and yield estimations (CICR Sirsa center). In order to study CLCuD progress and yield estimations, an experiment with two resistant and two susceptible Bt hybrids was conducted at Sirsa in half an acre area. The disease progress (%incidence) in resistant hybrid Yuva and Bunty from 50DAS to 140 DAS was 0-4.2 and 0-14.11 respectively. Whereas it was 2.08-48.68 and 7.0 – 57 08 in susceptible hybrids Ankur Jai Bt and Ankur 3028 respectively (Table 90).The PDI in above referred hybrids from 80 DAS to 140 DAS ranged from 0.01 - 1.4; 0.6 - 4.8; 5.4 - 22.7 and 9.0 - 30.9, respectively. Seed cotton yield in Yuva, Bunty, Ankur Jai BT and Ankur 3028 was 6.9, 9.2, 8.3 and 9.2 q/ha, respectively (Table 90a).

Path 7: Fusarium wilt of cotton (Pune centre)

Total 169 genotypes received from the Project Coordinator, (Cotton Improvement) & Head CICR Coimbatore, Principal Scientist and I/C Oilseed Research Station Jalgaon, Cotton Breeder, were tested for Seedling Resistance (SRT) in glass house. All the entries showed susceptible reaction against Fusarium wilt.

Sixteen Intra hirsutum and 12 G. hirsutum x barbadense hybrids received from Cotton Breeder, MPKV, Rahuri were found free from Fusarium infection at seedlings stage. The root rot infection ranged from 14.81-61.54% in different entries was noticed in genotypes tested during 2015. The lowest Rhizoctoina root rot was observed in the entry RHB-14.81 (14.81) followed by Phule 328 (15.38) under pot sick soil condition. The incidence of Tobacco Streak Virus was recorded 0.00to 2.86 % in G. hirsutum. The entries RHH-0917, RHH-0707, RHH-1007, RHH-1015, RHH-1112 and RHH-1025 remain free from infection. In G. barbadense 0.00 to 2.33%TSV was recorded. The entries RHB-0708, RHB-0707, RHB-0922, RHB-0711 and RHB-1123 were free from TSV infection.

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 15 Path 1(a): Observations on the occurrence of various diseases (in farmer’s field and research farms) NORTH ZONE Table 1: Observations on the occurrence of various diseases in farmer’s field and research farms (Faridkot) CLCuD BB FFLS District Locations Variety/Hybrid Acerage (PDI) (DI) (DI) 79 1.2 1.8 F 2228 71 1.5 1.4 F 2383 66 0 Traces F 2164 72 1.2 1.9 F 1054 68 1.2 1.9 F 1378 R.S. Faridkot 66 1.3 1.7 F 505 74 1.9 1.8 F 846 62 1.9 1.7 F 1861 0.1- 0.1- Different entries of Co-ordinated and State 0.3-2.6 Faridkot .89.2 2.9 trials Sindwan 40 1 2-Jan Bioseed 6488 Bt, 6acre Bioseed 6488 Bt 1acre Romana 57 Traces 2-Jan Bioseed 6588Bt 1acre 66 2.1 3-Jan JK 1947 Bt 3 acre Kaliana 58 1.8 2-Jan Bioseed 6588 Bt 3acre 47 Nil 0.1 – 2 RCH 650 Bt 2 acre Chahal 49 Traces Traces RCH 773 Bt 2 acre Saddiq 57 0.8 2 Bioseed 6488 Bt 5 acre Kotkapura 43 1.1 1.2 Bioseed 6588 Bt 7 acre 77 1 1.7 F 2228 65 1.1 1.1 F 2383 66 1.2 1.9 F 505 CRS Abohar 62 1.6 2.4 F 846

68 1.1 1.7 F 1861 61.4 0.8 1.1 F 1378 66 0.1 1.1 F 1054 65 1.5 1.5 Bioseed 6488 Bt 5 acre Zandwala khatta 70 Traces 0.1 Bioseed 6588 Bt 5 acre Fazilka Khuiya 75 1.8 1.5 Bioseed 6488 Bt - Khera 65 1.9 Traces Bioseed 6588Bt 40 0.7 1.5 Bioseed 6588 Bt 2 acre Alamgarh 47 0.1 1.1 Bioseed 6488 Bt 2acre 45 Traces Traces Bioseed 6588 Bt 3 acre Kamalwara 49 Traces Traces Bioseed 6488 Bt 3 acre 0.1- 65 2-Jan Bioseed 6488 Bt 5 acre Gobindgarh 2.0 65 2-Jan Traces Bioseed 6588Bt 5acre Kundal 60 2-Jan Traces Bioseed 6588Bt 4 acre 65 0.3-1 0.1-2.1 Bioseed 6488 Bt 10 acre 1.1-1.5 55 2-Jan Bioseed 6588 Bt 2acre Mallan Traces 47 Traces Traces RCH 773 Bt 2acre 37 Traces RCH 650 Bt 2 acre 44 Traces 2-Jan RCH 773 Bt 7 acre Baang 40 Traces 2-Jan RCH 653 Bt 2 acre Muktsar 55 Traces Traces JK 109 Bt 2 acre Kothe Dashmesh 40 2-Jan 2-Jan RCH 773 Bt 4 acre nagar 52 2-Jan Traces Ankur 3028 Bt 5 acre Muktsar 46 2-Jan Traces Ankur 3244Bt 5 acre Maha Bhaddar 40 2-Jan 2-Jan RCH 651Bt 2.5 acre Kotbhai 44 2-Jan Traces Bioseed 6588 Bt 2 acre

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 16

Table 2: Observations on the occurrence of various diseases in farmer’s field and research farms (Bathinda) Bacterial Fungal Foliar Leaf CLCuD Root rot Village Block District Hybrid blight Spots (%) (PDI) (Grade) (Grade) RCH 650 27.33 0 0 0 Katar Singh Wala Ankur 3028 46.67 0 0 0 Jeon Singh Wala MRC 7017 52 0 0.0-1.0 10% RCH 776 49.33 0.0-1.0 0 0 Sukha Singh Wala Bioseed 60.67 0 0 0 6588 Bathinda Bathinda RCH 653 34 0 0.0-1.0 0 Kothepule Singh Bioseed Wala 52 0 0.0-1.0 0 6488 RCH 653 38 0 0 0 Ankur 3028 51.33 0 0 0 Jodhpur NCS 855 41.33 0 0 0 US 21 72.67 1.1-2.0 0.0-1.0 10% RCH 653 28 0 0 0 RCH 773 44 0.0-1.0 0.0-1.0 0 Ankur 3224 59.33 0 0.0-1.0 0 Gehri Buttar Bioseed 53.33 0 0 0 6588 RCH 776 54 0.0-1.0 0 0 RCH 773 45.33 0 0.0-1.0 0 Naruana RCH 653 30 0 0.0-1.0 0 RCH 653 33.33 0 0 0 Bioseed Phullomithi 58 0 0 0 Sangat Bathinda 6588 Ankur 3028 52 0 0 0 Gurthari RCH 650 30.67 0 0 0 RCH 653 32.67 0 0 0 Sangat Kalan RCH 773 42.67 0.0-1.0 0.0-1.0 0 MRC 7017 45.33 0 0 0 Machhana MRC 7017 40.67 0 0 0 Sangat Mandi RCH 650 25.33 0 0.0-1.0 0 RCH 653 29.33 0 0 0 Gehri Buttar RCH 773 51.33 0.0-1.0 0.0-1.0 0 RCH 776 46.67 0.0-1.0 0.0-1.0 0 Zateri Maur Bathinda RCH 650 26.67 0 0 0 Bioseed 64.67 0 0.0-1.0 0 6488 Bioseed Talwandi 64 0 0 0 Kot shameer Bathinda 6588 Sabo Ankur 3028 43.33 0 0 0 NCS 855 39.33 0.0-1.0 0 0 RCH 650 28.67 0 0 0 Bioseed Jhanda Kalan 51.33 0 0 0 Mansa Mansa 6488 Birpur Kalan RCH 650 16.67 0 0 0 Patui Khera Lambi MRC 7017 42.67 0 0 0 Bioseed 56.67 0 0 0 Chotia Mukstar 6488 Giddarba NCS 855 35.33 0 0 0 Theri RCH 653 31.33 0 0 0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 17

Table 3: Observations on the occurrence of various diseases in farmer’s field and research farms (Sriganganagar)

Disease Tehsil Area surveyed Bt hybrids/varieties PDI range Sriganganagar district 3Y, 9Y, Kaliyan, Khatlabana, Shivpur head, Sadhuwali, Bioseed 6588 Sriganganagar Sujawalpur, Hindumalkot, 31.1 13.3-41.7 Bioseed 6488 Kotha, Pakki, Mirzewala, MRCH 7017 Daulatpura NCS 855 Kaminpura, 39 GG, 48GG, Srikaranpur Ankur 3028 5.2 5.0-12.4 8NN, Delwa, Kureshian Bihani 161 Rattewala, Guddhuwala, 14 Padampur RCH 650 2.7 0-7.3 BB, 18BB, Sawantsar JKCH 1947 14KSR, Manaksar, Paliwala, Suratgarh 0 0 Lalpura District average 9.7 0-41.7 Hanumangarh district Bioseed 6588 Sriram 6588 MRCH 7017 NCS 855 Pakka saharana, NCS 9013 Chohillawali, Jorkian, Ankur 3028 6.1 3.9-14.6 Chistiyan, Ayalki, Uttamsingh Ankur 3244 wala, Karnisar JKCH 1947 JKCH 0109 Bihani 161 RCH 650 District average 6.1 3.9-14.6

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 18 Table 4: Observations on the occurrence of various diseases in farmer’s field and research farms (Hisar) District Cotton August September Village Hybrid CLCuD BLB MLS ALS RR CLCuD BLB MLS ALS RR Babalpur RCH 650 BGII 33.3 0.0 9.5 0.0 0.0 50.0 0.0 20.3 0.0 0.0 Dhani Prem RCH 602 BGII 3.3 0.0 10.8 0.0 0.0 13.3 0.0 25.0 0.0 0.0 Nagar Jewra RCH 650 BGII 20.0 0.0 5.5 0.0 0.0 30.0 0.0 15.0 0.0 0.0 Balak Bio 6488 BGII 15.0 0.0 7.8 0.0 0.0 21.7 0.0 19.3 0.0 0.0 Kirori Bio 100 BGII 1.7 0.0 5.5 0.0 0.0 13.3 0.0 11.5 0.0 0.0 Bioseed 6488 Sanyana 5.0 0.0 8.5 0.0 0.0 23.3 0.0 21.3 0.0 0.0 BGII Mehnda RCH 650 BG-II 20.0 0.0 11.5 0.0 0.0 50.0 0.0 23.8 0.0 0.0 Garhi

Ankur 3028 Siswal 16.7 0.0 16.3 0.0 0.0 58.3 0.0 25.0 0.0 0.0 BGII

HS-6 29.4 0.0 5.4 0.0 0.0 66.4 0.0 12.2 0.0 0.0

NCS 855 32.7 0.0 1.2 0.0 0.0 66.6 0.0 14.0 0.0 0.0 Hisar RCH 650 18.6 0.0 7.0 0.0 0.0 49.9 0.0 10.0 0.0 0.0 Bio-6588 BG 22.3 0.0 1.4 0.0 0.0 58.3 0.0 11.5 0.0 0.0 II HAU, BIO 6488 BG Research 16.2 0.0 5.6 0.0 0.0 49.9 0.0 20.5 0.0 0.0 II Farm BIO 100 BG II 1.4 0.0 6.0 0.0 0.0 4.8 0.0 10.4 0.0 0.0 RCH 653 BG II 19.9 0.0 2.2 0.0 0.0 58.3 0.0 8.5 0.0 0.0 Ankur 3028 35.1 0.0 6.2 0.0 0.0 66.6 0.0 16.6 0.0 0.0 BG II Bunty 12.0 0.0 5.4 0.0 0.0 33.3 0.0 12.3 0.0 0.0 Bioseed 6588 Daryapur 3.3 0.0 13.5 0.0 0.0 13.3 0.0 25.0 0.0 0.0 BGII Badopal RCH 650 BGII 1.7 0.0 10.5 0.0 0.0 13.3 0.0 21.8 0.0 0.0 Khara Kheri Bio 105 BG II 6.7 0.0 11.8 0.0 0.0 26.7 0.0 23.5 0.0 0.0 Dhingsara RCH 602 BGII 23.3 0.0 11.0 0.0 0.0 46.6 0.0 21.8 0.0 0.0 Ankur 3028 Dhabi Kalan 8.3 0.0 8.3 0.0 0.0 33.3 0.0 13.5 0.0 0.0 BGII Fatehabad Jhandi Khurd RCH 650 BG-II 13.3 0.0 0.0 0.0 0.0 41.7 0.0 11.0 0.0 0.0 Bhuna RCH 773 BG II 33.3 0.0 3.8 0.0 0.0 36.7 0.0 16.5 0.0 0.0 Jhalania RCH 602 BGII 8.3 0.0 6.5 0.0 0.0 43.3 0.0 22.0 0.0 0.0 Jandli Kalan RCH 602 BGII 25.0 0.0 0.0 0.0 0.0 28.3 0.0 8.8 0.0 0.0 Panjuwana RCH 650 BG II 1.7 0.0 4.0 0.0 0.0 21.7 0.0 16.8 0.0 0.0

Darba Kalan RCH 650 BG II 5.0 0.0 10.0 0.0 0.0 43.3 0.0 24.5 0.0 0.0 Sirsa Odhan RCH 602 BGII 13.3 0.0 5.8 0.0 0.0 38.3 0.0 16.3 0.0 0.0 Jajjanwala Raghav 1.7 0.0 14.3 0.0 0.0 21.7 0.0 25.0 0.0 0.0 Dumer Khan RCH 650 BGII 3.3 0.0 5.8 0.0 0.0 28.3 0.0 20.0 0.0 0.0 Khatkar Bio 6588 BGII 3.3 0.0 0.0 0.0 0.0 25.0 0.0 11.0 0.0 0.0 Baroda Xylem 3.3 0.0 0.0 0.0 0.0 16.7 0.0 9.3 0.0 0.0 Jind Sindhvi Balwan 3.3 0.0 16.3 0.0 0.0 41.7 0.0 25.0 0.0 0.0 Khera Danoda Bio 6588 30.0 0.0 11.8 0.0 0.0 50.0 0.0 24.3 0.0 0.0 Badesera RCH 653 BGII 0.0 0.0 0.0 0.0 0.0 20.0 0.0 6.3 0.0 0.0 Mandola Bio 6588 BGII 0.0 0.0 0.0 0.0 0.0 21.7 0.0 8.0 0.0 0.0

Obera RCH 602 BGII 0.0 0.0 0.0 0.0 0.0 0.0 0.0 7.5 0.0 0.0 Bhiwani Miran Bio 6588 BG II 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Sangwan RCH 650 BG II 0.0 0.0 0.0 0.0 0.0 21.7 0.0 0.0 0.0 0.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 19 CENTRAL ZONE

Table 5: Observations on the occurrence of various diseases in farmers’ field and research farms (Rahuri) % Disease Intensity/Incidence Sr. No. Locations Variety / Hybrid ALB TSV% LRA-5166 20.70 2.51 Phule 688 9.85 -- Phule 388 10.37 2.00 Phule 492 12.28 -- RHC 717 5.90 -- Kanak 954 12.20 1.53 1. Cotton Improvement Project, MPKV, Rahuri NCS 245 (Bhakti) 6.42 1.74 Ajit 155 5.10 1.21 Ajit 199 5.28 1.42 MRC 7351 13.25 3.54 RHcB -011 6.62 -- Phule Dhanvantari 4.25 -- Phule Anmol 5.22 -- Farmers field Locations Variety/hybrids PDI (ALB) Bhakti 14.06 Ajit 199 7.82 Rahuri Ajit 155 9.05

MRC 7351 13.57 Jadoo(Kaveri) 10.12 Ajit 199 6.05 Pathardi Ajit 155 8.34

Chamatkar 9.22 Mallika Bt 14.15 MRC 7351 15.09 Shevgaon Ajeet 199 5.23

Chamatkar 10.11 NCH 954 12.05 Chanakya 10.27 Sangmner Bt No. 228 13.15 Ajit 199 8.05 Surbhi 4.21 Sangola Phule 388 3.22 RHB 0711 3.15 Bt 992 2.15 Super fiber 2.27 Kavthemahankal Gold Kavita 3.42 Hira Daftari 7.50 Rashi 2 Bt 13.12 Jadoo(Kaveri) 12.15 Dhule Trinetra 19.13 Mallika 23.04 MRC 7351 14.56 MRC 7351 12.14 NCS 245(Bhakti) 13.17 Jalgaon Jadoo (Kaveri) 16.25 Ajit 199 8.07 Chamatkar 19.08 MRC 7347 14.17 Ajit 199 12.00 Nandurbar MRC 7351 19.70 Mallika 14.23 Pancham 9.03

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 20

Table 6: Observations on the occurrence of various diseases in farmer’s field and research farms (Nanded)

Bacterial Alternaria Grey-mildew *Para Wilt Varieties / hybrids blight (PDI) (PDI) (PDI) (PDI) 1 2 3 4 5 Mallika (Bt) 5.75 9.25 0.50 0.00 Bunny (Bt) 5.00 10.50 1.75 0.00 Ajeet 155 (Bt) 3.20 7.75 1.50 3.00 Dr. Brent (Bt) 3.00 7.59 0.00 0.00 Ankur (Bt) 3.20 8.00 1.00 0.00 RCH II (Bt) 7.00 13.00 2.50 0.00 Bramha (Bt) 5.00 10.15 1.50 0.00 NHH 250 (Non Bt) 3.50 11.00 1.50 0.00 NHH 206 (Non Bt) 4.00 11.25 1.50 0.00 LRA 5166 (Non Bt) (Ch) 8.50 21.50 3.00 0.00 PA 255 (G.arboreum) 3.50 6.50 0.00 0.00 PKV 081 (Non Bt) 5.00 15.20 1.50 0.00 Suraj (Non Bt) 4.25 13.50 1.00 0.00 NH 615(Non Bt) 3.50 12.00 1.00 0.00 NH 635 (Non Bt) 3.00 11.50 0.50 0.00 NH 545 (Non Bt) 3.00 10.50 0.50 0.00 GBHV 180 (Non Bt) 2.75 9.25 1.00 0.00 First Class 2.50 10.50 0.00 5.00 DHH 1251 (Non Bt) 2.50 8.00 1.00 0.00

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 21

Table 7: Observations on the occurrence of various diseases in farmer’s field and research farms (Akola)

Crop growth Stages Percent Disease Intensity (PDI) Variety/ District/ Place Seedling Flowering Boll development Hybrid (20-30 DAS) (60-80 DAS) (120-150 DAS) BLB MLS GM BLB MLS GM BLB MLS GM Akola district Hiwarkhed Ajeet 155 - - - 3.6 - - 3.5 - Dr.Brent - - - 2.75 - - 3.11 - AKH- 081 - - - 1.75 1.48 - 4.65 - Saundala Mallika-207 - - - 2.60 - - 5.41 - Vyala Ajeet 155 - - - 1.75 - - 4.58 - Jaadu - - - 1.90 0.94 - 3.65 - Barshi Takli Bhakti - - - 1.33 - - 2.37 - AKH-081 - - - 2.58 Traces - 3.51 - Raundala Paras Brahma - - - 1.72 - - 4.21 - Shivapur Rashi - - - 2.02 - - 4.9 - Ajeet155 - - - 1.56 Traces - 3.6 - Mallika - - - 3.75 - - 3.8 - Sonori Dr.Brent - - - 3.16 0.94 - 4.1 - Ajeet-155 - - - 4.08 1.03 - 4.16 - Babhulgaon Mallika-207 - - - 2.02 2.02 - 3.3 - Paras Bramha - - - 1.14 1.83 - 3.4 - Ugwa Chaitannya - - - 1.74 1.36 - 2.5 - Amravati Achalpur Ajeet 155 1.62 - - 1.8 - Anjangaon Dr.Brent 2.1 0.83 -- 2.6 - Sategaon Ajeet 155 2.01 - - 2.7 - BuldhanaDistrict - Malkapur First class - - - 1.75 Traces - 2.41 - Motala First class - - - 2.41 0.81 - 3.3 - Tulsi - - - 1.01 Traces - 2.2 - Research field Akola AKH-081 - - - 1.90 1.72 - 3.00 - AKH-8828 - - - 1.33 1.2 - 2.78 - PKV Rajat - - - 0.33 1.67 - 3.06 - AKH-9916 - - - 1.72 1.12 - 4.26 - AKA-5 - - - 2.56 1.02 - 3.26 - AKA-7 - - - 1.33 0.75 - 3.32 - AKA-8 - - - 2.16 0.72 - 3.12 - AKH-8401 - - - 1.67 ---- - 5.54 -

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 22

Table 8: Observations on the occurrence of various diseases in farmer’s field and research farms (Surat) Sr. Location/ Variety/ Disease (PDI) District Taluka Month No. Village Hybrid BLB ALS G. Cot. Hy.6 BG II 0 - 1.5 0 - 1 G. Cot. Hy.8 BG II 0 - 2.0 0 - 1 September - Surat MCRS LRA 5166 2.5 – 23.0 1 - 3 1 October Surat Suraj 3.5 - 12.5 0 – 4.5

G. Cot. Hy.12 2.0 – 23.5 0 - 1 Chandaniya Ajeet 155 BG II 1.5 – 5.5 0 - 2 Mangrol September Vasrayi Vikram 5 BG II 0 – 1.5 0 - 1 RCH-2 BG II 0 - 2 0 Valia Sodgam September Nuzuvedu-199 0 – 2.5 0 Nath-155 0 - 2 0 2 Bharuch RCH-2 BG II 0 – 1.9 0 Valia Tuna September Ankur - 3028 1 – 3.0 0 Bharuch Kelikuva September Ajeet BG II 1 – 5.5 0 – 2.5 Ajeet BG II 15 – 24.0 5-13.5 Nandod Kuta Amba September RCH-2 BG II 5.0 – 12.5 0 3 Narmada Rajpipla Chitrawadi September RCH-2 BG II 0 – 5.0 0 Netrang Kambodia September Ajeet BG II 1 – 8.0 0

Table 8(a): Observations on the occurrence of various diseases under high density planting system at Surat

Bacterial leaf blight Alternaria leaf spot Sr. Entries/ varieties Max Max No. PDI Reaction PDI Reaction Grade Grade 1 G. Cot. 16 8.5 2 MR 0.0 0 DF 2 GBHV 164 5.5 2 MR 0.0 0 DF 3 GBHV 186 4.0 2 MR 0.0 0 DF 4 GISV 272 3.5 2 MR 0.0 0 DF

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 23 Table 9: Observations on the occurrence of various diseases in farmer’s field and research farms (Junagadh) Per cent disease Percent Sr. Name of Village Taluka District Variety/hybrids/Bt incidence (%) Dis. No. Farmer ALS BLB GM RR Wilt Ramehs 1 Khamidana Kesod RCH-2 8 1 0 0 0 Jadavbhai Ashvin 2 Barvada Bhesan Ajit-155 BG II 10 2 0 0 0 Mansukh Jignesh 3 Ranapur Bhesan Badshah 16 1 0 0 1 Valabhai 4 Ajit Ratilal Bhalgam Manavder JK Varun BG-II 15 1.5 0 0 0 Harsed Junagadh 5 Vnthli Vnthli G.Cot.Hy-6 BG-II 10 3 0 0 0 Mohanbhai Mota 6 Nagji Dushabhai Bhesan Daymand 16 2 0 1 1 bhalgam 7 Kirit Samjibhai Umarakhi Bhesan G.Cot.Hy-6 BG-II 9 2 0 0 0 8 RamsiJeshabhai Shelda Vanthli Badshah 14 0 0 0 0 9 Parbat Valabhai Semarada Junagadh Ajit-155 BG II 17 0 0 0 0 10 Sanjay Fulabhai Avtadiya Bilkha JK Varun BG-II 10 0 0 0 0 Mahesh 11 Bagsra Bagasra Obama Krish 18 1 0 0 0 Batukray 12 Raghu Poptbhai Samervi Amreli Neno BG-II 14 2 0 0 0 Savarkundla 13 Balu Madha Vanot RCH-2 19 0 0 0 0 14 Sidharaj Bhikhu Anida Kukavav Ajit-199 11 2 0 0 0 Gopal 15 Kukada Mudi Surendra- RCH-2 14 1 0 0 0 Karsanbhai nagar 16 Harji Narsibhai Sangani Chotila Badshah 15 1 0 0 0 17 Manhar Babu Tarshai Jamjodhpur Jamnagar RCH-2 17 2 0 0 0 Dinesh Bhikha 18 Udakiya Krusi dhan 12 1 0 0 0 bhai Dhoraji Mansukh 19 Kalana Rajkot Mallika BG-II 15 1 0 0 0 Gokalbhai 20 Mahesh Ratilal Valpur Bagsra Ganesh BG-II 13 0 0 0 0 21 Amit Gangadash Nagvader Upaleta Maharaja gold 19 0 0 0 0 22 Viral Samgibhai Haripur Ajit-155 BG II 10 2.5 0 0 0 Lakhman 23 Chanduvav Gir Ajit-155 BG II 7 1 0 0 0 Virabhai Veraval Somnath Bhimsi 24 Supasi Ankur Jay BG-II 11 2 0 0 0 Meraman Viram 25 Valotra Ranavav Ankur -28 18 0 0 0 0 Bhagvanbhai 26 Mulji Ramjibhai Meradi Ranavav Porbandar Tulasi-4 9 0 0 0 1 Ramesh 27 Marvad Kutiyana RCH-2 18 1.5 0 0 0 Dhanjibhai Cotton Res. 28 G.Cot -6 16 4 0 0 0 Station Junagadh Junagadh Junagadh Cotton Res. Farm 29 G. Cot Hy-18 17 3 0 0 0 Station

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 24 SOUTH ZONE Table 10: Observations on the occurrence of various diseases in farmer’s field and research farms (Dharwad) PDI Locations Genotype Rainfed/ irrigated Crop Stage AB BB GM Rust Dharwad District Seedling stage 10 12 05 -- Yaadvada Dr. Brent Bt R Boll initiation 20 15 10 -- Boll Maturity 30 18 15 25 Seedling stage 12 05 05 -- Mulamuttala MRC-7351 Bt R Boll initiation 18 10 10 -- Boll Maturity 28 10 15 30 Seedling stage 10 08 10 -- Garag Arjun Bt R Boll initiation 15 10 10 -- Boll Maturity 25 20 20 25 Seedling stage 15 08 10 -- Amminabhavi MRC-7351 Bt R Boll initiation 18 12 15 -- Boll Maturity 25 18 15 35 Seedling stage 10 15 10 -- Tadkod First class Bt R Boll initiation 20 20 10 -- Boll Maturity 25 20 15 35 Seedling stage 08 10 05 -- UAS,Dharwad Chiranjeevi Bt R Boll initiation 18 15 10 -- Boll Maturity 25 25 20 30 Seedling stage 15 12 05 -- ARS, Dharwad Farm* Bunny Bt R Boll initiation 20 18 20 -- Boll Maturity 30 25 25 40 Seedling stage 12 05 05 -- Kalgatagi Taluk Arjun Bt R Boll initiation 15 10 10 -- Gambyapura Boll Maturity 28 15 15 35 Seedling stage 12 05 05 -- Kanavihonnapur First class Bt R Boll initiation 15 10 10 -- Boll Maturity 25 20 15 25 Seedling stage 15 10 10 -- Dummvada Arjun Bt R Boll initiation 20 20 15 -- Boll Maturity 25 25 20 30 Belgaum District . Seedling stage 05 10 10 -- Savdatti Dr. Brent Bt R Boll initiation 15 15 15 -- Boll Maturity 20 20 25 30 Seedling stage 15 10 10 -- Murgod RCH Bt R Boll initiation 20 20 15 -- Boll Maturity 25 20 15 35 Seedling stage 10 15 05 -- Malluar Chitannya Bt I Boll initiation 15 10 10 -- Boll Maturity 20 25 20 35 Seedling stage 15 10 10 -- Kolavi ATM Bt I Boll initiation 15 10 15 -- Boll Maturity 25 20 25 30 Haveri District Seedling stage 15 05 05 -- Haveri MRC-7351 Bt I Boll initiation 20 15 10 -- Boll Maturity 25 20 20 30 Seedling stage 12 10 10 -- Savanur Taluk Jakpot Bt R Boll initiation 15 15 15 -- Ichhangi Boll Maturity 28 20 20 20 Seedling stage 15 15 05 -- Hirekarur Taluk MRC-7351 Bt R Boll initiation 20 20 10 -- Yogikoppa Boll Maturity 28 25 15 35

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 25

Table10 (a): Incidence of Cotton foliar diseases as influenced by Technology for Organic Cotton Production (G. arboreum) 2015-16

Rust AB PDI Yield Sl. PDI Treatments (kg/ha) No 120 120

DAS** DAS** Cotton + 2 row of Lucerne & lopped at 25 days interval 32.20 33.00 T1 606 and incorporated in to the soil. (34.59) (35.04) Cotton + 2 rows of Stylosanthus scabra & lopped at 25 33.40 36.11 T2 1256 days interval and incorporated in to the soil. (35.27) (37.11) Cotton + Booadcasting of sunhemp at 45 days interval & 33.70 35.10 T3 433 incorporated in to the soil before flowering. (35.50) (36.34) Cotton + Booadcasting of dhaincha at 45 days interval & 33.60 35.10 T4 370 incorporated in to the soil before flowering. (35.44) (36.33) Cotton + Booadcasting of fodder cowpea at 45 days 35.10 34.90 T5 587 interval & incorporated in to the soil before flowering. (36.35) (36.19) Recommended dose of nutrients (60:30:30 NPK kg/ha) 33.50 33.90 T6 1225 through organics on P equivalent basis. (35.35) (35.58) 35.90 36.15 T7 Absolute control (No organics & No inorganics). 1054 (36.80) (37.15) Control (RDF through inorganic fertilizers i.e. 60:30:30 27.60 28.60 T8 1858 NPK kg/ha) (31.72) (32.33) Recommended organic protocol developed by UAS, 33.00 34.70 T9 1182 Dharwad. (35.07) (36.11) 34.50 35.10 T10 Farmers practice for organic cotton production. 1222 (35.95) (36.33) SEm± 0.87 0.90 71 CD at 5% 2.58 2.69 210

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 26 Table 11: Observations on the occurrence of various diseases in farmer’s field and research farms (Guntur) Hybrid Per cent disease Intensity (PDI) Month Location (Village/ Mandal) (BG II) Alternaria leaf spot Bacterial blight Grey mildew Rust Sept. Tadikonda Puja 0 0 0 0 Ponnekallu Bhakthi 0 0 0 0 Ravela Jadoo 0 0 0 0 75 Talluru Bhakthi 0 0 0 0 Lemalle ATM 0 0 0 0 China Agiripalli Jadoo 0 0 0 0 Sadananda 0 0 0 0 Bhakthi 0 0 0 0 Krishnapuram Bhakthi Traces 0 0 0 Raja Traces 0 0 0 Oct. Kesanupalli RCH 659 Traces 0 0 0 Dachepalli ATM 0 0 0 0 Jadoo 0 0 0 0 Raja Traces 0 0 0 Tallacheruvu Jadoo 24.36 0 0 0 ATM 4.5 0 0 0 Vempadu Jadoo 9 0 0 0 Sankarapuram Jadoo 2.5 0 0 0 Jarlapalem Jadoo 9.5 0 0 0 Unnava ATM Traces 0 0 0 Vankayalapadu Raja 13 0 0 0 Jadoo Traces 0 0 0 Timmapuram Jadoo 10.83 0 0 0 Tubadu ATM 8 0 5 0 Puja 7.5 0 0.5 0 Jadoo 4 0 0 0 Lingamguntlapalem Jadoo Traces 0 0 0 RCH 659 5 0 0 0 Yellamanda Bhakthi 13.75 0 0 0 Tadikonda Puja 18 0 24.5 0 Bayyavaram Jadoo 18.33 0 11 0 Acchampet Jadoo 10 0 15 0 Kesanupalli RCH 659 2.5 0 0 0 Gadevaripalli RCH 659 3 0 0 0 Nov. Kanchakacherla Jadoo 12 0 20 0 Bhakthi 6 0 16.5 0 Veldurthi RCH 659 19 0 22.5 Traces Puja 11.5 0 8.5 0 Sadananda 10.5 0 7 0 Uppalapadu Chandini 15 0 Traces 0 Mandadi Deltapine 9125 25 0 8.5 7.5 Kambhampadu Vardhini 999 2.5 0 0 0 Gurajala Puja 14 0 0 1 Jadoo 21.5 0 19 0 Preethi 333 3 0 0 0 Santhinagar Raja 14 0 20.5 0 Mathukumalli Bhakthi 24.5 0 24 0 Brahmanapalle Jadoo 7.5 8.5 12.5 0 Talarlapalle Jadoo 6.5 0 0 0 Bhakthi 10 0 0 0 Kubera 1.5 0 0 0 Kondramutla Jadoo 3 0 1 0 Dec Medikonduru RCH 659 31 0 25 0 Bhiminenivaripalem Puja 38 0 18 0 Jadoo 28 0 25 0 Raja 28 0 5 0 Kantepudi ATM 24 0 1 0 Jadoo 18 0 9 0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 27 Table 11 Contd. Hybrid Per cent disease Intensity (PDI) Month Location (Village/ Mandal) (BG II) Alternaria leaf spot Bacterial blight Grey mildew Rust Dhulipalla Puja 31 0 18 0 Nagireddypalem RCH 659 29 0 24 0 Superb 23 0 6 0 Bellamkonda ATM 23 0 1 0 Jadoo 25 0 25 0 Machayapalem ATM 22 0 8 0 Jadoo 41 0 17 0 Xylem 73C52 30 0 4 0 Kottaganeshunipadu RCH 659 23 0 15 0 ATM 23 0 3 0 Pandintipalem RCH 659 21 0 0 0 Julakallu Sidda 25 0 0 19 Karampudi Jadoo 27 0 0 0 Adigoppula RCH 659 19 0 0 0 Jadoo 29 0 15 0 Obulasunipalli Jadoo 35 0 15 0 Atmakuru Jadoo 18 0 0 Ankur 3224 27.5 0 7 0 Bhiravunipadu Puja 26 0 4 0 A Kondapuram Jadoo 8 0 0 0 Ajit 155 15 0 0 0 Govindarayunipet Ajit 155 21 0 0 0 Salakamcheruvu Jadoo 25 0 0 0 Nidanwada Mallika 10 0 0 0 Tarimela Ajit 155 5 0 0 0 Kalluru Ajit 155 7 0 0 20 Eddulapalli Sujatha 3 0 0 0 Jadoo 2 0 0 0 15 0 0 15 Gooty Jadoo 25 0 0 22 Kasepalli Raja 36 0 0 6 Pedvaduguru Jadoo 27 0 8 traces Hussinapuram Jadoo 7 0 0 0 Pedapadu Sujatha 29 0 19 21 Penchikalapadu Bhakthi 5 0 0 0 Karivemula Jadoo 43 0 6 0 Kunkanur Jadoo 35 0 0 0 Valigonda ATM 29 0 0 0 Sankarabanda Jadoo 6 0 0 0 Virupapuram Jadoo 5 18 0 0 Kallubavi Jadoo 7 0 0 0 Adoni Jadoo 38 0 0 0 Pandavgal Bhakthi 25 0 0 0 Basaldoddi Jadoo 21 0 1.5 0 Kambaladinne Jadoo 44 0 0 0 Kummarapalli Jadoo 54 0 3 0 Vengalayapalli Jadoo 5.5 0 4.5 0 Rajupalem Jadoo 58 0 3 0 Rangasaipalli Jadoo 36 0 0 0 Dharmapuram Ujjawal 4 0 5 3 Sujatha 0 0 10 21 Chinnakommerla JK 8633 2 0 18 30 Gandiganamala Jadoo 1 0 3 13 Ajit 155 6 0 13 21 Surepalli Jadoo 23 0 34 24 Nagarajupalli Jadoo 11 0 10 17 Kollapudi First class 20 0 30 10 Chinnagarnepudi Puja 25 0 5 traces Timmarajupalem Bhakthi 24 0 2 traces Kanchikacherla Raja 19 0 27 2

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 28

Table 11 (a) Abstract:

Mean Percent Disease Intensity (PDI) Month Hybrids (BG II) Alternaria Bacterial Grey Rust leaf spot blight mildew September Puja, Bhakthi, Jadoo, ATM, 0 to traces 0 0 0 2015 Sadananda, Raja, 0.5 to October RCH 659, ATM, Jadoo, Raja, Puja, 0 to 24.36 0 24.16 0 2015 Bhakthi (9.63) (12) Jadoo, Bhakthi, RCH 659, Puja, November Sadananda, Chandini, Deltapine 0 to 25 0 to 24.0 0 to 7.5 0 to 8.5 2015 9125, Vardhini 999, (11.5) (12.72) (4.25) Preethi 333, Raja, Kubera Puja, Bhakthi, Jadoo, ATM, Sadananda, Raja, RCH 659, Chandini, December Deltapine 9125, Vardhini 999, 0 to 58 0 to 34 0 to 30 0 to 18 2015 Preethi 333, Kubera, Superb, Xylem (21.29) (11.28) (16.27) 73C52, Sidda, Ankur 3224, Ajit 155, Force, Ujjawal, JK 8633, Bhakthi 0.0 - 0.0 – 54.0 0 to 0 - 55.0 RARS, Lam Varieties and Hybrids of cotton trials 54.0 (23.28) traces (21.34) (27.29)

Table 12: Observations on the occurrence of various diseases in farmer’s field and research farms (Coimbatore)

Root Grey Location TSV Alternaria leaf Bacterial leaf Rust Wilt rot mildew (%) blight (PDI) blight (PDI) (PDI) (%) (%) (PDI) Tirupur(RCH BGII) 12.0 3.2 6.0 0.0 0.0 0.0 0.0 Narasingapuram(RCH 5.0 3.8 2.0 0.0 0.0 0.0 0.0 BGII) Dharapuram(RCH BGII) 10.0 3.1 2.0 0.0 0.0 0.0 0.0 Ariyalur(RCH BGII) 12.0 2.2 5.0 0.0 0.0 0.0 0.0 Dharmapuri (RCH BGII) 10.0 2.0 5.0 0.0 0.0 0.0 0.0 Pottapathi(RCH BGII) 5.0 3.5 6.0 0.0 0.0 0.0 0.0 Erode(RCH BGII) 5.0 3.0 6.0 0.0 0.0 0.0 0.0 Anthiyur(RCH BGII) 5.0 2.0 6.0 0.0 0.0 0.0 0.0 Thondamuthur 10.0 3.8 3.0 0.0 0.0 0.0 4.0 Coimbatore 18.0 11.0 8.0 3.0 3.0 3.0 10.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 29

Path 1 (b) Disease progress in relation to weather factor

NORTH ZONE

Table 13: Disease progress in relation to weather factors at Faridkot (Punjab) (RS 921)

Temperature Relative humidity% FFLS Std RF BSS Wind CLCuD CLCuD % W/fly /3 T T Mea Rh Rh Mea BB week (MM) (hour) direction(º) PDI increase ALS MLS CLS leaves max min n max min n 25 37.3 27.7 32.5 69.3 40.9 55.1 28.1 4.9 106.1 131.8 0 0 0 0 0 0 2.37 26 36.6 24.8 30.7 75 48.7 61.9 75.5 6.2 196.1 176.8 2.67 2.67 0 0 0 0 4.13 27 35.7 27 31.3 76.3 58.7 67.5 97.5 2.7 102.9 147.9 5.37 2.7 0 0 0 0 8.83 28 33.2 26 29.6 87.1 66.3 76.7 18.9 4.4 147.9 125.4 9.5 4.13 0 0 0 0 12.5 29 34.7 27.1 30.9 79.9 62.4 71.1 19.3 4.6 160.7 112.5 15.5 6 0 0 0 0 17.33 30 34.3 26.8 30.5 81.9 70.3 76.1 26.3 5.7 112.5 135 35.2 19.7 0.1 0.1 0 0 26.17 31 32.6 26.4 29.5 85.9 74.7 80.3 16.7 3.6 125.4 125.4 42.1 6.9 0.1 0.2 0 0 38.17 32 34.6 27.8 31.2 88.6 74 81.3 16 2.6 147.9 154.3 47.43 5.33 0.3 0.4 0.1 0.3 47.63 33 34.8 27.3 31 84 67.4 75.7 34.3 6.4 163.9 225 56.37 8.94 0.5 1.1 0.1 0.3 53.5 34 34.3 26.5 30.4 81 63.1 72.1 51.2 7.9 192.9 186.4 70.87 14.5 1.6 1.2 0.7 0.5 57.5 35 35.7 26.7 31.2 76.6 52.9 64.7 0 9.1 196.1 270 79.97 9.1 1.7 1.4 0.8 0.6 27.87 36 35.4 24 29.7 75 47.3 61.1 0 9.5 228.2 282.9 81.8 1.83 1.7 1.4 0.9 0.7 24.83 37 36.8 25.1 30.9 75.1 44.9 60 0 9.4 173.6 196.1 83 1.2 1.9 1.6 1.2 1.1 19.63 38 31.5 22.8 27.2 85.1 68.9 77 41.4 5.1 80.4 212.1 84.67 1.67 2.1 2.1 1.7 1.2 12.17 39 33 21.6 27.3 81.4 47.9 64.6 0 9 231.4 205.7 85.17 0.5 2.2 2.1 1.8 1.6 3.57 40 35.1 20.4 27.7 81 41 61 0 9 189.6 225 85.8 0.63 2.4 2.2 2.2 1.9 2 41 35.4 21.7 28.5 79 43.3 61.1 0 7.4 118.9 183.2 86.8 1 2.4 2.3 2.2 2.1 1.57 42 32.1 18.9 25.5 83.6 46.6 65.1 0 4.9 205.7 279.6 87.23 0.43 2.4 2.4 2.3 2.2 0.3 43 30 15.6 22.8 84.4 35.3 59.9 1.9 6.1 225 215.4 87.23 0 2.5 2.5 2.4 2.3 0 44 28.7 13.7 21.2 83.7 41.1 62.4 0 4.3 199.3 266.8 87.23 0 2.5 2.6 2.4 2.4 0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 30

Table 14: Disease progress in relation to weather factors at Sriganganagar

Std. Disease Meteorological data Date of week incidence Rain fall Temperature (oC) RH (%) Observation No. (%) (mm) Max Min 8.30 am 17.30 pm 29.06.15 26 0.47 8.4 40.57 26.3 69.4 41.7 06.07.15 27 9.8 65.2 37.04 26.47 78.2 64.8 13.07.15 28 21.46 27.8 35.87 25.04 86.4 63.1 20.07.15 29 50.93 8.4 38.44 27.21 82.1 63.1 27.07.15 30 66.17 4.1 35.80 25.16 83.2 68.1 03.08.15 31 95.26 96.6 32.39 24.51 93.4 82.8 09.08.15 32 96.73 0 37.86 27.29 77.8 57.0 16.08.15 33 97.58 8.6 37.10 26.26 83.1 62.7 23.08.15 34 98.91 5.9 37.03 25.90 74 54.0 29.08.15 35 100 0 38.60 26.00 72.2 47.1

Table 14(a): Meteorological data at Sriganganagar

Rain fall Temperature (oC) Relative humidity (%) Std. week Week No. (mm) Max Min 8.30 am 17.30 pm 21 May-27 May 21 0.0 44.64 24.63 42.2 16.4 28 May-03 June 22 0.2 40.40 24.50 55.2 36.8 04 June-10 June 23 0.0 41.41 24.67 55.5 28.2 11 June-17 June 24 31.6 39.14 25.87 69.8 43.0 18 June-24 June 25 38.0 40.34 27.54 66.7 44.2 25 June-01 July 26 8.4 40.57 26.3 69.4 41.7 02 July-08 July 27 65.2 37.04 26.47 78.2 64.8 09 July-15 July 28 27.8 35.87 25.04 86.4 63.1 16 July-22 July 29 8.4 38.44 27.21 82.1 63.1 23 July-29 July 30 4.1 35.80 25.16 83.2 68.1 30 July-05 Aug. 31 96.6 32.39 24.51 93.4 82.8 06 Aug.- 12 Aug. 32 0.0 37.86 27.29 77.8 57.0 13 Aug.-19 Aug. 33 8.6 37.10 26.26 83.1 62.7 20 Aug.-26 Aug. 34 5.9 37.03 25.90 74.0 54.0 27 Aug.-02 Sept. 35 0.0 38.60 26.00 72.2 47.1 03 Sept.-09 Sept. 36 0.0 39.16 23.97 61.7 34.2 10 Sept.-16 Sept. 37 0.0 41.19 24.01 70.1 32.1

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 31

Table 15: Disease progress in relation to weather factors at Hisar

Temp. 0C RH % CLCuD WS CLCuD Std. Met. Week Bright sun hours/day Rain fall (mm) WF/3 leaves CLCuD (%) Difference BLB Max. Min. M E Km/hr index (%) 22 40.3 24.0 59.7 34.0 7.8 8.9 0.0 15.8 0.0 0.0 0.0 0.0 23 39.2 24.3 63.0 32.7 6.2 7.9 0.0 23.4 6.3 6.3 1.0 0.0 24 37.7 24.9 76.8 59.7 7.3 5.2 40.4 28.2 9.1 2.8 1.5 0.0 25 39.2 25.7 78.4 56.1 7.6 9.9 51.6 59.5 15.3 6.2 3.5 0.0 26 35.7 25.9 83.1 51.9 6.8 7.4 65.5 70.4 27.3 12.0 6.5 0.0 27 36.6 25.8 80.1 55.0 8.4 5.6 51.8 85.9 100 72.7 32.8 0.0 28 31.3 25.4 91.7 75.3 6.0 3.3 24.7 91.9 100 0.0 37.3 0.0 29 36.3 26.8 84.9 59.0 7.0 7.4 77.3 93.1 100 0.0 37.5 0.0 30 34.5 26.4 85.3 65.0 10.2 8.6 0.0 104.2 100 0.0 39.5 0.0 31 32.2 25.1 93.3 72.0 8.8 3.7 9.7 127.2 100 0.0 55.9 0.0 32 35.1 26.9 89.0 66.0 5.6 3.4 26.2 103.4 100 0.0 56.1 0.0 33 33.5 26.3 94.3 68.4 4.6 4.5 19.8 117.8 100 0.0 63.6 0.0 34 35.3 25.5 80.2 54.0 9.0 8.8 0.8 105.4 100 0.0 64.6 0.0 35 37.2 25.4 78.9 50.4 6.2 9.5 8.0 187.1 100 0.0 70.6 0.0 36 36.8 22.6 73.6 34.7 5.4 9.9 0.0 272.0 100 0.0 72.8 0.0 37 37.9 23.3 73.7 35.6 4.4 9.8 0.0 191.6 100 0.0 81.9 0.0 38 33.9 23.4 87.1 64.0 7.3 4.7 9.4 91.7 100 0.0 83.8 0.0 39 34.1 20.4 79.3 43.9 5.0 9.0 10.4 105.5 100 0.0 84.7 0.0 40 36.6 19.3 82.0 30.4 3.4 9.7 0.0 60.3 100 0.0 85.0 0.0 41 36.7 20.1 67.6 28.4 4.6 9.3 0.0 55.6 100 0.0 85.0 0.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 32

Table 16: Disease progress in relation to weather factors at Sirsa

MAX MIN RH RH BRI RAIN SMW % CLCuD incidence Whitefly/3 leaves Temp Temp (%) (%) SUN FALL °C °C M E HRS (Mm) 24 0.18 1.75 38.2 26.5 67.9 44.6 7.2 8.0 25 0.18 3.5 39.3 27.8 66.9 39.1 7.3 8.2 26 6.22 6.25 38.9 27.0 78.9 45.7 6.5 6.6 27 12.25 14.5 36.2 26.9 76.4 56.0 4.3 48.0 28 31.99 27 32.6 26.2 86.7 66.0 4.0 23.0 29 38.76 25.5 36.4 27.7 71.3 56.1 7.2 0.0 30 50.82 23.75 33.8 26.0 82.3 65.4 3.0 16.0 31 67.46 27.75 31.8 25.5 92.0 76.0 0.2 13.4 32 84.64 25.75 34.8 27.7 86.1 65.4 4.2 14.6 33 95.06 37 34.1 26.7 85.9 65.6 3.7 20.0 34 100.00 70 35.1 26.9 76.4 57.1 5.9 0.0

CENTRAL ZONE

Table 17: Disease progress in relation to weather factors at Rahuri

Met. Temp. Humidity Sun Rainy PDI RF(mm) Week Max Min Mor. Eve. shine Hrs. Days ALB 22 39.34 22.74 55.9 25.6 7.7 3.0 1 0 23 36.00 22.84 67.7 52.0 7.8 36.4 4 0 24 32.33 23.17 72.3 64.6 4.0 25.8 2 0 25 31.57 23.64 71.1 57.0 2.8 11.6 2 0 26 33.86 23.30 66.1 45.3 7.8 0 0 0 27 34.70 24.14 66.0 42.9 9.1 0 0 0 28 34.49 23.56 67.9 41.3 7.7 0 0 0 29 32.54 23.71 73.9 57.6 2.9 17.4 1 2.9 30 30.80 23.50 71.1 60.0 2.1 8.4 1 3.4 31 30.83 22.51 77.3 59.0 3.3 7.4 1 4.2 32 30.43 22.20 75.7 59.9 2.3 4.0 1 4.8 33 28.37 22.64 71.1 49.6 3.9 0 0 4.6 34 33.06 21.89 70.6 49.1 5.9 1.0 0 3.8 35 33.10 21.53 70.9 47.0 6.6 3.0 0 4.7 36 33.34 23.06 81.1 55.1 6.0 20.6 2 4.0 37 31.34 22.01 78.9 58.1 4.6 48.4 3 12.6 38 30.23 22.61 79.4 58.6 4.3 54.6 3 20.7 39 33.54 19.76 67.7 40.6 8.7 0 0 19.4 40 32.77 21.04 76.4 50.3 7.1 20.6 3 16.6 41 34.26 20.90 71.3 39.4 7.4 0 0 9.3 42 35.03 19.10 55.7 30.4 9.5 0 0 7.2

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 33

Table 17(a): Meteorological data at M.P.K.V.,Rahuri from Jan. 2015 to Dec. 2015

Met. week Date Temp Humidity Rain Fall (mm) Rainy day Eva. mm Max Min I II 1 1-7 25.0 12.4 74.3 55.3 1.0 0 4 2 8-14 26.9 6.8 49.4 26.1 0 0 5 3 15-21 28.6 10.5 54.6 33.0 0 0 5 4 22-28 29.4 14.1 62.6 37.6 0 0 5 5 29-4 30.1 12.9 51.7 35.3 0 0 5 6 5-11 31.3 12.3 50.9 23.9 0 0 6 7 12-18 33.0 13.0 53.0 22.7 0 0 6 8 19-25 33.2 13.6 54.3 23.7 0 0 7 9 26-4 30.1 13.0 57.6 37.3 42.4 2 6 10 5-11 31.9 14.8 52.4 37.6 22.8 1 6 11 12-18 31.0 16.8 67.1 39.1 11.0 2 6 12 19-25 35.3 19.0 45.3 23.3 0 0 7 13 29-01 36.3 19.4 53.7 20.6 0 0 8 14 02-08 35.8 16.1 51.0 20.0 0 0 8 15 09-15 33.6 19.5 65.4 36.9 7.2 1 7 16 16-22 37.2 21.6 47.7 21.9 0 0 7 17 23-29 39.1 20.8 40.3 13.6 0 0 8 18 30-06 40.4 22.0 36.0 16.3 0 0 9 19 07-13 39.7 24.4 41.0 19.1 0 0 9 20 14-20 38.6 23.5 48.4 27.7 8.0 1 9 21 21-27 40.0 23.6 49.4 22.6 0 0 12 22 28-03 39.3 22.7 55.9 25.6 3.0 1 9 23 04-10 36.0 22.8 67.7 52.0 36.4 4 8 24 11-17 32.3 23.2 72.3 64.6 25.8 2 5 25 18-24 31.6 23.6 71.1 57.0 11.6 2 4 26 25-01 33.9 23.3 66.1 45.3 0 0 7 27 02-07 34.7 24.1 66.0 42.9 0 0 8 28 08-14 34.5 23.6 67.9 41.3 0 0 7 29 15-21 32.5 23.7 73.9 57.6 17.4 1 6 30 22-28 30.8 23.5 71.1 60.0 8.4 1 5

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 34

Table 17(a) Contd.

Met. week Date Temp Humidity Eva. Rain Fall (mm) Rainy day Max Min I II mm 31 29-04 30.8 22.5 77.3 59.0 7.4 1 6 32 05-11 30.4 22.2 75.7 59.9 4.0 1 4 33 12-18 28.4 22.6 71.1 49.6 0 0 5 34 19-25 33.1 21.9 70.6 49.1 1.0 0 6 35 26-01 33.1 21.5 70.9 47.0 3.0 0 6 36 02-08 33.3 23.1 81.1 55.1 20.6 2 6 37 09-15 31.3 22.0 78.9 58.1 48.4 3 5 38 16-22 30.2 22.6 79.4 60.0 54.6 3 4 39 23-29 33.5 19.8 67.7 40.6 0 0 6 40 30-06 32.8 21.0 76.4 50.3 20.6 3 6 41 07-13 34.3 20.9 71.3 39.4 0 0 6 42 14-20 35.0 19.1 55.7 30.4 0 0 7 43 21-27 32.2 22.1 57.4 37.7 0 0 5 44 28-03 32.2 17.4 57.4 37.7 0 0 5 45 04-10 32.9 17.4 58.3 37.6 0 0 6 46 11-17 32.4 14.6 50.4 30.4 0 0 6 47 18-24 30.1 17.9 70.7 59.0 26.0 2 4 48 25-01 31.7 17.8 71.6 44.3 0 0 6 49 02-08 32.6 13.9 58.7 32.6 0 0 6 50 09-15 32.3 16.0 49.4 32.9 0 0 6 51 16-22 31.1 13.7 63.9 35.6 0 0 5 52 23-31 28.8 8.2 43.1 21.6 0 0 5 33.0 18.8 61.0 39.0 380.8 33 6.18

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 35

Table 18: Disease progress in relation to weather factors at Akola

R. Humidity Sunshine Per cent disease intensity Met. Rainy Rainfall Temperature 0C Period % hours LRA 5166 Bunny Bt AKA-8 Week days in mm Max. Min. Morn. Even BSH BLB MLS GM BLB MLS GM GM 36 3-9 Sept 2.0 22.4 32.9 22.5 87 57 7.4 4.1 1.6 - 3.6 Traces - - 37 10-16 2.0 61.6 34.3 22.7 88 54 6.6 5 2.08 - 3.8 Traces - - 38 17-23 1.0 78.9 31.3 22.2 87 63 6.1 4.11 - - 3.9 Traces - - 39 24-30 0.0 1.4 33.1 21.3 86 46 8.5 7.3 - - 4.8 Traces - - 40 1-7 Oct 0.0 0.0 35.6 20.8 84 39 8.1 7.9 - - 5 Traces - - 41 8-14 0.0 0.0 37.0 19.3 71 24 7.6 8.6 - - 5.5 Traces - - 42 15-21 0.0 0.5 36.5 19.1 71 37 8.3 6.35 - - 5.1 Traces - - 43 22-28 0.0 0.0 35.6 19.5 72 34 7.4 4.65 - - 4.6 Traces - - 44 29-4 Nov 0.0 0.0 32.9 18.1 77 36 7.5 4.3 - - 3.9 Traces 45 5-11 0.0 0.0 33.8 17.0 72 28 7.1 4.5 - - 3.1 - 46 12-18 0.0 0.0 33.5 15.2 73 26 7.8 1.6 - - 1.6 - 47 19-25 0.0 0.0 32.4 16.6 67 34 6.0 0.83 - - 1.6 - (Figures in parenthesis are arc sin value)

Table 18 (a) Akola: Correlation values between weather parameters and bacterial blight of cotton at Akola (MS.) (2015-2016)

Temperature 0C R. Humidity Sr. No. Particulars Rainfall Sunshine hours/BSH Max. Mini Morn. Even. LRA-5166 1 -0.108 0.642 0.642 0.258 -0.0225 0.591 % disease intensity Bunny Bt 2 -0.017 0.572 0.572 0.255 0.102 0.513 % disease intensity (‘r’) values : * Significant at 5% 0.456, ** Significant at 1% 0.575

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 36

Table 19: Observations on the occurrence of various diseases in trap nursery (Khandwa)

BLB MLB GM Month MSW Max. grade PDI Max. grade PDI Max. grade PDI June 22 23 24 25 1 Traces 1 Traces July 26 1 Traces 1 Traces - - 27 1 Traces 1 2.33 - - 28 1 2.73 1 4.77 - - 29 1 4.33 1 6.33 - - 30 1 6.12 1 6.63 - - August 31 1 8.91 2 10.67 - - 32 2 9.74 2 12.17 - - 33 2 9.74 2 12.83 - - 34 2 10.16 2 12.50 - - September 35 3 18.77 3 15.33 - - 36 3 18.21 3 15.17 - - 37 3 15.70 2 12.83 - - 38 3 15.15 2 10.67 - - 39 2 14.73 2 10.00 1 5.33 October 40 1 7.87 1 9.33 1 5.67 41 1 4.73 1 6.50 2 7.33 42 1 4.45 1 5.33 2 8.67 43 1 3.00 1 3.67 2 12.67 November 44 1 2.33 1 3.33 2 9.83 45 1 2.33 1 3.33 2 9.83 46 1 2.33 1 3.33 2 9.67 47 1 8.33 December 48 1 6.67 49

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 37

Table 20: Disease progress in relation to weather factors at Surat

Weather parameter Temp Humidity Sun STD Met Week BLB PDI Rainfall Rainy days shine Max Min Morning Evening hr 25 0.0 32.1 28.1 93.0 84.0 209.8 3 2.6 26 0.0 31.5 29.2 82.0 76.0 0 0 5.3 27 0.0 34.8 30.3 80.0 74.0 0 0 5.5 28 2.0 32.9 29.8 78.0 73.0 2.0 1 5.3 29 2.0 33.1 29.2 86.0 81.0 40.2 2 3.2 30 2.5 30.1 27.7 90.0 86.0 93.0 6 1.4 31 2.5 30.6 29.1 88.0 84.0 0 0 2.0 32 3.0 31.8 28.3 89.0 75.0 2.4 1 2.8 33 3.0 31.9 28.1 86.0 75.0 6.4 2 2.6 34 3.5 32.0 28.5 87.0 73.0 0 0 4.2 35 4.0 33.1 28.5 89.0 71.0 18.2 1 5.3 36 6.0 32.5 26.9 86.0 69.0 0 0 7.4 37 8.5 32.1 28.4 89.0 82.0 24.4 2 6.4 38 14.5 31.1 27.3 95.0 88.0 150.0 3 3.4 39 23.5 32.6 26.7 91.0 68.0 0 0 7.0 40 18.0 36.1 27.5 80.0 68.0 0 0 7.1 41 15.5 36.6 27.1 85.0 64.0 0 0 7.0 42 10.5 37.3 27.1 76.0 63.0 0 0 6.8 43 8.5 36.4 25.7 78.0 57.0 0 0 6.0 44 7.0 34.9 24.0 80.0 53.0 0 0 6.0 45 5.5 32.4 23.3 74.0 70.0 0 0 4.9 46 4.0 34.9 24.0 75.0 70.0 0 0 5.4 47 3.5 34.7 23.9 79.0 66.0 0 0 6.6 48 3.0 34.9 23.4 75.0 72.0 0 0 6.5 49 2.0 33.6 18.7 70.0 60.0 0 0 6.7 50 0.0 33.3 19.3 71.0 62.0 0 0 7.3 51 0.0 31.1 16.7 68.0 61.0 0 0 7.8 52 0.0 33.2 16.2 70.0 62.0 0 0 7.9 Correlation matrix 0.3366 0.2094 0.3264 -0.1019 -0.0219 -0.0873 0.2493

*significant at 5 % and ** 1% level of significance at n-2 degree of freedom

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 38

Table 21: Disease progress in relation to weather factors at Junagadh

Period Percent disease Weather Parameters (2015-16) incidence(PDI) Mon. Std. Temperature Relative Humidity 0 Rainfall Rainy Date Met. ALS BLB GM C (%) mm Days Week Max Min Morn. Even. Jun 04-10 23 0.00 0.00 0.00 38.9 26.6 79 39 15.6 2 Jun 11-17 24 0.00 0.00 0.00 38 26.6 85 59 11.8 1 Jun 18-24 25 0.00 0.00 0.00 33.6 25.8 87 87 178 5 Jun 25-01 26 0.00 0.00 0.00 33.4 26.3 84 64 5 1 July 02-08 27 0.00 0.00 0.00 34.8 26.5 82 61 0 0 July 09-15 28 0.00 0.00 0.00 34.9 26.8 85 74 0 0 July 16-22 29 0.00 0.00 0.00 33.4 26.6 87 74 6.6 0 July 23-29 30 0.00 0.00 0.00 29.4 24.9 95 92 272.8 7 July 30-05 31 0.00 0.00 0.00 30.9 25.4 90 80 4 0 Aug 6 -12 32 0.00 1.00 0.00 32.1 25.1 88 71 0.0 0 Aug 13 -19 33 0.00 1.00 0.00 33 24.9 90 64 11.6 2 Aug 20 -26 34 0.00 1.00 0.00 32.1 25.4 87 66 3.8 0 Aug 27- 2 35 0.00 1.00 0.00 32.9 24.8 83 57 0.0 0 Sept 3 - 9 36 0.00 1.00 0.00 34.1 24 78 46 0.0 0 Sept 10 -16 37 0.00 1.00 0.00 33.9 24.7 82 57 94.5 1 Sept 17 -23 38 0.00 1.20 0.00 31.5 24.7 92 81 119.8 4 Sept 24 -30 39 1.00 1.30 0.00 33.2 22.7 81 66 0.0 0 Oct 1 - 7 40 1.00 1.30 0.00 37.5 24.3 76 45 0.0 0 Oct 8 -14 41 1.20 1.30 0.00 37.3 24.4 80 58 10.4 1 Oct 15 -21 42 1.50 1.50 0.00 38.2 23.7 71 49 0.0 0 Oct 22 -28 43 1.90 1.60 0.00 37.4 21.2 64 34 0.0 0 Oct 29 - 4 44 3.20 2.40 0.00 35.2 18.6 67 39 0.0 0 Nov 5 -11 45 13.30 5.00 0.00 35.5 17.1 70 30 0.0 0 Nov 12 -18 46 17.20 7.30 0.00 35.8 18.4 66 35 0.0 0 Nov 19 -25 47 12.00 9.00 0.00 35.4 19.4 70 47 0.0 0 Nov 26 -2 48 12.40 8.20 0.00 32.3 16.7 68 33 0.0 0 Dec 3 - 9 49 14.60 9.40 0.00 33.7 14 66 29 0.0 0 Dec 10 -16 50 13.70 6.70 0.00 29.8 12 64 29 0.0 0 Dec 17 -23 51 9.60 3.60 0.00 29.1 11.2 55 22 0.0 0 Dec 24 -31 52 11.30 4.40 0.00 32.1 12.4 64.4 30.8 0.0 0 Jan 1-7 1 8.00 1.00 0.00 33.5 13.8 74 30 0.0 0 Jan 8-14 2 5.00 1.00 0.00 30.2 12.3 78 33 00 0

ALS-Alternaria leaf spot, BLB-Bacterial leaf blight & GM-Gray mild

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 39

Table 21 (a): Correlation values between weather parameters and alternaria leaf spot and bacterial leaf blight of cotton at Junagadh (2015-16) [Correlation coefficient(r)]

Temperature 0C Relative Humidity (%) Sr No Particulars Rainfall mm Rainy Days Maxi Mini Morning Evening 1 Alternaria leaf spot -0.139 -0.826 -0.761 -0.718 -0.272 -0.324 2 Bacterial leaf blight -0.074 -0.669 -0.690 -0.606 -0.258 -0.308 'r') values * Significant at 5 % (P at 0.05) : 0.433 ** Significant at 1 % (P at 0.01): 0.549

SOUTH ZONE

Table 22: Disease progress in relation to weather factors at Dharwad

Temperature o Rainfall Rainy RH Morning RH Evening Date C (mm) Days (%) (%) Max Min 30th Jul -05th Aug. 26.3 21.6 93 80 7.4 2 6th Aug – 12th Aug 28.2 21.1 92 69 8.0 - 13th Aug – 19th Aug 29.2 21.0 91 64 0.6 - 20th Aug – 26th Aug 29.9 21.3 90 61 13.0 2 27th Aug – 2nd Sep 28.5 21.2 91 67 10.6 2 03rd Sep – 9th Sep 27.6 20.5 93 72 65.6 3 10th Sep - 16th Sep 28.3 20.5 92 65 5.8 1 17th Sep – 23rd Sep 27.6 20.7 93 72 - - 24th Sep – 30th Sep 30.0 21.1 s88 60 - - 1st Oct – 7th Oct 28.9 20.3 90 65 127.2 4 8th Oct – 14th Oct 29.4 20.0 92 61 1.6 - 15th Oct – 21st Oct 29.3 20.4 97 73 4.6 1 22nd Oct – 28th Oct 28.5 21.1 95 72 - - 29 Oct - 04th Nov 30.2 20.5 69 60 1.0 - 05th Nov – 11th Nov 32.5 20.9 90 53 2.2 - 12th Nov -18th Nov 29.0 20.2 94 69 - -

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 40

Table 22(a): Dharwad: Observations on the appearance and progress of foliar diseases of cotton in disease trap nursery

Sl. Std Met V1 Abhadita V2 Jayadhar V3 Bunny Bt V4 RCH 2Bt No Weeks AB BB GM Rust AB GM Rust AB BB GM Rust AB BB GM Rust 06.70 0.00 0.00 0.00 08.60 0.00 0.00 13.90 0.00 0.00 0.00 07.00 0.00 0.00 0.00 1 31 (15.04) (0.00) (0.00) (0.00) (17.01) (0.00) (0.00) (21.88) (0.00) (0.00) (0.00) (15.28) (0.00) (0.00) (0.00) 08.80 0.00 0.00 0.00 08.70 0.00 0.00 11.20 0.00 0.00 0.00 09.90 0.00 0.00 0.00 2 32 (17.21) (0.00) (0.00) (0.00) (17.18) (0.00) (0.00) (19.54) (0.00) (0.00) (0.00) (18.29) (0.00) (0.00) (0.00) 09.90 0.00 0.00 0.00 08.50 0.00 0.00 09.40 0.00 0.00 0.00 09.10 0.00 0.00 0.00 3 33 (18.34) (0.00) (0.00) (0.00) (16.95) (0.00) (0.00) (17.89) (0.00) (0.00) (0.00) (17.51) (0.00) (0.00) (0.00) 10.60 0.00 0.00 0.00 10.60 10.20 0.00 11.10 0.00 10.20 0.00 09.20 0.00 0.00 0.00 4 34 (19.01) (0.00) (0.00) (0.00) (19.01) (18.63) (0.00) (19.43) (0.00) (18.63) (0.00) (17.62) (0.00) (0.00) (0.00) 11.40 0.00 0.00 0.00 13.90 14.80 0.00 12.20 0.00 0.00 0.00 11.30 0.00 0.00 0.00 5 35 (19.71) (0.00) (0.00) (0.00) (21.92) (22.69) (0.00) (20.45) (0.00) (0.00) (0.00) (19.65) (0.00) (0.00) (0.00) 16.30 0.00 0.00 0.00 11.00 12.60 0.00 14.50 0.00 0.00 0.00 12.40 0.00 0.00 0.00 6 36 (23.81) (0.00) (0.00) (0.00) (19.36) (20.78) (0.00) (22.41) (0.00) (0.00) (0.00) (20.62) (0.00) (0.00) (0.00) 14.50 0.00 0.00 0.00 10.60 11.70 0.00 16.60 0.00 0.00 0.00 18.40 0.00 0.00 0.00 7 37 (22.38) (0.00) (0.00) (0.00) (19.00) (20.01) (0.00) (24.06) (0.00) (0.00) (0.00) (25.37) (0.00) (0.00) (0.00) 15.60 05.20 04.70 0.00 14.10 17.30 0.00 19.10 0.00 0.00 0.00 18.00 0.00 0.00 0.00 8 38 (23.28) (13.12) (12.51) (0.00) (22.00) (24.58) (0.00) (25.93) (0.00) (0.00) (0.00) (25.15) (0.00) (0.00) (0.00) 20.00 04.90 07.70 0.00 20.60 28.20 0.00 17.90 04.90 0.00 0.00 17.30 03.80 0.00 0.00 9 39 (26.56) (12.76) (16.11) (0.00) (26.97) (32.09) (0.00) (24.99) (11.45) (0.00) (0.00) (24.58) (11.20) (0.00) (0.00) 21.90 07.10 07.70 0.00 20.90 25.90 0.00 23.30 04.30 0.00 0.00 26.10 04.60 0.00 0.00 10 40 (27.88) (15.43) (16.39) (0.00) (27.20) (30.56) (0.00) (28.85) (11.93) (0.00) (0.00) (30.72) (12.34) (0.00) (0.00) 22.90 08.50 08.40 0.00 21.30 25.30 0.00 26.30 05.20 0.00 0.00 26.70 05.80 0.00 0.00 11 41 (28.62) (16.99) (16.87) (0.00) (27.47) (30.23) (0.00) (30.82) (13.14) (0.00) (0.00) (31.10) (13.89) (0.00) (0.00) 27.80 10.70 04.30 0.00 29.50 44.80 0.00 29.20 06.70 0.00 0.00 18.30 03.80 0.00 0.00 12 42 (31.85) (19.11) (10.41) (0.00) (32.88) (42.03) (0.00) (32.74) (15.03) (0.00) (0.00) (25.29) (11.23) (0.00) (0.00) 25.30 11.60 04.00 0.00 25.90 29.60 0.00 26.20 06.40 07.30 0.00 25.90 06.00 05.50 0.00 13 43 (30.22) (19.95) (10.06) (0.00) (30.56) (32.97) (0.00) (30.80) (14.63) (15.62) (0.00) (30.58) (14.22) (13.58) (0.00) 31.30 11.60 11.50 15.00 26.40 29.10 07.20 31.80 07.20 07.80 15.10 31.80 07.50 07.80 10.90 14 44 (34.05) (19.93) (19.78) (22.76) (30.91) (32.67) (15.59) (34.35) (15.56) (16.18) (22.87) (34.32) (15.91) (16.19) (19.25) 33.40 11.90 11.90 15.40 28.20 29.50 10.40 34.10 07.50 08.60 15.60 31.90 06.20 07.40 12.40 15 45 (35.32) (20.15) (20.15) (23.09) (32.07) (32.91) (18.79) (35.76) (15.88) (17.03) (23.24) (34.37) (14.38) (15.75) (20.65) 32.20 11.90 12.70 23.00 28.70 26.80 13.90 32.90 07.20 04.40 30.50 34.40 08.00 07.30 20.80 16 46 (34.55) (20.19) (20.88) (28.64) (32.40) (31.17) (21.88) (34.99) (15.53) (12.06) (33.49) (35.90 (16.45) (15.63) (27.15) * Figures in parentheses indicate angular transformed value

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 41

Table 22(b) Dharwad: Correlation values for foliar diseases of cotton with weather parameters Sl. no r’values V1 Abhadita V2 Jayadhar V3 Bunny Bt V4 RCH 2Bt weather parameters AB BB GM Rust AB GM Rust AB BB GM Rust AB BB GM Rust 1 Max. Temp 0.614 0.496 0.561 0.532 0.574 0.494 0.547 0.495 0.593 0.575 0.495 0.530 0.569 0.494 0.518 2 Min. Temp -0.619 -0.523 -0.553 -0.284 0.507 -0.570 -0.273 -0.585 -0.479 0.096 -0.300 -0.667 -0.502 -0.146 -0.289 3 RH Morning (%) -0.256 -0.186 -0.318 -0.429 0.473 -0.125 -0.378 -0.211 -0.232 -0.364 -0.380 -0.291 -0.278 -0.390 -0.388 4 RH Evening (%) -0.405 -0.272 -0.441 -0.387 0.401 -0.326 -0.397 -0.270 -0.366 -0.416 -0.350 -0.430 -0.388 -0.299 -0.371 5 Total Rainfall 0.258 0.187 0.275 0.649 0.527 -0.057 0.692 0.353 0.224 0.353 0.649 0.278 0.221 0.514 0.663 6 Rainy Days -0.128 -0.109 -0.114 0.139 0.517 -0.268 0.152 0.001 -0.023 -0.180 0.179 -0.144 -0.043 -0.064 0.164 Critical Values of the Pearson Product-Moment Correlation Coefficient (df:n-2) = 0.497.

Table 23: Disease progress in relation to weather factors at Guntur Percent Disease Intensity (PDI) Temperature Relative Rain Sun Wind Rainy Alternaria leaf spot Grey mildew Rust SW (0C) Humidity (%) Fall shine speed Evaporation days Jadoo RCH 2 Bunny Jadoo Bunny Jadoo RCH 2 Bunny (mm) hours km/h Max Min I II BG II BG II BG II BG II BG II BG II BG II BG II 36 33.33 24.89 88 68.14 93.8 4.86 5.83 4.86 7.43 10 6.5 5.5 37 32.71 24.21 83.71 77.57 46.8 5 3.07 3.4 1.14 14 12 11.5 38 32.79 25.93 97.71 70.43 66.7 3 3.14 6.14 2.71 12.5 14.5 13.5 39 33.77 25.88 90.71 64.43 34.4 2 6.71 4.33 4.14 12.5 13.5 11 40 33.9 24.79 97.71 70.14 5.1 1 3 2.43 2.71 11 12 13 41 37.28 25.5 91.43 52.29 9.7 1 6.57 3.43 8.29 13.5 14 15 42 37 24.93 93.14 61.14 4.9 1 7.14 3.71 6.57 15.5 14.5 15 43 35.91 23.64 90.43 56 2.8 1 8.43 3.43 9 15 15 16 44 35.51 23.57 92 58.86 0 0 6.29 4.71 9.71 16.5 15 16.5 3 45 33.5 23.21 90.43 57.43 7.2 1 5.71 5.43 8.86 16.5 15 15.5 5.5 46 31.57 20 96.29 65.43 60.8 2 3.57 4.43 6.71 15.5 15 17 12 47 30.78 20.07 98.29 69.43 5.0 1 1 4.86 3.14 22.5 23.5 24.5 24 48 30.93 18.57 98.29 76.29 0.1 0 4.71 4 6.71 27 21 23.5 35 10 49 31.86 18.29 99.43 82 0 0 5.71 2.71 5.57 25 25 22.5 45 18 10 10 7.5 50 32.36 17.5 96.57 63.43 0 0 5.14 4.14 5.14 24 23 20 52 25 11.5 11 12.5 51 32.71 18.29 98.86 64 0 0 6.14 3.86 5.43 22 22 18 52.5 34 16 13.5 15 52 32 16.88 97.5 58.38 0 0 3.38 3.88 3 16 14 10 50.5 25.5 19.5 20 18 1 31.57 16.43 98.86 55.57 0 0 4.86 3.14 5 11 7.5 7 51.5 23 23 27.5 20 2 31.57 15.64 99.43 50.71 0 0 7 3.14 3.71 51 20.5 32 37 28 3 30.79 17 98.86 57.29 0 0 6.57 3.86 3.14 49.5 20.5 39 42 37 4 30.71 18.21 96 55 0 0 3.14 4.34 3.14 48.5 20.5 41 45 35

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 42

Table 23(a): Regression Analysis of Alternaria leaf spot, Grey mildew and Rust diseases in relation to weather parameters during at Guntur

Disease Hybrid Regression Equation r2 (%) Y= -39.2768+1.307769 max T - 1.22203 min T*- 0.11079 RH I+ 0.57482 RH II**- 0.09548 RF- 87.65 0.17934 RD +0.033256 SSH+ 2.944065 WS* +0.361734 Evap. Jadoo BG II Y= -26.9967 +1.451602 max T - 1.26855 min T - 0.27721 RH I +0.591746 RH II** -0.06128 RF - 86.87 1.18856 RD +0.147242SSH +3.106494WS** Alternaria leaf Y= - 82.64 + 2.87 max T**– 2.1 min T** + 0.58 RH II** + 2.89 WS* 72.80 spot Y= - 68.8241+1.612544 max T - 0.87676 min T + 0.035931 RH I + 0.557869 RH II** - 0.0809 RF - 73.76 RCH 2 BG II 0.74297 RD + 0.048174 SSH + 3.179316 WS*- 0.06694 Evap. Y= -85.5128 + 2.805022 max T* -1.84802 min T**+ 0.566598 RH II **+ 2.78101 WS 54.11 Y= -21.5626 +0.462821 RH II** -0.11685 RF - 0.52154 RD-0.67247 SSH + 1.801627 WS Bunny BG II 71.65 +1.007183Evap Jadoo BG II Y = 237.1407 + 0.784002 max T - 8.09202 min T** - 0.88952 RH I + 0.227871 RH II -0.28859 RF* 93.16 Grey mildew Bunny BG II Y = -400.051 + 7.849786 max T** + 4.643328 min T* + 1.37558 RH I - 0.67475 RH II** 92.64 Jadoo BG II Y = 440.8675 -12.127 max T* - 0.53435 RH II * 91.33 Rust RCH 2 BG II Y = 528.4587 -14.6223 max T** - 0.64536 RH II** 95.53 Bunny BG II Y = 125.6862 - 9.69779 max T** + 4.378865 min T +1.842937 RH I - 0.87668RH II * 94.59 *significant at 5%; ** Significant at 1% level

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 43

Table 24: Disease progress in relation to weather factors at Coimbatore

Std. Foliar diseases Soil borne disease Week Alternaria Leaf spot (PDI) TSV incidence (%) Root rot (%) No. MCU13 RCHBGII TCH32 MCU13 RCHBGII TCH32 MCU13 RCHBGII TCH32 31 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 32 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 33 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 34 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 35 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 36 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 37 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 38 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 39 2.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 40 2.0 0.0 0.0 0.0 0.0 0.0 5.0 0.0 0.0 41 2.0 0.0 0.0 0.0 0.0 0.0 5.0 0.0 0.0 42 2.0 3.0 3.0 0.0 0.0 0.0 5.0 0.0 0.0 43 3.0 3.0 3.0 0.0 0.0 0.0 10.0 0.0 0.0 44 3.0 3.0 4.0 0.0 0.0 0.0 10.0 0.0 0.0 45 4.0 5.0 5.0 0.0 0.0 0.0 15.0 0.0 0.0 46 5.0 6.0 5.0 3.0 4.0 6.0 15.0 0.0 0.0 47 5.0 7.0 8.0 5.0 6.0 9.0 15.0 0.0 0.0 48 5.0 7.0 8.0 5.0 6.0 9.0 15.0 0.0 0.0 49 6.0 8.0 8.0 5.0 7.0 9.0 15.0 0.0 0.0 50 6.0 8.0 8.0 6.0 8.0 9.0 15.0 0.0 0.0 51 7.0 8.0 8.0 7.0 8.0 9.0 15.0 0.0 0.0 52 7.0 8.0 8.0 7.0 8.0 9.0 15.0 0.0 0.0 1 8.0 8.0 8.0 7.0 9.0 9.0 15.0 0.0 0.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 44 Path 1 (C): Studies on variability of Alternaria leaf spot.

Table 25: Studies on variability of Alternaria leaf spot at Junagarh Symptoms of Alternaria Leaf blight S. No. Genotypes Dark brown with Dark brown without Regular necrotic Irregular necrotic Conidia characters concentric rings concentric rings spots spots 1. 767 + - + - Light brown with multiseptate 2. 513 - + - + Light brown with multiseptate 3. 765 - - + - Dark brown with multiseptate 4. 5514 Dark brown with multiseptate 5. 5513 Dark brown with multiseptate 6. 763 + - - - Light brown with multiseptate 7. 769 - - - + Light brown with multiseptate 8. 717 + - - - Light brown with multiseptate 9. 5526 - - - + Dark brown with multiseptate 10. 5522 - + - + Dark brown with multiseptate 11. 533 + - + - Dark brown with multiseptate 12. 532 + - + - Dark brown with multiseptate 13. 501 - + - + Dark brown with multiseptate 14. 523 - - - + Dark brown with multiseptate 15. 505 + - + - Dark brown with multiseptate 16. 529 + - + - Dark brown with multiseptate 17. 509 - + - + Dark brown with multiseptate 18. 527 + - + - Dark brown with multiseptate 19. 536 - + - + Dark brown with multiseptate 20. 4538 - + - + Dark brown with multiseptate 21. 4515 + - + - Dark brown with multiseptate 22. 4511 - + - + Dark brown with multiseptate 23. 4509 - + - + Dark brown with multiseptate 24. 4507 - - + - Dark brown with multiseptate 25. 4532 + - - - Dark brown with multiseptate

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 45

Table 26: Studies on variability of Alternaria leaf spot at Dharwad

Description of Ellis M.B. regarding Resemblance Size of No. of No. of Overall Alterneria macrospora Zimm. towards Sr. Name of conidia(um) Beak horizontal vertical length No. of No. of Alterneria no isolate Length x length Overall septa septa (um) horizontal vertical Beak length macrospora breadth length(um) septa septa Zimm. Equal or twice Not Several 1 Dharwad 61.98x30.08 4 3 17.40 79.38 4-9 the length of 90-180 resembling to septa conidia A.macrosora Equal or twice Several resembling to 2 Khalaghatgi 72.73x12.05 4 2 26.03 98.76 4-9 the length of 90-180 septa A.macrosora conidia Equal or twice Not Several 3 Garag 42.43x17.69 3 1 23.45 65.88 4-9 the length of 90-180 resembling to septa conidia A.macrosora Equal or twice - Several resembling to 4 Murgod 73.89x15.69 5 1 28.64 102.53 4-9 the length of 90-180 septa A.macrosora conidia Equal or twice Hebballi Several resembling to 5 68.58x19.31 5 2 24.01 92.59 4-9 the length of 90-180 farm septa A.macrosora conidia Equal or twice Several resembling to 6 gadag 68.85x28.07 4 1 23.81 92.64 4-9 the length of 90-180 septa A.macrosora conidia

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 46

Table 27: Studies on variability of Alternaria leaf spot at Guntur

S. No. Date Place Hybrid / variety Presence of Alternaria spores 1 05.09.15 RARS, Lam Narasimha + 2 01.10.15 Dachepalli RCH 659 + 3 06.10.15 Tallacheruve Jadoo + 4 16.10.15 RARS, Lam Narasimha + 5 28.10.15 Tadikonda Jadoo + 6 05.11.15 Veldurthi RCH 659 + 7 08.11.15 Mathukumalle Bhakthi + 8 27.11.15 RARS, Lam 2a entries + 9 1.12.15 Medikonduru RCH 659 + 10 Kantepudi ATM + 11 Machayapalem Jadoo + 12 Pandintipalem RCH 659 + 13 07.12.15 Karivemula Jadoo + 14 Kambaladinne Jadoo + 15 08.12.15 Kummarapalli Jadoo + 16 Rajupalem Jadoo +

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 47

Table 28: Studies on variability of Alternaria leaf spot at Coimbatore Symptoms of Alternaria Leaf blight S. Genotypes Dark brown with Dark brown without Regular necrotic Conidia characters No. Irregular necrotic spots concentric rings concentric rings spots 1. 767 + - + - Light brown with multiseptate 2. 513 - + - + Light brown with multiseptate 3. 765 - - + - Dark brown with multiseptate 4. 5514 Dark brown with multiseptate 5. 5513 Dark brown with multiseptate 6. 763 + - - - Light brown with multiseptate 7. 769 - - - + Light brown with multiseptate 8. 717 + - - - Light brown with multiseptate 9. 5526 - - - + Dark brown with multiseptate 10. 5522 - + - + Dark brown with multiseptate 11. 533 + - + - Dark brown with multiseptate 12. 532 + - + - Dark brown with multiseptate 13. 501 - + - + Dark brown with multiseptate 14. 523 - - - + Dark brown with multiseptate 15. 505 + - + - Dark brown with multiseptate 16. 529 + - + - Dark brown with multiseptate 17. 509 - + - + Dark brown with multiseptate 18. 527 + - + - Dark brown with multiseptate 19. 536 - + - + Dark brown with multiseptate 20. 4538 - + - + Dark brown with multiseptate 21. 4515 + - + - Dark brown with multiseptate 22. 4511 - + - + Dark brown with multiseptate 23. 4509 - + - + Dark brown with multiseptate 24. 4507 - - + - Dark brown with multiseptate 25. 4532 + - - - Dark brown with multiseptate

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 48

Path 1 (d): Survey and Epidemiology of TSV

Table 29: Occurrence of TSV at Rahuri Locations Variety/hybrids % Incidence Bhakti 1.30 Ajit 199 2.50 Rahuri Ajit 155 1.95

MRC 7351 3.84 Jadoo(Kaveri) 4.53 Bt No. 228 2.0 Sangmner Ajit 199 1.90 Rashi 2 Bt 3.07 Jadoo(Kaveri) -- Dhule Trinetra 1.95 Mallika 1.00 MRC 7351 2.27 MRC 7351 2.20 NCS 245(Bhakti) -- Jalgaon Jadoo (Kaveri) 1.90 Ajit 199 2.43 Chamatkar --- MRC 7347 3.00 Ajit 199 -- Nandurbar MRC 7351 3.85 Mallika 2.77 Pancham 2.23

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 49

Table 30: Occurrence of TSV at Farmers field Guntur

Month Location (Village/ Mandal) Hybrid (BG II) TSV (%) September Tadikonda Jadoo 2 Ponnekallu Bhakthi 10 Ravela Jadoo 1 75 Talluru ATM 1 Lemalle Jadoo <1 Amaravathi Bhakthi traces China Agiripalli Bhakthi traces Krishnapuram Puja 0 October Dachepalli RCH 659 0 Kesanupalli Vardhini 999 0 ATM traces Raja 0 Tallacheruvu Jadoo traces Unnava ATM 0 Jadoo 0 Vankayalapadu Puja traces Tubadu ATM traces Jadoo traces Lingamguntlapalem Jadoo 0 ATM 0 Bayyavaram Jadoo 0 Gadevaripalli Jadoo traces November Kanchakacherla Raja 0 Veldhurthi RCH 659 0 Puja traces Chandini 0 Gurajala Puja 0 Preethi 333 0 Bondilipalem Bhakthi traces Jadoo 0 Kondrumutla Jadoo 3 December Medikonduru RCH 659 0 Bhiminenivaripalem Puja 0 Kantepudi ATM 0 Dhulipalla Bhakthi traces Nagireddypalem Superb traces Bellamkonda RCH 659 0 Papayapalem ATM traces Pandintipalem RCH 659 traces Adigoppula RCH 659 0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 50 Table 30: Contd.. Month Location (Village/ Mandal) Hybrid (BG II) TSV (%) Pothepalli Jadoo 0 Ankur 3224 0 A Kondapuram Jadoo 0 Ajit 155 0 Govindarayunipet Ajit 155 0 Salakamcheruvu Jadoo 0 Nidanwada Mallika 0 Tarimela Ajit 155 traces Kalluru Ajit 155 3 Eddulapalli Sujatha 0 Jadoo 3 Force 0 Gooty Jadoo 0 Kasepalli Raja 0 Pedvaduguru Jadoo traces Hussinapuram Jadoo 1 Pedapadu Sujatha 0 Penchikalapadu Bhakthi 0 Karivemula Jadoo 0 Kunkanur Jadoo 0 Valigonda ATM 0 Sankarabanda Jadoo 0 Erupapuram Jadoo 0 Kallubavi Jadoo 0 Adoni Jadoo 3 Pandavgal Bhakthi 0 Basaldoddi Jadoo traces Kambaladinne Jadoo 0 Emmiganur Jadoo 1 Kummarapalli Jadoo 3 Vengalayapalli Jadoo 0 Rajupalem Jadoo 0 Rangasaipalli Jadoo 0 Dharmapuram Ujjawal 0 Sujatha 1 Chinnakommerla JK 8633 0 Gandiganamala Jadoo 6 Ajit 155 0 Surepalli Jadoo 9 Nagarajupalli Jadoo 12 Kollapudi First class 4 Chinnagarnepudi Puja 3 Timmarajupalem Bhakthi 1 Kanchikacherla Raja 0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 51

Table 30(a): Occurrence of TSV at Bt Cotton hybrids at Guntur TSV SMW RCH 2 BG II Bunny BG II Jadoo BG II (%) PDI (%) PDI (%) PDI 36 12.97 7.5 9.34 8.0 9.57 7.5 38 9.33 6.0 9.34 5.1 9.57 5.5 40 6.69 5.0 6.33 5.0 6.05 5.0 42 1.22 2.0 6.33 2.0 6.05 2.0 44 1.01 - 1.81 - 1.76 - 46 0.81 - 1.81 - 1.76 - 48 0.81 - 1.51 - 1.76 - 50 0.81 - 1.51 - 1.37 - 52 0.81 - 1.51 - 1.37 -

Table 30(b): Verification of TSV in Cotton and weed samples Direct Antigen Coated Enzyme Linked Immunosorbent Assay (DAC-ELISA) test Guntur

S. No Sample Description DAC-ELISA reading at A405nm: 1hour Presence of TSV Cotton samples 1 Cotton leaves with very faint symptoms 0.28 +ve 2 Burnt squares, necrotic leaf margins 0.32 +ve 3 Necrosis, small leaves, purplish mottle 0.18 -ve 4 Small leaves 0.23 +ve 5 Purple spots, specks 0.29 +ve 6 Mosaic mottle 0.42 +ve 7 Purple spots, rings, patches 0.39 +ve 8 Chlorotic rings, purple mottle, speckes 0.17 -ve Weeds in cotton crop 9 Trianthema portulacportulacastrum Linn. 0.06 -ve 10 Unidentified species 0.12 -ve 11 Eclipta alba (Linn.) Hassk. 0.18 -ve 12 Cardiospermum helicacabum 0.01 -ve 13 Cyperus rotundus Linn. 0.21 +ve 14 Cleome viscosa Linn. 0.31 +ve 15 Abutilon indica Linn. 0.16 -ve 16 Tribulus terrestis Linn. 0.17 -ve 17 Parthenium hysterophorus Linn. 0.13 -ve 18 Chrozophora rottleri Linn. 0.04 -ve 19 Digera arvensis Forsk. 0.12 -ve 20 Panicum species 0.22 +ve 21 Phyllanthes neruri 0.16 -ve 22 Unidentified species 0.18 -ve 23 Cleome viscosa Linn. 0.38 +ve 24 Unidentified species 0.06 -ve 25 Unidentified species 0.09 -ve 26 Healthy Control 0.07 -ve 27 Positive Control 1.96 +ve

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 52

Table 31: Occurrence of TSV at TNAU

S. No. Location TSV (%) 1. Tirupur (RCH BGII) 3.2 2. Dharapuram (RCH BGII) 3.1 3. Ariyalur (RCH BGII) 2.2 4. Dharmapuri (RCH BGII) 2.0 5. Pottapathi (RCH BGII) 3.5 6. Erode (RCH BGII) 3.0 7. Anthiyur (RCH BGII) 2.0 8. Thondamuthur (RCH BGII) 3.8 9. Coimbatore 11.0 10. Dindigul (RCH BGII) 1.0 11. Palladam (RCH BGII) 1.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 53 Screening of breeding lines for diseases reaction

Table 32: Path 2 (a) NT: Br. 02 (a) IET- G. hirsutum (Irrigated) CLCuD(PDI) BLB (GRADE) FFLS ALB/ALS Code Entry FKT HSR SRS FKT KDW SRT CBE FKT RAH KDW SRT GUN CBE 501 ARBH-1501 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 502 TSH 321 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 4.0 1.0 503 CSH 2932 S HS HS 2.0 3.0 0.0 0.0 2.0 3.0 2.0 0.0 3.0 1.0 504 GSHV-173 HS HS HS 2.0 2.0 0.0 0.0 1.0 2.0 2.0 0.0 4.0 0.0 505 CNH 147-1 HS HS HS 2.0 1.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 506 RAH 1069 HS HS HS 2.0 2.0 0.0 0.0 2.0 3.0 2.0 0.0 3.0 1.0 507 Local Check HS HS HS 2.0 2.0 0.0 1.0 2.0 1.0 2.0 0.0 2.0 1.0 508 F 2522 HS MS HS 2.0 1.0 0.0 0.0 2.0 2.0 2.0 0.0 4.0 1.0 509 ARBH-1502 HS HS HS 2.0 2.0 0.0 1.0 2.0 1.0 2.0 0.0 3.0 2.0 510 HS 295 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 2.0 511 PBH 29 HS HS HS 2.0 3.0 0.0 1.0 2.0 2.0 2.0 0.0 4.0 1.0 512 RB-602 HS HS HS 2.0 1.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 513 CPD-1501 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 Z C (H 1300/ CNHO 514 HS HS HS 2.0 2.0 0.0 1.0 2.0 2.0 2.0 0.0 3.0 0.0 12/Suraj) 515 SHM-55 HS HS HS 2.0 3.0 0.0 1.0 2.0 2.0 2.0 0.0 3.0 1.0 516 CSH 2920 HS HS S 2.0 2.0 0.0 0.0 2.0 3.0 2.0 0.0 - 0.0 517 BGDS 1055 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 2.0 1.0 518 F 2532 HS S HS 2.0 3.0 0.0 0.0 2.0 1.0 2.0 0.0 2.0 0.0 519 CNH 126 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 520 GJHV-517 HS HS HS 2.0 1.0 0.0 0.0 2.0 0.0 2.0 0.0 3.0 0.0 521 H 1478 HS HS HS 2.0 2.0 0.0 1.0 2.0 1.0 2.0 0.0 3.0 1.0 522 RS 2815 HS HS HS 2.0 2.0 0.0 0.0 2.0 1.0 2.0 0.0 2.0 1.0 Q C (F 523 HS HS HS 2.0 2.0 0.0 0.0 2.0 1.0 2.0 0.0 2.0 2.0 2164/Suraj/Suraj) 524 RAH 1271 HS HS HS 2.0 2.0 0.0 0.0 2.0 1.0 2.0 0.0 3.0 1.0 525 CPD-1502 HS HS HS 2.0 2.0 0.0 0.0 2.0 3.0 2.0 0.0 3.0 2.0 526 TCH 1716 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 0.0 527 CCH 15-1 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 528 HS 296 HS HS HS 2.0 1.0 0.0 0.0 2.0 3.0 2.0 0.0 4.0 1.0 529 GSHV-172 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 530 TSH 322 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 4.0 0.0 531 PBH 21 HS HS HS 2.0 2.0 0.0 0.0 2.0 3.0 2.0 0.0 3.0 1.0 532 BGDS 1033 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 3.0 0.0 3.0 2.0 533 GJHV-519 HS HS HS 2.0 2.0 0.0 0.0 2.0 1.0 2.0 0.0 3.0 0.0 534 RS 2797 HS HS HS 2.0 2.0 0.0 1.0 2.0 1.0 2.0 1.0 3.0 1.0 535 TCH 1824 HS HS HS 2.0 1.0 0.0 1.0 2.0 1.0 2.0 1.0 3.0 1.0 536 L – 799 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 3.0 0.0 1.0 1.0 537 RB-601 HS HS HS 2.0 2.0 0.0 0.0 2.0 2.0 2.0 0.0 3.0 1.0 538 CCH 15-2 HS HS HS 2.0 3.0 0.0 0.0 1.0 1.0 2.0 0.0 3.0 1.0 SC*-RST9 (FDK) HS 3.0 2.0 SC-HS6 (HSR) HS SC-HS6 (SRS) HS SC-RCHBGII (CBE) 1.0 2.0 SC-RCH2BGII (GUN) 3.0 SC-JadooBGII (GUN) 3.0 SC-LRA5166 (SRT) 3.0 SC-LRA5166 (RAH) 3.0 SC-JK4 (KDW) 3.0 2.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 54 Table 32: Contd..

GM RUST MLB Root rot% TSV% Code Entry KDW GUN GUN KDW CBE CBE 501 ARBH-1501 3.0 1.0 3.0 2.0 5.0 0.0 502 TSH 321 2.0 3.0 2.0 2.0 0.0 0.0 503 CSH 2932 2.0 1.0 3.0 2.0 0.0 0.0 504 GSHV-173 2.0 3.0 3.0 2.0 0.0 0.0 505 CNH 147-1 2.0 3.0 3.0 3.0 6.0 0.0 506 RAH 1069 2.0 3.0 3.0 2.0 0.0 0.0 507 Local Check 2.0 3.0 3.0 3.0 0.0 3.0 508 F 2522 2.0 4.0 3.0 2.0 0.0 0.0 509 ARBH-1502 2.0 2.0 2.0 3.0 10.0 3.0 510 HS 295 3.0 2.0 3.0 2.0 0.0 0.0 511 PBH 29 2.0 3.0 3.0 3.0 0.0 3.0 512 RB-602 2.0 3.0 3.0 2.0 0.0 0.0 513 CPD-1501 3.0 3.0 2.0 3.0 0.0 0.0 514 Z C (H 1300/ CNHO 12/Suraj) 3.0 1.0 2.0 2.0 0.0 3.0 515 SHM-55 2.0 2.0 3.0 3.0 6.0 3.0 516 CSH 2920 2.0 - - 3.0 0.0 0.0 517 BGDS 1055 2.0 3.0 3.0 2.0 6.0 0.0 518 F 2532 2.0 2.0 3.0 3.0 10.0 0.0 519 CNH 126 2.0 3.0 3.0 2.0 0.0 0.0 520 GJHV-517 2.0 2.0 2.0 3.0 0.0 0.0 521 H 1478 2.0 1.0 3.0 2.0 0.0 5.0 522 RS 2815 2.0 3.0 3.0 2.0 0.0 0.0 523 Q C (F 2164/Suraj/Suraj) 2.0 3.0 3.0 2.0 0.0 0.0 524 RAH 1271 3.0 2.0 3.0 2.0 0.0 0.0 525 CPD-1502 2.0 2.0 3.0 2.0 5.0 0.0 526 TCH 1716 2.0 0.0 3.0 3.0 0.0 0.0 527 CCH 15-1 3.0 2.0 3.0 3.0 0.0 0.0 528 HS 296 2.0 2.0 2.0 2.0 0.0 0.0 529 GSHV-172 2.0 2.0 3.0 2.0 5.0 0.0 530 TSH 322 2.0 2.0 3.0 3.0 0.0 0.0 531 PBH 21 2.0 3.0 2.0 2.0 5.0 0.0 532 BGDS 1033 2.0 1.0 2.0 2.0 0.0 0.0 533 GJHV-519 2.0 3.0 3.0 2.0 0.0 0.0 534 RS 2797 2.0 1.0 2.0 2.0 0.0 6.0 535 TCH 1824 2.0 2.0 3.0 3.0 0.0 3.0 536 L – 799 2.0 0.0 4.0 2.0 0.0 0.0 537 RB-601 2.0 2.0 3.0 2.0 0.0 0.0 538 CCH 15-2 2.0 3.0 2.0 2.0 0.0 0.0 SC*-RST9 (FDK) SC-HS6 (HSR) SC-HS6 (SRS) SC-RCH BGII (CBE) 0.0 6.0 SC-RCH2 BGII (GUN) 1.0 4.0 SC-Jadoo BGII (GUN) 4.0 4.0 SC-LRA 5166 (SRT) SC-LRA 5166 (RAH) SC-JK4 (KDW) 2.0 3.0 *SC-Susceptible check

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 55

Table 33: Br. 02 b – NT IET- G. hirsutum (Rainfed)

AB BB GM Rust Code Entry DWD DWD DWD DWD 551 RAH 1271 3.0 2.0 2.0 4.0 552 CNH07-16 4.0 1.0 2.0 3.0 553 AKH-09-5 3.0 1.0 3.0 4.0 554 BGDS 1033 2.0 1.0 1.0 4.0 555 CCH 15-3 3.0 1.0 3.0 4.0 556 Q C (Suraj) 4.0 2.0 2.0 3.0 557 CNH 1123 4.0 1.0 2.0 4.0 558 CPD-1551 4.0 2.0 2.0 3.0 559 ARBH-1551 4.0 2.0 2.0 4.0 560 NDLH – 2028-2 3.0 1.0 2.0 4.0 561 CNH 1122 4.0 2.0 2.0 4.0 562 BGDS 1055 4.0 2.0 2.0 3.0 563 RAH 1069 4.0 1.0 2.0 3.0 564 CPD-1552 4.0 2.0 2.0 4.0 565 Local Check 4.0 2.0 1.0 4.0 566 NDLH – 2005-4 4.0 1.0 2.0 4.0 567 GBHV-198 4.0 2.0 1.0 3.0 568 ARBH-1552 3.0 2.0 2.0 4.0 569 H 1464 2.0 2.0 2.0 3.0 570 PH 1070 3.0 2.0 1.0 4.0 571 Z C (NH 615/ Sahana) 4.0 2.0 2.0 4.0 572 CNH09-9 3.0 1.0 2.0 4.0 573 IH 11-10 3.0 2.0 2.0 4.0 574 GBHV-195 3.0 1.0 2.0 3.0 575 CCH 15-4 2.0 2.0 2.0 2.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 56

Table 34: Br. 05 (a) NT-(PHT) Intra hirsutum-Hybrid CLCuD(PDI) BLB (GRADE) FFLS ALB/ALS GM RUST Root rot% Code Entry FKT HSR FKT SRT CBE FKT RAH SRT GUN CBE CBE GUN GUN CBE 601 MRC 7399 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 2.0 1.0 0.0 3.0 2.0 0.0 602 ARBHH-1501 HS HS 2.0 0.0 1.0 2.0 3.0 0.0 3.0 1.0 1.0 3.0 3.0 0.0 603 LAHH 29 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 3.0 1.0 1.0 3.0 3.0 0.0 604 25D14 MS HS 2.0 3.0 0.0 2.0 1.0 0.0 2.0 1.0 1.0 1.0 2.0 5.0 605 ACH-152 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 3.0 1.0 0.0 2.0 3.0 0.0 606 FHH 269 HS HS 2.0 0.0 1.0 2.0 2.0 0.0 3.0 1.0 0.0 2.0 2.0 0.0 607 BGDHH 632 HS HS 2.0 0.0 0.0 2.0 0.0 0.0 2.0 1.0 1.0 2.0 3.0 0.0 608 RAHH 630 HS S 2.0 0.0 0.0 2.0 3.0 0.0 3.0 1.0 0.0 4.0 3.0 0.0 609 GTHH-217 HS S 2.0 0.0 0.0 2.0 1.0 0.0 4.0 1.0 0.0 4.0 3.0 0.0 610 RHH-1215 HS MS 2.0 0.0 0.0 2.0 2.0 0.0 3.0 1.0 0.0 3.0 3.0 0.0 611 DHH-1501 HS HS 2.0 0.0 0.0 2.0 3.0 0.0 2.0 1.0 0.0 2.0 3.0 5.0 612 BCHH 7588 HS HS 2.0 0.0 0.0 2.0 3.0 0.0 3.0 1.0 0.0 3.0 3.0 0.0 613 GJHH-7 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 2.0 1.0 0.0 4.0 3.0 0.0 614 Z C (CSHH 198/ Ankur 651/ Bunny) HS S 2.0 0.0 0.0 2.0 2.0 0.0 2.0 1.0 0.0 3.0 3.0 0.0 615 FHH 272 HS S 2.0 0.0 0.0 2.0 2.0 0.0 3.0 1.0 0.0 3.0 3.0 0.0 616 RAHH 671 HS HS 2.0 0.0 0.0 2.0 3.0 0.0 3.0 1.0 1.0 3.0 2.0 0.0 617 CSHG 2911 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 3.0 1.0 0.0 2.0 3.0 0.0 618 GSHH-2737 HS HS 2.0 0.0 0.0 2.0 2.0 0.0 2.0 1.0 0.0 3.0 3.0 0.0 619 HHH 496 HS S 2.0 0.0 0.0 2.0 2.0 0.0 3.0 1.0 0.0 3.0 2.0 0.0 620 RHH-1121 HS HS 2.0 0.0 0.0 2.0 2.0 0.0 2.0 1.0 1.0 3.0 2.0 0.0 621 Local Check HS HS 2.0 0.0 1.0 2.0 1.0 0.0 3.0 1.0 0.0 3.0 2.0 0.0 622 Ankur 4252 HS HS 2.0 0.0 0.0 2.0 2.0 0.0 3.0 1.0 0.0 3.0 3.0 0.0 623 GJHH-5 HS HS 2.0 0.0 0.0 2.0 1.0 0.0 3.0 1.0 1.0 3.0 2.0 0.0 624 GSGHH-630 HS HS 2.0 0.0 0.0 2.0 3.0 0.0 3.0 1.0 0.0 3.0 3.0 0.0 625 LAHH 28 HS HS 2.0 0.0 0.0 2.0 4.0 0.0 3.0 1.0 0.0 3.0 4.0 0.0 626 BGDHH 697 HS HS 2.0 0.0 0.0 2.0 2.0 0.0 3.0 1.0 1.0 3.0 3.0 0.0 SC-RST9 (FDK) HS 3.0 2.0 SC-HS6 (HSR) HS SC-Jadoo BGII (GUN) 4.0 4.0 4.0 SC-RCH BGII (CBE) 0.0 1.0 0.0 0.0 SC-LRA5166(RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 57

Table 35: Br. 05 b NT-PHT- Intra hirsutum-Hybrid

AB BB GM Rust Code Entry DWD DWD DWD DWD 651 BGDHH 697 4.0 2.0 1.0 3.0 652 RAHH 630 4.0 2.0 2.0 4.0 653 ACH-151 3.0 1.0 2.0 3.0 654 DHH-1551 3.0 2.0 1.0 3.0 655 GTHH-215 3.0 1.0 2.0 4.0 656 NHH 719 4.0 1.0 1.0 3.0 657 ARBHH-1551 4.0 2.0 2.0 4.0 658 Zonal Check (Ankur 651/ Bunny) 4.0 2.0 1.0 3.0 659 NCS 5657 3.0 1.0 2.0 3.0 660 BGDHH 632 3.0 1.0 2.0 4.0 661 RAHH 671 4.0 2.0 2.0 3.0 662 CAHH-275 3.0 2.0 2.0 4.0 663 Local Check 4.0 2.0 2.0 4.0 664 DHH-1552 4.0 2.0 2.0 4.0 665 ARBHH-1552 4.0 2.0 2.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 58 Table 36: Br. 06 a -NT – IET of Compact genotypes under irrigated condition CLCuD (PDI) BLB (GRADE) ALB/ALS GM RUST Root rot% code entry FKT HSR CBE RAH GUN CBE CBE GUN GUN CBE 701 RAHC 1019 HS HS 0.0 2.0 3.0 1.0 0.0 2.0 3.0 0.0 702 CNH 140-1 HS HS 0.0 3.0 3.0 1.0 0.0 1.0 2.0 5.0 703 CSH-5038 HS HS 1.0 1.0 3.0 1.0 0.0 2.0 2.0 0.0 704 ARBC 1501 HS HS 0.0 2.0 3.0 1.0 1.0 3.0 2.0 0.0 705 RS 2814 MS HS 0.0 3.0 3.0 1.0 0.0 3.0 2.0 0.0 706 CCH 15-5 HS S 0.0 3.0 4.0 2.0 0.0 2.0 2.0 0.0 707 ANGC 1501 HS HS 0.0 2.0 4.0 1.0 0.0 2.0 2.0 0.0 708 CSH-6109 HS HS 0.0 2.0 3.0 1.0 0.0 3.0 2.0 0.0 709 DSC-1501 HS HS 0.0 3.0 3.0 1.0 0.0 3.0 2.0 0.0 710 GSHV 171 HS S 1.0 3.0 3.0 1.0 1.0 4.0 3.0 0.0 711 L C (Closer spacing) HS S 1.0 2.0 3.0 1.0 1.0 2.0 2.0 0.0 712 TCH 1876 HS S 0.0 1.0 3.0 1.0 0.0 2.0 2.0 0.0 713 PBH 19 HS S 0.0 1.0 3.0 1.0 0.0 2.0 2.0 0.0 714 LHDP 1 HS HS 1.0 2.0 4.0 1.0 0.0 3.0 2.0 5.0 715 CNH 95-4 HS HS 0.0 2.0 4.0 1.0 0.0 2.0 2.0 5.0 716 GTHV-13/28 HS HS 1.0 3.0 3.0 1.0 0.0 3.0 2.0 0.0 717 ANGC 1502 HS HS 1.0 1.0 4.0 1.0 0.0 4.0 2.0 0.0 718 ARBC 1502 HS HS 1.0 2.0 4.0 1.0 0.0 2.0 2.0 5.0 719 DSC 1502 HS HS 0.0 3.0 4.0 1.0 0.0 3.0 2.0 10.0 720 CCH 15-6 HS HS 0.0 3.0 4.0 1.0 0.0 2.0 3.0 0.0 721 H 1490 S HS 0.0 2.0 4.0 1.0 0.0 3.0 2.0 5.0 722 RAHC 1017 HS S 0.0 1.0 4.0 1.0 0.0 2.0 3.0 0.0 723 CSH-3158 HS S 0.0 3.0 3.0 1.0 0.0 3.0 3.0 0.0 724 RS 2821 MS HS 0.0 3.0 3.0 1.0 0.0 3.0 3.0 0.0 SC-Rch 650 (FDK) MS SC-HS6 (HSR) HS SC-Jadoo BGII (GUN) 3.0 4.0 4.0 SC-RCH BGII (CBE) 0.0 1.0 0.0 0.0 SC-LRA 5166 (RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 59

Table 37: NT- Br. 6 (b) IET of compact genotypes (Rainfed)

BLB ALB/ALS GM RUST Code Entry SRT DWD SRT DWD DWD DWD 731 RAHC 1017 0.0 2.0 0.0 4.0 1.0 4.0 732 CNH 09-7 0.0 2.0 0.0 3.0 1.0 3.0 733 CNH 20 0.0 2.0 0.0 3.0 2.0 3.0 734 LHDP 1 0.0 3.0 0.0 4.0 2.0 4.0 735 GSHV-180 0.0 2.0 0.0 4.0 1.0 4.0 736 Local Check (Closer spacing) 0.0 2.0 0.0 4.0 2.0 4.0 737 DSC 1552 0.0 2.0 0.0 4.0 2.0 3.0 738 CNH 1560 0.0 1.0 0.0 3.0 1.0 4.0 739 ARBC 1551 0.0 1.0 0.0 3.0 1.0 3.0 740 CNH 131 0.0 - 0.0 - - - 741 GTHV-13/32 0.0 2.0 0.0 4.0 2.0 4.0 742 GISV -272 0.0 2.0 0.0 4.0 2.0 4.0 743 CCH 15-7 0.0 2.0 0.0 3.0 1.0 4.0 744 CNH 09-5 0.0 1.0 0.0 4.0 2.0 4.0 745 RAHC 1019 0.0 2.0 0.0 4.0 2.0 4.0 746 CNH 1124 0.0 1.0 0.0 4.0 1.0 4.0 747 CCH 15-8 0.0 1.0 0.0 3.0 1.0 4.0 748 DSC-1551 0.0 2.0 0.0 3.0 2.0 4.0 749 AKH-780 0.0 2.0 0.0 3.0 1.0 4.0 SC-Abhadita(DWD) 4.0 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 60

Table 38: NT-Br 12 (a) IET OF G. barbadense ALB/ALS BLB GM RUST TSV% Code Entry RAH SRT GUN DWD CBE SRT DWD CBE DWD GUN CBE DWD GUN CBE 751 DB-1502 1.0 0.0 3.0 2.0 1.0 0.0 1.0 0.0 2.0 0.0 0.0 3.0 1.0 0.0 752 ARBB-1501 1.0 0.0 2.0 2.0 1.0 0.0 2.0 0.0 2.0 0.0 0.0 3.0 1.0 0.0 753 CCB-11a 1.0 0.0 2.0 3.0 1.0 0.0 1.0 0.0 2.0 0.0 0.0 4.0 2.0 6.0 754 DB-1501 1.0 0.0 3.0 2.0 - 0.0 2.0 0.0 2.0 3.0 0.0 3.0 1.0 - 7555 Suvin (CC) 1.0 0.0 3.0 2.0 1.0 0.0 1.0 0.0 2.0 0.0 0.0 2.0 1.0 0.0 756 ARBB-1502 2.0 0.0 2.0 2.0 1.0 0.0 2.0 0.0 1.0 0.0 0.0 3.0 2.0 0.0 757 CCB-44 NG 0.0 - - 1.0 0.0 - 0.0 - - 0.0 - - 0.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0 SC-RCH BGII (CBE) 1.0 0.0 0.0 6.0 SC-LRA 5166 (RAH) 3.0

Table 39: NT-Br. 15 a - PHT- Interspecific -Hybrid (hir x barb) ALB/ALS BLB GM RUST Root rot % TSV% Code Entry RAH GUN DWD CBE DWD CBE DWD GUN CBE DWD GUN CBE CBE 761 RHB-1243 2.0 3.0 3.0 1.0 1.0 0.0 2.0 0.0 0.0 3.0 3.0 0.0 2.0 762 DHB-1501 1.0 2.0 3.0 1.0 2.0 0.0 2.0 0.0 0.0 4.0 3.0 0.0 3.0 763 ARBHB-1502 0.0 3.0 3.0 1.0 1.0 0.0 2.0 0.0 0.0 3.0 3.0 0.0 20 764 Ankur Nandini 0.0 3.0 3.0 1.0 2.0 0.0 2.0 0.0 0.0 4.0 3.0 0.0 4.0 765 ARBHB-1501 1.0 3.0 4.0 1.0 2.0 0.0 1.0 0.0 0.0 3.0 3.0 0.0 2.0 766 CCHB-54 0.0 3.0 3.0 1.0 1.0 0.0 1.0 0.0 0.0 4.0 3.0 0.0 3.0 767 Local Check 2.0 2.0 3.0 1.0 2.0 0.0 2.0 2.0 0.0 4.0 3.0 0.0 4.0 768 DCH 32 (CC) 1.0 3.0 4.0 1.0 2.0 0.0 2.0 0.0 0.0 4.0 4.0 0.0 2.0 769 DHB-1502 1.0 3.0 4.0 2.0 1.0 0.0 1.0 0.0 0.0 4.0 3.0 0.0 6.0 770 RHB-1008 3.0 3.0 4.0 1.0 2.0 0.0 2.0 0.0 0.0 4.0 3.0 0.0 2.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0 SC-JadooBGII (GUN) 3.0 4.0 4.0 SC-RCHBGII (CBE) 1.0 0.0 0.0 0.0 4.0 SC-LRA5166 (RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 61 Table 40: NT-Br22a/b IET-G. arboreum BLB (GRADE) FFLS ALB/ALS GM RUST MLB Root rot Code Entry KDW FKT FKT KDW DWD KDW DWD DWD KDW SRS 801 JLA-0804 2.0 1.0 2.0 2.0 2.0 3.0 4.0 2.0 2.0 S 802 GAM-231 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S 803 PA 801 1.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 804 CNA-2023 2.0 1.0 3.0 2.0 2.0 2.0 4.0 2.0 3.0 MR 805 ARBa-1502 2.0 1.0 3.0 2.0 2.0 2.0 4.0 2.0 3.0 S 806 CNA 2019 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S 807 RG 784 1.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 808 CSA 1028 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S 809 Z C (FDK 124/AKA 7/DLSa 17) 1.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 810 FDK 260 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 811 ARBa-1501 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 812 PBD 6 1.0 1.0 1.0 2.0 2.0 2.0 4.0 2.0 3.0 S 813 PA 812 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 814 NDLA - 3089 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 - 815 CSA 2043 2.0 1.0 2.0 2.0 2.0 3.0 4.0 2.0 3.0 S 816 DWDa-1501 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S 817 CCA-2003 1.0 1.0 1.0 2.0 2.0 1.0 4.0 2.0 3.0 S 818 AKA-209 2.0 1.0 2.0 2.0 2.0 3.0 4.0 2.0 3.0 S 819 FDK 259 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 820 Local Check 1.0 1.0 2.0 2.0 1.0 2.0 4.0 1.0 2.0 S 821 DWDa-1502 2.0 1.0 2.0 2.0 2.0 2.0 4.0 1.0 2.0 S 822 RG 778 2.0 1.0 3.0 2.0 2.0 2.0 4.0 1.0 3.0 S 823 NDLA - 3094 2.0 1.0 2.0 2.0 1.0 2.0 4.0 1.0 2.0 S 824 CISA 6-123 2.0 1.0 2.0 3.0 1.0 2.0 4.0 1.0 2.0 S 825 GAM-235 3.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 3.0 S 826 PBD 17 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S 827 HD 524 1.0 1.0 2.0 2.0 2.0 1.0 4.0 2.0 3.0 S 828 JLA-0906 2.0 1.0 2.0 2.0 2.0 2.0 4.0 2.0 2.0 S SC-CISA310 (SRS) S SC-F124 (FDK) 1.0 1.0 SC-Abhadita (DWD) 4.0 4.0 4.0 SC-JK4 (KDW) 3.0 2.0 3.0 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 62

Table 41: NT- Br. 25 (a/b): PHT of Desi cotton

BLB ALS/ALB FFLS Root rot Code Entry SRT FDK SRT FDK SRS 851 CISAA 14-32 0.0 1.0 0.0 2.0 S 852 GSGDH-521 0.0 1.0 0.0 2.0 S 853 Zonal Check (CISAA 2-NZ; NACH 12-CZ) 0.0 1.0 0.0 1.0 - 854 AKDH-101 0.0 1.0 0.0 1.0 S 855 CISAA 14-31 0.0 2.0 0.0 2.0 S 856 NACH-436 0.0 1.0 0.0 2.0 S 857 Local Check 0.0 1.0 0.0 2.0 - 858 BDAA 011 0.0 2.0 0.0 2.0 MS 859 AAH 37 0.0 1.0 0.0 1.0 S 860 Swadeshi 9 0.0 1.0 0.0 2.0 S SC-CISA310 (SRS) S SC-F124 (FDK) 1.0 2.0 SC-LRA5166 (SRT) 3.0 0.0

Table 42: NT-Br. 32 (b): IET of G. herbaceum cottons BLB ALS/ALB Wilt GM RUST Code Entry SRT SRT DWD SRT DWD DWD 861 GShv-332/11 0.0 0.0 2.0 0.0 4.0 1.0 862 GBhv- 297 0.0 0.0 2.0 0.0 4.0 1.0 863 RAHS 813 0.0 0.0 3.0 0.0 4.0 1.0 864 Local Check 0.0 0.0 3.0 0.0 4.0 1.0 865 GShv-293/11 1.0 0.0 2.0 0.0 4.0 2.0 866 GShv-280/11 1.0 0.0 2.0 0.0 4.0 1.0 867 GShv-300/11 1.0 0.0 2.0 0.0 4.0 1.0 868 GBhv- 299 0.0 0.0 3.0 0.0 4.0 1.0 869 GVhv-655 1.0 0.0 2.0 0.0 4.0 1.0 870 RAHS 814 1.0 0.0 2.0 0.0 4.0 1.0 871 RAHS 812 1.0 0.0 2.0 0.0 4.0 1.0 872 ANGh-1502 1.0 0.0 2.0 0.0 4.0 1.0 873 Zonal Check (G Cot 23/DDhc 11) 1.0 0.0 3.0 0.0 4.0 2.0 874 ANGh-1501 1.0 0.0 3.0 0.0 4.0 1.0 875 DWDh-1501 1.0 0.0 3.0 0.0 4.0 2.0 876 RAHS 811 1.0 0.0 2.0 0.0 4.0 1.0 877 DWDh-1502 1.0 0.0 3.0 0.0 4.0 1.0 878 2388/99 1.0 0.0 2.0 0.0 4.0 1.0 879 GBhv- 301 1.0 0.0 2.0 0.0 4.0 1.0 880 GBhv- 300 1.0 0.0 3.0 0.0 4.0 2.0 SC-Abhadita (DWD 4.0 4.0 4.0 SC-LRA5166 (SRT) 3.0 0.0 0.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 63 ZONAL TRIAL (NORTH ZONE)

Table 43: NZ Br-03 (a)-Screening of G. hirsutum entries against CLCuD and other diseases BLB CLCuD(PDI) FFLS Code Entry (GRADE) FKT BHT HSR SNG FKT BHT FKT BHT 5001 CSH 3088 HS HS S HS 2.0 1.0 2.0 1.0 5002 RS 2765 HS S S HS 2.0 0.0 2.0 0.0 5003 Q C (F 2164) HS S S HS 2.0 0.0 2.0 0.0 5004 LH 2232 HS HS HS S 1.0 1.0 1.0 1.0 5005 Z C (H 1300) HS MS S HS 2.0 0.0 2.0 0.0 5006 Su-Flum HS HS HS S 2.0 0.0 2.0 0.0 5007 F 2501 HS HS HS MS 2.0 0.0 2.0 0.0 5008 HS 294 HS HS HS HS 2.0 2.0 2.0 2.0 5009 F 2296 HS HS HS S 1.0 0.0 2.0 0.0 5010 Local Check HS S S S 2.0 0.0 2.0 0.0 5011 PBH 13 HS HS S MS 2.0 1.0 2.0 2.0 5012 CSH 3232 HS HS HS HS 2.0 0.0 2.0 0.0 Shakti Sultan 5013 HS HS HS S 2.0 0.0 2.0 1.0 (SSGR105) SC-RST9 (FDK) HS 2.0 2.0 SC-F846 (BHT) HS 2.0 2.0 SC-HS6 (HSR) HS SC-RS921 (SNG) HS

Table 44: NZ Br. 05 (a) (CHT) CLCuD(PDI) BLB (GRADE) FFLS Code Entry FKT BHT HSR SNG SRS FKT BHT FKT BHT 5021 HHH 495 HS MS HS HS HS 2.0 0.0 2.0 0.0 5022 25D11 HS S HS S HS 2.0 0.0 2.0 0.0 5023 HSHH 32 HS S HS S HS 2.0 0.0 2.0 2.0 5024 FHH 261 HS S HS S HS 2.0 0.0 2.0 0.0 5025 GTHH 215 HS MS S S HS 2.0 0.0 2.0 0.0 5026 HHH 494 HS S S S HS 2.0 0.0 2.0 0.0 5027 Local Check HS MS S HS HS 2.0 0.0 2.0 0.0 5028 Zonal Check (CSHH198) HS HS HS S HS 2.0 2.0 2.0 2.0 5029 CSHH-2012 HS HS HS HS HS 2.0 2.0 2.0 2.0 SC-RST9 (FDK) HS 3.0 2.0 SC-F846 (BHT) HS 2.0 2.0 SC-HS6 (HSR) HS SC-RS921 (SNG) HS SC-HS6 (SRS) HS

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 64 Table 45: NZ Br. 06 (a) CLCuD(PDI) BLB (GRADE) FFLS Code Entry FKT BHT HSR SNG SRS BHT BHT 5031 RS 2718 S HS S HS HS 1.0 2.0 5032 CSH 73 HS HS S HS HS 2.0 2.0 5033 RS 2727 S S HS S S 0.0 0.0 5034 L C (Closer spacing) HS MR S HS HS 0.0 0.0 5035 PBH 3 HS S S HS HS 0.0 0.0 5036 CSH 3113 HS HS HS HS HS 0.0 2.0 5037 RS 2734 MS S HS HS HS 0.0 0.0 5038 CSH 3178 HS S HS HS HS 0.0 0.0 5039 H 1465 HS HS HS HS HS 0.0 2.0 SC-RCH650 (FDK) MS SC-F846 (BHT) HS 2.0 2.0 SC-HS6 (HSR) HS SC-RS921 (SNG) HS SC-HS6 (SRS) HS

Table 46: NZ Br. 24 (a) BLB (GRADE) FFLS Root rot Code Entry FKT BHT FKT BHT SRS 5041 LD 1026 1.0 0.0 1.0 0.0 S 5042 HD 514 1.0 0.0 2.0 0.0 S 5043 Zonal Check (FDK 124) 1.0 0.0 2.0 0.0 S 5044 CISA 6-2 1.0 0.0 2.0 0.0 S 5045 Local Check 1.0 0.0 1.0 0.0 MS 5046 RG 776 1.0 0.0 2.0 0.0 S 5047 LD 1019 1.0 0.0 2.0 0.0 S SC-LD949 (FDK) 1.0 2.0 SC-F846 (BHT) 2.0 2.0 SC-CISA310 (SRS) S

CENTRAL ZONE

Table 47: CZ Br 03 (a) Preliminary varietal trial of G. hirsutum ALB BLB Code Entry RAH JNG SRT JNG 5101 GJHV 497 2.0 3.0 0.0 1.0 5102 Quality Check (Suraj) 2.0 3.0 1.0 1.0 5103 SCS 1061 1.0 3.0 1.0 1.0 5104 CCH 14-1 2.0 3.0 1.0 1.0 5105 Z C (CNHO 12) 2.0 3.0 0.0 1.0 5106 Local Check 3.0 3.0 0.0 1.0 5107 TCH 1806 3.0 3.0 0.0 1.0 5108 GJHV 510 2.0 3.0 0.0 1.0 SC-LRA 5166 (JNG) 3.0 2.0 SC-LRA 5166 (RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 65 Table 48: CZ Br- 04 (a) ALB BLB Code Entry RAH JNG SRT JNG 5111 CCH 12-2 2.0 3.0 0.0 1.0 5112 BGDS 1063 1.0 3.0 0.0 1.0 5113 Quality Check (Suraj) 1.0 3.0 1.0 1.0 5114 GJHV 514 0.0 3.0 0.0 1.0 5115 TCH 1777 2.0 3.0 0.0 1.0 5116 GSHV-164 1.0 3.0 0.0 1.0 5117 Zonal Check (CNHO 12) 2.0 3.0 0.0 1.0 5118 CCH 11-2 2.0 3.0 0.0 1.0 5119 Local Check 3.0 3.0 1.0 1.0 5120 GJHV-516 1.0 3.0 0.0 1.0 5121 P 5430 2.0 3.0 1.0 1.0 SC-LRA 5166 (JNG) 3.0 2.0 SC-LRA 5166 RAH) 3.0

Table 49: CZ Br- 05 (a) ALB BLB MLB GM Code Entry RAH KDW KDW SRT KDW KDW 5131 RHH-1015 2.0 - - 0.0 - - 5132 SHH 818 1.0 2.0 2.0 1.0 2.0 1.0 5133 GTHH 215 2.0 2.0 2.0 1.0 2.0 1.0 5134 RHH 1125 3.0 2.0 2.0 0.0 2.0 2.0 5135 NCS 5657 1.0 2.0 3.0 0.0 2.0 2.0 5136 MRC 7388 2.0 2.0 2.0 0.0 2.0 2.0 5137 Local Check 2.0 2.0 3.0 0.0 2.0 2.0 5138 GJHH 4 1.0 2.0 2.0 0.0 2.0 2.0 5139 Zonal Check (Ankur 651) 2.0 2.0 3.0 1.0 2.0 2.0 5140 RHH 1112 2.0 2.0 2.0 1.0 2.0 1.0 5141 RHH-1007 3.0 2.0 2.0 0.0 3.0 2.0 SC-LRA 5166 (SRT) 3.0 SC-LRA 5166 (RAH) 3.0 SC-JK4 (KDW) 2.0 3.0 3.0 2.0

Table 50: CZ Br- 06 (a) ALB Code Entry RAH 5151 GISV 272 3.0 5152 Local Check (Coded & Closer spacing) 2.0 5153 ANGC 1402 2.0 5154 GSHV 180 2.0 5155 CCH 12-6 3.0 5156 ARBC-1301 3.0 5157 RAHC 1011 1.0 5158 GTHV 13/32 2.0 SC-LRA 5166 (RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 66

Table 51: CZ Br- 13 (a)

ALB BB Code Entry RAH SRT 5161 DB 1402 1.0 - 5162 CCB 36 0.0 - 5163 Zonal Check (Suvin) 0.0 0.0 5164 ARBB 1401 1.0 0.0 5165 CCB 29 1.0 0.0 5166 ARBB 1402 1.0 0.0 SC-LRA 5166 (RAH) 3.0

Table 52: CZ Br- 14 (a)

ALB Code Entry RAH 5171 ARBB-1302 2.0 5172 GSB-44 2.0 5173 Zonal Check (Suvin) 1.0 5174 DB-1301 2.0 5175 GSB 43 1.0 5176 TCB 37 1.0 SC-LRA 5166 (RAH) 3.0

Table 53: CZ Br- 15 (a)

ALB Code Entry RAH 5181 RHB 1122 2.0 5182 Zonal Check (DCH 32) 1.0 5183 ARBHB 1401 1.0 5184 DHB 1009 2.0 5185 RHB 1123 1.0 5186 DHB-1301 0.0 5187 Local Check 0.0 5188 RHB-1014 1.0 5189 DHB 1012 0.0 SC-LRA 5166 (RAH) 3.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 67 Table 54: CZ Br03 (b) ALB BB GM wilt MLS Code Entry NAD SRT NAD AKL SRT NAD AKL SRT AKL 5201 SCS 1061 2.0 1.0 0.0 1.0 0.0 0.0 1.0 0.0 1.0 5202 GBHV 183 3.0 0.0 0.0 2.0 0.0 0.0 1.0 0.0 1.0 5203 Local Check 2.0 1.0 0.0 2.0 1.0 0.0 1.0 0.0 2.0 5204 GTHV 13/17 2.0 0.0 2.0 2.0 0.0 0.0 1.0 0.0 1.0 5205 IH 11 2.0 1.0 1.0 1.0 1.0 0.0 1.0 0.0 1.0 5206 Quality Check (Suraj) 2.0 0.0 2.0 2.0 2.0 0.0 1.0 0.0 2.0 5207 Zonal Check (NH 615) 2.0 1.0 0.0 1.0 0.0 0.0 1.0 0.0 1.0 5208 SCS 1207 2.0 2.0 2.0 1.0 1.0 0.0 1.0 0.0 1.0 5209 RAH 1066 3.0 0.0 0.0 2.0 0.0 0.0 1.0 0.0 2.0 SC-LRA 5166 (NAD) 3.0 2.0 1.0 SC-LRA 5166 Bharuch 2.0 1.0 0.0 SC-LRA 5166 (AKL) 2.0 1.0 1.0 SC-LRA 5166 (SRT) 0.0 3.0 0.0

Table 55: CZ Br04 (b) ALB BB GM wilt MLS Code Entry NAD SRT NAD AKL SRT NAD AKL SRT AKL 5211 BGDS 1063 2.0 1.0 2.0 2.0 0.0 0.0 1.0 0.0 1.0 5212 Zonal Check (NH 615) 2.0 0.0 2.0 2.0 0.0 0.0 1.0 0.0 1.0 5213 CCH 12-3 2.0 1.0 1.0 1.0 1.0 0.0 1.0 0.0 1.0 5214 Local Check 2.0 1.0 1.0 2.0 0.0 0.0 1.0 0.0 1.0 5215 Quality Check (Suraj) 2.0 0.0 1.0 2.0 0.0 0.0 1.0 0.0 1.0 SC-LRA 5166 (NAD) 3.0 2.0 1.0 SC-LRA 5166 Bharuch 2.0 1.0 0.0 SC-LRA 5166 (AKL) 2.0 2.0 1.0 SC-LRA 5166 (SRT) 3.0 0.0

Table 56: CZ Br05 (b) ALB BB GM wilt MLS Code Entry NAD SRT NAD AKL SRT NAD AKL SRT AKL 5221 SHH 808 2.0 1.0 2.0 2.0 1.0 0.0 1.0 0.0 1.0 5222 ARBHH 1452 3.0 1.0 2.0 2.0 2.0 0.0 1.0 0.0 1.0 5223 GSHH-2595 2.0 0.0 1.0 2.0 0.0 0.0 1.0 0.0 1.0 5224 Z C (Ankur 651) 2.0 1.0 1.0 2.0 1.0 0.0 1.0 0.0 1.0 5225 RAHH 909 3.0 0.0 2.0 2.0 1.0 0.0 1.0 0.0 1.0 5226 Local Check 3.0 1.0 0.0 2.0 1.0 0.0 1.0 0.0 1.0 5227 NHH 715 2.0 0.0 2.0 1.0 0.0 0.0 1.0 0.0 1.0 5228 DHH 1252 2.0 1.0 2.0 2.0 1.0 0.0 1.0 0.0 1.0 5229 GTHH 217 3.0 0.0 2.0 1.0 1.0 0.0 1.0 0.0 1.0 5230 SHH 902 2.0 0.0 2.0 2.0 0.0 0.0 1.0 0.0 1.0 SC-LRA 5166 (NAD) 3.0 2.0 1.0 SC-LRA 5166 Bharuch 2.0 1.0 0.0 SC-LRA 5166 (AKL) 2.0 1.0 1.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 68

Table 57: CZ 06 (b)

BB MLS GM Code Entry SRT AKL AKL AKL 5231 GSHV 171 1.0 2.0 1.0 1.0 5232 ANGC 1452 1.0 1.0 1.0 1.0 5233 JK 35 0.0 2.0 1.0 1.0 5234 Local Check (Coded & Closer spacing) 0.0 2.0 1.0 1.0 5235 ANGC 1451 1.0 2.0 1.0 1.0 5236 DSC-1352 1.0 1.0 1.0 1.0 5237 AKH 13-51 0.0 1.0 1.0 1.0 SC-LRA 5166 (AKL) 2.0 1.0 1.0 SC-LRA 5166 (SRT) 3.0

Table 58: CZ 24(b)

ALB BB MLS GM Code Entry JNG AKL JNG AKL AKL 5241 JLA 0603 2.0 1.0 0.0 1.0 1.0 5242 NDLA 3020 2.0 1.0 0.0 1.0 1.0 5243 PA 785 2.0 1.0 0.0 1.0 1.0 5244 Zonal Check (AKA 7) 2.0 1.0 0.0 1.0 1.0 5245 JLA-0614 2.0 1.0 0.0 1.0 1.0 5246 Local Check 2.0 1.0 0.0 1.0 1.0 5247 NDLA 3068 2.0 1.0 0.0 1.0 1.0 SC-LRA 5166 (JNG) 3.0 2.0 SC-LRA 5166 (AKL) 1.0 1.0 1.0

Table 59: CZ 25(b)

ALB BB MLB/MLS GM Code Entry JNG KDW JNG AKL KDW AKL KDW KDW AKL 5251 AKDH-96 2.0 2.0 0.0 1.0 2.0 1.0 2.0 3.0 1.0 5252 Local Check 2.0 2.0 0.0 1.0 2.0 1.0 2.0 3.0 1.0 5253 NACH 433 2.0 2.0 0.0 1.0 3.0 1.0 2.0 2.0 1.0 5254 PKV DH 1 (Zonal Check) 2.0 2.0 0.0 1.0 2.0 1.0 2.0 2.0 1.0 SC-LRA 5166 (JNG) 3.0 2.0 SC-AKA8 (AKL) 1.0 1.0 1.0 SC-JK4 (KDW) 2.0 3.0 3.0 2.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 69 SOUTH ZONAL TRIALS

Table 60: SZ Br.03a ALS grade RUST Root Rot% BB GM TSV% Code Entry CBE GUN GUN CBE CBE CBE GUN CBE 5501 ARBH 1401 1.0 3.0 2.0 0.0 0.0 0.0 3.0 4.0 5502 Zonal Check (Suraj) 1.0 3.0 3.0 0.0 0.0 0.0 2.0 4.0 5503 GSHV 177 1.0 4.0 3.0 5.0 1.0 0.0 4.0 2.0 5504 GJHV 497 1.0 3.0 3.0 0.0 0.0 0.0 3.0 3.0 5505 Local Check 1.0 3.0 2.0 0.0 0.0 0.0 3.0 4.0 5506 TSH 0533-1 1.0 4.0 3.0 0.0 0.0 0.0 3.0 4.0 5507 CCH 14-1 1.0 3.0 3.0 0.0 1.0 1.0 2.0 2.0 5508 Shakti Sultan (SSGR105) 1.0 3.0 3.0 0.0 0.0 1.0 4.0 3.0 SC-RCH BGII (CBE) 1.0 0.0 0.0 0.0 3.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0

Table 61: SZ Br.04a ALS grade RUST Root Rot% GM BB TSV% Code Entry CBE GUN GUN CBE GUN CBE CBE 5511 HS 292 1.0 3.0 4.0 5.0 2.0 0.0 0.0 5512 CCH 12-2 1.0 3.0 3.0 0.0 3.0 0.0 2.0 5513 Local Check 1.0 3.0 3.0 5.0 2.0 1.0 0.0 5514 CCH 13-2 1.0 3.0 3.0 0.0 1.0 0.0 2.0 5515 GJHV-516 1.0 3.0 3.0 0.0 3.0 0.0 0.0 5516 Zonal Check (Suraj) 1.0 4.0 3.0 0.0 2.0 0.0 0.0 5517 TSH 04/115 1.0 3.0 3.0 0.0 3.0 0.0 0.0 SC-RCH BGII (CBE) 1.0 0.0 0.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0

Table 62: SZ Br.05a ALS RUST GM TSV% Code Entry CBE GUN GUN GUN CBE 5521 RHH-1007 2.0 2.0 3.0 3.0 3.0 5522 Zonal Check (Bunny) 1.0 2.0 3.0 3.0 2.0 5523 LAHH 26 1.0 2.0 3.0 3.0 4.0 5524 RAHH 909 1.0 3.0 3.0 3.0 3.0 5525 SHH 818 1.0 2.0 3.0 2.0 3.0 5526 Local Check 1.0 2.0 2.0 2.0 3.0 5527 NCS 5657 2.0 3.0 4.0 3.0 2.0 5528 SHH 902 1.0 2.0 3.0 3.0 2.0 5529 LAHH 25 2.0 2.0 4.0 3.0 3.0 5530 BGDHH 821 2.0 2.0 4.0 3.0 4.0 5531 Ankur Suvarna 1.0 3.0 3.0 3.0 2.0 SC-RCH BGII (CBE) 1.0 4.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 70

Table 63: SZ Br.0 6a ALS BB RUST GM TSV% Code Entry CBE GUN CBE GUN GUN CBE 5541 DSC-1402 1.0 4.0 0.0 2.0 3.0 3.0 5542 DSC-1301 1.0 3.0 0.0 2.0 3.0 2.0 5543 RAHC 1012 2.0 4.0 0.0 2.0 4.0 4.0 5544 Local Check (Coded & Closer spacing) 1.0 3.0 1.0 2.0 2.0 7.0 5545 DSC-1302 1.0 3.0 0.0 3.0 3.0 3.0 5546 CNH 25 1.0 3.0 0.0 3.0 3.0 5.0 5547 ARBC-1301 1.0 3.0 0.0 3.0 3.0 4.0 5548 GTHV 13/32 1.0 3.0 1.0 3.0 3.0 3.0 SC-RCH BGII (CBE) 2.0 0.0 3.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0

Table 64: SZ Br –13(a) Root AB BB GM Rust TSV% Code Entry rot% DWD GUN CBE DWD DWD GUN DWD GUN CBE CBE 5551 DB 1402 ------5552 DB 1401 ------5553 CCB 29 4.0 - 1.0 4.0 2.0 - 3.0 - 0.0 3.0 5554 ZC (Suvin) 3.0 - 1.0 2.0 2.0 - 3.0 - 6.0 4.0 5555 ARBB 1401 2.0 2.0 1.0 2.0 2.0 0.0 3.0 2.0 0.0 2.0 5556 ARBB 1402 3.0 3.0 1.0 1.0 2.0 1.0 3.0 3.0 0.0 3.0 SC-RCH BGII (CBE) 1.0 0.0 4.0 SC-Jadoo BGII 3.0 4.0 4.0 (GUN) SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

Table 65: SZ Br – 14(a) AB BB GM Rust TSV% Code Entry DWD GUN CBE DWD DWD GUN DWD GUN CBE 5561 GSB 43 3.0 2.0 1.0 2.0 2.0 0.0 3.0 3.0 3.0 5562* DB-1301 ------5563 Z C (Suvin) 3.0 3.0 1.0 3.0 2.0 0.0 4.0 2.0 4.0 5564 GSB-44 3.0 2.0 1.0 2.0 2.0 0.0 3.0 2.0 3.0 5565 ARBD 27 3.0 1.0 1.0 2.0 1.0 0.0 4.0 2.0 3.0 5566 DB-1302 ------SC-RCH BGII (CBE) 1.0 3.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 71

Table 66: SZ Br – 15(a)

AB BB GM Rust TSV% Code Entry DWD GUN CBE DWD DWD GUN DWD GUN CBE 5571 RHB-1014 4.0 3.0 1.0 2.0 2.0 0.0 4.0 3.0 2.0 5572 Z C (DCH 32) 4.0 3.0 1.0 2.0 2.0 1.0 4.0 3.0 3.0 5573 RHB 1123 4.0 3.0 1.0 2.0 1.0 0.0 4.0 3.0 2.0 5574 ARBHB 1402 4.0 2.0 1.0 2.0 1.0 1.0 4.0 2.0 4.0 5575 DHB 1009 4.0 3.0 1.0 2.0 2.0 1.0 4.0 3.0 2.0 5576 Local Check 4.0 3.0 1.0 2.0 2.0 1.0 4.0 3.0 3.0 5577 RHB 1122 4.0 3.0 1.0 2.0 2.0 1.0 4.0 4.0 2.0 5578 DHB 1012 4.0 3.0 1.0 1.0 1.0 1.0 4.0 4.0 2.0 5579 DHB-1301 4.0 3.0 1.0 2.0 2.0 1.0 4.0 3.0 2.0 SC-RCH BGII (CBE) 1.0 3.0 SC-Jadoo BGII (GUN) 3.0 4.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

Table 67: SZ Br- 03 (b)

AB BB GM Rust Code Entry DWD DWD DWD DWD 5581 GBHV 183 3.0 2.0 2.0 4.0 5582 Local Check 4.0 2.0 2.0 4.0 5583 GTHV 13/17 4.0 2.0 2.0 3.0 5584 SCS 1207 3.0 2.0 2.0 4.0 5585 QC (Suraj) 4.0 1.0 2.0 4.0 5586 Zonal Check (Sahana) 4.0 2.0 2.0 4.0 5587 SCS 1061 3.0 1.0 2.0 4.0 5588 GBHV 188 3.0 2.0 2.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

Table 68: SZ Br –04(b)

AB BB GM Rust Code Entry DWD DWD DWD DWD 5591 SCS 1062 3.0 1.0 2.0 3.0 5592 QC (Suraj) 4.0 2.0 1.0 4.0 5593 IH 11 4.0 2.0 2.0 4.0 5594 Local Check 4.0 2.0 1.0 4.0 5595 BGDS 802 4.0 1.0 2.0 3.0 5596 Zonal Check (Sahana) 4.0 2.0 2.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 72

Table 69: SZ Br – 05(b) AB BB GM Rust Code Entry DWD DWD DWD DWD 5601 SHH 818 4.0 2.0 2.0 4.0 5602 ARBHH 1452 3.0 1.0 2.0 4.0 5603 NHH 715 4.0 2.0 2.0 4.0 5604 Local Check 4.0 2.0 1.0 4.0 5605 GTHH 217 4.0 2.0 2.0 4.0 5606 Zonal Check (Bunny) 4.0 2.0 2.0 4.0 5607 ARBHH-1352 4.0 1.0 2.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

Table 70: SZ Br- 06(b) AB BB GM Rust Code Entry DWD DWD DWD DWD 5611 GSHV 171 4.0 2.0 2.0 4.0 5612 ANGC 1451 4.0 2.0 2.0 4.0 5613 CNH 1111 3.0 2.0 2.0 4.0 5614 DSC-1351 4.0 2.0 2.0 4.0 5615 Local Check (Coded & Closer spacing) 4.0 2.0 2.0 4.0 5616 SCS 1206 4.0 2.0 2.0 4.0 5617 DSC-1451 4.0 2.0 2.0 4.0 5618 ARBC-1352 4.0 2.0 2.0 4.0 5619 ARBC-1452 3.0 2.0 2.0 4.0 5620 ANGC 1452 4.0 2.0 2.0 4.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

Table 71: SZ Br – 24(b) AB BB GM Rust Code Entry DWD DWD DWD DWD 5621* ARBa1401 - - - - 5622 Zonal Check (DLSa 17) 3.0 - 4.0 1.0 5623 CNA 449 3.0 - 4.0 1.0 5624 JLA-0603 3.0 - 4.0 1.0 5625 GAM-198 2.0 - 4.0 2.0 5626 Local Check 3.0 - 4.0 1.0 5627 DWDa 1402 3.0 - 4.0 2.0 5628 PA 740 2.0 - 4.0 1.0 5629 GAM 219 2.0 - 4.0 1.0 5630 AKA 2008-7 2.0 - 4.0 1.0 SC-Abhadita (DWD) 4.0 4.0 4.0 4.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 73

Path.2 (b) Confirmation and maintenance of disease resistant lines

Table 72: Screening of hirsutum entries in (confirmation) trial against bacterial blight Bb), Cotton leaf curl disease (CLCuD) and Fungal foliar leaf spots (FFLS) at Faridkot

Cotton leaf curl Fungal foliar leaf spot S. Bacterial blight (BB) Entry disease (CLCuD) (FFLS) No. PDI Reaction Mean (grade) Reaction Mean (grade) Reaction 1 F2164 47.5 S 2.0 MR 2.0 MR 2 H1471 40.3 S 2.0 MR 2.0 MR 3 SSGR105 47.1 S 2.0 MR 2.0 MR 4 RS 2013 58.8 HS 2.0 MR 2.0 MR 5 LH 2076 58.5 HS 2.0 MR 2.0 MR 6 RS 2727 61.5 HS 2.0 MR 2.0 MR 7 CSH 2931 40.0 S 2.0 MR 2.0 MR 8 SU-FLUM 27.8 MR 1.0 R 2.0 MR 9 TSH 0499 55.0 HS 2.0 MR 2.0 MR 10 LH 2108 51.3 HS 2.0 MR 2.0 MR 11 LH 2391 56.6 HS 2.0 MR 2.0 MR 12 Bihani-251 29.8 MR 1.0 R 1.0 R 13 LHH 144 55.2 HS 2.0 MR 2.0 MR 14 F 2501 59.2 HS 1.0 R 2.0 MR 15 RS 2711 54.0 HS 2.0 MR 2.0 MR 16 CSH 3232 59.7 HS 2.0 MR 2.0 MR 17 RS 2765 54.7 HS 1.0 R 2.0 MR 18 P 5629 73.4 HS 2.0 MR 2.0 MR 19 F 2228 64.1 HS 2.0 MR 2.0 MR 20 SCS1061 70.6 HS 2.0 MR 2.0 MR 21 MR 68 38.7 MS 1.0 R 2.0 MR 22 LH 2376 69.2 HS 2.0 MR 2.0 MR 23 F 2296 56.2 HS 2.0 MR 2.0 MR 24 F 2468 62.7 HS 2.0 MR 2.0 MR 25 RST9 100.0 HS 3.0 MS 3.0 MS

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 74

Table 73: Confirmation and maintenance of resistant breeding lines at Rahuri Entry Disease grade (ALB) Entry Disease grade (ALB) Br 03 (a) = 2 Br 13 (a) = 3 TCH 1777 2 MR ARBB 1302 1 R CCH 12-2 1 R ZC (Suvin) 1 R Br 04 (a) = 2 GSB 44 2 MR BGDS 1063 2 MR Br 14 (a) = 2 CCH 11-2 2 MR TCB 37 1 R Br 06 (a) = 1 ARBD 27 1 R GTHV 04/13 1 R LRA-5166 4 HS

Disease reaction of resistant breeding lines (National Trial) Entry Disease grade (ALB) Entry Disease grade (ALB) Br 02 (a) =5 Br 06 (a) = 5 GJHV 497 1 R AHGC 1402 2 MR CNH 5 1 R DSC 1401 1 R L 1008 1 R CSH 3113 3 S CNH 1118 2 MR H 1447 2 MR CPD 1401 2 MR RAHC 1011 1 R Br 12 (a) = 1 GSB 45 1 R LRA-5166 4 HS

Table73 (a): promising lines against bacterial blight at Rahuri Bacterial leaf blight Sr. No. Name of varieties Grade Reaction 2014-15 1 BGDS-1063 3 MS 2 NDLH 1943 1 R 3 BHV 180 2 MR 4 AKH 10-1 2 MR 5 SCH-1062 3 MS 2015-16 6 P-2151 1 R 7 ADB - 542 1 R 8 BS - 30 2 MR 9 BS - 79 2 MR 10 BGHV -180 2 MR 11 SCS - 793 2 MR 12 AKH- 8828 2 MR

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 75 Table 74: Confirmation and maintenance of resistant breeding lines at Akola Bacterial leaf blight Sr. No. Name of varieties Grade Reaction 2014-15 1 BGDS-1063 3 MS 2 NDLH 1943 1 R 3 BHV 180 2 MR 4 AKH 10-1 2 MR 5 SCH-1062 3 MS 2015-16 6 P-2151 1 R 7 ADB - 542 1 R 8 BS - 30 2 MR 9 BS - 79 2 MR 10 BGHV -180 2 MR 11 SCS - 793 2 MR 12 AKH- 8828 2 MR

Table 75: Confirmation and maintenance of resistant breeding lines at Surat Bacterial blight Alternaria leaf spot Sr. No. Name of Entry Field Artificial Field Artificial Reaction Reaction reaction reaction reaction reaction 1 GSHH 2729 0 0 DF 0 0 DF 2 GSHV 162 0 0 DF 0 0 DF

Table 76: Confirmation and maintenance of resistant breeding lines at Junagadh Sr. Alternaria leaf spot Bacterial leaf blight Grey mildew Variety / year of testing No. Grade Reaction Grade Reaction Grade Reaction 1 RHC 0717 3 MS 1 R - - 2 GSHV 159 3 MS 1 R - - 3 H 1454 4 S 1 R - - 4 H 1462 3 MS 1 R - - 5 GJHV 500 4 S 1 R - - 6 GJHV 516 3 MS 1 R - - 7 TCH 1777 3 MS 2 MR - - 8 GJHV 511 3 MS 1 R - - 9 GSHV 164 3 MS 1 R - - 10 CNH 1116 3 MS 1 R - - 11 GSHV 169 3 MS 1 R - - 12 KH 1301 3 MS 1 R - - 13 CNH 5 3 MS 1 R - - 14 H 1471 3 MS 1 R - - 15 RS 2765 3 MS 1 R - - 16 CSH 3232 3 MS 1 R - - 17 BS-26 3 MS 1 R - -

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 76

Table 77: Confirmation and maintenance of resistant breeding lines at Guntur

Disease Grade (0-4 Scale) Variety Selected for disease (Grade) Grey mildew Alternaria Leaf Spot ARBB 1401 Grey mildew (1) 1 - Alternaria leaf spot (2) - 2 DB 1402 Grey mildew (1) 1 - Alternaria leaf spot (2) - 2 CCB 36 Grey mildew (1) 1 - Alternaria leaf spot (2) - 2 ARBB 1402 Grey mildew (1) 1 - Alternaria leaf spot (2) - 2 DB 1302 Grey mildew (1) 1 - Alternaria leaf spot (2) - 2 Jadoo Grey mildew 4 - Alternaria leaf spot - 3 RCH 2 BG II Bacterial blight - - Rust - 4

Table 78: Confirmation and maintenance of resistant breeding lines at Dharwad

Foliar Diseases Grades Sl. No Genotypes ( 0 to 4 Scale ) AB BB GM Rust 1 DB-14 2 2 1 4 2 SVA-1118 1 - - 1 3 GSB-41 3 2 1 4 4 DB-40 2 2 2 4 5 DB-16 3 2 2 4 6 ZC-(Suvin) 3 4 3 4 7 SCS-1206 2 2 2 4 8 RHB-1005 3 2 2 4 9 DB-1302 3 2 2 3 10 GSHB-989 3 4 2 3 Jayadhar 4 - 4 4 Abhadita 4 4 4 4

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 77

Table 79: Confirmation and maintenance of resistant breeding lines at TNAU (CBE)

S. Alternaria Leaf Myrothecium leaf Root Grey Bacterial Leaf Entries No. spot spot rot Mildew Blight 1. 4501 + + + + - 2. 4502 + - - - + 3. 4503 + - - - - 4. 4504 + + - - - 5. 4505 + - - - - 6. 4506 + - - - - 7. 4507 + - - - - 8. 4509 + - - - - 9. 4511 + - - - - 10. 4512 + - + - - 11. 4513 + - - - - 12. 4514 + - - - + 13. 4515 + + - - - 14. 4516 + - + + - 15. 4532 + - + - - 16. 4535 + - - - - 17. 4538 + - - - - + Disease incidence - No disease incidence

Table 80: Path 2 (c): Monitoring of breakdown of resistance against CLCuD in cotton

CLCuD CLCuD CLCuD Name of Cumulative Cumulative (PDI) (PDI) (PDI) variety/hybrid mean reaction BHT SNG HSR HS6 79.6 70.1 71.7 73.8 HS RST9 84.9 68.4 62.8 72.0 HS F846 100.0 65.3 53.3 72.9 HS RCH134 BGII 58.8 78.1 60.6 65.8 HS H1236 64.1 58.3 62.8 61.7 HS LH2076 37.8 47.7 66.7 50.7 HS RS2013 69.2 45.1 70.0 61.4 HS RCH650 29.9 33.4 70.6 44.6 S

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 78

Table 81: Path 3a1: Efficacy of seed dressing chemicals on seed and soil borne diseases of Cotton (Pooled data: 2012-2014)

BB Root Seed mortality % AB PDI Yield (q/ha) C:B / *IBCR ratio Treatments PDI rot % DWD GUN CBE mean DWD GUN CBE mean DWD GUN CBE mean DWD CBE DWD CBE *GUN Thiram 75WS @2g/kg 25.8 12.8 14.4 17.7 32.3 7.1 11.7 17.1 12.5 7.8 6.5 8.9 14.6 12.0 1.64:1 01:1.5 4.9 Thiram 75WS @3g/kg 23.3 8.2 11.6 14.4 31.6 5.8 11.1 16.2 12.8 8.3 7.2 9.4 14 10.3 1.67:1 01:1.9 4.9 Thiram 75WS @4g/kg 23.8 4.6 10.5 13.0 32.3 5.5 10.5 16.1 13.0 9.1 7.3 9.8 13.9 9.0 1.69:1 01:2.0 4.9 Carboxin 75% WP @ 22.9 13.3 14.4 16.9 30.7 6.4 12.7 16.6 12.6 8.0 6.2 8.9 14.5 11.8 1.64:1 01:1.0 4.9 1g/kg Carboxin 75% WP @ 24.1 10.2 12.4 15.6 31.3 5.7 11.2 16.0 12.6 9.2 6.4 9.4 14.3 10.5 1.65:1 01:1.4 4.9 2g/kg Carboxin 75% WP @ 24.7 4.1 11.1 13.3 31 4.6 10.8 15.4 13.1 9.4 6.6 9.7 14.8 9.4 1.69:1 01:1.5 4.9 3g/kg Carboxin 37.5% WP + Thiram 37.5 % DS 25.4 7.9 13.1 15.5 30.3 5.6 11.8 15.9 13.2 8.3 7.04 9.5 15 10.5 1.71:1 01:1.7 4.8 2.5g/kg Carboxin 37.5% WP + Thiram 37.5 % DS 25.0 6.2 9.2 13.5 26 5.2 8.8 13.3 14.6 9.5 7.6 10.6 15.2 9.2 1.85:1 01:2.3 4.1 3.5g/kg Carboxin 37.5% WP + Thiram 37.5 % DS 20.2 4 6.5 10.2 25.5 4.1 8.5 12.7 14.8 9.6 7.9 10.7 14.7 8.2 1.87:1 01:2.6 4.8 4.5g/kg Untreated control 22.2 16.2 35.1 24.5 35.8 15.4 14.7 21.9 12.0 6.9 4.4 7.8 20.5 23.4 1.58:1 CD(0.05) 8.8 2.7 8.2 3.1 1.3 3.8 0.7 1.1 0.8 2.3 1.9 CV% 17.7 34.6 11.1 19.9 7.0 7.2 9.5

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 79

Table 82: Path 3(a3): Evaluation of TrichoCASH (Trichoderma harzianum) CICR-G 1% WP for cotton root diseases at Sirsa. Seedling mortality% T1- Control 29.3 T2- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(5g/kg seed) 28.1 T3- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(10g/kg seed) 27.3 T4-Standard chemical seed treatment –Thiram@3g/kg seed 27.0 T5-Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(5g/kg seed) + Thiram@3g/kg seed 25.7 T6- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(10g/kg seed) + Thiram@3g/kg seed 24.8 T7-Any locally available Trichoderma commercial formulation seed treatment (5g/Kg seed) 29.3 T8- Any locally available Trichoderma commercial formulation seed treatment (10g/Kg seed) 28.1 T9- Trichocash-Seed treatment(5g/kg seed) + Drenching at the base of the plant after one month with 200ml prepration @ 0.5% 27.2 T10- Trichocash-Seed treatment(10g/kg seed) + Drenching at the base of the plant after one month with 200ml prepration @ 1.0% 28.4 CD (0.05) N/A CV% 18.6

Root rot mortality% T1-Control 48.1 T2- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(5g/kg seed) 46.3 T3- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(10g/kg seed) 47.2 T4-Standard chemical seed treatment –Thiram@3kg seed 48.6 T5-Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(5g/kg seed) + Thiram@3kg seed 43.9 T6- Tricho CASH(Trichoderma harzianum) CICR-G 1% WP-Seed treatment(10g/kg seed) + Thiram@3kg seed 44.0 T7-Any locally available Trichoderma commercial formulation seed treatment (5g/Kg seed) 48.4 T8- Any locally available Trichoderma commercial formulation seed treatment (10g/Kg seed) 41.1 T9- Trichocash-Seed treatment(5g/kg seed) + Drenching at the base of the plant after one month with 200ml prepration @ 0.5% 48.3 T10- Trichocash-Seed treatment(10g/kg seed) + Drenching at the base of the plant after one month with 200ml prepration @ 1.0% 47.7 CD (0.05) NS CV% 16.0

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 80

Table 83: Effect of TrichoCASH on Fusarium wilt of cotton at Pune.

Wilt incidence (%) Disease control Sr. No. Name of fungicide/ Bio control Agent Dose to be used for seed dressing g/ Kg or Lit after 45 days (%) 1 Control (No ST) 0 100 0.0 2 TrichoCASH 5 83.6 16.3 3 TrichoCASH 10 79.2 20.7 4 Thiram 3 67.8 32.2 5 TrichoCASH)+Thiram 5+3 56.6 43.3 6 TrichoCASH)+Thiram 10+3 54.7 45.2 7 Phule Tricho 5 76.7 23.2 8 Phule Tricho 10 74.5 25.4 9 TrichoCASH+Drenching of TrichoCASH at 30DAS 5+5 72.3 27.6 10 TrichoCASH+ Drenching of TrichoCASH at 30DAS 10+10 70.5 29.4

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 81

Path 3 (c): Developing IDM modules for the management of cotton diseases (Akola, Junagarh and Dharwad)

Table 84: Developing IDM modules for the management of cotton diseases at Akola

*Plant *Root Germination Days to *Boll Yield Treatment height length PDI PDC (%) Flowering no. Q/ha (cm) (cm) ST: Trichoderma viride (TV-TNAU) @10g/kg seed; SA: of T.viride T 96.7 68.0 38.0 42 18.4 5.2(2.2) 37.0 10.2 1 (TV-TNAU) @2.5kg/ha; FS: with T.viride (TV-TNAU) @1% ST: Bacillus subtilis (BSC5-TNAU) @ 10g/kg seed; SA: of B. T2 subtilis (BSC5-TNAU) @2.5kg/ha; FS: with B.subtilis (BSC5- 97.1 66.0 37.0 42 18.3 5.2(2.2) 36.9 12.4 TNAU) @1% ST: Pseudomonas fluorescens(PF-TNA @10g/kg seed; SA: of P. T 97.8 71.1 38.3 38 18.4 4.8(2.1) 41.9 13.5 3 fluorescens (PF-TNAU) @2.5Kg/ha; FS: with P. fluorescens @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed; SA: of T4 P.fluorescens(PF-CICR) @ 2.5 Kg/ha in 250kg of FYM ; FS: with 94.0 65.2 41.2 45 15.2 5.3(2.3) 34.8 9.3 P.fluorescens(PF-CICR) @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed ; SA: of T.viride (TV-TNAU) @2.5Kg/ha; FS: with Propiconazole 0.1% and T 96.4 68.4 39.0 39 20.8 4.7(2.1) 42.2 13.0 5 COC (0.3%) +Streptocycline (0.01%) for BLB or Carbendazim 0.1% for GM on need basis. ST: Pseudomonas fluorescens (PF-CICR)@ 10g/kg seed; SA: of T.viride (TV-TNAU) @2.5Kg/ha in 250kg of FYM ; FS: with T 97.8 67.2 38.2 40 20.3 5.2(2.2) 36.4 8.6 6 Ergon@ 1ml/lit followed by Taqat @1.5g/lit for fungal diseases or COC (0.3%)+ Streptocycline(0.01%) for BLB T Control 92.7 65.1 37.1 38 17.5 8.2(2.8) 8.4 7 S.E ± 0.10 0.3

C.D at 5% 0.3 1.1

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 82

Table 85: Developing IDM modules for the management of cotton diseases at Junagarh

Seed Alternaria Bacterial leaf Mortality Treatment cotton leaf spot PDI blight PDI per cent Yield Q/ha ST: Trichoderma viride (TV-TNAU) @10g/kg seed; SA: of T.viride (TV-TNAU) T 3.12 #(9.7) 1.5(2.4) 3.2(10.4) 17.0 1 @2.5kg/ha; FS: with T.viride (TV-TNAU) @1% ST: Bacillus subtilis (BSC5-TNAU) @ 10g/kg seed; SA: of B. subtilis (BSC5-TNAU) T 3.2(10.5) 1.5(2.3) 3.2(10.6) 17.1 2 @2.5kg/ha; FS: with B.subtilis (BSC5-TNAU) @1% ST: Pseudomonas fluorescens(PF-TNA @10g/kg seed; SA: of P. fluorescens (PF- T 3.6(13.6) 1.4(2.1) 3.4(11.7) 16.5 3 TNAU) @2.5Kg/ha; FS: with P. fluorescens @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed; SA: of P.fluorescens(PF- T 3.9(15.2) 1.7(3.1) 3.3(11.4) 15.6 4 CICR) @ 2.5 Kg/ha in 250kg of FYM ; FS: with P.fluorescens(PF-CICR) @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed ; SA: of T.viride (TV-TNAU) T5 @2.5Kg/ha; FS: with Propiconazole 0.1% and COC (0.3%) +Streptocycline (0.01%) 3.0(9.1) 1.4(1.9) 3.0(9.2) 17.5 for BLB or Carbendazim 0.1% for GM on need basis. ST: Pseudomonas fluorescens (PF-CICR)@ 10g/kg seed; SA: of T.viride (TV-TNAU) T6 @2.5Kg/ha in 250kg of FYM ; FS: with Ergon@ 1ml/lit followed by Taqat @1.5g/lit 2.9(8.7) 1.3(1.8) 2.8(8.3) 18.2 for fungal diseases or COC (0.3%)+ Streptocycline(0.01%) for BLB

T7 Control 4.0 (16.3) 2.0 (3.9) 3.9 (15.3) 12.4 S.Em.± 0.2 0.01 0.1 109 C.D. at 5% 0.6 0.3 0.5 33.7 C. V. % 10.4 10.2 10.0 11.5

#Transformed square root values, Numerals in parenthesis are retransformed value

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 83

Table 85a: Statement showing the germination percentage and plant height in IDM Modules at Junagarh

Germination Plant Height Days taken for Treatment % (Mean) (cm) (Mean) First flowering ST: Trichoderma viride (TV-TNAU)@10g/kg seed; SA: of T. viride (TV-TNAU) @2.5kg/ha; T 97.6 85 59 1 FS: with T. viride (TV-TNAU) @1% ST: Bacillus subtilis (BSC5-TNAU) @ 10g/kg seed; SA: of B. subtilis (BSC5-TNAU) T 97.0 87 60 2 @2.5kg/ha; FS: with B .subtilis (BSC5-TNAU) @1% ST: Pseudomonas fluorescens (PF-TNAU) @10g/kg seed; SA: of P. fluorescens (PF-TNAU) T 95.6 85 57 3 @2.5Kg/ha; FS: with P. fluorescens @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed; SA: of P. fluorescens(PF-CICR) @ T4 2.5Kg/ha in 250kg of FYM ; FS: with P. fluorescens(PF-CICR) 95.6 85 57 @ 1% ST: Pseudomonas fluorescens (PF-CICR) @10g/kg seed SA: of T. viride (TV-TNAU) T5 @2.5Kg/ha; FS: with Propiconazole 0.1% and COC (0.3%) + Streptocycline (0.01%) for 96.0 87 58 BLB or Carbendazim 0.1% for GM on need basis. ST: Pseudomonas fluorescens (PF-CICR)@ 10g/kg seed; SA: of T. viride (TV-TNAU) T6 @2.5Kg/ha in 250kg of FYM ; FS: with Ergon @ 1ml/lit followed by Taqat @1.5g/lit for 99.3 91 57 fungal diseases or COC (0.3%)+ Streptocycline(0.01%) for BLB

T7 Control 93.0 83 62

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 84

Table 86: Developing IDM modules for the management of cotton diseases at Dharwad

Seedling AB PDI Rust PDI Yield Treatments mortality (Q/ha) 120 DAS** 120 DAS** (%) Trichoderma viride (TV-TNAU) @ 10g/kg Seed Soil application @ 2.5 kg/ha foliar T 26.1(30.7) 23.2(28.7) 28.7(32.3) 22.7 1 spray with T. viride @1% Bacillus Subtills (BSC5-TNAU) @ 10g/kg Seed Soil application @ 2.5 kg/ha foliar T 24.3(29.5) 24.1(29.3) 29.2(32.6) 23.6 2 spray with B. Subtills @1% Pseudomonas fluorescens (PF-TNAU) Soil application @ 2.5 kg/ha foliar spray with T 24.8(29.8) 18.9 (25.7) 29.8(33.1) 21.3 3 P. fluorescens @1% Seed Treatment Pseudomonas fluorescens (PF-CICR) @ 10g/kg of seed Soil T4 application Pseudomonas fluorescens (PF-CICR) @ @ 2.5 kg/ha in 250 kg of 22.8(29.8) 18.7(25.6) 26.5(30.9) 23.2 Compost or FYM foliar spray with P. fluorescens @ 1%(PF-CICR) Trichoderma viride (TV-TNAU) @ 10g/kg of seed Soil application of Trichoderma viride @ 2.5 kg/ha(TV-TNAU) foliar spray with Propiconazole 0.1% for foliar T 25.0(29.9) 20.7(27.0) 26.9(31.2) 20.6 5 diseases and COC 0.3% + Streptocycline 0.01% for BLB or Carbendazim 0.1% for grey mildew on need basis Pseudomonas fluorescens (PF-CICR) @ 10g/kg of seed Soil application of Trichoderma viride @ 2.5 kg/ha(TV-TNAU) in 250kg of Compost or FYM foliar T 21.0(27.2) 23.0(28.6) 27.8(31.8) 24.8 6 spray with Ergon @ 1ml/lit followed by Taqat @ 1.5g/lit for Fungal diseases or COC 0.3% + Streptocycline 0.01% for BLB

T7 Untreated control 22.20(28.0) 22.30(28.1) 29.5032.8) 24.3 SEm± 0.9 1.4 1.2 0.16 CD at 5% 2.9 4.4 3.8 0.50

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 85

Path3 (e): Table 87: Innovative interventions for the management of CLCuD Mean % incidence of CLCuD After 15 days of spray mean Treatments 1st 2nd 3rd 4th 5th PDI spray spray spray spray spray T1 Butter milk @ 5% 10.0 51.1 77.5 90.8 100.0 53.6 T2 Cow urine @ 6.6 % 6.7 61.0 84.7 96.2 100.0 52.3 T3 Neem oil @ 1% 11.7 57.1 86.0 95.9 100.0 53.3 T4 Mustard oil @ 3% 8.3 46.8 83.3 93.6 100.0 53.6 T5 Calcium [email protected]% 12.5 54.8 83.1 90.0 100.0 55.5 Cow urine + Calcium T6 10.0 49.9 82.5 94.1 100.0 53.3 nitrate T7 Cow urine + Butter milk 12.0 65.0 90.7 97.9 100.0 54.1 Butter milk + Calcium T8 9.2 59.0 90.5 97.3 100.0 55.9 nitrate T9 Lachesis 30@ 0.1 % 7.5 57.1 91.0 96.4 100.0 55.9 T10 Digitalis 30@ 0.1 % 9.2 58.8 95.4 96.9 100.0 58.4 T11 Apis mallifera 30@ 0.1 % 6.7 51.9 79.6 92.6 100.0 54.7 T12 Bryonia 30@ 0.1 % 11.1 54.5 80.8 94.0 100.0 53.9 T13 Natrum Mure 30@ 0.1 % 9.4 48.2 76.8 93.9 100.0 54.9 T14 Polo @ 0.1 % 6.9 48.8 75.8 95.5 100.0 52.0 T15 Control 9.2 59.2 88.7 97.7 100.0 66.2

Table 87 (a): Yield (q/ha) Treatments FDK BHT HSR SRS SNG Butter milk @ 5% 12.4 28.1 8 28.2 26.1 Cow urine @ 6.6 % 14.0 22.1 7.5 28.5 26.3 Neem oil @ 1% 12.8 22.5 6.4 27.3 27.4 Mustard oil @ 3% 13.3 22.3 7.2 26.8 25.2 Calcium [email protected]% 13.1 19.5 7.3 27.4 26.3 Cow urine + Calcium nitrate 12.3 26.9 6 26.8 28.8 Cow urine + Butter milk 9.6 21.4 7.9 26.5 27.7 Butter milk + Calcium nitrate 13.3 16.7 8.1 26.8 28.3 Lachesis 30@ 0.1 % 11.9 15.1 8 27.4 28.3 Digitalis 30@ 0.1 % 10.6 19.1 7 26.8 27.9 Apis mallifera 30@ 0.1 % 10.1 20.8 7 27.4 27.2 Bryonia 30@ 0.1 % 11.7 19.5 5.9 27.7 28.3 Natrum Mure 30@ 0.1 % 12.2 15.2 6.7 27.4 27.4 Polo @ 0.1 % 11.9 15.2 6.9 31.5 28.8 Control 9.1 11.2 6.8 27.1 22.5 CD(0.05) N/A N/A N/A N/A N/A CV% 19.9 16.4 10.6 11.8 13.9

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 86

Table 87(b): Innovative intervention for the management of CLCuD (Whitefly) – Sirsa

1st Spray 2nd Spray 3rd Spray 4th Spray 5th Spray Treatment *BS *TV 7 DAS TV BS TV 7 DAS TV BS TV 7 DAS TV BS TV 7 DAS TV BS TV 7 DAS TV Butter milk @ 5% 1.4 1.5 1.6 1.3 4.6 2.3 2.8 1.9 15.7 4.0 21.7 4.6 31.4 5.6 29.4 5.4 27.3 5.3 23.1 4.9 Cow urine @ 6.6 % 0.8 1.3 0.6 1.2 5.8 2.5 4.7 2.4 23.0 4.9 19.6 4.4 41.3 6.5 34.7 5.9 25.2 5.0 22.6 4.8 Neem oil @ 1% 1.3 1.5 1.0 1.4 4.7 2.3 3.9 2.1 22.5 4.8 17.3 4.2 29.4 5.5 25.4 5.1 21.2 4.7 18.5 4.4 Mustard oil @ 3% 3.4 2.0 1.3 1.5 5.1 2.4 2.9 1.9 17.7 4.3 17.1 4.2 30.7 5.6 27.9 5.3 21.5 4.7 20.2 4.6 Calcium [email protected]% 2.5 1.8 1.8 1.6 6.8 2.7 4.1 2.2 18.2 4.3 27.8 5.3 32.7 5.8 34.3 5.9 25.1 5.1 20.1 4.5 Cow urine + Calcium nitrate 1.5 1.6 1.2 1.5 6.3 2.6 5.0 2.4 25.3 5.1 20.5 4.6 39.9 6.4 34.5 5.9 24.6 5.0 20.4 4.6 Cow urine + Butter milk 1.9 1.7 2.1 1.7 5.4 2.5 5.0 2.4 21.6 4.7 28.1 5.3 37.2 6.1 37.4 6.1 20.9 4.6 21. 4.7 Butter milk + Calcium nitrate 0.5 1.2 2.3 1.8 8.3 3.0 5.4 2.5 22.9 4.8 34.0 5.9 31.7 5.7 33.7 5.8 25.7 5.1 19.5 4.5 Lachesis 30@ 0.1 % 1.6 1.6 1.8 1.6 4.9 2.4 6.3 2.7 26.3 5.2 30.8 5.6 37.1 6.1 38.4 6.2 26.6 5.2 20.5 4.6 Digitalis 30@ 0.1 % 0.6 1.2 2.1 1.7 6.2 2.6 4.9 2.4 25.3 5.1 33.0 5.8 38.6 6.2 37.9 6.2 22.9 4.8 20.5 4.6 Apis mallifera 30@ 0.1 % 0.5 1.2 1.6 1.6 7.1 2.8 5.6 2.5 23.2 4.9 25.5 5.1 45.9 6.8 41.4 6.5 27.6 5.3 19.1 4.4 Bryonia 30@ 0.1 % 0.7 1.3 2.2 1.7 8.6 3.1 5.6 2.5 20.6 4.6 27.2 5.2 35.8 6.0 36.2 6.1 28.7 5.4 20.2 4.6 Natrum Mure 30@ 0.1 % 0.9 1.3 2. 1.7 7.6 2.9 6.1 2.6 22.4 4.8 28.9 5.4 39.1 6.3 34.2 5.9 24.2 5.0 20.4 4.6 Polo @ 0.1 % 3.3 2.0 2.1 1.7 6.4 2.7 4.6 2.3 26.8 5.2 20.0 4.5 44.0 6.7 27.8 5.3 26.9 5.2 19.8 4.5 Control 1.1 1.4 2.0 1.7 8.0 3.0 5.4 2.5 21.9 4.7 24.6 4.9 43.1 6.6 38.9 6.3 24.9 5.0 19.5 4.5 C.D. 0.4 0.1 N/A N/A N/A N/A N/A 0.73 N/A N/A SE(m) 0.1 0.0 0.2 0.2 0.3 0.5 0.3 0.2 0.4 0.1 SE(d) 0.1 0.1 0.3 0.2 0.4 0.7 0.5 0.3 0.5 0.2 C.V. 10.7 3.7 11.1 10.2 8.4 13.4 7.9 5.7 9.7 4.3

* - BS Before Spray *TV- Square root transformation

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 87

Table 87 (c): Innovative intervention for the management of CLCuD (Whitefly) - Hisar

Treatment Pre Spray 7 DAS Pre Spray 7 DAS Pre Spray 7 DAS Pre Spray 7 DAS Pre Spray 7 DAS Butter milk @ 5% 19.3 24.1 45.9 33.3 74.9 65.7 75.1 77.7 95.0 209.6 Cow urine @ 6.6 % 18.4 24.9 51.9 33.5 81.1 74.5 80.1 81.6 96.9 241.1 Neem oil @ 1% 20.1 23.7 47.5 32.2 86.3 79.8 86.1 74.6 96.5 233.2 Mustard oil @ 3% 19.0 22.5 42.2 30.7 66.1 61.8 79.3 79.1 91.3 248.7 Calcium [email protected]% 21.1 24.7 43.6 36.9 75.1 68.9 82.3 77.5 96.0 227.7 Cow urine + Calcium nitrate 21.6 23.4 45.3 31.7 72.6 65.3 72.5 67.6 93.9 230.6 Cow urine + Butter milk 21.3 21.5 47.1 35.7 75.1 73.6 71.9 67.7 103.3 266.3 Butter milk + Calcium nitrate 24.7 22.9 50.1 30.0 77.3 70.8 74.5 70.6 96.3 216.1 Lachesis 30@ 0.1 % 21.4 24.9 48.5 30.5 81.3 78.1 84.0 78.6 103.8 251.9 Digitalis 30@ 0.1 % 23.9 23.7 46.2 30.4 81.2 75.0 79.2 74.8 98.1 239.7 Apis mallifera 30@ 0.1 % 22.1 19.1 52.3 30.7 91.7 83.8 88.8 80.3 97.8 217.3 Bryonia 30@ 0.1 % 20.9 21.7 45.8 30.3 86.7 76.9 76.0 70.3 89.0 199.5 Natrum Mure 30@ 0.1 % 21.9 21.2 48.7 34.7 81.8 71.3 68.3 68.7 83.2 236.5 Polo @ 0.1 % 23.7 23.6 49.1 30.8 96.4 85.7 76.3 69.9 92.1 226.3 Control 25.4 23.3 47.6 29.9 84.9 78.3 83.1 76.7 89.8 242.1 C.D. 4.0 NS NS NS NS NS NS NS 8.8 NS C.V. 11.0 9.2 9.0 12.0 12.8 10.8 9.4 8.0 5.5 16.2

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 88

Table 87(d): Innovative intervention for the management of CLCuD (Whitefly) - Sriganganagar

1st spray 2nd spray 3rd spray 4th spray 5th spray Treatments B.S. 7 DAS B.S. 7 DAS B.S. 7 DAS B.S. 7 DAS B.S. 7 DAS T1 Butter milk @ 5% 8.4(2.8*) 5.2(2.2*) 81.7(9.0*) 48.9(6.9*) 42.6(6.5*) 24.2(4.9*) 64.8(8.0*) 41.2(6.4*) 15.7(3.9*) 8.3(2.8*) T2 Cow urine @ 6.6 % 8.1 (2.8) 4.40(2.09) 86.6 (9.3) 46.9 (6.8) 45.1 (6.7) 23.4 (4.8) 62.2 (7.8) 35.3 (5.9) 14.0 (3.7) 6.7 (2.5) T3 Neem oil @ 1% 8.0 (2.8) 3.9 (1.9) 80.4 (8.9) 37.90(6.1) 47.9 (6.9) 21.1 (4.5) 61.3 (7.8) 30.4 (5.5) 11.1(3.3) 4.8(2.1) T4 Mustard oil @ 3% 8.3 (2.8) 4.5(2.1) 85.5(9.2) 46.0(6.7) 39.7(6.3) 20.5(4.5) 63.1(7.9) 36.5(6.0) 14.5(3.8) 7.2(2.6) Calcium T5 8.5 (2.9) 4.6(2.1) 81.2(9.0) 44.6(6.6) 41.4(6.4) 22.1(4.7) 66.4(8.1) 35.6(5.9) 13.7(3.7) 6.4(2.5) [email protected]% Cow urine + Calcium T6 9.1(3.0) 4.70(2.17) 82.9(9.1) 41.2(6.4) 41.7(6.4) 19.8(4.4) 60.9(7.8) 31.2(5.5) 12.6(3.5) 5.6(2.3) nitrate Cow urine + Butter T7 9.4 (3.0) 5.10(2.26) 87.8(9.3) 47.1(6.8) 42.8(6.5) 21.7(4.6) 64.1(8.0) 35.8(5.9) 14.1(3.7) 6.7(2.5) milk Butter milk + T8 9.3 (3.0) 4.90(2.21) 85.0(9.2) 44.2(6.6) 43.0(6.5) 21.4(4.6) 65.0(8.0) 35.4(5.9) 14.7(3.8) 6.9(2.6) Calcium nitrate T9 Lachesis 30@ 0.1 % 9.7(3.1) 5.1(2.2) 86.3(9.2) 44.4(6.6) 41.4(6.4) 20.1(4.4) 61.6(7.8) 32.6(5.7) 16.60(4.0) 7.7(2.7) T10 Digitalis 30@ 0.1 % 9.3(3.0) 4.9(2.2) 90.6(9.5) 46.9(6.8) 43.3(6.5) 21.5(4.6) 66.2(8.1) 36.5(6.0) 16.5(4.0) 7.9(2.8) Apis mallifera 30@ T11 8.5(2.9) 5.1(2.2) 80.9(8.9) 48.1(6.9) 44.9(6.7) 26.6(5.1) 62.5(7.9) 41.1(6.4) 17.0(4.1) 9.3(3.0) 0.1 % T12 Bryonia 30@ 0.1 % 8.9(2.9) 4.8(2.1) 83.8(9.1) 44.5(6.6) 44.0(6.6) 22.2(4.7) 62.4(7.9) 34.6(5.8) 18.6(4.3) 8.9(2.9) Natrum Mure 30@ T13 8.8(2.9) 4.9(2.2) 91.1(9.5) 48.9(6.9) 42.6(6.5) 22.9(4.7) 62.5(7.8) 35.9(5.9) 18.2(4.2) 9.4(3.0) 0.1 % T14 Polo @ 0.1 % 9.8(3.1) 5.1(2.2) 89.7(9.46) 45.8(6.7) 49.1(6.9) 23.1(4.8) 61.7(7.8) 32.6(5.7) 18.8(4.3) 8.7(2.9) T15 Control 9.8(3.1) 8.7(2.9) 90.4(9.5) 75.5(8.6) 54.8(7.4) 46.7(6.8) 73.6(8.5) 67.2 (8.2) 28.0(5.2) 22.7(4.7) CV % 10 11.6 8.9 7.4 11.2 10.2 8.7 8.2 12.2 9.7 S.Em ± 0.7 0.4 6.2 2.8 4.2 2 4.5 2.5 1.6 0.6 CD at 5 % 2.2 1.4 19 8.7 12.9 6 13.8 7.6 4.9 2 B.S – Before Spray, DAS- Days after Spray, * - Square Root values

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 89

Path 4e Exp1: Estimation of crop losses due to CLCuD in Bt hybrids

Table 88: Path 4e (Exp 1): Seed cotton yield (%) reduction due to CLCuD in different Bt hybrids in North Zone 2015-16

Name of hybrids Hisar FDK SGN Mean Bioseed 6588 BGII 18.1 37.6 19.6 25.1 RCH650 BGII 20.0 28.6 17.3 22.0 Ankur Jai 3028 BGII 26.0 30.3 31.7 29.4 MRC 7017 BGII 10.3 31.7 34.8 25.6

Table 88 (a): Seed cotton yield (%) reduction disease grade wise in different Bt hybrids in North Zone

Bioseed 6588 BGII RCH650 BGII Ankur Jai 3028 BGII MRC 7017 BGII Grades Hisar FDK Hisar FDK Hisar FDK Hisar FDK 2 - 5.1 - 6.3 - 12.0 - 12.9 3 - 10.6 - 10.7 - 16.7 - 19.9 4 34.1 34.6 33.5 24.8 39.7 23.2 28.3 26.3 5 44.4 56.7 41.6 33.8 51.2 35.1 41.5 33.0 6 72.5 81.3 56.7 67.5 68.7 64.5 55.9 66.3 * Calculated based on lowest grades of plants available in each hybrid.

Table 89: Path 4 (e) Exp 2: Study on the distribution pattern of CLCuD on local popular Bt hybrids at farmers field in Punjab, Rajasthan and Haryana

Punjab Rajasthan Haryana Districts Mean PDI Districts Mean PDI Districts Mean PDI Faridkot 44.9 Sriganganagar 14.8 Sirsa 34.4 Fazilka 45.3 Fatehabad 25.1 Muktsar 35.3 Hisar 30.7 Bathinda 41.2 Jind 28.5 Mansa 34.0 Bhiwani 8.6 Rohtak 14.6 Sonipat 10.3

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16) F - 90

Table 90: Exp 3 – Incidence (%) of CLCuD in different Bt hybrids during at Sirsa

Name of hybrid 50DAS* 65DAS 80DAS 95DAS 110DAS 125DAS 140DAS YUVA-841-2BGII 0.0 0.2 0.4 1.2 1.6 2.9 4.2 BUNTY 2113-2 0.0 1.7 1.03 6.02 8.09 9.1 14.1 ANKUR JAI Bt 2.1 15.5 16.9 22.0 26.6 42.1 48.7 Ankur 3028 7.0 3.9 22.7 30.4 43.5 56.6 57.9

Table 90(a): CLCuD Disease Index (PDI) & yield in different Bt hybrids

Name of hybrid 80DAS* 110DAS 140DAS Yield(q/ha) YUVA-841-2 BGII 0.01 1.3 1.4 6.9 BUNTY 2113-2 0.6 4.2 4.8 9.2 ANKUR JAI Bt 5.4 11.2 22.7 8.3 Ankur 3028 9.0 24.4 30.9 9.2

ICAR - All India Coordinated Research Project on Cotton – Annual Report (2015 – 16)