Chemicals in Molars and Perinatal Exposure

Chemicals in molars and perinatal exposure

Acetaminophen, pesticide and diethylhexyl phthalate metabolites, anandamide, and fatty acids in deciduous molars: potential biomarkers of perinatal exposure

David E. Camann, Stephen T. Schultz, Alice Y. Yau, Lynne P. Heilbrun, Michelle M. Zuniga, Raymond F. Palmer, Claudia S. Miller

Supplementary Information

Characteristics of 21 Donors of 25 Molars

We obtained and assayed 25 molars from 21 molar donors at a local dental clinic to better assess the detection frequency, concentration distribution, and within-subject variability of selected chemicals in deciduous molars in the local general population. Demographic characteristics of the 21 molar donors are presented in Table S1. The subjects are predominantly Hispanic, and all except one were born between 1998 and 2005. Two donated molars were selected for assay from four of the subjects to explore the within-subject variability in the concentrations of detected chemicals measured in the molar pairs. We looked at whether nearly simultaneously formed molars (ie, both first molars or both second molars assayed) showed less measurement variability than when the formation windows were more separated (one first molar and one second molar assayed). Pertinent characteristics of the molars were noted before preparation for assay and are described in Table S2: whether extracted or exfoliated and whether the molar had visible cavities, fillings, and/or attached roots. Fewer of the molars had been shed before donation than were extracted at the dental clinic. Most of the molars contained cavities and few had been filled.

Concentrations of Individual LC/MS/MS Analytes and Fatty Acids in the 25 Molars

The individual measured concentrations of the 13 analytes and recovery of the spiked

acetaminophen-d4 surrogate determined by LC/MS/MS in the 25 molars obtained at the local dental clinic are presented in Table S3 in an excel file. Recoveries of each spiked and equilibrated target

1 Chemicals in molars and perinatal exposure chemical from aliquots of three of these powdered molars are also given in Table S3. The individual measured concentrations of the five omega 3 and four omega 6 fatty acids and recovery of the spiked triheneicosanoin surrogate determined by GC/MS/SIM in the 25 molars are presented in Table S4 in an excel file. Recoveries of each spiked and equilibrated target fatty acid from aliquots of two of these powdered molars are also given in Table S4.

2 Chemicals in molars and perinatal exposure

Table S1. Demographic characteristics of the 21 local molar donors Annual Donor ID Birth year Gender Ethnicity family income 0005 1998 M Hispanic 50-75K 0009 2002 F Hispanic < 15K 0076 2001 F Hispanic 35-50K 0080 2000 F Hispanic < 15K 0086 1985 M Hispanic 75-100K 0094 2000 F Hispanic 35-50K 0104 2005 M Hispanic 35-50K 0180 2001 M Hispanic < 15K 0188 2002 M Hispanic < 15K 0195 2004 M Hispanic NR 0232 2005 M Caucasian 35-50K 0428 2004 F Hispanic 15-25K 0535 2005 M Hispanic 35-50K 0549 2001 F Hispanic 25-35K 0550 2002 F Hispanic < 15K 0556 2003 F Hispanic NR 0562 2000 M Hispanic < 15K 0569 1998 F Hispanic NR 0572 2001 M Hispanic < 15K 0587 2004 M Hispanic NR 0590 2001 M Hispanic < 15K NR = Not reported

3 Chemicals in molars and perinatal exposure

Table S2. Characteristics of 25 assayed molars donated at dental clinic

Subject- Root Molar Shed or Date attached Any Number and ID pulled donated to tooth? cavities? type of fillings Comment 0005-L Shed No No 1 - amalgam 0009-J Pulled 7-27-2011 Yes 2 0 0076-I Pulled Yes Yes 0 0080-Z Shed No No 0 ½ of molar: unidentifiable 0086-S Shed No No 0 0094-T Pulled Yes No 0 0104-A Pulled Yes No 0 0180-A Shed Partial No 1 – amalgam 0188-T Shed 8-3-2011 No Yes 1 - amalgam 0195-T Pulled Yes Yes 1 – amalgam 0232-B Pulled Yes Yes 0 badly decayed 0428-S Shed No No 0 0535-K Pulled 8-31-2011 Yes 2 1 - temporary 0549-J Shed 8-11-2011 No 4 0 0550-K Shed 8-30-2011 No 1 - large 0 0550-L Shed 8-30-2011 No 1 0 0556-T Pulled Yes 1 0 0562-A Shed 9-13-2011 No 0 0 0562-J Shed 9-13-2011 No 0 0 0569-A Pulled Yes 1 0 0569-B Pulled Yes 1 0 0572-S Pulled 8-16-2011 Yes Yes 0 0587-I Pulled Yes 1 - large 0 0587-K Pulled Yes 2 0 0590-B Pulled 8-23-2011 Yes 1 0