Name: Nathalie Scholler (Née Buonavista), M.D., Ph.D

CURRICULUM VITAE

Name: Nathalie Scholler (née Buonavista), M.D., Ph.D.

Present Position: Tenure-track Assistant Professor, Department of Obstetrics and Gynecology. Office: University of Pennsylvania School of Medicine. Center for Research on Reproduction and Women's Health Biomedical Research Building II/III, Room 1309 421 Curie Blvd., Philadelphia, PA, 19104-6160 Phone: (215) 898 0164 – Fax: (215) 573 5129 – email: [email protected] http://www.med.upenn.edu/crrwh/faculty/Scholler/Scholler.html http://www.med.upenn.edu/schollerlab/

Place of Birth: Marseille, France Education: 1995 PhD, Immunology University of Science of Aix-Marseille II, France 1990 Diplôme d’études approfondies (equiv. MS) University of Science of Aix-Marseille II, France. In Immunology 1988 MD, General Medicine School of Medicine, Marseille, France.

Training: 1995.1997 Post-Doctoral FellowshipAutoimmunity Department, Bristol Myers Squibb Pharmaceutical Institute, Seattle, WA 1991.1995.1.1 PhD Thesis Centre d’Immunologie de Marseille-Luminy, University of Aix-Marseille II, France.

1986-1988 Internship, General Medicine University of Aix-Marseille Affiliated Hospitals, Marseille, France. Additional Training: 1993 Human genome analysis: from YAC to gene The 11th Welcome Summer School. United Medical and Dental Schools of Guy’s and St Thomas’s Hospitals, London, UK 2004 Engineering of Recombinant Antibodies Cambridge Healthtech Institute, Needham, MA

Faculty Appointments and Professional Positions: Nov. 2007-present Tenure-track Assistant Professor Department of Obstetrics and Gynecology, University of Pennsylvania, Philadelphia, PA 2003-2007 Senior Staff Scientist & Laboratory Director Translational and Outcomes Research laboratory, Molecular Diagnostics Program, Fred Hutchinson Cancer Research Center, Seattle, WA. 2001-2003 Senior Research Scientist Department of Biochemistry, Seattle Genetics, Inc. Bothell, WA. 1997.2001 Staff Research Scientist Department of Tumor Immunology, Pacific Northwest Research Institute, Seattle, WA. 1990-1995 Sports Medicine Physician French National Federation of Judo, Marseille, Fr. 1989-1990 Physician Scientist French National Institute of Health and Medical Research (INSERM), France and Brazil. 1988-1989 Resident, Internal Medicine St Joseph Hospital, Marseille, France.

Academic and Institutional committees 2010-present Penn Institute for Immunology (Penn IFI), UPenn Member 2010-present Bioengineering Graduate Studies, Bioengineering Graduate Group, UPenn Member 2009-present Biomedical Graduate Studies, Immunology Graduate Group, UPenn Member 2008-present FOCUS Advisory Committee, UPenn Member 2008-present Immunobiology program, Tumor Immunology Interest Group Full Research Member Abramson Cancer Center, UPenn 2007-present Penn Ovarian Cancer Research Center (Penn OCRC), UPenn Member Memberships: 2010-present American Association for Cancer Research (AACR) 2010-2011 New York Academy of Sciences (NYAS) 2008-present Consortium for Functional Glycomics (CFG) 2008-present Institute for Translational Medicine and Therapeutics (ITMAT) 2001-present American Association of Immunologists (AAI) 2001-present American Association for the Advancement of Science (AAAS) 2001-present American Society of Gene and Cell Therapy (ASGCT) Scholarships, Fellowships and Awards: 2011 Junior Faculty Travel Award American Association of Immunologists (AAI 2011) San Francisco, CA. 2003-2004 Career Development Award Marsha Rivkin Center for Ovarian Cancer Research (MRCOCR), Seattle, WA. 2001-2003 Marsha Rivkin Center Scholar Award MRCOCR, Seattle, WA 1995 Private fellowship La Fondation pour la Recherche Médicale, France. 1991.1994 National scholarship French Ministry of Research & Technology, France. 1990 Private fellowship La Fondation pour la Recherche Médicale, France. 1980.1986 Regional scholarship Province des Bouches-du-Rhône, France. Organizing roles 2011 Host Break-out discussion at the 13th Annual Phage and Yeast Display of Antibodies & Proteins conference, Protein Engineering Summit (PEGS) - May 9-10 - Boston, MA. 2010 Moderator Center for Research on Reproduction and Women’s Health Annual Research Retreat and the Cuozzo lecture- May 19- University of Pennsylvania, PA. 2010 Block Chair 97th AAI Annual meeting, Tumor Immune Response session - May 7-10 - Baltimore, MD 2009 Block Chair 96th AAI Annual meeting, Tumor Immune Escape - May 8-12 - Seattle, WA 2008 Block Chair 2nd International Conference on Ovarian Cancer: State of the Art and Future Directions - June 26 - July 1- Rhodes, Greece Scientific Review, Consulting and Advisory roles: 2012 Ad hoc reviewer for the Cancer Drug Discovery committee of the American Cancer Society 2011 Scientific reviewer for DOD Study section: Ovarian Cancer Research Program, Detection- Diagnosis and Clinical and Experimental Therapeutic, DD-CET 2011 Scientific expert for Wolf, Greenfield & Sacks, P.C., Boston, MA. 2011 Scientific reviewer for Kentucky Science & Engineering Foundation. 2010 Consultant for Reckner Healthcare. 2010 Advisory Board Member at the Editorial Board of Recent Patents on Biomarkers. Since 2010 Advisory Board member for Cancer Targets and Therapeutics Annual Conferences. 2010 Scientific reviewer for the Health Research Council (HRC), New Zealand. 2010 Scientific reviewer for DOD Study section: Ovarian Cancer Clinical and Experimental Therapeutics. 2009-2011 Advisory Board tenured Member in the Editorial board of Journal of Proteome Research. 2009 Scientific reviewer for NIH Challenge grants in Health and Science Research (RC1). 2009 Scientific reviewer for The Health Research Board, Ireland. 2008 Scientific reviewer for Maryland Industrial Partnerships (MIPS). 2 2008 Scientific expert, Ernst & Young, Life and Health Science Advisory, Paris, France 2008 Scientific reviewer for DOD Study section: Ovarian Cancer Research Program. 2008 Consultant for Ernst & Young Life and Health Science Advisory, Paris, France. Editorial Positions 2011-2014 Associate Editor BMC cancer 2011-present Peer-Reviewer Disease markers. 2011-present Peer-Reviewer OMICS: A Journal of Integrative Biology 2011-present Peer-Reviewer Nature Communications. 2011-present Peer-Reviewer Biomarkers in Medicine 2011-present Peer-Reviewer Molecular Pharmaceutics. 2011-present Peer-ReviewerJournal of Controlled Release. 2010-present Peer-ReviewerCancer Immunology Immunotherapy. 2010-present Associate Editorial Board member Journal of Translational Research 2010-present Peer-Reviewer Journal of Proteomics 2009-present Peer-Reviewer Gynecologic Oncology 2009-present Peer-Reviewer PlosONE 2009-present Peer-Reviewer Clinical Science 2009-present Peer-Reviewer Cancer Epidemiology 2009-present Author-in-Chief Encyclopedia of Cancer, 2nd edition 2006-present Peer-Reviewer Cancer Letters 2008-present Peer-Reviewer Cancer Biology & Therapy 2008-present Peer-Reviewer Gynecologic and Obstetric Investigation 2008-present Peer-Reviewer Molecular and Cellular Proteomics Presentations 2011 Invited speaker “Novel mechanisms of polarization of tumor-infiltrating macrophages”. Complex Carbohydrate Research Center, University of Athens. December 5, Athens, GA. 2011 Invited speaker “Role of tumor-released glycoproteins during macrophage polarization”. Consortium for Functional Glycomics (CFG) breakout Session. November 9, Seattle, WA. 2011 Poster presentation “Development of T-bodies expressing CIR directed against Endosialin/TEM1 for immune- gene therapy against breast cancer”. Nathalie Scholler, Stephen P. Santoro, Shree Joshi, Aizhi Zhao, and George Coukos, Era of Hope 2011, August 2-5, Orlando, FL. 2011 Invited speaker “Paired yeast-display/secretory vectors for rapid isolation of site-specific biotinylated affinity reagents”, 13th Annual Phage and Yeast Display of Antibodies & Proteins conference, Protein Engineering Summit (PEGS) - May 9-10 - Boston, MA. 2011 Oral presentation “Tumor released mesothelin contributes to the polarization of tumor-infiltrating macrophages”, 14th Annual Meeting of the Translational Research Cancer Centers Consortium conference, Seven Springs Mountain Resort - Feb 24-26 - Seven Springs, PA 2011 Invited speaker “GPI anchors: much more than an alternative strategy for cell surface presentation” GTCbio’s 3rd Oncology Biomarker Conference, Jan 27-28, San Diego, CA. 2011 Poster presentation “Tumor-infiltrating B-cells express toll-like receptors and exert proangiogenic activity in ovarian cancer”. S. Nunez-Cruz, W. Quinn, D. Connolly, P. Gimotty, and N. Scholler. EMBO 2011 meeting. Immunology & Metabolism- Centre d’Immunologie de Marseille- Luminy, January 13-14, Marseille, France. 2010 Invited speaker “Role of B cells in cancer development”. 23rd Annual Immunology Retreat. November 5-7. Lancaster, PA 2010 Invited speaker “Novel mechanism for M2 polarization of tumor-infiltrating macrophages via GPI-anchored proteins”. Marsha Rivkin Center Ovarian Cancer Research Symposium, session: On the Horizon: Developing Therapeutics. Oct 28-29, Seattle, WA. 2010 Invited speaker “Tumor-infiltrating lymphocytes B (TIL-B) angiogenic properties contribute to tumor growth in presence of complement”. Department of Immunopathology, NIAID/NIH. June 3, Rockville, MD. 2010 Invited speaker “Study of the role of tumor-infiltrating B-cells in a transgenic mouse model of ovarian cancer”. Tumor Immunology Interest Group. May 20, University of Pennsylvania, PA.

Presentations (continue) 2010 Poster presentation "Mannose receptor binding triggers M2-macrophage polarization" Denarda Dangaj, Selene Nunez-Cruz, Aizhi Zhao and Nathalie Scholler. Canary Foundation Early Detection Symposium, May 25-27, Stanford, CA 2009 Invited speaker “Alkali-resistant, site-specific biotinylated, high affinity reagent for IgG capture”. Bio-Rad laboratories, Dec. 9, Hercules, CA. 2009 Invited speaker “Tumor-infiltrating B lymphocytes in ovarian cancer”. FCCC-UPENN Quarterly Ovarian Cancer Research meeting. Fox Chase Cancer Research Center, Dec. 3, Philadelphia PA. 2009 Invited speaker “Study of the role of CA125 and adipocytes in the alternative complement pathway during ovarian cancer physiopathology” and “Blocking of CA125/Mesothelin-dependent peritoneal metastasis with antibodies and biobodies”. Fred Hutchinson Cancer Research Center, Scientific Program Committee Meeting. Nov 16, Seattle WA. 2009 Poster presentation "Blocking tumor/macrophages cross-talk with anti-mannose receptor recombinant antibodies to trigger tumor rejection" Denarda Dangaj, Aizhi Zhao, Raphael Sandaltzopoulos, George Coukos and Nathalie Scholler. 2009 NCI Translational Science Meeting (TSM 2), session: Therapeutic Antibodies and Combination Therapies, November 5-7, Vienna, VA. 2009 Poster presentation “Characterization of Tumor Infiltrating-B cells (TIL-B) in a transgenic mouse model of serous ovarian cancer.” Nunez-Cruz, S., Guerra, M., Queen, W., Scott P., Connolly, D., Cancro, M.P., Gimotty, P., and Scholler, N. Frontiers in Basic Immunology, NIH, October 1-2, Bethesda, MD. 2009 Invited speaker “Rapid identification of high affinity reagents using a yeast-expressed recombinant antibody platform”, Dana Farber/Harvard Medical School, June 19, Boston, MA. 2009 Invited speaker “Systèmes d’expression d’anticorps recombinants dans la levure: Application en Immunociblage”. INSERM -U 928- TAGC & UMR 928 Université de la Méditerranée, June 10, Marseille-Luminy, France. 2009 Invited speaker “Scouting glycoproteins in ovarian cancer”. CRRWH annual retreat, University of Pennsylvania, May 27, Philadelphia, PA. 2009 Invited speaker “Yeast-expressed recombinant antibody platform for rapid identification of high affinity reagents”. Institute of System Biology, May 11, Seattle, WA 2009 Invited speaker “Role of tumor-released GPI-anchor proteins in macrophage polarization”. Centocor, University of Pennsylvania, March 16, Philadelphia, PA 2009 Invited speaker “Biobody technology.” Drexel Institute for Biotechnology and Virology Research at Pennsylvania Biotechnology Center of Bucks County, March 11, Doylestown, PA. 2009 Invited speaker “Yeast-expressed recombinant antibody (scFv) for discovery of cancer biomarkers”, GlaxoSmithKline, March 10, King of Prussia, PA 2009 Invited speaker “Prevention of ovarian cancer peritoneal metastasis by designing blocking strategies of CA125/mesothelin interaction using natural or recombinant antibodies.” 6th Cancer Drugs R&D Conference. February 19-20, Philadelphia, PA. 2009 Invited speaker “The coming of age of ovarian cancer biomarkers.” 2nd Oncology Biomarkers Conference. Jan. 19-20, Miami, FL. 2008 Invited speaker “Using yeast for display and secretion of recombinant antibodies”, LifeSensors, Inc. October 28, Malvern, PA. 2008 Invited speaker “Selection of high affinity anti-TEM1 scFv by magnetic and flow sortings of a novel yeast- display scFv library”, Bracco Research USA Inc, September 25, Princeton, NJ. 2008 Invited speaker “Yeast-display scFv and biobodies”, Morphotek, Inc., July 14, Exton, PA. 2008 Invited speaker “Generation of libraries of recombinant llama heavy-chain for diagnostic and discovery of cancer markers”. Second International Conference on Ovarian Cancer: State of the Art and Future Directions, June 26 - July 1, Rhodes, Greece 2008 Poster presentation “Generation of recombinant libraries of heavy-chain V regions derived from Llama Glama”. Scholler N., Gross J.A. and Urban N. Canary Foundation Early Detection Symposium, May 21-22, Stanford, CA. 2007 Oral presentation “Development and in vitro Validation of anti-Mesothelin Biobodies that prevent CA125/Mesothelin-dependent Cell Attachment.”. Bergan L, Gross JA, Nevin B, Urban N and Scholler N. 15th SPORE Investigators’ Workshop Concurrent Poster Discussion

4 Session: New Technologies and Methodologies in Translational Research II, July 7-10, Baltimore, MA. 2007 Poster presentation “Development and in vitro validation of anti-mesothelin biobodies that prevent CA125/Mesothelin-dependent cell attachment.” Scholler N, Bergan L, Gross JA, Garvik B, Nevin B, and Urban N. Federation of Clinical Immunology Societies (FOCIS) annual meeting, June 7-11, San Diego, CA. 2007 Poster presentation “Generation of a new type of yeast-secreted biotinylated recombinant antibodies directed against mesothelin and able to prevent CA125/mesothelin-dependent cell attachment.”. Scholler N, Garvik B, Bergan L, Nevin B, Gross JA, and Urban N. ASGT 10th Annual Meeting, May 30 - June 3, Seattle, WA. 2007 Invited speaker “Design of new classes of recombinant antibodies and validation for use in cancer diagnostics and therapy.” University of Pennsylvania May 16, Philadelphia, PA. 2007 Poster presentation “Validation of in vivo-biotinylated yeast-secreted recombinant antibodies for use in diagnostic tests.” Scholler N, Lowe K, Bergan L, Kampani A, Gross JA, Garvik B and Urban N. - BioMarker World Congress 2007, May 15–17, Philadelphia, PA. 2006 Invited speaker “Utilisation d'un test d'adhésion cellulaire basé sur l'interaction CA125/mésotheline pour tester in vitro de nouvelles voies d'approches thérapeutiques pour la prévention de micro- métastases péritonéales du cancer de l'ovaire.” Institut de Cancérologie et d'Immunologie de Marseille IFR 137 (Institute of Cancer and Immunology) INSERM UMR 599, November 15, Marseille, France. 2006 Invited speaker “Method for generation of in vivo biotinylated recombinant antibodies by yeast mating.” Laboratoire Technologies Avancées pour le Génome et la Clinique TAGC (Laboratory of Advanced Technology for Genome and Clinic) ERM 0206, November 14, Marseille – Luminy, France. 2006 Invited speaker “Generation of antigen-specific recombinant antibodies in the absence of purified and/or immunogenic antigens.” Seattle Biomedical Research Institute, November 6, Seattle, WA. 2006 Invited speaker “Generation of New Tools for Early Detection of Ovarian Cancer.” Complex Carbohydrate Research Center, University of Athens. September 29, Athens, Georgia. 2006 Oral presentation “Bead-Based ELISA Assays for Validation of Ovarian Cancer Early Detection Markers.” - Scholler N, Crawford M, Sato A, Drescher CW, O’Briant KC, Kiviat N, Anderson GL and Urban ND. Sixth Biennial Symposium sponsored by the MRCOCR, September 7-8, Seattle, WA. 2006 Oral presentation “Development of a CA125-Mesothelin Cell Adhesion Assay as a Screening Tool for Biologics Discovery.” Scholler N, Garvik B, Hayden-Ledbetter M, Kline T and Urban ND. Fourteenth SPORE Investigators’ Workshop Concurrent Plenary session, July 16-19, Baltimore MA. 2006 Poster presentation. “Biobodies: generation and validation.” Scholler, N. and Garvik, B. American Association of Immunologists, May 12–16, Boston, MA. 2005 Oral Presentation “Sandwich test ELISA with scFv antibodies: an alternative to an all-time favorite”- Scholler N, Garvik B and Urban N. Symposium of the Era of Hope Department of Defense Breast Cancer Research, Philadelphia, PA. 2004 Poster presentation “Generating New Tools for CD83 Functional Studies: Identification of an Anti-CD83 Recombinant Antibody that Binds to an HB15-Alternate Epitope” Hamel Y, Greenberg P and Scholler N. Twelfth International Congress of Immunology, Montreal, Canada. 2004 Oral presentation “Isolation of ovarian carcinoma-specific native proteins for development of new biomarkers.” Scholler N, Lampe P and Urban N. Eleventh SPORE Investigators Workshop, Baltimore, MD. 2002 Oral presentation “CD83 regulates the development of cellular immunity”. Scholler N, Hayden-Ledbetter M, Dahlin A, Hellström I, Hellström KE and Ledbetter JA. FASEB Immunology, New Orleans, LA. 2001 Oral presentation “CD83 is a adhesion receptor that binds monocytes and activated CD8+T cells” Scholler N, Disis ML, Dahlin A, Pullman J, Hellström KE and Hellström I. Eleventh International Congress of Immunology, Stockholm, Sweden. 2001 Oral presentation “HER2 DNA immunization with costimulatory molecules elicits functional anti-HER2 antibodies and CD4+ T cell tumor infiltration.” Scholler N, Ledbetter M., Hellström KE, Page 5 Hellström I, Ledbetter JA - Eleventh International Congress of Immunology, Stockholm, Sweden. 2001 Oral presentation “HER2 DNA immunization with costimulatory molecules elicits functional anti-HER2 Antibodies.”. Scholler N, Disis ML, Dahlin A, Pullman J, Hellström KE and Hellström I - Fourth Annual Meeting of the ASGT, Seattle, WA. Abstract published in Molecular Therapy. 3(5): S69-70. 2001 Poster presentation “HER2 DNA immunization with costimulatory molecules”. Scholler N, Disis ML, Dahlin A, Pullman J, Hellström KE and Hellström I. Ninth SPORE Investigators Workshop, Washington, DC. 2000 Oral presentation “Antibodies to the HER2 protein can be elicited with DNA immunization.” Scholler N, Disis ML, Pullman J, Hellström KE and Hellström I - Third Biennial Ovarian Cancer Research Symposium, Seattle, WA. 1998 Oral presentation “DNA immunization with a combination of Rat Neu antigen, CD86 and 4-1BB ligand results in the generation of HER-2/neu (HER2) antibodies in Neu transgenic (neuT) mice.” Scholler N, Shiota FM, Pullman J, Hellström KE, Hellström I and Disis ML. Seventh International Conference on Gene Therapy of Cancer, Coronado, CA. 1994 Poster presentation “Mapping of CTLA4-CD28 genomic region by yeast artificial chromosomes (YAC).” Buonavista N, Balzano C, Pontarotti P, Le Paslier D and Goldstein P.- May 11-15, Workshop Genome mapping and sequencing, Cold Spring Harbor, NY. 1992 Invited speaker “Interest and advantage of YAC technology for genome mapping”- September 15, Workshop Pulse Field Electrophoresis, Pharmacia, France. Abstracts 2011 “Blocking B7-H4- mediated T-cell inhibition to alter the tumor microenvironment”, Denarda Dangaj, Shree Joshi, Aizhi Zhao, Raphael Sandaltzopoulos and Nathalie Scholler. Poster presentation, 26th Annual Meeting of the Society for Immunotherapy of Cancer (SITC), November 4-6, 2011, Bethesda, MD. 2011 “Characterization of a TEM1 tumor-targeting antibody”. George Coukos, Aizhi Zhao, Chunsheng Li, Daniel Ricklin, Tamara Seedial, and Nathalie Scholler, Era of Hope 2011, Orlando, FL. 2011 “Developing magnetic nanoparticles for targeted nucleic acid delivery and detection” Poster presentation. Prantner A.M., Chen J., Murray C.B., and Scholler N. ASGCT 2011, May7 17-21, Seattle, WA. 2011 “B cells infiltrating ovarian cancer exhibit a regulatory-like phenotype.” Selene Nunez-Cruz, William J. Quinn III, Denise C. Connolly and Nathalie Scholler. Oral presentation, The Journal of Immunology, 2011 supplement, AAI 2011, May 13-17, San Francisco, CA. Travel Award, Selene Nunez-Cruz. 2011 “Mannose receptor regulates macrophage polarization in the tumor microenvironment” Denarda Dangaj, Shree Joshi, Aizhi Zhao, Raphael Sandaltzopoulos and Nathalie Scholler. Poster presentation, The Journal of Immunology, 2011 supplement, AAI 2011, May 13-17, San Francisco, CA. 2011 “Role of TLR engagement on TIL-B and clinical implications for ovarian cancer.” Selene Nunez-Cruz, Matthew Guerra, Denise Connolly, Phyllis Gimotty and Nathalie Scholler. Poster presentation, AACR 2011, April 2-5, Orlando, FL. 2011 “Developing targeted superparamagnetic iron oxides for combination chemohyperthermia” Prantner A.M., Chen J., Murray C.B., and Scholler N. Poster presentation, Immunochemotherapy: Correcting Immune Escape in Cancer, March 10, Philadelphia, PA. 2011 “Modeling the human tumor-microenvironment in CD34 transplanted NSG mice” Marc Walter, Selene Nunez- Cruz, Ananda Mookerjee, Chongyun Fang, Andrea Facciabene, George Coukos, Gwenn Danet-Desnoyers and Nathalie Scholler. Poster presentation, Immunochemotherapy: Correcting Immune Escape in Cancer, March 10, Philadelphia, PA. 2011 “Reconstitution of the human ovarian tumor-microenvironment in CD34-transplanted NOD-Scid-IL2Rc null (NSG) mice.” Marc Walter, Ananda Mookerjee, Selene Nunez-Cruz, Chongyun Fang, Andrea Facciabene, Daniel J. Powell Jr., George Coukos, Nathalie Scholler, and Gwenn Danet-Desnoyers. Poster presentation, TRC3 2011, February 24-26, Seven Springs, PA 2011 “Modulation of the tumor microenvironment by targeting the co-inhibitory B7H4 molecule”. Denarda Dangaj, Shree Joshi, Aizhi Zhao, Daniel J. Powell Jr., Raphael Sandaltzopoulos and Nathalie Scholler. Poster presentation, TRC3 2011, February 24-26, Seven Springs, PA 2010 “Characterization of scFv78: an anti-TEM1 scFv” Aizhi Zhao, Chunsheng Li, Daniel Ricklin, Tamara Seedial, George Coukos, Nathalie Scholler. International Society for Biological Therapy of Cancer (iSBTc). Poster 6 presentation, October 2 - 4, Washington, D.C. The Journal of Immunotherapy, in press. 2010 “Targeting of superparamagnetic iron oxide nanoparticles for cancer therapy based on localized hyperthermia” Andrew M. Prantner, Jun Chen, Christopher B. Murray and Nathalie Scholler. 6th annual Symposium Center for Translational Medicine, oral and poster presentations, June 4, Jefferson Medical College, Philadelphia, PA 2010 “The binding of mannose receptor by tumor-released GPI-anchor proteins controls tumor-associated macrophage phenotype”, Denarda Dangaj, Selene Nunez-Cruz, Aizhi Zhao, Raphael Sandaltzopoulos and Nathalie Scholler. Oral presentation, CRRWH annual retreat, University of Pennsylvania, PA. Presentation winner, D. Dangaj. 2010 “Gold nanorods conjugated to tumor-specific antibody for the targeting of ovarian cancer” Kolin C. Hribar, Gregory R. Wiedman, Andrew Prantner, Chaitanya Divgi, and Nathalie Scholler. Senior Design project of undergraduate students in Bioengineering, poster presentation, April 20, University of Pennsylvania, School of Engineering, PA. 2010 “Ovarian tumor infiltrating lymphocytes B (TIL-B) present pro-angiogenic properties.” Selene Nunez-Cruz, Matthew Guerra, Denise Connolly, George Coukos, Phyllis Gimotty and Nathalie Scholler - The Journal of Immunology, 2010, 184, 101.7; AAI 2010, Poster and oral presentations. May 7-10, Baltimore, MD. Trainee Achievement Award, Selene Nunez-Cruz. 2010 “Comparative screening of human antibody libraries derived from autoimmune vs. cancer patients for affinity reagents directed against tumor-associated antigens”. Aizhi Zhao, Yi Cheng, George Coukos, Don Siegel, and Nathalie Scholler. - The Journal of Immunology, 2010, 184, 101.20 AAI 2010, Poster presentation. May 7-10, Baltimore, MD. 2010 “Tumor-released GPI-anchored proteins bind to mannose receptor and polarize tumor-infiltrating macrophages.” Denarda Dangaj, Aizhi Zhao, Selene Nunez-Cruz, Raphael Sandaltzopoulos and Nathalie Scholler - AACR 2010 Poster presentation. April 20, Washington, DC. 2010 “Genetic and pharmaceutical approaches for the study of complement factors as alternative therapy for ovarian cancer”. Selene Nunez-Cruz, Hongchang Qu, Stavros Rafail, Denise Connolly, Phyllis Gimotty, John Lambris, George Coukos, and Nathalie Scholler. - AACR 2010 Poster presentation. April 19, Washington, DC. 2010 “In vivo blocking of CA125/mesothelin-dependent cell adhesion prevents ovarian cancer peritoneal metastasis”. Denarda Dangaj, Amber Roberts, Raphael Sandaltzopoulos and Nathalie Scholler. Poster presentation.Society of Gynecologic Oncologists (SGO) 41st Annual Meeting on Women's Cancer. March 14-17, San Francisco, CA. 2010 “MTB Hsp70-scFv Fusion and Self Assembling Proteins Targeting Mesothelin Control Tumor Growth and Augment Tumor Specific T Cell Responses In A Murine Model of Ovarian Cancer” Jianping Yuan, Pierre Leblanc, Elda Righi, Svetlana Korochkina, Jacalyn Rosenblatt, Brett Gotzbecker, Dimitris Tzachanis, Michael Santosuosso, Sandra Orsulic, Heidi Mills, Nathalie Scholler, David Avigan, Jeff Gelfand and Mark C. Poznansky. Poster presentation. Symposium Dana Farber/ Harvard Cancer Center ovca/ breast ca meeting. March 5, 2010, Boston, MA. 2010 “Ovarian tumor infiltrating lymphocytes B (TIL-B) are capable of synthesis and secretion of both pro- and anti- angiogenic factors.” Selene Nunez-Cruz, Matthew Guerra, Denise Connolly, George Coukos, Phyllis Gimotty and Nathalie Scholler - Poster presentation, 13th Annual Meeting of the Translational Research Cancer Centers Consortium conference, The Ohio State University - March 1-2- Colombus, OH- First Price poster winner. 2010 “The binding of mannose receptor by tumor-released GPI-anchor proteins controls tumor-associated macrophage phenotype” Authors: Denarda Dangaj, Selene Nunez-Cruz, Aizhi Zhao, Raphael Sandaltzopoulos and Nathalie Scholler. Poster presentation. 13th Annual Meeting of the Translational Research Cancer Centers Consortium conference, The Ohio State University - March 1-2- Colombus, OH. 2009 “Development of a screening strategy to identify affinity reagents against formalin-fixed antigen”. Natalia Diaz- Rodriguez, Aizhi Zhao, Nathalie Scholler. Poster presentation. Short-term Elective Research (STER) Summer Student Symposium – September 11 – University of Pennsylvania, Philadelphia, PA. 2009 “Dual Specificity Suicide Gene Therapy Using Targeted Nanoparticles Directed against Ovarian Cancer”. Andrew M. Prantner, Patricia Ohana, Abraham Hochberg, Jerry D. Glickson, George Coukos and Nathalie Scholler. Poster presentation. Biomedical Post Doctoral Research Symposium 2009. University of Pennsylvania, Philadelphia, PA. 2009 “Tumor infiltrating-B cells (TIL-B) exhibit a regulatory phenotype in a transgenic mouse model of serous ovarian cancer”. Selene Nunez-Cruz, Matthew Guerra, Denise Connolly, George Coukos, Phyllis Gimotty and Nathalie Scholler. Poster presentation. Biomedical Post Doctoral Research Symposium 2009. University of Pennsylvania, Philadelphia, PA. 2009 “Development and validation of a ScFv specific for human glypican-3”. Yonghai Li, Aizhi Zhao, Hong Li, Oluwasayo Adeyemo, Nathalie Scholler, David Kaplan. Poster presentation. Department of Medicine Research Page 7 Day - June 5 - University of Pennsylvania, Philadelphia, PA. 2009 “Construction of a large scFv yeast display library and isolation of TEM1-specific antibodies of high affinity for targeted-imaging and therapy of cancer” Zhao ., Li C., Nunez-Cruz S., Prantner A.M., Coukos G., Siegel D.L. and Scholler N. Poster presentation. CRRWH annual retreat, University of Pennsylvania - Philadelphia, PA. 2009 “Rescue of anti-tumor macrophages using mannose-receptor targeted recombinant antibodies for ovarian cancer therapeutics” Dangaj D., Sandaltzopoulos R., Zhao A., Coukos G. and Scholler N. Poster presentation. CRRWH annual retreat, University of Pennsylvania - May 27- Philadelphia, PA. 2009 “Developing a universal method to prepare scFv-modified nanoparticles for ovarian cancer diagnosis and therapy”. Prantner A.M., Glickson J.D., Coukos G. and Scholler N. Key note presentation. CRRWH annual retreat, University of Pennsylvania - May 27- Philadelphia, PA. 2009 “Development of an orthotopic mouse model of serous ovarian cancer for in vivo imaging” Nunez-Cruz S., Prantner A.M., Zhao A., Roberts A., Secreto A., Connolly D., Danet-Denoyers G., Coukos G. and Scholler N. Poster presentation. CRRWH annual retreat, University of Pennsylvania - May 27- Philadelphia, PA. 2009 “Genetic Approach to Study Complement Role in Ovarian Cancer”. Nunez-Cruz S., Connolly D., Roberts A., Lambris J.D., Coukos G. and Scholler N. The Journal of Immunology, 2009, 182, 88.24; AAI 2009 - Poster or oral presentations. -May 8-12 - Seattle, WA. Travel Award, Selene Nunez-Cruz. 2009 “Identification of high affinity scFv antibodies against mouse and human tumor endothelial marker 1 (TEM1) using yeast-display scFv library derived from autoimmune patients”. Zhao, A, Li, C., Coukos C, Siegel D and Scholler N. The Journal of Immunology, 2010, 184, 101.20; AAI 2009- Poster or oral presentations. May 8-12 - Seattle, WA. Travel Award, Aizhi Zhao. 2009 “Genetic Approach to Study Complement Role in Ovarian Cancer”. Nunez-Cruz S., Connolly D., Roberts A., Lambris J.D., Coukos G. and Scholler N. Poster presentation. 12th Annual Meeting of the Translational Research Cancer Centers Consortium conference, University of Pennsylvania- March 6-7- Philadelphia, PA Third Price poster winner. 2009 “Blocking the chemical crosstalk between tumor cells and macrophages with recombinant antibodies to trigger tumor immune rejection.” Dangaj D, Sandaltzopoulos R, Coukos G and Scholler N. Poster presentation. 12th Annual Meeting of Translational Research Cancer Centers Consortium conference, University of Pennsylvania- March 6-7- Philadelphia, PA 2009 “Development of a universal method for targeting nanoparticles with functionally intact scFv”. Prantner A.M., Glickson J.D., Coukos G and Scholler N. 12th Annual Meeting of the Translational Research Cancer Center s Consortium conference, Poster presentation University of Pennsylvania- March 6-7- Philadelphia, PA 2008 “In vivo Modeling and Detection of the Ovarian Cancer Vascular Marker TEM1”. Li C., Swails JC, Hasegawa K, O'Brien-Jenkins A, Liu Y, El-Deiry WS, Scholler N, Divgi C, Gimotty PA, Grasso L, Coukos G –Third International Conference on Molecular Diagnostics in Cancer Therapeutic Development, Poster and oral presentations. AACR - September 22, 2008- Philadelphia, PA. Award winner. 2008 “Novel Tumor Vascular Markers: Useful Biomarkers and Therapeutic Targets in Epithelial Ovarian Cancer”. Sasaroli D., Kougioumtzidou E., Hammond R., Buckanovich R.J., Sandaltzopoulos R., Scholler N., Gimotty P.A. and Coukos G. Poster presentation Second International Conference on Ovarian Cancer: State of the Art and Future Directions - June 26-July 1, 2008 - Rhodes, Greece 2008 “The Influence of Ovarian Cancer Risk-Status on the Diagnostic Performance of the Biomarkers Mesothelin, HE4 and CA125.” Shah C., Lowe K., Paley P., Wallace E., Anderson G., McIntosh M., Andersen R., Scholler N., Bergan L., Thorpe J., Urban N., Drescher C. Poster presentation Joint Meeting of the Western Association of Gynecologic Oncologists (WAGO) and the Felix Rutledge Society (FRS) - June 11-14, 2008 - Fairmont Sonoma Mission Inn, Sonoma, CA. 2008 “HE4 Bead-Based ELISA: Use of yeast-secreted in vivo biotinylated recombinant antibodies (Biobodies).” Forrest R., Scholler N., Lowe K., Bergan L., Kampani A., Thorpe J., Gross J., Garvik B. and Urban N. - Poster presentation. Canary Foundation Early Detection Symposium, May 21-22, Stanford, CA. 2008 “Use of cancer-specific in vivo-biotinylated recombinant antibodies (Biobodies) for serum biomarker discovery.” Scholler N., Gross J.A., Garvik B., Wells L., Liu Y., Loch C.M., Ramirez A.B., McIntosh M.W., Lampe P.D. and Urban N. Poster presentation. Canary Foundation Early Detection Symposium, May 21-22, Stanford, CA. 2008 “Vascular Proteins Predict Clinical Outcome in Ovarian Cancer.” Sasaroli D., Gimotty P.A., Hammond R., Seedial T., Buckanovich R.J., Katsaros D., Scholler N. and Coukos G. Poster presentation. Canary Foundation Early Detection Symposium, May 21-22, Stanford, CA

8 2005 “Use of a yeast library of human antibody recognition sequences to develop diagnostic reagents.” Scholler N, Garvik B and Urban N. Poster presentation. XXII International Conference on Yeast Genetics and Molecular Biology, Bratislava, Slovakia. Peer-reviewed research articles (*corresponding author) 1. Buonavista, N., Balzano, C., Pontarotti, P., Le Paslier, D. and Goldstein, P. 1992. Molecular linkage of human CTLA-4 and CD28 Ig-superfamily genes in yeast artificial chromosomes. Genomics. Jul; 13(3):856-861. 2. Balzano, C., Buonavista, N., Rouvier, E. and Goldstein, P. 1992. CTLA-4 CD28, similar protein, neighboring genes. International Journal of Cancer. Suppl 7, 28-32. 3. Cornillon, S., Foa, C., Davoust, J., Buonavista, N., Gross, J. D. and Goldstein, P. 1994. Programmed cell death in Dictyostelium. Journal of Cell Science. Oct; 107(10): 2691-2704. 4. Couissiner-Paris, P., Bourgeois, A., Dessein, H., Bacellar, O., Rodriguez, V. Jr., Kohlstadt, S., Buonavista, N., Pene, J. and Dessein, A. J. 1995. Identification of a major T cell immunogen in the anti-schistosome response of adult residents in an area endemic of Schistosoma mansoni. European Journal of Immunology. 24 (4): 903-910. 5. Scholler, N., Fu, N., Yang, Y., Ye, Z. M., Goodman, G. E., Hellström, K. E. and Hellström, I. 1999. Soluble member(s) of the mesothelin/megakaryocyte potentiating factor family are detectable in sera from patients with ovarian carcinoma. Proceedings of the National Academy of Sciences of the United States of America. 96(20):11531-11536. 6. Hellström, I., Ledbetter, J. A., Scholler, N., Yang, Y., Ye, Z. M., Goodman, G. E., Pullman, J., Hayden-Ledbetter, M. and Hellström, K. E. 2001. CD3-mediated activation of tumor-reactive lymphocytes from patients with advanced cancer. Proceedings of the National Academy of Sciences of the United States of America. Jun 5; 98(12): 6783-6788. 7. *Scholler, N., Hayden-Ledbetter, M., Hellström, I., Hellström, K. E. and Ledbetter, J. A. 2001. CD83 is a Sialic Acid-Binding Immunoglobin-Like Lectin (Siglec) Adhesion Receptor that Binds Monocytes and a Subset of Activated CD8+ T Cells. Journal of Immunology. 166: 3865-3872. 8. * Scholler, N., Hayden-Ledbetter, M., Dahlin, A., Hellström, I., Hellström, K. E. and Ledbetter, J. A. 2002. CD83 Regulates the Development of Cellular Immunity. Journal of Immunology. 168: 2599-2602. 9. Disis, M. L.+, Scholler, N.+, Dahlin, A., Pullman, J., Knutson, K. L., Hellström, K. E. and Hellström, I. 2003. Plasmid-based vaccines encoding rat neu and immune stimulatory molecules can elicit rat neu-specific immunity. Molecular Cancer Therapy. Oct; 2(10):995-1002.(+these authors equally contributed to the work). 10. McIntosh, M. W., Drescher, C., Karlan, B., Scholler, N., Urban, N., Hellström, I. and Hellström, K. E. 2004. Combining CA 125 and SMR serum markers for diagnosis and early detection of ovarian carcinoma. Gynecologic Oncology. 95(1):9-15. 11. *Scholler, N., Crawford, M., Sato, A., Drescher, C. W., O’Briant, K. C., Kiviat, N., Anderson, G. L. and Urban, N. Bead-based ELISA assays for validation of ovarian cancer early detection markers. Clinical Cancer Research. 2006. 12: 2117-2124. 12. *Scholler, N., Garvik, B., Quarles, T., Jiang, S. and Urban, N. 2006. Method for generation of in vivo biotinylated recombinant antibodies by yeast mating. Journal of Immunological Methods. Dec 20; 317(1-2):132-143. Epub 2006 Oct 30. 13. *Scholler, N., Garvik, B., Hayden-Ledbetter, M., Kline, T. and Urban, N. 2007. Development of a CA125- Mesothelin cell adhesion assay as a screening tool for biologics discovery. Cancer Letters. Mar 8; 247(1):130- 136. Epub 2006 May 4. 14. Bergan, L., Gross, J. A., Nevin, B., Urban, N., and *Scholler, N. 2007. Development and in vitro validation of anti-mesothelin biobodies that prevent CA125/Mesothelin-dependent cell attachment. Cancer Letters. Oct 8; 255(2):263-74. Epub 2007 Jun 7. 15. Loch, C. M., Ramirez, A. B., Liu, Y., Sather, C. L., Delrow, J.J., Garvik, B., Scholler, N., Urban, N., McIntosh, M. W. and Lampe, P. D. Use of High Density Antibody Arrays to Validate and Discover Cancer Serum Biomarkers. Molecular Oncology. Volume 1, Issue 3, December 2007, Pages 313-320 16. * Scholler, N., Lowe, K. A., Bergan, L. A., Kampani, A. V., Ng, V., Forrest, R. M., Thorpe, J. D., Gross, J. A., Garvik, B. M., Drapkin, R., Anderson, G. L. and Urban, N. Use of yeast-secreted in vivo biotinylated recombinant antibodies (biobodies) in bead–based ELISA. Clinical Cancer Research. 2008 May 1:14(9):2647-55 17. Faca, V., Song, K., Wang, H., Zhang, Q., Krasnoselsky, A., Ireton, R., Newcomb, L., Plentz, R., Glukhova, V., Phanstiel, D., Gurumurthy, S., Redston, M. S., Brenner, D., Anderson, M., Misek, D., Scholler, N., Urban, N., Barnett, M., Edelstein, C., Goodman, G., Thornquist, M., McIntosh, M., DePinho, R. A., Bardeesy, N. and

Hanash S. Markers for human pancreatic cancer based on plasma proteome analysis of a mouse model. PLoS Med. 2008 Jun 10;5(6):e123. 18. Palmer C., Duan X., Hawley S., Scholler N., Thorpe J., Sahota R., Wong M., Wray A., Drescher C., McIntosh M., Brown P., Nelson B., Urban N. Systematic Evaluation of Candidate Blood Markers for Detecting Ovarian Cancer. PLoS ONE - 2008 Jul 9;3(7):e2633. 19. Andersen MR, Goff BA, Lowe KA, Scholler N, Bergan L, Dresher CW, Paley P, Urban N. Combining a symptoms index with CA 125 to improve detection of ovarian cancer. Cancer. 2008 Jun 25. Jun 25;113(3):484- 489. DOI: 10.1002/cncr.23577 20. *Scholler N., Gross JA., Garvik B., Wells L., Liu Y., Loch CM., Ramirez AB., McIntosh MW., Lampe PD., Urban N. Use of cancer-specific yeast-secreted in vivo biotinylated recombinant antibodies for serum biomarker discovery. Journal of Translational Medicine. 2008, 6:41 21. Lowe K., Shah C., Wallace E., Anderson G., Paley P., McIntosh M., Andersen R., Scholler N., Bergan L., Thorpe J., Urban N., Drescher C. Effects of Personal Characteristics on Serum CA125, Mesothelin, and HE4 Levels in Healthy Post-menopausal Women at High-Risk for Ovarian Cancer. Cancer Epidemiology, Biomarkers & Prevention. 2008 Sep;17(9):2480-7. 22. C. Shah, K. Lowe, P. Paley, E. Wallace, G. Anderson, M. McIntosh, R. Andersen, N. Scholler, L. Bergan, J. Thorpe, N. Urban, C. Drescher. “Influence of ovarian cancer Risk status on the Diagnostic Performance of the serum Biomarkers Mesothelin. HE4 and CA125”.-Cancer Epidemiology Biomarkers & Prevention (CEBC) Prev. 2009 May;18(5):1365-72 23. Gonzalez, J.M., Ofori E., Burd I., Chai J., Scholler N., Elovitz M.A.: “Maternal mortality from systemic illness: unraveling the contribution of the immune response”. Am J Obstet Gynecol 2009, 200, 430 e1-8. 24. Chen, A., Kozak, D., Battersby, B.J., Forrest, R.M., Scholler, N., Urban, N., and Trau, M. “Antifouling Surface Layers for Improved Signal-to-Noise of Particle-Based Immunoassays” –Langmuir. 2009, 25 (23), pp 13510– 13515 25. Anderson G.L., McIntosh M.,Wu L., Thorpe J.D., Bergan L., Goodman G., Barnett M., Thornquist M.D., Scholler N., Kim N., O’Briant K., Drescher C., Urban N.. “Assessing Lead Time of Selected Ovarian Cancer Biomarkers: A Nested Case-Control Study” –JNCI. 2010 Vol. 102, Issue 1, January 6. 26. Andersen MR, Goff BA, Lowe KA, Scholler N, Bergan L, Drescher CW, Paley P and Urban N. “Use of a Symptom Index, CA125, and HE4 to predict ovarian cancer.” -Gynecol Oncol. 2010 Mar;116(3):378-83. Epub 2009 Nov 28. 27. Aizhi Zhao, Selene Nunez-Cruz, Chunsheng Li, George Coukos, Don L. Siegel and *Nathalie Scholler. “Rapid isolation of high affinity human antibodies against the tumor vascular marker Endosialin/TEM1, using a paired yeast display/secretory scFv library platform.” JIM, Jan 5; 363(2):221-32. Epub 2010 Sep 15. 28. Selene Nunez-Cruz, Denise C. Connolly and Nathalie Scholler. “An orthotopic model of serous ovarian cancer in immunocompetent mice for in vivo tumor imaging and monitoring of tumor immune responses.” JoVE , 2010 Nov 28. (45), pii:2146, doi:10.3791/2146. [Epub ahead of print] 29. Zhu CS, Pinsky PF, Cramer DW, Ransohoff DF, Hartge P, Pfeiffer RM, Urban N, Mor G, Bast RC Jr, Moore LE, Lokshin AE, McIntosh MW, Skates SJ, Vitonis A, Zhang Z, Ward DC, Symanowski JT, Lomakin A, Fung ET, Sluss PM, Scholler N, Lu KH, Marrangoni AM, Patriotis C, Srivastava S, Buys SS, Berg CD; PLCO Project Team. A framework for evaluating biomarkers for early detection: validation of biomarker panels for ovarian cancer. Cancer Prev Res (Phila). 2011 Mar;4(3):375-83. 30. Cramer DW, Bast RC Jr, Berg CD, Diamandis EP, Godwin AK, Hartge P, Lokshin AE, Lu KH, McIntosh MW, Mor G, Patriotis C, Pinsky PF, Thornquist MD, Scholler N, Skates SJ, Sluss PM, Srivastava S, Ward DC, Zhang Z, Zhu CS, Urban N. Ovarian cancer biomarker performance in prostate, lung, colorectal, and ovarian cancer screening trial specimens. Cancer Prev Res (Phila). 2011 Mar;4(3):365-74. 31. Sasaroli D, Gimotty PA, Pathak HB, Hammond R, Kougioumtzidou E, Katsaros D, Buckanovich R, Devarajan K, Sandaltzopoulos R, Godwin AK, Scholler N, Coukos G. Novel surface targets and serum biomarkers from the ovarian cancer vasculature. Cancer Biol Ther. 2011 Aug 1;12(3). [Epub ahead of print] PubMed PMID: 21617380 32. Nicole Urban, Jason D. Thorpe, Martin McIntosh, Lindsay Bergan, Robin Forrest, Archana Kampani, Nathalie Scholler, Kathy O’Briant, Charles Drescher, Dan Cramer, Chris Berg. "Potential role of HE4 in multimodal screening for epithelial ovarian cancer" JNCI brief communication 2011; 103:1-5 [Epub ahead of print.] 33. Eleftheria Kalogera; Nathalie Scholler; Cecelia Powless; Amy Weaver; Ronny Drapkin; Jinping Li; Shi-Wen Jiang; Karl Podratz; Nicole Urban; Sean Christopher Dowdy. “Correlation of Serum HE4 with Tumor Size and Myometrial Invasion in Endometrial Cancer”. Gynecologic Oncology [online October 28, 2011] 10 34. Evripidis Lanitis, Mathilde Poussin, Ian Hagemann, Raphael Sandaltzopoulos, Nathalie Scholler, and Daniel J. Powell, Jr. “Redirected anti-tumor activity of primary human lymphocytes transduced with a fully-human anti- mesothelin chimeric receptor”. Molecular Therapy [Online Nov 29, 2011] 35. Denarda Dangaj, Karen Abbott, Ananda Mookerjee, Aizhi Zhao, Pamela S. Kirby, Raphael Sandaltzopoulos, Daniel J. Powell Jr., Antonin Lamazière, Don L. Siegel, Claude Wolf, and *Nathalie Scholler. “Mannose receptor engagement by mesothelin GPI anchor polarizes tumor associated macrophages and is blocked by a recombinant antibody directed against mannose receptor domain 4.” PLos One [Online Dec 6, 2011]

Submitted peer-reviewed research articles. Yonghai Li, Donald L Siegel, Nathalie Scholler and David E Kaplan. “Glypican-3-specific scFv isolation and validation for use in hepatocellular carcinoma”. Katarzyna Urbanska, Evripidis Lanitis, Mathilde Poussin, Rachel Lynn, Brian P Gavin, Sander Kelderman, Jason Yu, Nathalie Scholler and Daniel J Powell Jr. “A novel immune receptor expressed by T cells for universal targeting of diverse and multiple tumor associated antigens”. Accepted pending minor revisions. Cancer Research.

Reviews *Scholler, N. and Urban N. CA125 in Ovarian Cancer. Biomarkers in Medicine 1(4), December 2007, Pages 513-523. *Scholler, N., Mesothelin, Encyclopedia of Cancer, 2nd edition, 1836-1940 (2008) *Scholler, N.,2nd Oncology Biomarkers Conference (Miami, FL), Thomson Reuters Scientific division (2009) *Scholler, N.6th Cancer Drugs R&D Conference (Philadelphia, PA), , Thomson Reuters Scientific division (2009) *Scholler N., “Oncology Biomarkers. the Investigational Drugs journal 2009 12(4): © Thomson Reuters (Scientific) Ltd ISSN 2040-3410 Sasaroli D., Coukos G. and *Scholler N. “Beyond CA125: the coming of age of ovarian cancer biomarkers. Are we there yet?” - Biomarkers in Medicine. Prev. 2009 May;18(5):1365-72 Tanyi J.L and *Scholler N. “From the endometrium physiology to a comprehensive strategy for the discovery of ovarian cancer biomarkers.” Oncology reviews. 2010 March, Vol. 4, Number 1, pages 43-50. *Scholler, N, 3rd Oncology Biomarkers Conference (San Diego, CA), Thomson Reuters Scientific division. (2011) Tanyi, J.L. and *Scholler, N. “Oncology Biomarkers for Detection and Therapeutic Monitoring of Gynecologic Malignancies”. Front Biosci (Elite Ed). 2012 Jan 1;4:1097-110.

Book Chapters Mantia-Smaldone, G.M. and *Scholler, N. "Ovarian Cancer" chapter of InTech book project, Open Access publisher. ISBN: 978-953-307-812-0. (in press) *Scholler, N. "Selection of antibody fragments by yeast display" chapter of “Antibody Engineering: methods and protocols" section in the lab-protocol based 'Methods in Molecular Biology' series, Humana Press editors. (in press) Nunez-Cruz, S. and *Scholler, N. "Immunocompetent mouse model of ovarian cancer for in vivo imaging" chapter of “Ovarian Cancer”, Humana Press editors (in press)

Editorial *Scholler, N. Novel targeting strategies using recombinant antibodies for early diagnosis and therapy of ovarian cancer. Future Medicine Therapy. 2010, 7(3), 209-212

Patents 1. Scholler N. and Garvik B., US Patent No. 7,795,411 (“Methods of Generation of in vivo biotinylated recombinant antibodies”). 2. Law CL, Pestano LA, Wahl AF, and Scholler N, US Patent No. 7,662,387 (“Anti-CD70 antibody-drug conjugates and their use for the treatment of cancer and immune disorders”). 3. Law CL, Pestano LA, Wahl AF, and Scholler N, US Patent Application No. 12/467,182 (“Anti-CD70 antibody- drug conjugates and their use for the treatment of cancer and immune disorders”).

4. Law CL, Pestano LA, Wahl AF, and Scholler N, US Patent Application No. 12/635,571 (“Treatment of B-cell cancers with anti-CD70 antibody drug conjugates”). 5. Law CL, Pestano LA, Wahl AF, and Scholler N, US Patent Application No. 12/635,607 (“Treatment of renal cell carcinoma with anti-CD70 antibody-drug conjugates”). 6. Scholler N, Disis ML, Hellström I, and Hellström KE, US Patent No. 6,734,172 (“Surface receptor antigen vaccines”). 7. Ledbetter JA, Scholler N, Hayden-Ledbetter M, Hellström I, and Hellström KE, US Patent Application No. 10/098,221 (“Compositions and methods to regulate an immune response using CD83 gene expressed in tumors and using soluble CD83-Ig fusion protein”). 8. Ledbetter JA, Scholler N, Hayden-Ledbetter M, Hellström I, and Hellström KE, US Patent Application No. 11/600,927 (“Compositions and methods to regulate an immune response using CD83 gene expressed in tumors and using soluble CD83-Ig fusion protein”). 9. Scholler N, Hellström I, and Hellström KE, US Patent No. 6,770,445 (“Methods and compositions for diagnosing carcinomas”). 10. Scholler N, Hellström I, and Hellström KE, US Patent No. 7,745,159 (“Methods and compositions for diagnosing carcinomas”). 11. Scholler N, Hellström I, and Hellström KE, US Patent Application No. 12/781,231 (“Methods and compositions for diagnosing carcinomas”). 12. Scholler N, Zhao, Siegel DL, and Coukos G, International Patent Application No. PCT/US10/56477 (“Anti- TEM1 antibodies and uses thereof”) 13. Powell DJ and Scholler N, A fully human, anti-mesothelin specific chimeric immune receptor for redirected mesothelin-expressing cell targeting. US provisional patent application No. 61/552,820, filed on October 28, 2011

12 Mentoring and teaching activities Received Training 2008 The Art of Speaking: Professional Communication for Faculty 2008 Effective Abstract Writing 2008 The Art of Award Winning Poster Presentations 2010 Overview of Education at Penn Medicine Workshop 2010 Writing Letters of Recommendation for Trainees 2010 Interactive Teaching 2011 Effective Lecturing 2012 Interpersonal Dynamics course

Administrative Teaching Activities Dates Position/title Organization Location Frequency Total hrs/yr Since 2008 IGG Recruitment Biomedical Graduate Group (IGG) Perelman School Annual 4 committee member of Medicine Since 2010 Judge and mentor Annual AACR Undergraduate Student AACR meetings Annual 8 Caucus and Poster Competition. 06/02/2010 Facilitator Responsible Conduct in Research (RCR) Perelman School Once 2 workshop of Medicine 04/06/2011 Mentor AACR, Special Program for High School AACR meetings Once 2 Students 05/19/2011 Committee member Biomedical Graduate Group, Cancer Perelman School Once 4 for Rafael Winograd Biology. Thesis Preliminary exam. of Medicine 07/18/2011 Committee member Bioengineering Graduate Group, Penn School of Once 4 for Ajlan Al Zaki Cellular and Molecular Imaging. Bioengineering Thesis Preliminary exam. Since 2011 Moderator Monday Night Journal Club, Immunology Perelman School of Annual 3 Medicine 2012 Thesis committee Biomedical Graduate Group, Cancer Perelman School Quarterly 20 member for Rafael Biology. of Medicine Winograd

Teaching Date Teaching type Title Location Type and number Frequency Total of students hrs/yr 2007 Workshop Recombinant Fred Hutchinson CRC, 30 Post-doctoral Once 2 antibodies Seattle, WA fellows 2008 Lecture Yeast-display scFv Cold Spring Harbor, NJ 40 Post-doctoral Once 2 fellows 2009 Lecture Introduction to innate Biostatistic Graduate 7 graduate students Once 2 and adaptive immune Group, Upenn. systems & inflammation 2009 Directed reading Role of B cells in solid Biomedical Graduate 1 graduate students Once 10 (Imm 520) tumor Group, Upenn. Natalie Steinel 2010 Lecture Targeted therapies Bioengineering Graduate 21 undergraduate Once 1 (CBE 564) Group, Upenn, students Since 2010 Lecture Immunology Faculty Biomedical Graduate ~15 graduate Annual 0.5 (Imm 599) Research Seminar Group, Upenn. students 2010 Lab rotation Yeast-display scFv Perelman School of 1 graduate student Once 12 (Imm 699) screening Medicine. Nicole Martinez

Since 2010 Course Tumor Biomedical Graduate 9 graduate Annual 30 (CAMB 701) microenvironment Group, Upenn. students 2011 Course Humoral responses Biomedical Graduate 4 graduate Once 36 (Imm 508) in cancer Group, Upenn. students 2011 Lab rotation Mannose receptor Biomedical Graduate 1 graduate student Once 12 (CAMB 699) polymorphism Group, Upenn. Michael Allegrezza 2012 Independent Role of B cells in Biomedical Graduate 1 graduate student Once 30 study (MD/PhD ovarian cancer Group, Upenn. Timothy Chao program)

Trainees Graduate students Date Position in my lab Name Publications, abstracts Current position and awards 2008-present PhD Student/ visitor Denarda Dangaj Publication: PhD Student/visitor scientist PlosOne 1st author scientist in my lab (PENN lab) Abstracts & Awards: SITC 2011; AAI 2011, JI supplement; Best presentation CRRWH 2010 Post-doctoral fellows 2005-2006 Post-doctoral Fellow Sanjiban Barnejee Founder of AbGenics, (FHCRC lab) National Chemical Laboratory, Pune, India 2008-2009 Post-doctoral Fellow Publications: (PENN lab) JIM 2010, 2nd author. JoVE 2010, 1st author. Abstracts & Awards: AAI 2009, Travel Selene Nunez-Cruz Award, JI suppl. AAI 2010, Trainee Research associate in Achievement award, my lab since April JI suppl. 2010. AAI 2011, Travel Award, JI suppl. Third price poster presentation TRC3 2009 First price poster presentation TRC3 2010 2008-2010 Post-doctoral Fellow Aizhi Zhao Publications: Research associate (PENN lab) JIM 2010, 1st author. since November 2010 PLos ONE 2011, 4th (Coukos lab, OCRC). author. Abstracts & Awards: AAI 2009, Travel Award, JI suppl. 2008-present Post-doctoral Fellow Andrew Prantner Abstracts & Awards: Post-doctoral Fellow (PENN lab) Keynote speaker, in my lab.

14 CRRWH

2010-2011 Post-doctoral Fellow Ananda Mookerjee Publication: PLos Post-doctoral Fellow (PENN lab) ONE 3rd author (Coukos lab, OCRC)

Mentoring 2012 Volunteer work Renyao Wei Undergrad. in Bioengineering, Penn School of Engineering 2012 Visiting Medical Student Carlo Fremd Medical Student, University of Heidelberg, Germany 2011 Summer Internship Norma A. Brown Undergrad. in Bioengineering, Penn School of Engineering 2010-11 Elizabeth Blackwell Society Katie Manning First year Medical school student, Penn School of Medicine 2010-11 Senior Design Project Andrew Lee Undergrad. in Bioengineering, Penn School of Engineering 2010-11 Senior Design Project Kevin Chu Undergrad. in Bioengineering, Penn School of Engineering 2010-11 Senior Design Project Matt Cha Undergrad. in Bioengineering, Penn School of Engineering 2010-11 Senior Design Project Xiao Ling Undergrad. in Bioengineering, Penn School of Engineering 2010 Summer Internship Ashley Jordan Undergraduate in Biochemistry, Special Programs Biomedical Graduate Studies, UPenn, PA. 2009-10 Elizabeth Blackwell Society Charlotte Lawson First year Medical school student, Penn School of Medicine 2009-10 Senior Design Project Kolin Hribar Undergrad. in Bioengineering, Penn School of Engineering. Now a graduate student at UC, San Diego, CA. 2009-10 Senior Design Project Gregory Wiedman Undergrad. in Bioengineering, Penn School of Engineering. Now a graduate student at John Hopkins, Baltimore, MA. 2009 Independent work study Tamara Seedial Masters in Biotechnology, Penn School of Engineering. 2009 Independent work study Natalia NIH-funded Short Term Training, PhD student, University Diaz-Rodriguez of Pennsylvania. 2008 Visitor scientist Murielle Saade Graduate student, University of Aix- Marseille II, France Now a post-doctoral fellow in Barcelona, Spain.