Online Resource 1 PCR and Sequencing Primers

Online Resource 3

Online Resource 3 Taxa included in the molecular analyses with NCBI GenBank accession numbers

Terminal taxa 18S 16S COI H3 Dorvillea erucaeformis (Malmgren, 1865) DQ779647 GQ478122 AY838868 --- Dorvillea rubrovittata (Grube, 1855) --- GQ415457 JQ310754 GQ415490 Eunice pennata (O.F. Müller, 1776) AY838849 AF321418 AY838870 DQ779731 Ophryotrocha lobifera Oug, 1978 --- GQ415464 GQ415481 GQ415500 Ophryotrocha orensanzi Taboada et al., 2013 --- KC123176 KC123178 KC123180 Parougia albomaculata (Åkesson & Rice, 1992) --- AF380115 EF464550 JQ310784 Parougia bermudensis (Åkesson & Rice, 1992) AF412802 JQ310753 JQ310767 JQ310785 Parougia eliasoni (Oug, 1978) --- GQ415470 GQ415489 GQ415507 Parougia oregonensis Hilbig & Fiege, 2001 --- JX536705 ------Parougia sp. Seep Clade CA (see Thornhill et al. 2012) --- JX536708 ------Parougia sp. Seep Clade OR (see Thornhill et al. 2012) --- JX536710 ------Protodorvillea kefersteini (McIntosh, 1869) DQ779670 AY838843 KF808171 DQ779759 Parougia diapason sp. nov. This study This study This study This study

References

Åkesson B, Rice SA (1992) Two new Dorvillea species (Polychaeta, Dorvilleidae) with obligate asexual reproduction. Zool Scr 21:351362 Grube AE (1855) Beschreibung neuer oder wenig bekannter Anneliden. Archiv für Naturgeschichte 21:81–136 Hilbig B, Fiege D (2001) A new species of Dorvilleidae (Annelida: Polychaeta) from a cold seep site in the northeast Pacific. P Biol Soc Wash 114:396–402 Malmgren AJ (1865) Nordiska Hafs-Annulater. Öfversigt af K. Vetenskapsakademiens förhandlingar 22:181–192 McIntosh WC (1869) On the structure of the British nemerteans, and some new British annelids. T Roy Soc Edin 25:305–433 Müller OF (1776) Zoologicae Danicae Prodromus, seu Animalium Daniae et Norvegiae indigenarum characteres, nomina et synonyma imprimis popularium. Hallageriis, Havniae [Copenhagen], 282 pp Oug E (1978) New and lesser known Dorvilleidae (Annelida, Polychaeta) from Scandinavian and northeast American waters. Sarsia 63:285–303 Taboada S, Wiklund H, Glover AG, Dahlgren TG, Cristobo J, Avila C (2013) Two new Antarctic Ophryotrocha (Annelida: Dorvilleidae) described from shallow-water whale bones. Polar Biol 36:1031–1045 Thornhill DJ, Struck TH, Ebbe B, Lee RW, Mendoza GF, Levin LA, Halanych KM (2012) Adaptive radiation in extremophilic Dorvilleidae (Annelida): diversification of a single colonizer or multiple independent lineages? Ecol Evol 2:1958–1970