Biographical Sketch s9

BIOGRAPHICAL SKETCH

NAME POSITION TITLE Sherine A. Aly MD, PhD Associate Professor eRA COMMONS USER NAME SHERINEALY EDUCATION/TRAINING DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Assiut University, Assiut, Egypt MD 1994 Medicine Assiut University, Assiut, Egypt MS 2000 Medical Microbiology Assiut University, Assiut, Egypt PhD 2004 Medical Microbiology

A. Personal Statement I am a physician/ microbiologist with over than 16 years of research experience in molecular biology during which I have been working on different topics of medical microbiology and immunology. In addition I have ten years of experience as a consultant in the Medical Microbiology Laboratory of Assiut University Hospitals Infection Control Unit. My research interest is dedicated to elucidate the genetic mechanism underlying antimicrobial resistance. Our previous work on nosocomial isolates focused on detection the genetic mechanisms responsible for extended-spectrum β-lactamase production in Klebsiella pneumoniae, methicillin resistance in Staphylococcus aureus, and vancomycin resistance in Enterococci. Recently we had a specific focus on fluoroquinolone resistance in different pathogens. In our latest work we were able to detect new mutation in Escherichia coli and to link this mutation to the multidrug resistance. This current proposal on fluoroquinolone resistant Acinetobacter baumannii aims to extend this work to uncover the mechanisms by which fluoroquinolones induce multidrug resistance. Our long term goal is to understand the relationship between the antibiotic treatment and the emergence of MDR derivatives due to mutations in bacterial pathogens and to link our research to our clinical work with the hope of bringing more specific and effective therapies to patients. I am confident that in collaboration with the outstanding Dr. Ismail research team, we will successfully develop and implement new, cost-effective diagnostic strategies aimed at detecting and reducing nosocomial infection and the worldwide spread of MDR A. baumannii. B. Positions and Honors. Positions and Employment 1995-1996 House officer at Assiut University Hospital, Assiut, Egypt 1996-2000 Demonstrator (Master Science graduate student), Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt 2000-2004 Assistant Lecturer (Ph.D. graduate student) Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt 2005-2009 Lecturer, Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt 2009-2012 Visiting Scholar, Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, USA 2012-Present Associate Professor, Department of Medical Microbiology & Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Other Experience and Professional Memberships 2010.2012 Member, American Society for Mocrobiology 2008- Present Member, Egyptian Society for Microbiology Honors 2009 The Partnership and Ownership initiative (ParOwn) 2010 Clinical Pharmacology Laboratory fellowship, Auburn University, Al, USA 2011 Research award at Auburn University Research week, Auburn, Al

C. Selected peer-reviewed publications (chronological order, 2008-2012)

1- Mona A. Hasan, Sherine A. Aly, Enas A. Daef, Ismaeil A. Seddik, and Intesar A. Hamed."Evaluation of different methods for detecting Methicillin resistant Staphylococcus aureus in Assiut University Hospital". Egyptian Journal of Medical Microbiology; 2008, Vol. 12. No 2, 187-197. 2- Enas Daef, Sherine Aly, Salwa Seif El-Din, Nahla El Sherbiny and Sherine El Gendy. Phenotypic and Genotypic detection of extended spectrum β-lactamase Klebsiella pneumoniae isolated from Intensive Care Units in Assiut University Hospital. Egyptian Journal of Medical Microbiology, 2009. Vol. 18. No 2, 29- 40. 3- Nagla Abufadan, laila Abd-El baky, Heba Rashed and Sherine Aly. “Clinico-laboratory study on children with autoimmune hepatitis in Assiut”. Arab Journal of Gastroenterology. 2011. 12; 178–183.

4- Ismail Mohamed, Amany Thabit , Sherine Aly, Essam Eldin Abdelmohsen, Salwa Seif Eldin , and Aliaa Ghandour. Prevalence of SEN Virus Infection in Multitransfused Patients in Assiut University Hospitals, Egypt. Journal of American Science, 2011;7(1) 5- Sherine A Aly, Nipattra Debavalya, Sang-Jin Suh, Omar A Oryazabal, and Dawn M Boothe. Molecular mechanisms of antimicrobial resistance in fecal Escherichia coli of healthy dogs after enrofloxacin or amoxicillin administration. Can. J. Microbiol. 2012, 58: 1–7 6- Xiaoqiang Liu, Dawn M. Boothe, Kamoltip Thungrat, and Sherine Aly. Mechanisms accounting for fluoroquinolone multidrug resistance Escherichia coli isolated from companion animals. Vet. Microbiol. 2012. 161; 159–168 D. Research Support Ongoing Research Support Intramural Assiut University Daef (PI) 04/30/2013- 03/31/2015

Genetic profile of fluoroquinolone resistant Escherichia coli and Klebsiella pneumoniae in Egypt Role: Co-PI The major goal of this project is to detect plasmids and mutations responsible to fluoroquinolone resistance in Escherichia coli and Klebsiella pneumoniae in Egypt.

Intramural Assiut University Kotb (PI) 04/30/2013- 03/31/2015

Comparative study of opioid receptors expression and localization in prostate cancer and benign prostatic hyperplasia: Implication in prostate cancer progression. Role: Co-PI The major goal of this project is to compare the change in opioid receptors gene and protein expression in human benign prostatic hyperplasia and prostate cancer.

Completed Research Support IGP-AU Boothe (PI) 01/31/2010-12/30/2012

A novel soxS mutation associated with Multidrug resistance in Fluoroquinolone resistant Escherichia coli Role: Postdoctoral Fellow The major goal of this project was to elucidate the role of a newly detected soxS mutation associated with Multidrug resistance.