Reactive Oxigen Intermediates and Antioxidant Defence in the Course of Endometriosis Disease

Abstract No: 7 REACTIVE OXIGEN INTERMEDIATES AND ANTIOXIDANT DEFENCE IN THE COURSE OF ENDOMETRIOSIS DISEASE. 1A. Kamer-Bartosinska, 2K. Szyllo, 3H. Tchorzewski, 4P. Lewkowicz, 5J. Lewy

1,2,5Depat. of Surgical Gynecology and 3Clinical Immunology, Polish Mother Health Memorial Research Institute, Poland

Endometriosis is one of the most common gynecological diseases. Pathogenesis of endometriosis is not clearly understood. The role of neutrophils and reactive oxigen intermediates (ROI) still remains not explained. ROI may be responsible for modifying immunological reactions and causeing the damage of sourrounded tissues. Capability of defending against ROI is one of the crucial points of the intensity of their effects. Due to that we decided to evaluate the ROI production and the possibilities of fighting them by the immunological system of women with endometriosis. Material and methods: The study group consisted of thirteen women at reproductive age, with clinical symptoms and confirmed diagnosis of endometriosis. Thirteen age-matched women with no disease symptoms were the control group. ROI production in women's peripheral blood was measured using the luminol enhanced chemiluminescence test (MLX Microtiter Plate Luminometr, Dynex, USA). We evaluated both non-stimulated and stimulated CL. The total antioxidant status was measured in a special testing procedure (Becton Dickinson). All the data were statistically analysed. Results: We observed higher mean values of neutrophils chemiluminescence in peripheral blood of women with endometriosis as compared to the control group. Most significant difference was noted after the fMLP stimulation. Our data also show, that there is a statistically significant decrease in total antioxidant status in the course of endometriosis as compared to women without this disease. Conclusion: We conclude that oxido-antioxidant balance is altered in women with endometriosis. Therefore reactive oxygen intermediates and total antioxidant status may play, at least partly, an important role in the pathogenesis of endometriosis disease. Acknowledgement: This study was supported by the Polish Scientific Programme (KBN 4 PO5E 108 15)