Instrumental Analysis

Chemistry 303311 Instrumental Analysis First Semester 2007/2008 Dr. Maha F. Tutunji

Textbook: 1) Principles of Instrumental Analysis Fifth Edition, Skoog Holler. Nieman (Harcourt) 2) Statistics and Chemometrics for analytical chemistry J. N. Miller

Exams and Grades First Exam : (1) Saturday 20/10/07 20% PTS Second Exam: Saturday 24/11/07 30% PTS Final Exam: (2) To be announced 50 PTS

Course Content:

Statistics and chemometrics for analytical chemistry 1. Errors in quantitative analysis Chapter 1 Types of error planning and design of experiments. (Experimental Design)

2. Calibration methods in instrumental analysis / Regression and correlation. Chapter 5 2.1 Introduction: instrumental analysis 107 2.2 Calibration graphs in instrumental analysis. 2.3 The product-moment correlation coefficient. 2.4 The line of regression of y on x. 2.5 Errors in the slope and intercept of the regression line. 2.6 Calculation of concentration and its random error. 2.7 Limits of detection. 2.8 The method of standard addition. 2.9 Weighted regression lines.

3. Principles Spectrophotometric methods of analysis

Chapter 6: An Introduction to Spectrometric Methods 116 6A General Properties of Electromagnetic Radiation 116 6B Wave Properties of Electromagnetic Radiation 117 6C Quantum-Mechanical Properties of Radiation 128 6D Quantitative Aspects of Spectrochemical 138 Measurements Chapter 7 Components of Optical Instruments 143 7A General Designs of Optical Instruments 143 7B Sources of Radiation 145 7C Wavelength Selectros 154 7D Sample Containers 166 7E Radiation Transducers 167 7H Types of Optical Instruments 181 -2-

Chapter 8 An Introduction to Optical Atomic Spectrometry 192 8A Optical Atomic Spectra 192 8B Atomization Methods 200 8C Sample Introduction Methods 201

Chapter 9 Atomic Absorption and Atomic Fluorescence 206 Spectrometry 9A Sample Atomization Techniques 206 9B Atomic Absorption Instrumentation 213 9C Interferences in Atomic Absorption Spectroscopy 217 9D Atomic Absorption Analytical Techniques 223 9E Atomic Fluoresence Spectroscopy 225

Section III Molecular spectroscopy

Chapter 13 An introduction to ultraviolet / visible molecular absorption spectrometry.

Chapter 14 Application of ultravioleted / visible molecular absorption spectrometry.

Chapter 15 Molecular luminescence spectrometry.

Chapter 16 An Introduction to Infrared spectrometry

Section V

4. Separation Methods 673

Chapter 26 An Introduction to Chromatographic Separations 674 26A A General Description of Chromatography 675 26B Migration Rates of Solutes 678 26C Zone Broadening and Column Efficiency 680 26D Optimization of Column Performance 687 26E Summary of Important Relationships for 695 Chromatography

Chapter 27 Gas Chromatography 701 27A Principles of Gas-Liquid Chromatography 702 27B Instruments for Gas-Liquid Chromatography 703 27C Gas Chromatographic Columns and Stationary 711 Phases Chapter 28 High-Performance Liquid Chromatography 725 28A Scope of HPLC 726 28B Column Efficiency in Liquid Chromatography 726 28C Instruments for Liquid Chromatography 728 Applications of HPLC