Mouse Genotyping Service Setup

Mouse Genotyping

Sample Submission

Prior to the submission of regular genotyping orders to our core facility, first-time users need to provide their validated PCR-based assay protocol(s). Our Mouse Genotyping team will test the user-validated assays with appropriate controls to ensure result replication and will further optimize the assay(s) for execution in our facility. The initial transfer of PCR-based genotyping assays is provided at no extra cost for the customer! Please note that all genotyping assays must undergo this step. For initiating the genotyping assay transfer, please contact us with your PCR protocols, including PCR reaction conditions, cycling parameters, primer sequences, and expected amplicon sizes. Please provide also evidence in support of PCR reliability in form of an Agarose gel image.

Sample Preparation Tail biopsies must be between 5 mm and 10 mm in length and placed in capped 12-strip microtubes. We recommend the use of the following tubes: - Racked Microtubes, 1.1ml 12-strips (10 Racks of 96), BioExpress, Cat# P-8706-3 - 12-Strip Microtube Caps (80 Capstrips of 12), BioExpress, Cat# P-8706-CS Samples that do not conform to the required tube format or biopsy length will not be accepted. Caution: PCR-based genotyping is sensitive, and a small amount of tissue contamination may lead to a false positive result. It is therefore imperative that scissors or razors be thoroughly washed with plenty of water and wiped-dry with ethanol between each tail snip to avoid potential cross- contamination. Clearly label each microtube on the side and fill out the Mouse Genotyping submission form during the order process. The Sample ID in the submission form must match the label on the side of the microtube. The Sample ID/label must be unique for each sample in order to avoid ambiguity. Capped microtubes may be placed in a small Ziploc plastic bag. Write "Genotyping - Store at -20°C" on the Ziploc plastic bag, to ensure storage under proper conditions once samples are delivered to the facility. For high-volume genotyping orders, we prefer to receive the microtubes in a blue 96-well rack. Submit your Mouse Genotyping Order through our web page, and include a printed copy of the order form with your sample submission. Primers You will need to submit primers for regular genotyping to the core facility with your first regular order. We will contact you for this immediately after the initial service setup. Genotyping primers can be ordered through our DNA Synthesis group and directly delivered to us if needed.

Sample Shipment

We are currently processing Mouse tail clips once a week, beginning each Monday at 2 pm. Samples received after 2 pm on Monday will be stored and processed the following Monday. Samples should be dropped off at one of our refrigerated remote drop-off locations or directly at our facility. Please have your samples inside the cooler by 10:20 AM to ensure same day delivery to the core facility, thereby avoiding sample deterioration, as samples would otherwise be picked up on the following business day. Alternatively, samples may be shipped on dry ice to the facility or delivered in person.