Disintegrin and Metalloproteinases (ADAMs) expression in Gastroesophageal Reflux Disease and in Esophageal Adenocarcinoma
Journal of Clinical and Translational Oncology
Authors: Tuuli Kauttu, Harri Mustonen, Sanna Vainionpää, Leena Krogerus, Ilkka Ilonen, Jari
Räsänen, Jarmo Salo, Pauli Puolakkainen
Corresponding author:
Pauli Puolakkainen, MD, PhD
Department of Gastroenterologic and General Surgery, Helsinki University Hospital
& Department of Surgery, Clinicum, Faculty of Medicine, University of Helsinki e-mail: [email protected], [email protected] Online Resource 1 Primary and secondary antibodies used in western blotting
Catalog Name Ig Type Producer number ADAM9 (C-15) polyclonal Santa Cruz Biotechnology, antibody Goat IgG Santa Cruz, CA sc-23290 Anti-Human ADAM10 (B-3) Total Goat R&D Systems, Minneapolis, Ectodomain antibody IgG MN AB936 ADAM12 (H-210) Santa Cruz Biotechnology, polyclonal antibody Rabbit IgG Santa Cruz, CA sc-25579 Monoclonal Anti-human ¹TACE Ectodomain Mouse R&D Systems, Minneapolis, Antibody IgG1 MN MAB9302 ADAM19 (S-20) polyclonal Santa Cruz Biotechnology, antibody Goat IgG Santa Cruz, CA sc-25988 GAPDH (6C5) monoclonal Santa Cruz Biotechnology, antibody Mouse IgG Santa Cruz, CA sc-32233 GAPDH (FL-335) Santa Cruz Biotechnology, polyclonal antibody Rabbit IgG Santa Cruz, CA sc-25778 GAPDH (V-18) polyclonal Santa Cruz Biotechnology, antibody Goat IgG Santa Cruz, CA sc-20357 Donkey-anti-goat Alexa Fluor 680 IgG (H+L) Invitrogen, Eugene, OR A21084 Goat-anti- rabbit Alexa Fluor 680 IgG (H+L) Invitrogen, Eugene, OR A21109 Goat-anti- mouse Alexa Fluor 680 IgG (H+L) Invitrogen, Eugene, OR A21058 IRDye 800CW Donkey-anti- LI-COR Bioscience, Rabbit IgG (H+L) Lincoln, NE 926-32213 IRDye 800CW Goat-anti- LI-COR Bioscience, Mouse IgG (H+L) Lincoln, NE 926-32210 IRDye 800CW Goat-anti- LI-COR Bioscience, Rabbit IgG (H+L) Lincoln, NE 926-32213
The Alexa Fuor 680 -secondary antibodies were used for ADAMs 9 and 19 and the IRDye 800CW
-secondary antibodies for ADAMs 10,12, and 17.
¹TACE = tumor necrosis factor alpha-converting enzyme = ADAM17