Human Anatomy and Physiology II Lecture (BIOL 252-171) s1

Microbiology Lab (BIOL 2230) – Fall 2013 PREP SHEETS

WK Topics Orgs Media 9 Biochemical Testing III Per Table Per Table: (Biochem 3 BAP (disk tests) 11/06 Selective and/or Differential Media Testing): 1 MAC  Mannitol Salt Agar (MSA) 4-1 1 MSA TSA Slant: 1 NAP (growth control for selective media)  S. aureus M. luteus NA Slant:  S. epidermidis Students will spot inoculate one quadrant of MAC, MSA  E. coli/E. aerogenes  S. aureus and NAP with assigned organism.  M. luteus  S. For Class Cultures :  MacConkey Agar (MAC) 4-6 epidermidis 1 TSA x 6 tables  E. coli/E. aerogenes  E. coli 7 NAS x 6 tables  E. faecalis  E. 3 NB x 6 tables  S. flexneri faecalis 5 TGB x 6 tables  S. typhimurium  S. (Students will share the 9 broth tubes between each pair of tables)  Blood Agar Plate (BAP) 5-20 typhimurium  (see demonstration plates)  S. Instructor Cultures: Disk Tests flexneri Hemolysis Demonstration  Bacitracin (A) Susceptibility 5-9  E. o 1 BAP w S. aureus  S. pyogenes aerogenes o 1 BAP w S. pyogenes Nutrient broths:  S. agalactiae o 1 BAP w S. agalactiae  S. aureus  Novobiocin Susceptibility (p. 468) o 1 BAP w S. pneumonia or bovis  S. Note: All in a candle jar!  S. aureus or S. epidermidis epidermidis  S. saprophyticus  S.  Optochin (P) Susceptibility (p. 468) saprophyticu  S. bovis s  S. pneumoniae TG broths:  S. pyogenes  S. agalactiae  S. peumonia e  S. bovis  E. faecalis

Shannon makes UNKNOWNS!!!

Media Needed: 24 nutrient broth tubes 24 thioglycollate broth tubes 24 sterile tubes