UCSF Lab Standard Operating Procedure s11

UCSF Lab Standard Operating Procedure

Chemical Process, Name or Hazard Class: 1,2-Dibromo-3-Chloropropane

Please fill out the form completely. Insure all users have access to this SOP. Refer to instructions for assistance. Department: Enter text. Date SOP was written: Enter date. This lab specific SOP has been reviewed and approved by: Principal Investigator Name: Enter text. Principal Investigator Signature Lab Manager/Supervisor: Enter text.

Type of SOP: ☐ Process ☒Hazardous Chemical ☐ Hazardous Class

Purpose

1,2-Dibromo-3-chloropropane is a regulated carcinogen. It was used as a soil fumigant up until 1977. In the laboratory, it is used as an intermediate in organic synthesis.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 96-12-8

Class: Carcinogen

Molecular Formula: C3H5Br2Cl

Form (physical state): liquid

Color: Colorless to slightly yellow

Boiling point: 196 ºC

Potential Hazards/Toxicity

It is harmful if inhaled, swallowed or absorbed through the skin. It is a severe irritant and a poison. It is a mutagen and proven to be causing male sterility.

Engineering Controls

1,2-Dibromo-3-Chloropropane 1 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Certified ducted Fume hood.

Personal Protective Equipment (PPE)

Respirator Protection

If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by EH&S. This is a regulatory requirement. (http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/)

Hand Protection Wear nitrile gloves.

NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with 1,2-Dibromo-3-chloropropane

Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf OR http://www.allsafetyproducts.biz/page/74172 OR http://www.showabestglove.com/site/default.aspx OR http://www.mapaglove.com/

Eye Protection Wear safety glasses or goggles.

Skin and Body Protection Wear a lab coat.

Hygiene Measures Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product

First Aid Procedures

If inhaled Remove to fresh air. If breathing becomes difficult, call a physician.

In case of skin contact Immediately wash skin with soap and copious amounts of water.

In case of eye contact Flush with copious amounts of water for at least 15 minutes. Assure adequate flushing by separating the eyelids with fingers. Call a physician.

If swallowed Wash out mouth with water provided person is conscious. Call a physician.

1,2-Dibromo-3-Chloropropane 2 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Special Handling and Storage Requirements

Precautions for safe handling

Do not breathe vapor. Do not get in eyes, on skin, on clothing. Avoid prolonged or repeated exposure.

Conditions for safe storage

Store in secondary container. Keep container tightly closed, and labeled “Danger: Regulated Carcinogen”. OK to store in flammable cabinet.

Spill and Accident Procedure Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only)

Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206- 8522 (SFGH only) for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800- 222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415- 476-1300 during work hours, or 9-911 during non-working hours and weekends.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.

Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

1,2-Dibromo-3-Chloropropane 3 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/) Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885- 7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday 7:30 a.m. - 4:00 p.m. (except Holidays).

Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).

Decontamination/Waste Disposal Procedure

Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.

Safety Data Sheet (SDS) Location

Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html

Protocol/Procedure

Quantities covered by this SOP: ______(g , ml) to ______(g, ml)

Temperature range covered by this SOP: __ °C – __ °C

General Overview and Purpose: Enter the experimental purpose

Procedure: Enter experimental procedure. You can copy procedure from your lab notebook or from literature.

Any deviation from this SOP requires approval from the Principal Investigator.

1,2-Dibromo-3-Chloropropane 4 Date: Click here to enter a date. Modified from SOP templates developed by UCLA EH&S (http://www.sop.ehs.ucla.edu/)