Caps/Sslp Marker Description Form

D:\Docs\2018-01-08\028cab56ebdaf31dd5d7d76985416214.doc 15 March 2007 SLK1_043160 CAPS/SSLP MARKER DESCRIPTION FORM

Franks Lab

Date: Sept 17, 07

NAME of CAPS/SSLP MARKER – SLK2_CS842569 (in gene At5G62090)

Purpose - To identify het and homozygous CS842569 (SAIL) line inserts in SLK2 gene. oligo1: slk2-CS842569f 1l oligo2: slk2-CS842569r 1l oligo3: pCSA110 LB3 0.3 l annealing temp: 55 deg extension time at 72 deg.: 1 min.

MgCl2 conc.: 1.5 mM other special conditions:

Expected sizes of amplicons

Wild type band = 1.0 kb mutant band = 0.7 kb and 0.48 kb

Note: If do 2 separate reactions, the mutant reaction can use either F+LBb1 (700 bp) or R+LBb1 (480 bp)..