Responses of Bacterial and Fungal Communities to an Elevation Gradient

Responses of bacterial and fungal communities to an elevation gradient

in a subtropical montane forest of China

Han Meng, Ke Li, Ming Nie, Jia-Rong Wan, Zhe-Xue Quan, Chang-Ming Fang,

Jia-Kuan Chen, Ji-Dong Gu and Bo Li

M Nie, JR Wan, JK Chen, B Li

Centre for Watershed Ecology, Institute of Life Science and Key Laboratory of Poyang Lake Environment and Resource Utilization, Nanchang University, Nanchang 330031, P.R. China

H Meng, K Li, M Nie, ZX Quan, CM Fang, JK Chen, B Li

School of Life Sciences, Fudan University, Shanghai 200433, P.R. China

School of Biological Sciences, The University of Hong Kong, Pofulam Road, Hong Kong SAR, P.R. China

Table S1 ∫-libshuff analysis for bacteria from different elevations of forest soils Comparison dCXY Score HE-LE 0.000206 LE-HE 0.001747 HE-ME 0.000441 ME-HE 0.000486 LE-ME 0.000902 ME-LE 0.000231 LE: low elevation; ME: medium elevation; HE: high elevation. Table S2 Comparison of soil bacterial diversity from high elevation through pyrosequencing and clone library methods

Ord Phylum Class Family Genus High Elevation er Clone Pyrosequencing % % library Aci dob act Acidobacteriacea Acidobacteria Acidobacteria Acidobacterium 56 4.09 0 0 eri e ale s 1 Candidatus_Kori 66 4.82 10 2. bacter 5 Unclassified 52 3.8 0 0 Candidatus_Solib 13 0.95 0 0 acter 1. Edaphobacte 21 1.53 1 3 2 Unclassified Unclassified 88 6.43 22 7. 5 Act ino my Catenulisporinea Actinobacteria Actinobacteria Actinospica 1 0.07 0 0 cet e ale s Corynebacterine Mycobacterium 20 1.46 0 0 ae Unclassified 2 0.15 0 0 Corynebacterium 2 0.15 0 0 Gordonia 1 0.07 0 0 Micromonospori Unclassified 1 0.07 0 0 neae Propionibacterin Propionibacteriu 3 0.22 0 0 eae m Streptomycineae Kitasatospora 1 0.07 0 0 Frankineae Acidothermus 51 3.73 0 0 Unclassified 3 0.22 0 0 Soli rub rob Conexibacterace 1. Conexibacter 10 0.73 1 act ae 3 era les Solirubrobactera Solirubrobacter 18 1.31 0 0 ceae Aci dim icro Acidimicrobineae Unclassified 83 6.06 0 0 bial es 1. Unclassified 23 1.68 1 3 Sph ing oba Chitinophagacea Bacteroidetes Sphingobacteria Segetibacter 2 0.15 0 0 cter e iale s Unclassified 14 1.02 0 0 Chitinophaga 2 0.15 0 0 Sphingobacteriac Mucilaginibacter 3 0.22 0 0 eae Unclassified 1 0.07 0 0 Unc lass 3 0.22 0 0 ifie d Unclassified 2 0.15 0 0 1. Candidate_division_OP10 Unclassified 1 0.07 1 3 Candidate_division_TM6 Unclassified 1 0.07 0 0 Candidate_division_TM Unclassified 8 0.58 0 0 7 Chloroflexi Unclassified 33 2.41 0 0 Bac Firmicutes Bacilli illa Paenibacillaceae Paenibacillus 1 0.07 0 0 les Unclassified 2 0.15 0 0 Bacillaceae Bacillus 1 0.07 0 0 Unclassified 1 0.07 0 0 Lac tob Carnobacteriace acil Dolosigranulum 3 0.22 0 0 ae lale s Streptococcaceae Streptococcus 1 0.07 0 0 Clo stri Clostridia Unclassified 1 0.07 0 0 dial es Veillonellaceae Unclassified 1 0.07 0 0 Ge mm ati Gemmatimonada Gemmatimonadetes Gemmatimonadetes mo Unclassified 13 0.95 0 0 ceae nad ale s Pla nct om Planctomycetace Planctomycetes Planctomycetacia Planctomyces 7 0.51 0 0 yce ae tale s Schlesneria 1 0.07 0 0 1. Singulisphaera 7 0.51 1 3 6. Unclassified 22 1.61 5 3 Rhi zob 1. Proteobacteria Alphaproteobacteria Beijerinckiaceae Methylocella 2 0.15 1 iale 3 s Chelatococcus 3 0.22 0 0 Beijerinckia 2 0.15 0 0 Unclassified 1 0.07 0 0 Bradyrhizobiace Bradyrhizobium 18 1.31 0 0 ae Nitrobacter 1 0.07 0 0 1. Rhodoblastus 1 0.07 1 3 Blastobacter 1 0.07 0 0 Afipia 25 1.83 0 0 Unclassified 2 0.15 0 0 Hyphomicrobiac Pedomicrobium 1 0.07 0 0 eae Hyphomicrobium 1 0.07 0 0 Devosia 1 0.07 0 0 Unclassified 1 0.07 0 0 Methylocystacea Unclassified 9 0.66 0 0 e Xanthobacterace 7. Unclassified 51 3.73 6 ae 5 Unclassified 47 3.43 0 0 Rh odo Acetobacteriacea spir Acidocella 1 0.07 0 0 e illa les Acidisphaera 1 0.07 0 0 Unclassified 22 1.61 0 0 Unclassified 134 9.79 4 5 Unc 1 lass 19 1.39 10 2. ifie 5 d Bur kho Burkholderiacea 1. Betaproteobacteria lde Burkholderia 12 0.88 1 e 3 rial es Alcaligenaceae Unclassified 1 0.07 0 0 Rh odo 1. cyc Rhodocyclaceae Sterolibacterium 0 0 1 3 lale s 1. Unclassified 4 0.29 1 3 Nitr oso mo Nitrosomonadace Unclassified 1 0.07 0 0 nad ae ale s My xoc Deltaproteobacteria occ Cystobacterineae Unclassified 3 0.22 0 0 ale s 1. Kofleriaceae Haliangium 0 0 1 3 Unclassified 11 0.8 0 0 Unc 26 1.9 3 3. lass 8 ifie d Leg Gammaproteobacteri ion Coxiellaceae Aquicella 5 0.37 0 0 a ella les Unclassified 1 0.07 0 0 Legionellaceae Legionella 2 0.15 0 0 Unclassified 1 0.07 0 0 Chr om Granulosicoccac 1. Granulosicoccus 0 0 1 atia eae 3 les Xa nth om Xanthomonadace Dyella 3 0.22 0 0 ona ae dal es Rhodanobacter 1 0.07 0 0 Dokdonella 1 0.07 0 0 Unclassified 4 0.29 0 0 1. Sinobacteraceae Steroidobacter 0 0 1 3 Unclassified 41 2.99 0 0 Unc lass 25 1.83 0 0 ifie d Unclassified 15 1.1 0 0 Gemmatimonadetes Gemmatimonadetes Ge Gemmatimonada Gemmatimonas 7 0.51 0 0 mm ceae ati mo nad ale s Unclassified 6 0.44 0 0 Opi Verrucomicrobia Opitutae tuta Opitutaceae Opitutus 6 0.44 0 0 les Unclassified 1 0.07 0 0 Candidatus_Xiph 2. Spartobacteria 11 0.8 2 inematobacter 5 Chthoniobacter 3 0.22 0 0 Unclassified 30 2.19 0 0 2. Unclassified 23 1.68 2 5 Cyanobacteria Unclassified 14 1.02 0 0 3. Unclassified 121 8.84 3 8 1 Total 1369 100 80 0 0