Laboratory Standard Operating Procedure

Laboratory Standard Operating Procedure (For the Use of Hazardous Materials or Equipment)

Name of Procedure: High Performance Liquid Chromatography - HPLC______

Prepared by: ______Revision Date: ______

LOCATION- This procedure may be performed in the following location(s):

Building :

Room #:

HAZARDS- The following materials and equipment associated with this procedure present exposure or physical health hazards. The following hazards require safety precautions:

ACETONITRILE (CAS # 75-05-8)- May be fatal if swallowed, inhaled or absorbed through the skin. Affects cardiovascular system, central nervous system, liver and kidneys. Flammable liquid and vapor. May cause irritation to skin, eyes, and respiratory tract. Review MSDS prior to working with Acetonitrile.

METHANOL (CAS # 67-56-1) – Vapor harmful. May be fatal or cause blindness if swallowed. Harmful if inhaled or absorbed through the skin. Cannot be made non-poisonous. Flammable liquid and vapor. Causes irritation to skin, eyes, and respiratory tract. Affects central nervous system and liver. Review MSDS prior to working with Methanol.

ENGINEERING CONTROLS: Prior to performing this procedure, the following safety equipment must be accessible and ready for use: (ex. Chemical fume hood, biological safety cabinet, laminar flow hood)

Containers should be capped when not in use.

PROTECTIVE EQUIPMENT- Prior to performing this procedure, the following personal protective equipment (PPE) must be obtained and ready for use: (ex. Acid resistant gloves, safety eyewear, lab coat, chemical splash apron)

Nitrile gloves are suitable for handling containers and cleaning up small spills of Acetontrile and Methanol. If accidental contact occurs, remove and discard contaminated gloves immediately. Break though may occur after prolonged exposure. Safety glasses and lab coat must be worn when performing all tasks associated preparing and running HPLC analysis. Wear chemical splash goggles when there is a splash hazard (when pouring chemicals).

WASTE DISPOSAL- This procedure will result in the following regulated waste, which must be disposed of in compliance with the REHS Hazardous Waste Policy: Acetonitrile and Methanol waste must be collected and disposed of via REHS.

ACCIDENTAL SPILL- In the event of a spill of hazardous materials, follow these procedures:

Small Spill Large Spill ASSESS spill size, chemical type and available ATTEND to injured persons, seek appropriate cleanup equipment. medical attention. EVACUATE the immediate area. EVACUATE area/building. WARN other personnel. EXTINGUISH ignition and heat sources. WEAR appropriate PPE. PREVENT spill from spreading. CLEAN UP spill using appropriate spill kit if trained CONTACT REHS for assistance. After hours call to do so. Campus Police. CONTAIN spilled material in a sturdy plastic bag or FILE a formal incident report sealed drum. Apply a hazardous waste label and contact REHS for disposal.

PRIOR APPROVAL- This procedure is considered hazardous enough to warrant prior approval from the PI or department safety coordinator: YES/NO