Table 1 Primers Used for Fine Mapping of the Br-Or Locus

Table 1 Primers used for fine mapping of the Br-or locus

Primer size Motif Primer Primer sequences 5′-3′ Character names (bp) Product type Br-SSR1 Forward CCATCAACAAACTCAACCCTG 150 (TC)11 SSR Co-dominant Reverse CTCTGTTCATAAATACGCCTC Br-SSR2 Forward GGTCCGCACAACATATCCTT 208 (TA)6 SSR Co-dominant Reverse TTGTAGCGGTGTCTAAGCAG Br-SSR3 Forward ACGCTCACCGTGCTTCCTTG 182 (CTC)8 SSR Co-dominant Reverse TTCACTTTGCCTTTGTTTCT Br-SSR4 Forward ATCACGTCTACTGGTGCTATG 122 (AC)6 SSR Co-dominant Reverse CCACTCCCTGTATTCCTTATC Br-SSR5 Forward GCCAAAACAAATCTGCTTGA 189 (CT)5 SSR Co-dominant Reverse GTTTGGTCTGTTTCGGCACT Br-SSR6 Forward AATCTAAACCTGGACCGCAAC 203 (TG)10 SSR Co-dominant Reverse GTGGGATTTCTGGAGCTTTCA Br-SSR7 Forward TTTCGTCTATTCTTGCTGGAT 193 (TG)5 SSR Co-dominant Reverse AAGACACCGTGAGTATCTTCT Br-SSR8 Forward TGTGAATGTGCGAATTTTGA 114 (CT)9 SSR Co-dominant Reverse TCCTCCTACGCTCTTCATCT Br-SSR9 Forward TTACGCTCAAGTTCCTGAAT 261 (CT)5 SSR Co-dominant Reverse GCGATGTACGCTAACGAGAT Br-SSR10 Forward TTGGTTTCACTGATGTTTCT 168 (TA)11 SSR Co-dominant Reverse CCATATTTGGTTGATTGTTC Br-SSR11 Forward AGCTCCTGTATGGAACTCAA 123 (TA)7 SSR Co-dominant Reverse TTCCTTTCTTCTGACAAATC Br-SSR12 Forward AATCAACGAAAACGAGGAAT 200 (GA)8 SSR Co-dominant Reverse GCTCAGATAGAGAAAGGGGG Br-SSR13 Forward TGTAACTTAAAGAAAACCAAAA 175 (AT)7 SSR Co-dominant Reverse CATCACATACTAATCAACCAGA Br-SSR14 Forward GTAATGGAGAGTGATGATGA 234 (TA)10 SSR Co-dominant Reverse TTTGGAACTAAATTGTTTGT Br-SSR15 Forward ATTTGGATCGTTGATTGAAT 212 (AT)11 SSR Co-dominant Reverse ACTTTTTGCTTGTTTGTTTT syau19 Forward CCGAAGCCCAGATTAGGAG 322 (AG)16 SSR Co-dominant Reverse GCCCATGCCTACGAATGATAT Br-indel1 Forward TGAAATGACCCGTCTGTTGA 280 -- SSR Co-dominant Reverse TTGTCTTGCGAAGAGGATGT Br-indel2 Forward TCAGATGAAGAGCGTAGGAG 152 -- InDel Co-dominant Reverse AAAAGAGGGAGACAAGATGC Br-indel3 Forward TTCTTCTTCCTCACGCACTC 120 -- InDel Co-dominant Reverse GCTCGCCTTCTAAACTGAAT Br-pds1 Forward TCTCTAGAAATGCGTCTGACC 423 -- InDel Co-dominant Reverse TTGGACATCCAAGAGTACACG Br-crtiso Forward GAATACGGGACATGGAATCAG 334 -- InDel Co-dominant Reverse CACCTAATCGGCAAATCGAA Br-scar1 Forward GGCACTGAGGATTGGGTTC 591 -- SCAR Dominant Reverse GGCACTGAGGTACATGAAC Table 2 Gene annotation of the three ORFs in 16.7-kb DNA fragment on linkage group 9 Homologous Arabidopsis Gene in Number Gene annotation gene and E value B.rapa Genome ORF1 AT1G06820, 0 Bra031539 Carotenoid isomerase RNA polymerase Rpb7 N-terminal ORF2 AT1G06790, 3.00E-75 Bra031538 domain-containing protein ORF3 AT1G06780, 0 Bra031537 Galacturonosyltransferase 6