Table 2S. Antibodies Used in Western Blot

Table 1S. Pre-designed TaqMan gene Expression Assay kits (Life Technologies) used for quantitative real-time RT-PCR.

Target gene ID

Ftl ferritin, light polypeptide Rn00391197_ml

Slc40a1 solute carrier family 40 (iron-regulated Rn00391197_ml transporter), member 1

ferroportin Nos2 (iNOS) nitric oxide synthase 2, inducible Rn00561646_m1

Nos3 (eNOS) nitric oxide synthase 3, endothelial cell Rn02132634_s1

Tfrc (Tfr1) transferin receptor protein 1 Rn01474701_m1

Tfr2 transferrin receptor protein 2 Rn01481694_ml

Hamp hepcidin antimicrobial peptid Rn00584987_ml

Abcc2 (Mrp2) ATP – binding cassette, subfamily C Rn00563231_ml (CFTR/MRP), member 2

multidrug resistance-associated protein 2

Slc10a1 (Ntcp) solute carrier family 10 Rn00566894_ml

(sodium/bile acid cotransporter), member 1 Abcb11(Bsep) ATP - binding cassette, subfamily B Rn00582179_ml

(MDR/TAP), member 1

(bile Salt Export Pump) Slco1a2 Solute carrier organic anion transporter family, Rn00756233_m1 (Oatp1a4) member 1A2

Tnf (Tnf Tumor necrosis factor alpha Rn99999_ml

Il-6 (IL-6) Interleukin 6 Rn99999011_ml

NrOb2 (Shp) nuclear receptor subfamily 0, group B, Rn00589173_ml member 2 2

Slc11a2 (Dmt1) solute carrier family 11 (proton-coupled Rn 00565927_m1 divalent metal ion transporter), member 2 Cyp7a1 cytochrome P450, family 7, subfamily a, Rn00564065_ml polypeptide 1 Gapdh glyceraldehyde-3-phosphate dehydrogenase Rn01775763_g1

Table 2S. Antibodies used in Western blot. 3

Anti-mouse and anti-rabbit secondary antibodies were obtained from GE Healthcare Life

Sciences: ECL Mouse IgG, HRP-linked F(ab')2 fragment (Cat.No. NA931) and ECL Rabbit

IgG, HRP-linked F(ab')2 fragment (Cat.No. NA934). Incubation period for each antibody was

Primary antibody Secondary antibody Protein Source Cat.No. Dilution Dilution beta-actin Sigma A5316 1:5000 1:10000 iNOS BD 610329 1:100 1:2000

Biosciences eNOS BD 610298 1:1500 1:2500

Biosciences ferroportin-1 Abcam Ab85370 1:1000 1:2000 hepcidin Abcam Ab30760 1:1000 1:2000 Transferrin Abcam Ab82411 1:1000 1:2000