OIE RL for White Spot Disease Dr. Jie Huang China

OIE Reference Laboratory Reports Activities in 2012

Name of disease (or topic) for which you White spot disease are a designated OIE Reference Laboratory: Address of laboratory Maricultural Organism Diseases Control & Molecular Pathology Laboratory Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences #106 Nanjing Road, Qingdao, SD 266071 P.R. China Tel.: Phone: +86 532 85823062 Mobile: +86 13805421513 Fax: +86 532 85811514 e-mail address: [email protected] [email protected] website: www.ysfri.ac.cn Name (including Title) of Head of Dr. Jie Huang, Senior Researcher Laboratory (Responsible Official): Name (including Title and Position) of OIE Dr. Jie Huang, Senior Researcher Reference Expert:

Date of submission to the OIE Jan, 25, 2013

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at: http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/

Each ToR (blue italicised text) has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site: (http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):

Annual reports of OIE Reference Centres, 2012 1 OIE RL for « White Spot Disease » – « Dr. Jie Huang » – « China »

ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards

Test recommended by the OIE Total number of test performed last year

Indirect diagnostic tests Nationally Internationally

bioassay 34

Direct diagnostic tests Nationally Internationally

PCR 332 7

qPCR 30

histopathology 4

LAMP 18

ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?

Yes No

3. Did your laboratory supply standard reference reagents to OIE Member Countries?

Yes No

Amount Amount Type of Related Name of recipient OIE Produced/ supplied supplied reagent diagnostic Member Countries and stored nationally internationally available test of institutions (ml, mg) (ml, mg) WSSV-infected infection stored 30g (positive) 0 Ocean University of tissues experiment China WSSV-infected Positive stored 20g (positive) 0 Guangxi and Fisheries tissues control Technology Promotion Station, China WSSV-infected infection stored 20g (positive) 0 YSFRI, China tissues experiment WSSV-infected infection stored 20g (positive) 0 Mariculture Institute of tissues experiment Shandong Province, China WSSV-infected infection stored 50g (positive) 0 Ocean University of China tissues experiment WSSV-infected infection stored 30g (positive) 0 Ocean University of China tissues experiment High sensitive WSSV produced 0 8 kits The Suez Canal University, detection kits detection Egypt for detection of

2 Annual reports of OIE Reference Centres, 2012 OIE RL for « White Spot Disease » – « Dr. Jie Huang » – « China »

Amount Amount Type of Related Name of recipient OIE Produced/ supplied supplied reagent diagnostic Member Countries and stored nationally internationally available test of institutions (ml, mg) (ml, mg) white spot syndrome virus (WSSV) High sensitive WSSV produced 260 kits 0 shrimp farms, hatcheries, detection kits detection aquaculture disease for detection of control stations, some white spot research laboratories in syndrome virus the coastal provinces of (WSSV) China

4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents? Yes No

5. Did your laboratory produce vaccines? Yes No

6. Did your laboratory supply vaccines to OIE Member Countries?

Yes No

ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

Yes No

8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

Yes No

Name of the new test or diagnostic Description and References (Publication, website, etc.) method or vaccine developed Dot Blot Hybridization Method with DNA Huang J, et al. (2012). Diagnostic Protocols for White Spot Probe Disease of Shrimp, Part 1: Dot Blot Hybridization Method with DNA Probe. National Standard of People’s Republic of China, Standards Press of China, Beijing, GB/T 28630.1- 2012. Nested PCR Method Huang J, et al. (2012). Diagnostic Protocols for White Spot Disease of Shrimp, Part 2: Nested PCR Method. National Standard of People’s Republic of China, Standards Press of China, Beijing, GB/T 28630.5-2012. In situ Hybridization Method Huang J, et al. (2012). Diagnostic Protocols for White Spot Disease of Shrimp, Part 3: In situ Hybridization Method. National Standard of People’s Republic of China, Standards Press of China, Beijing, GB/T 28630.5-2012.

Annual reports of OIE Reference Laboratories, 2012 3 OIE RL for « White Spot Disease » – « Dr. Jie Huang » – « China »

Histopathology Method Huang J, et al. (2012). Diagnostic Protocols for White Spot Disease of Shrimp, Part 4: Histopathology Method. National Standard of People’s Republic of China, Standards Press of China, Beijing, GB/T 28630.5-2012. T-E Staining diagnostic method for WSD 4.2.3.1.1 T-E staining, Chapter 2.2.6. White spot disease, Manual of Diagnostic Tests for Aquatic Animals, 2012, OIE

ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes No

Name of OIE Date No. samples received for No. samples received for Member Country (dd/mm) provision of diagnostic support provision of confirmatory seeking assistance (i.e. from surveillance campaign) diagnoses

Madagascar 15/08 14

10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes No

Name of the OIE Member Country How the advice was Purpose receiving a technical consultancy provided Asia-Pacific Countries NACA Assia-Pacific Regional Expert Presentation on the research Consultation on Emerging Shrimp Disease progresses and control EMS/AHPNS experiences from China The Suez Canal University, Egypt the Use of the high sensitive detection kits remote by Emails for detection of white spot syndrome virus (WSSV) Ramy, a graduate student from Tunisia the bioassay technology for WSSV infection in loco

Shrimp farms in China diagnosis of shrimp diseases in loco Shrimp farms in China diagnosis of shrimp diseases remote by the remote diagnosis network The Veterinary Bureau, Ministry of collection of comments for OIE standards for in loco Agriculture, China aquatic animals The Fisheries Bureau, Ministry of comments for the situations of diseases of remote by Emails Agriculture, China aquatic animals

ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

Yes No

ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12. Did your Laboratory collect epizootiological data relevant to international disease control?

Yes No

13. Did your laboratory disseminate epizootiological data that had been processed and analysed?

Yes No

14. What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals:...... 9 b) International conferences:...... 5 c) National conferences:...... 6 d) Other:...... 8

Articles published in peer reviewed journals: Zhao P, Huang J, Wang X-H, Song X-L, Yang C-H, Zhang X-G, Wang G-C. The application of bioflocs technology in high-intensive, zero exchange farming systems of Marsupenaeus japonicus. Aquaculture, 2012, 354-355: 97--106. Chou Z-G, Liu Q-H, Huang J. Efficiency of two fragments of VP28 against white spot syndrome virus in Litopenaeus vannamei. Aquaculture, 2012, 338-341: 2--12. Li Q, Liu Q-H, Huang J. VP90 of white spot syndrome virus interacts with VP26 and VP28. Acta Virologica, 2012,56: 57--62. Zhang X, Ji X, Cui Z, Yang B, Huang J.Detection of sequence-specific gene by multi-channel electrochemical DNA chips. Bull. Korean Chem. Soc, 2012, 33(1): 69--75. Deng Y-N, Zhao P, Sun Y-Z, Yang C-H, Huang J. Conditions for bio-floc formation in closed culture system of Litopenaeus van-namei and its effects. (Chinese J.) Progress in Fishery Sciences, 2012, 33(2): 69--75. Sun Y-Z, Zhao P, Wang Y-H, Zhao C-M, Deng Y-N, Yang C-H, Huang J. Effect of brown sugar and Bacillus addition on water quality control for indoor intensive culture of Marsupenaeus japonicus. (Chinese J.) Progress in Fishery Sciences, 2012, 33(3): 70--76. Zhang J-Y, Liu Q-H, Huang J. Protection efficacy of vp28 DNA vaccine and immune responses in Litopenaeus vannamei against white spot syndrome virus. (Chinese J.) Progress in Fishery Sciences, 2012, 33(3): 77--82. Liu J, Song X-L, Liu L, Chai P-C. Effects of digestive tract probiotics on immune enzyme activity and anti-WSSV ability of Litopenaeus vannamei. Journal of Fisheries of China, 2012,36(3): 444-450. Sun Y, Liu F, Song X-L, Mai K-S, Li Y-H, Huang J. Effects of adding probiotics in the feed on non-specific immune gene expression and disease resistance of Litopenaeus vannamei. Oceanologia et Limnologia Sinica, 2012, 43(4): 845-851.

International conferences: Huang J. China Experiences from EMS/AHPNS. NACA Assia-Pacific Regional Expert Consultation on Emerging Shrimp Disease EMS/AHPNS, Aug 9-10, 2012. Bangkok, Thailand. Huang J. Aquatic Animal Health Standards Commission -- where to from here. the OIE Regional Training Workshop for National Focal Points on Aquatic Animals, Oct 10-12, 2012, Bangkok, Thailand. Huang J. Outcomes and Recommendations from OIE General Session and the Aquatic Animal Health Standards Commission, NACA 11th Adversary Group Meeting on Aquatic Animal Health, Nov 21-23, 2012, Bangkok, Thailand.

Huang J. New OIE Disease List, NACA 11th Adversary Group Meeting on Aquatic Animal Health, Nov 21-23, 2012, Bangkok, Thailand. Huang J. Research Progression on EMS/AHPNS. The 8th Conference for World Chinese Crustacean Aquaculture, Nov 17-18, 2012, Ningbo, China

National conferences: Huang J. The International standards for aquatic animal health. The 3rd Veterinary Conference of China, Oct 28-30, 2012, Suzhou, China. Huang J. The diagnosis and prevention of shrimp diseases. The Training Workshop of Diagnostic Laboratory for Aquatic Animal Health, Jun. 14, 2012, Huheiot, China. Huang J. The International standards for aquatic animal health. The Workshop for Animal Health and Medician Control, Nov. 29, Beijing, China. Liu Q-H, Sun F, Ma F-F. The expression of VP136c of WSSV and its function analysis. The 2012 Annual Conference of Chinese Society of Aquaculture, Nov. 6-9, 2012, Zhengzhou, China. Liu Q-H, Sun F, Ma F-F. The interaction between HSP70 of Litopenaeus vannamei and white spot syndrome virus. The 2012 Annual Conference of Chinese Society of Aquaculture, Nov. 6-9, 2012, Zhengzhou, China. Huang J, Shi C-Y, Wang X-H, Liang Y. The surveillence and pathogen analysis for marine fish and crustaceans inNorthern China. The Annual Workshop for the Aquatic Animal Disease Control in Chinese Academy of Fishery Sciences, March 14-16, Wuhan, Hubei.

Standardisation and validation of diagnostic tests: Huang J, Yang B, Chen A-P, Song X-L, Shi C-Y, Yang C-H, Wei Q. Diagnostic protocols for White Spot Disease of Penaeid Shrimp ---- Part 1: Dot blot hybridization method with DNA probe. 2012. National Standards of People’s Republic of China, China standard press, GB/T28630.1-2012 Huang J, Yang B, Chen A-P, Song X-L, Shi C-Y, Yang C-H, Wei Q. Diagnostic protocols for White Spot Disease of Penaeid Shrimp ---- Part 2: Nested PCR method. 2012. National Standards of People’s Republic of China, China standard press, GB/T28630.2-2012. Huang J, Yang B, Chen A-P, Song X-L, Shi C-Y, Yang C-H, Wei Q. Diagnostic protocols for White Spot Disease of Penaeid Shrimp ---- Part 3: In situ hybridization method.2012.National Standards of People’s Republic of China ,China standard press, GB/T28630.3-2012. Huang J, Yang B, Chen A-P, Song X-L, Shi C-Y, Yang C-H, Wei Q. Diagnostic protocols for White Spot Disease of Penaeid Shrimp ---- Part 4: Histopathology method.2012 National Standards of People’s Republic of China, China standard press, GB/T28630.4-2012. Huang J, Yang B, Chen A-P, Song X-L, Shi C-Y, Yang C-H, Wei Q. Diagnostic protocols for White Spot Disease of Penaeid Shrimp ---- Part 5: T-E staining method with fresh tissue. 2012.National Standards of People’s Republic of China, China standard press, GB/T28630.5-2012.

Granted national invention patents: Huang J, Jin X-Y, Yang B, Zhang Q-L, Yan L, Wang X-H, Zhang B-C, Song X-L, Shi C-Y, Liu L. the biochips and its detection method for multi-pathogens of Penaeid shrimp. ZL 2010 1 0243399.3, Authorized announcement, 18 July, 2012. Song X-L, Liu L, Huang J, Yang B. The bacteria stain of Vibrio alginolyticus and its application (ZL 2010 1 0254912.3). Authorized announcement. 18 April, 2012. Song X-L, Huang J, Yang B, Liu L., The bacteria stain of Bacillus firmus and its application (ZL 2010 1 0254913.8). Authorized announcement. 3 October,2012.

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

Yes No

a) Technical visits:...... 0 b) Seminars:...... 308 c) Hands-on training courses:...... 0 d) Internships (>1 month):...... 0

Type of technical training Country of origin of the expert(s) No. participants from the provided (a, b, c or d) provided with training corresponding country

b China 85 b China 23 b China 200

ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16. Does your laboratory have a Quality Management System certified according to an International Standard?

Yes No

Quality management system adopted

We are practicing the Quality Management System of CNAS following the ISO17025 and preparing for the certification in next year.

17. Is your laboratory accredited by an international accreditation body?

Yes No

18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

Yes No

ToR: To organise and participate in scientific meetings on behalf of the OIE

19. Did your laboratory organise scientific meetings on behalf of the OIE?

Yes No

20. Did your laboratory participate in scientific meetings on behalf of the OIE?

Yes No

Title of event Date Location Role (speaker, Title of the work presented (mm/yy) presenting poster, short communications) the OIE Regional 10/12 Bangkok, speaker Aquatic Animal Health Training Workshop for Thailand Standards Commission -- National Focal Points where to from here

on Aquatic Animals

NACA 11th Adversary 11/12 Bangkok, speaker 1. Outcomes and Group Meeting on Thailand Recommendations from OIE Aquatic Animal Health General Session and the Aquatic Animal Health Standards Commission 2. New OIE Disease List NACA Assia-Pacific 8/12 Bangkok, speaker China Experiences from Regional Expert Thailand EMS/AHPNS Consultation on Emerging Shrimp Disease EMS/AHPNS The 3rd Veterinary 10/12 Suzhou, China speaker The International standards Conference of China for aquatic animal health

ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

Yes No

22. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

Yes No

Purpose of the proficiency tests: (validation Role of your Reference Participating OIE of a diagnostic protocol: specify the test; Laboratory (organiser/ No. Ref. Labs/ quality control of vaccines: specify the participant) participants organising OIE vaccine type, etc.) Ref. Lab.

Regional Proficiency Testing Programme for Aquatic Participant 1 DAFF, CSIRO AAHL, Animal Disease Laboratories in Asia-Pacific ANQAP and NACA

23. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes No

ToR: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

Yes No

ToR: To place expert consultants at the disposal of the OIE

25. Did your laboratory place expert consultants at the disposal of the OIE?

Yes No

Kind of consultancy Location Subject (facultative )

OIE expert for WSD remote by Emails Comments and assessment on the application for the certification of Insulated isothermal PCR with fluorescent probe test as validated fit for specific purposes OIE expert for WSD remote by Emails Opinion on the registeration situation of a Diagnostic kit registered for WSD for next five years Vice-President of Aquatic Animal Health Paris October meeting of AAHSC for the Standards Commission revision of Aquatic Code and Aquatic Manual Vice-President of Aquatic Animal Health Bangkok, Thailand Informing the outcomes of OIE Standards Commission AAHSC for the NACA 11th Adversary Group Meeting on Aquatic Animal Health Vice-President of Aquatic Animal Health Bangkok, Thailand Informing the AAHSC working plan Standards Commission for the Asian-Pacific Regional OIE focal points for Aquatic Animal Health Vice-President of Aquatic Animal Health Bangkok, Thailand The Emergency Consultation for Standards Commission EMS/AHPNS Member of Aquatic Animal Health Paris March meeting of AAHSC for the Standards Commission revision of Aquatic Code and Aquatic Manual

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Annual reports of OIE Reference Laboratories, 2012 9