Jim Manos B.Sc. (Hons Class 1) Ph.D MASM

Curriculum Vitae

CONTACT INFORMATION

Address: Department of Infectious Diseases and Immunology, Blackburn Building D06, Sydney Medical School, University of Sydney, 2006

Phone: 02 9351 8942 Fax: 02 9351 5319

Email: [email protected]

Citizenship: Australian

EDUCATION

2000 Ph.D. (Microbiology) University of New South Wales, Sydney, NSW, Australia.

1992 HONOURS (Class 1) Microbiology University of NSW, Sydney, NSW, Australia.

1991 B.Sc. University of Sydney, NSW, Australia Majors: Biochemistry and Microbiology.

POSITION 2012-present Senior Lecturer in Infectious Diseases : Department of Infectious Diseases, Sydney Medical School, University of Sydney.

2007-2011 Lecturer in Infectious Diseases : Department of Infectious Diseases, Sydney Medical, School University of Sydney.

2004-2007 Research Fellow: Department of Infectious Diseases, School of Medicine, University of Sydney.

2001-2003 Research Associate: University of Maryland Biotechnology Institute, Center of Marine Biotechnology.

1998-2001 Postdoctoral Fellow: University of Maryland Biotechnology Institute, Center of Marine Biotechnology. CV - Dr Jim Manos

PEER REVIEWED PUBLICATIONS: 1. Modulation of gene expression by Pseudomonas aeruginosa during chronic infection in the adult CF lung . Harmer C, Alnassafi K, Hu H, Elkins M, Bye P, Rose B, Cordwell S, Triccas JA, Harbour C, Manos J. Microbiology. 2013 159: 2354-2363 PMID: 24014664. 2. The secretome of transmissible Pseudomonas aeruginosa AES-1R grown in a cystic fibrosis lung-like environment. Scott NE, Hare NJ, White MY, Manos J, Cordwell SJ. J. Proteome Res. 2013 Aug 30 [Epub ahead of print] 10.1021/pr4007365 PMID: 23991618. 3. Jim Manos, Honghua Hu, Barbara R. Rose, Claire E. Wainwright, Iryna B. Zablotska , Joyce Cheney, Lynne Turnbull, Cynthia B. Whitchurch, Keith Grimwood, Christopher Harmer, Snehal N. Anuj, Colin Harbour and the ACFBAL study group. Virulence factor expression patterns in Pseudomonas aeruginosa strains from infants with cystic fibrosis European Journal of Clinical Microbiology and Infectious Diseases 2013: July 7 [Epub ahead of print] DOI: 10.1007/s10096-013-1916-7 PMID: 23832143. 4. Honghua Hu, Christopher Harmer, Snehal Anuj, Claire E. Wainwright, Jim Manos, Joyce Cheney, Colin Harbour, Iryna Zablotska, Lynne Turnbull, Cynthia B. Whitchurch, Keith Grimwood, Barbara Rose. Type 3 secretion system effector genotype and secretion phenotype of longitudinally- collected P. aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening Clinical Microbiology and Infection 2013; 19: 266–272 PMID: 22329595. 5. Christopher J. Harmer, James A. Triccas, Honghua Hu, Barbara Rose, Peter Bye, Mark Elkins and Jim Manos. Pseudomonas aeruginosa strains from the chronically infected cystic fibrosis lung display increased invasiveness of A549 epithelial cells over time Microbial Pathogenesis 2012 53(1) 37-43 PMID 22516803. 6. Nathan J. Hare, Nestor Solis, Christopher Harmer, N. Bishara Marzook, Barbara Rose, Colin Harbour, Ben Crossett, Jim Manos, Stuart Cordwell. Proteomic profiling of Pseudomonas aeruginosa AES-1R, PAO1 and PA14 reveals potential virulence determinants associated with a transmissible cystic fibrosis-associated strain BMC Microbiol. 2012 12(1):16. PMID: 22264352. 7. Nathan J. Hare, Cho Zin Soe, Barbara Rose, Colin Harbour, Rachel Codd, Jim Manos and Stuart J. Cordwell Proteomics of Pseudomonas aeruginosa Australian Epidemic Strain 1 (AES-1), Cultured under Conditions Mimicking the Cystic Fibrosis Lung Reveals Increased Iron Acquisition via the Siderophore Pyochelin Journal of Proteome Research 2012 11: 776- 795. PMID: 22054071. 8. Sharna Naughton, Dane Parker, Torsten Seemann, Torsten Thomas, Lynne Turnbull, Barbara Rose, Peter Bye, Stuart Cordwell, Cynthia Whitchurch and Jim Manos Pseudomonas aeruginosa AES-1 exhibits increased virulence gene expression during chronic infection of cystic fibrosis lung PloS one 2011 6: 9, e24526. PMID: 21935417. 9. Carina Fung, Sharna Naughton, Lynne Turnbull, Pholawat Tingpej, Barbara Rose, Jonathan Arthur, Honghua Hu, Christopher Harmer, Colin Harbour, Daniel J. Hassett, Cynthia B. Whitchurch and Jim Manos Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking CF lung sputum Journal of Medical Microbiology 2010 59: 1089-1100. PMID: 20522626. 10. Pholawat Tingpej, Mark Elkins, Barbara Rose, Honghua Hu, Carmel Moriarty, Jim Manos, Belinda Barras, Peter Bye, and Colin Harbour Clinical profile of adult cystic fibrosis patients with frequent epidemic clones of Pseudomonas aeruginosa 2010 Respirology 15: 923-929. PMID: 20573059. 11. Jim Manos, Jonathan Arthur, Barbara Rose, Scott Bell, Pholawat Tingpej, Honghua Hu, Jeremy Webb, Staffan Kjelleberg, Mark Douglas Gorrell, Peter Bye and Colin Harbour Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth 2009 FEMS Microbiol Lett 292: 107-114. PMID: 19222585. 2 CV - Dr Jim Manos

12. Jim Manos, Jonathan Arthur, Barbara Rose, Pholawat Tingpej, Carina Fung, Michelle Curtis, Jeremy S. Webb, Honghua Hu, Staffan Kjelleberg, Mark D. Gorrell, Peter Bye and Colin Harbour 2008 Transcriptome analyses and biofilm-forming characteristics of a clonal Pseudomonas aeruginosa from the cystic fibrosis lung Journal of Medical Microbiology 2008 57: 1454-1465. 13. Pholawat Tingpej, Lucas Smith, Barbara Rose, Hua Zhu, Tim Conibear, Khaled Al Nassafi, Jim Manos, Mark Elkins, Peter Bye, Mark Willcox, Scott Bell, Claire Wainwright and Colin Harbour. Phenotypic characterization of clonal and non-clonal Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis Journal of Clinical Microbiology 2007 45: 1697-1704. 14. Smith, L., Rose, B., Tingpej, P., Zhu, H. Conibear, T., Manos, J., Bye P., Elkins, M., Willcox, M., Bell S., Wainwright, C. and Harbour, C. Protease IV production in Pseudomonas aeruginosa from the lungs of adults with cystic fibrosis Journal of Medical Microbiology 2006 55: 1641-1644. 15. Manos, J. and Belas, R. 2004 Transcription of Proteus mirabilis flaAB flagellin Microbiology 150: 2857-2863. 16. Manos, J., Artimovich, E. and Belas, R. 2004 Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella. Microbiology 150: 1291-1299. 17. Zhang, D.L*. Manos, J*., Ma, X., Belas R and Karaolis, D.K.R. 2004 Transcriptional analysis and operon structure of the tagA-orf2-orf3-mop-tagD region of the Vibrio pathogenicity island in epidemic Vibrio cholerae. FEMS letters 235: 199-207.* Authors contributed equally. 18. Belas, R., Manos, J. and Suvanasuthi, R. 2004 Proteus mirabilis ZapA metalloprotease degrades a broad spectrum of substrates, including antimicrobial peptides. Infection and Immunity 72: 5159-5167. 19. Prouty, A.C. Brodsky I., Manos, J. Belas, R. Falkow, S., and Gunn, J.S. 2004. Transcriptional regulation of Salmonella enteritica Serovar Typhimurium genes by bile FEMS Immunology and Medical Microbiology 40: 177-185. 20. Manos, J., Kolesnikow, T., and Hazell, S.L. 1998. An investigation of the molecular basis of the spontaneous occurrence of a catalase-negative phenotype in Helicobacter pylori Helicobacter 3: 28-38. 21. Jorgensen, M.A., Manos, J., Mendz, G.L. and Hazell, S.L. 1998. The mode of action of metranidazole in Helicobacter pylori: futile cycling or reduction? J. Antimicrob. Chemother. 41:67- 75.

BOOK CHAPTERS

Manos, J., and Belas, R. ‘The Genera Proteus, Morganella and Providencia’ in M. Dworkin et al., Eds The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 2006 4th edition, Springer-Verlag, New York.

GRANTS (Current)

NHMRC Project Grant #632788 Identification of proteins specific to transmissible Pseudomonas aeruginosa in cystic fibrosis infection Cordwell, C., Manos, J., Harbour C. (2010- 6-30-2013 extended) $427,200AUD.

GRANTS (Prior):

3 CV - Dr Jim Manos

Early Career Researcher (ECR) Grant (University of Sydney) Pseudomonas aeruginosa virulence gene expression in media mimicking cystic fibrosis lung sputum Jim Manos Y2009 $49,450AUD

Australian Cystic Fibrosis Research Trust: Genomics as a tool to study P. aeruginosa lung disease in cystic fibrosis. Beatson, S., Rose, B., Wainwright, C., Manos, J., Harbour, C., Thomas, T., Grimwood, K. Y2009 $79,500AUD.

Australian Cystic Fibrosis Research Trust: Establishing the prevalence of clonal Pseudomonas aeruginosa strains in cystic fibrosis clinics across Australia. Bye, P., Bell, S.C., Rose, BR., Harbour, C., Marks, GB., Robinson, PJ., and Manos, J. Y2008 $67,750AUD.

Australian Cystic Fibrosis Research Trust Pseudomonas aeruginosa in cystic fibrosis: determinants of acquisition, persistence and virulence. Rose, BR., Wainwright, C., Whitchurch, C., Manos, J., Harbour, C., Bye, P., Turnbull, L., Armstrong, D., Grimwood, K. and Nissen, M. Y2007 $78,660AUD

Australian Cystic Fibrosis Research Trust Sequencing of transmissible Pseudomonas aeruginosa from patients with cystic fibrosis Harbour, C., Rose, BR., Manos, J., Arthur, J. and Bye, P. Y2006 $78,700AUD.

University of Sydney Bridging Grant An integrated transcriptomic and proteomic approach to the characterisation of epidemic Pseudomonas aeruginosa. Rose, BR., Cordwell, S.C., Willcox, M., Webb, J.S., Manos, J., Bye, P., Harbour, C. and Bell, S.C. Y2006 $23,000AUD

Australian Cystic Fibrosis Research Trust Transmission of Pseudomonas aeruginosa: clinical and molecular studies, Rose, BR., Wainwright, C., Manos, J., Bye, P., Bell, S., Harbour, C., Nissen, M., Sloots, T. and Coulter, C. Y2005 $74,740AUD.

PATENTS:

Australian patent: No 2005905356 (Filed: 28-9-05, Granted 28-9-06): Assessment of Infectious Bacteria Inventors: Manos, J., Arthur, J.A., Rose, B. and Harbour, C.

HONOURS AND AWARDS

2013 Overseas travel grant by FEMS) to attend Pseudomonas2013 in Lausanne Switzerland.

1997 Young Scientist Award, by The European Helicobacter pylori Study Group (EHPSG).

1996 Postgraduate Travel Award, by: School of Microbiology and Immunology University of New South Wales.

1994 Academic Exchange recipient: (Faculty of Biological Sciences, UNSW); for research in Department of Microbiology, Seoul National University, Seoul, South Korea. 4 CV - Dr Jim Manos

1993 Student Travel Award: The Australian Society for Microbiology (ASM). Awarded towards travel and participation in the ASM annual general meeting

1992 Australian Postgraduate Research Award (APRA), 4-year postgraduate scholarship. Awarded by the Australian Federal Government, Canberra, ACT Australia.

INVITED SPEAKER AND PUBLISHED ABSTRACTS (First/senior author only)

Conference: Pseudomonas 2011 International Conference, Sydney (2011).”Genomic features and gene expression differences between acute and chronic infection isotypes of Pseudomonas aeruginosa Australian Epidemic Strain-1” Presentation: Poster presentation

Conference: Pseudomonas 2011 International Conference, Sydney (2011).”Characterisation of persistent P. aeruginosa strains and their immune-phenotype in an in vivo model of lung infection” Presentation: Poster presentation

Conference: Australian Society for Microbiology (ASM) General Meeting, Hobart (2011). “Pseudomonas aeruginosa AES-1 exhibits greater virulence gene expression during chronic infection compared to non-epidemic strains” Presentation: Invited Symposium speaker

Conference: Universiti Malaysia Sabah Biotechnology Symposium IV Kota Kinabalu Malaysia (2010). “Genomic and gene expression changes in sequential acute and chronic infection isolates of Pseudomonas aeruginosa AES-1 from CF patients” Presentation: Invited plenary speaker

Conference: Australian Society for Microbiology (ASM) General Meeting, Sydney (2010). “Gene expression changes in sequential acute and chronic infection isolates of Pseudomonas aeruginosa AES-1 from a patient with cystic fibrosis” Presentation: Proffered paper

Conference: Pseudomonas 2009 Hannover Germany (2009): “Gene expression characteristics of a virulent isolate of Pseudomonas aeruginosa grown in artificial sputum medium”. Presentation: Poster Presentation

Conference: BacPath 9 Conference: The Molecular Biology of Bacterial Pathogens Lorne Victoria (2007). “Enhanced Biofilm Formation and Genetic Downregulation by a Transmissible Pseudomonas aeruginosa from Cystic Fibrosis Patients” Presentation: Poster Presentation

Conference: 21st Annual North American Cystic Fibrosis Conference (NACFC) Anaheim CA USA (2007). “Enhanced Biofilm Formation and Genetic Downregulation by Transmissible Pseudomonas aeruginosa from Cystic Fibrosis Patients” 5 CV - Dr Jim Manos

Presentation: Poster presentation

Conference: 19th Annual North American Cystic Fibrosis Conference (NACFC) Baltimore MD USA (2005). ‘Transmissible Planktonic Pseudomonas aeruginosa from Cystic Fibrosis Patients Exhibits Gene Expression Consistent with a Biofilm Mode of Growth” Presentation: Proffered paper

Conference: Australian Society for Microbiology (ASM) General Meeting, Canberra (2005). ‘Transmissible Planktonic Pseudomonas aeruginosa from Cystic Fibrosis Patients Exhibits Gene Expression Consistent with a Biofilm Mode of Growth’ Presentation: Proffered paper

Conference: Australia-New Zealand Society for Microbiology (ASMNZ) General Meeting, Auckland, NZ (2003). ‘Hybrid flaAB mRNA transcription in Proteus mirabilis’ Presentation: Proffered paper

Conference: American Society of Microbiology (ASM) General Meeting, Washington DC (2003) ‘Enhanced motility of a Proteus mirabilis strain expressing hybrid FlaAB flagella’ Presentation: Poster presentation Conference: American Society of Microbiology (ASM) General Meeting, Orlando, Florida (2001). ‘Flagellin gene conversion in Proteus mirabilis’ Presentation: Poster presentation

Conference: Bacterial Locomotion and Sensory Transduction VI (BLAST VI) Cuernavaca, Mexico (2001). ‘Analysis of the frequency of flagellin gene conversion in Proteus mirabilis using a flaB::lacZ reporter fusion’ Presentation: Poster presentation

Conference: Gordon Research Conference: Sensory Transduction in Microorganisms, Ventura, California USA (2000). ‘Sequence analysis of the fliA locus of Proteus mirabilis’ Presentation: Poster presentation

Conference: Australian Society of Microbiology (ASM), Hobart (1998). ‘Does Helicobacter pylori possess an inducible catalase? -Evidence for a novel catalase-like gene in H. pylori’. Presentation: Proffered paper

Conference: American Society of Microbiology (ASM), Miami Beach USA (1997). ‘Sequencing of the katA gene of Helicobacter pylori’ Presentation: Poster presentation

Conference: IXth International Conference on Gastroduodenal pathology and Helicobacter pylori, Copenhagen (1996). ‘The molecular basis of a catalase-negative phenotype of Helicobacter pylori’ 6 CV - Dr Jim Manos

Presentation: Poster presentation

Conference: Australian Society of Microbiology (ASM), Perth (1993). ‘Catalase-negative Helicobacter pylori - Evidence for catalase as a virulence factor?’ Presentation: Proffered paper

Conference: Australian Society of Microbiology (ASM), Sydney (1992). ‘The purification and characterisation of Helicobacter felis catalase’ Presentation: Proffered paper.

POSTGRADUATE SUPERVISION

University of Sydney PhD

2009-present Chief Supervisor PhD student Christopher Harmer 2005-present Co-supervisor: PhD student Khaled Al-Nassafi

University of Sydney Honours

2009 Supervisor Honours student Sharna Naughton BMedSci (Awarded 1st Class) 2008 Supervisor INIM Honours student Christopher Harmer (Passed –not graded) 2007 Supervisor Honours student: Carina Fung BMedSci (Awarded 1st Class). 2006 Supervisor Honours student: Michelle Curtis BMedSci (Awarded 1st Class)

University of Maryland Biotechnology Institute USA 2002-2003 Supervisor Science intern student: Elena Artimovich (UMBI) 2000-2002 Co-supervisor MSc student: Qian Wang (UMBI) 1999-2003 Co-supervisor PhD student: Todd R. Miller (UMBI).

GRADUATE and UNDERGRADUATE TEACHING 2007-present Lecturer in Infectious Diseases INIM5011, INFD3012, INIM5012 2004-2007 Lectures & practical classes INFD3012

University of New South Wales 1993–1997 Tutor & Demonstrator General and Medical Microbiology (UNSW)

STATEMENT OF ACCOMPLISHMENTS

University of Sydney, Department of Infectious Diseases: 2004-present

 I have conducted genomic expression analysis of transmissible strains of P. aeruginosa in cystic fibrosis patients using full microarray profiling. To this end, I have:

7 CV - Dr Jim Manos

 Established and optimised techniques for P. aeruginosa RNA extraction, cDNA synthesis, labelling and analysis of output profiles to detect putative virulence determinants as targets for mutagenesis.  Investigated the genomes of several P. aeruginosa strains for the presence of putative horizontally acquired virulence genes such as pilC, flaA, flgK, flgL and the bacteriophage pf4.  Closely involved in the genome sequencing of the “early infection” epidemic isolate AES-1R, and have validated for use an artificial sputum medium (ASMDM) to mimic growth in CF sputum and a non-redundant array of eight sequenced P. aeruginosa genomes.  Analysed the transcriptome of sequential “early” and ‘chronic’ infection isolates from the same patient for expression of genes that may harbour virulence, persistence or transmissibility determinants.  Involved in the genomic sequencing of three sets of sequential isolates in collaboration with Dr Scott Beatson at the University of Queensland.

UMBI: 2000-2003

 In the laboratory of Associate Professor Robert Belas I elucidated the mechanism of expression of hybrid forms of the two flagellin-encoding genes of Proteus mirabilis. In the course of these studies, I:   Constructed lacZ fusions and measured the expression of the wild type and hybrid genotypes.  Established the existence of a flaAB hybrid mRNA transcript and FlaAB product, determined the frequency of flaAB hybrid formation and developed a putative mechanism for flaAB transcription. This work has been published in Microbiology (Manos & Belas 2004).  Identified differences in motility between wild type and phase-locked flagellar mutants, resulting in a publication in Microbiology (Manos, Artimovich & Belas 2004).  Identified and sequenced the alternative σ28 regulator of flagellin gene expression (fliA), in this species.  Concurrent with the flagellin work, I investigated expression of the IgG-degrading metalloprotease ZapA by P. mirabilis and its effect in cleaving a range of host proteins. This work has been published in Infection and Immunity (Sept. 2004).  I have demonstrated an ability to work independently, including collaboration with two other laboratories on S. typhimurium motility and the VPI operon of V. cholerae respectively, resulting in two publications (May and June 2004).  I have further developed my teaching skills as a mentor and written a chapter for a reference text (published 2001).  I played a major role as Co-Principal Investigator in drafting an NSF grant together with Dr Belas (2002).  I have developed proficiency in many techniques, including Southern and Northern Blot and hybridisation, recombinant DNA cloning, DNA sequencing, RT-PCR, fluorescent antibody detection of protein expression and phylogenetic analysis of identified genes.

UNSW: 1993-1999

8 CV - Dr Jim Manos

The catalase of Helicobacter pylori is an important virulence factor in gastric pathogenesis. To further understand the mechanism controlling catalase expression, I undertook the sequencing and characterization of the H. pylori katA gene in KatA- strains and compared them to wild type. In the corse of this work I also:

 Identified that lack of katA transcription is most likely due to Fur (iron binding) protein repression.  Sequenced and characterized adjacent genes in the operon, including phnA and a gene involved in regulation of iron uptake resulting in two publications, (Helicobacter and J. Antimicrobial Chemotherapy, 1998).  Mastered a number of important techniques, including 2-D gel electrophoresis, Sanger dideoxy sequencing, PCR, Western blot, column separation of proteins, enzyme kinetics and phylogenetic analysis of sequenced genes by comparison across bacterial species.  Developed proficiency in seminar presentation in the process of explaining my research at scientific conferences and meetings.  Developed teaching skills as a tutor and demonstrator.

OTHER SIGNIFICANT SEMINARS Venue: Department of Chemistry and Biomolecular Sciences, Macquarie University, North Ryde (November 2011). Subject “What drives persistent Pseudomonas aeruginosa in CF lung?” Presentation 1 hour seminar

Venue: Discipline of Infectious Diseases and Immunology, University of Sydney (July 2007). Subject: “Virulent Mucus-Dwelling Bacteria-A Journey through the Human Body” Presentation: 1 hour seminar

Venue Prince of Wales Hospital Department of Respiratory Medicine (November 2005). Subject: “Genomic profiling of transmissible CF clonal strains of Pseudomonas aeruginosa” Presentation: 1 hour seminar Venue: Discipline of Infectious Diseases and Immunology, University of Sydney (April 2004). Subject: “Flagellin gene switching in Proteus mirabilis” Presentation: 1 hour seminar Venue: Center of Marine Biotechnology Scientific Retreat, MD. (Aug. 2003). Subject: “Swimming against the tide: The hybrid flagellin of Proteus mirabilis” Presentation: 1 hour seminar Venue: Center of Marine Biotechnology Scientific Retreat, MD. (Sept 2000). Subject: “Genetic aspects of motility regulation in Proteus mirabilis” Presentation: 20 min seminar. Venue: Center of Marine Biotechnology Scientific Retreat, MD. (Sept 1999). Subject: “Flagellin gene rearrangement in Proteus mirabilis” Presentation: 20 min seminar. Venue: Chesapeake Area Microbial Pathogenesis (CAMP) meeting, Rockville, MD. (June, 1999). Subject; “The flagellin genes of Proteus mirabilis” 9 CV - Dr Jim Manos

Presentation: 20 min seminar at the annual area meeting of microbial pathogenesis researchers. Venue: School of Microbiology and Immunology UNSW (Nov 1998). Subject: “Molecular characterisation of Helicobacter pylori catalase and analysis of the basis of a spontaneous catalase negative mutant”. Presentation: 60-minute seminar. Venue: School of Microbiology and Immunology UNSW, Sydney (Mar 1996). Subject: “The technique of Cleavase Fragment Length Polymorphism (CFLP)-PCR and its application in research”. Presentation: 50 min presentation to the Helicobacter pylori study group. Venue: School of Microbiology and Immunology UNSW, Sydney (May 1995). Subject: “A Seminar encompassing an 18-month review of my postgraduate research”. Presentation: 60 min seminar. Venue: Dept of Microbiology, Seoul National University, South Korea (Nov l994) Subject: “The catalase of Helicobacter pylori and the existence of spontaneous catalase-negative mutants”. Presentation: 50 min presentation. Venue: School of Microbiology and Immunology UNSW, Sydney (Feb 1994) Subject: “A guided tour of the 'Cary 11 Spectrophotometer how to make best us of its potential”. Presentation: Special tutorial/seminar. Venue: School of Microbiology and Immunology UNSW, Sydney (Nov 1991) Subject: “Purification and characterisation of Helicobacter felis catalase”. Presentation: 60 min seminar (Honours defence).

PROFESSIONAL DEVELOPMENT 2006 Sydney-Learning Course: “The New Supervisor” Sept 25-27th 2006. 2007 Early Career Researcher Course Sydney University 2008 Go8 Course: Sydney University 2009 Institute for Teaching and Learning, Sydney University: Research Higher Degree supervision course 2010 PROFESSIONAL POSITIONS 2008-present Coordinator Graduate Studies in Infection and Immunity (Postgraduate Masters Program). 2000-2001 President, Postdoctoral Association, Center of Marine Biotechnology, Baltimore, MD, USA 2002 and 2003 ASM (USA) Judge (Head of panel) at the Maryland Science Fair, Towson University, Baltimore MD USA. 2005 NHMRC Project Grant reviewer.

PROFESSIONAL MEMBERSHIP 2012-present Treasurer of NSW branch of ASM 2011-present Committee member of NSW branch of ASM 2004-present Member: Australian Society for Microbiology (MASM). 2000-present American Society of Microbiology (ASMUSA), Washington, DC, USA