Curriculum Vitae s431

CURRICULUM VITAE RNDr. Monika Dolejská, Ph.D.

PERSONAL DATA : Date of Birth: 12th October 1982, Nationality: Czech, Marital Status: Single

PREVIOUS EMPLOYMENT AND CURRENT POSITION : Since 2014: Senior Researcher, Central European Institute of Technology (CEITEC) 2011-2014: Junior Researcher, CEITEC 2006-2014: Assistant Professor, Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno (UVPS) 2006-2014: External tutor, Faculty of Science, Masaryk University (MU)

EDUCATION AND PROFESSIONAL SKILLS : Since 2012: Head of Laboratory of Molecular Biology at Department of Biology and Wildlife Diseases 2009: PhD studies in Veterinary Hygiene and Ecology, Faculty of Veterinary Hygiene and Ecology, UVPS 2006: Master in Microbiology, Faculty of Science, MU 2003: Bachelor in General Biology, Faculty of Science, MU

TEACHING EXPERIENCE: Since 2006: Supervisor of Bachelor, Master, PhD students (45 students) Biology and Genetics course I, II for Bachelor and Master students of UVPS Laboratory courses of isolation and characterization of antimicrobial resistant at UVPS Since 2014: Antibiotic Resistance course for Master students of UVPS

I NTERNSHIPS : 2014 (3 months): Postdoc Fellowship in Molecular Microbiology, Department of Veterinary Disease Biology, University of Copenhagen, Denmark 2011-2012 (14 months): Fellowship in Plasmid Biology and New-Generation Sequencing, Department of Microbiology, Immunology and Infectious Diseases, Istituto Superiore di Sanità, Rome, Italy 2009 (6 months): Guest PhD student in Molecular Epidemiology, Division for Epidemiology and Microbial Genomics, National Food Institute, Technical University of Denmark

INVESTIGATOR/PI OF GRANTS:  Internal Grant Agency of Ministry of Health Nr. NT/14398 - Bacterial resistance to fluoroquinolones, possibilities for its prevention and public health protection (2013-2015)  Central European Institute of Technology (CEITEC) - Molecular Veterinary Medicine - Molecular epidemiology and genetic background of antimicrobial-resistant bacteria (2012-present)  Czech Science Foundation Nr. GPP502/10/P083 - Characterization of coliform bacteria resistant to cephalosporins and the risk assessment of antimicrobial usage for their selection (2010-2012)  National Agency for Research in Agriculture Grant Nr. NAZV QH71057 - Monitoring of the koi herpes virus disease (KHVD) occurrence in breeds of common card in CR and testing of susceptibility of chosen strains of common carp to KHV. Antimicrobial resistance in fish pathogens (2007-2014)  Ministry of Education, Youth and Sports of the Czech Republic Grant (MSM6215712402): Food Hygiene and Safety (2007 – 2011)

AWARDS:

1 2014: Nomination for Women In Science Czech Republic sponsored by L’Oréal, UNESCO and supported by Czech Academy of Science. Prestigious award for recognized young female scientists under the age of 35. 2013: Research Excellence Award from the President of Czech Science Foundation for extraordinary results of Research Grant funded by Czech Science Foundation (GPP502/10/P083) 2013: Research Excellence Award from Czech Society for Clinical Microbiology for outstanding results in the field of clinical microbiology and antibiotic resistance 2010: Dean's Graduate Research Excellence Award for research achievements during PhD studies at UVPS TECHNICAL SKILLS : Fundamental microbiology: Extensive experience in Microbiology techniques. Isolation, identification and characterization of bacteria (Enterobacteriaceae, Aeromonas, enterococci). Antibiotic susceptibility testing. Molecular Biology: DNA and RNA isolation, DNA cloning and construct design. RT-PCR. DNA and RNA Sequencing. Site-directed mutagenesis (disruption, deletion and PCR mutagenesis). Plasmid analysis (replicon typing, RFLP, plasmid MLST). Transformation and expression of heterologous genes in different type of hosts. GFP expression and detection. Metagenomic library. Clone library. Plasmid DNA library construction and 454 Genome Sequencer FLX/Junior procedure. Blotting and hybridization techniques. Enzymatic assays. Molecular typing of bacteria (PFGE, MLST, virulence genes). Flow cytometry and epifluorescent microscopy. Bioinformatics/softwares: Bioinformatics tools for the analysis and comparison of DNA and protein sequences and network programs available in EMBL, NCBI. Genome assembly and annotation. Artemis, DNAMAN, Bionumerics, MEGA, Bioedit, Genome Matcher.

ACHIEVEMENT TRACK-RECORD: Number of Publications in the Journals with IF: 34 H-index: 11 Nr. of Citations: 219 (Web of Science, without self-citations)

Citation Analysis (Web of Science, 10.8.2014)

LIST OF PUBLICATIONS IN JOURNALS WITH IF:

1. DOLEJSKA, M., CIZEK, A., LITERAK, I. (2007) High prevalence of antimicrobial-resistant genes and integrons in Escherichia coli isolates from Black-headed Gulls in the Czech Republic. Journal of Applied Microbiology 103, 11-19. 2. ČÍŽEK, A., DOLEJSKÁ, M., KARPÍŠKOVÁ, R., DĚDIČOVÁ, D., LITERÁK, I. (2007) Wild black- headed gulls (Larus ridibundus) as an environmental reservoir of Salmonella strains resistant to antimicrobial drugs. European Journal of Wildlife Research 53, 55-60.

2 3. LITERÁK, I., VANKO, R., DOLEJSKÁ, M., ČÍŽEK, A., KARPÍŠKOVÁ, R. (2007) Antibiotic resistant Escherichia coli and Salmonella in Russian rooks (Corvus frugilegus) wintering in the Czech Republic. Letters in Applied Microbiology 45, 616-621. 4. ČÍŽEK, A., DOLEJSKÁ, M., NOVOTNÁ, R., HAAS, D., VYSKOČIL, M. (2008) Survey of Shiga toxigenic Escherichia coli O157 and drug-resistant coliform bacteria from in-line milk filters on dairy farms in the Czech Republic. Journal of Applied Microbiology 104, 852-860. 5. DOLEJSKÁ, M., ŠENK, D., ČÍŽEK, A., RYBAŘÍKOVÁ, J., SYCHRA, O., LITERÁK, I. (2008) Antimicrobial resistant Escherichia coli isolates in cattle and house sparrows on two Czech dairy farms. Research in Veterinary Sciences 85, 491-494. 6. DOLEJSKÁ, M., BIEROŠOVÁ, B., KOHOUTOVÁ, L., LITERÁK, I., ČÍŽEK A. (2009) Antibiotic- resistant Salmonella and Escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls. Journal of Applied Microbiology 106, 1941-1950. 7. LITERAK, I., DOLEJSKA, M., RYBARIKOVA, J., CIZEK, A., STREJCKOVA, P., VYSKOCILOVA, M., FRIEDMAN, M., KLIMES, J. (2009) Highly Variable Patterns of Antimicrobial Resistance in Commensal Escherichia coli Isolates from Pigs, Sympatric Rodents and Flies. Microbial Drug Resistance 15, 229-237. 8. LITERAK, I., DOLEJSKA, M., CIZEK, A., DJIGO, C. A. T., KONECNY, A., KOUBEK, P. (2009) Reservoirs of antibiotic-resistant Enterobacteriaceae among animals sympatric to humans in Senegal: extended-spectrum beta-lactamases in bacteria in a black rat (Rattus rattus). African Journal of Microbiology Research 11, 751-754. 9. LITERAK, I., DOLEJSKA, M., RADIMERSKY, T., KLIMES, J., FRIEDMAN, M., AARESTRUP, F. M., HASMAN, H., CIZEK, A. (2010) Antimicrobial-resistant faecal Escherichia coli in wild mammals in central Europe: multiresistant E. coli producing extended-spectrum beta-lactamases in wild boars. Journal of Applied Microbiology 108, 1702-1711. 10. ČÍŽEK, A., DOLEJSKÁ, M., SOCHOROVÁ, R., STRACHOTOVÁ, K., PIAČOVÁ, V., VESELÝ, T. (2010) Antimicrobial resistance and its genetic determinants in aeromonads isolated in ornamental (koi) carp (Cyprinus carpio koi) and common carp (Cyprinus carpio). Veterinary Microbiology 142, 435-439. 11. RYBARIKOVA, J., LITERAK, I., DOLEJSKA, M., MATERNA, D., CIZEK, A. (2010) Phenotypic and genotypic characteristics of antimicrobial resistant Escherichia coli isolated from symbovine flies, cattle and sympatric insectivorous house martins from a farm in the Czech Republic (2006-2007). Research in Veterinary Sciences 89, 179-183. 12. RADIMERSKY, T., FROLKOVA, P., JANOSZOWSKA, D., DOLEJSKA, M., SVEC, P., ROUBALOVA, E., CIKOVA, P., CIZEK, A., LITERAK, I. (2010) Antibiotic resistance in faecal bacteria (Escherichia coli, Enterococcus spp.) in feral pigeons. Journal of Applied Microbiology 109, 1687-1695. 13. LITERAK, I., DOLEJSKA, M., JANOSZOWSKA, D., HRUSAKOVA, J., WLODZIMIERZ, M., RZYSKA, H., BZOMA, S., CIZEK, A. (2010) Antibiotic-resistant Escherichia coli, including strains with the extended-spectrum beta-lactamase and qnrS genes, on the Baltic seacoast of Poland: Spreading of emerging resistant strains by waterbirds. Applied and Environmental Microbiology 76, 8126-8134. 14. DOLEJSKA, M., DUSKOVA, E., RYBARIKOVA, J., JANOSZOWSKA, D., ROUBALOVA, E., DIBDAKOVA, K., MASECKOVA, G., KOHOUTOVA, L., LITERAK, I., SMOLA, J., CIZEK, A. (2011)

Plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli isolates from equine clinic and horseback riding centre. Journal of Antimicrobial Chemotherapy 66, 757-764. 15. DOLEJSKA, M., JURCICKOVA, Z., LITERAK, I., POKLUDOVA, L., BURES, J., KOHOUTOVA, L.,

SMOLA, J., CIZEK, A. (2011) IncN plasmids carrying blaCTX-M-1 in Escherichia coli isolates from cows on a farm where ceftiofur, cefoperazone and cefquinome were commonly used. Veterinary Microbiology 149, 513-516. 16. LITERAK, I., PETRO, R., DOLEJSKA, M., GRUBEROVA, E., DOBIASOVA, H., PETR, J., CIZEK, A.

3 (2011) Antimicrobial resistance in fecal Escherichia coli isolates from healthy urban children of two age categories with a view to their antibiotic therapy. Antimicrobial Agents and Chemotherapy, 55, 3005-3007. 17. DOLEJSKA , M., MATULOVA, M., KOHOUTOVA, L., LITERAK, I., BARDON, J., CIZEK, A. (2011) Extended-spectrum beta-lactamase-producing Escherichia coli in turkey meat production

flocks in the Czech Republic: National survey reveals widespread isolates with blaSHV-12 genes on IncFII plasmids. Letters in Applied Microbiology 53, 271-277. 18. DOLEJSKA, M., FLOREK, M., FLORKOVA, P., JAMBOROVA, I., PURGERTOVA, M., KUTILOVA, I., CIZEK, A., GUENTHER, S., LITERAK, I. (2011) CTX-M-15-producing Escherichia coli clone B2- O25b-ST131 and Klebsiella spp. isolates in municipal wastewater treatment plant effluents. Journal of Antimicrobial Chemotherapy 66, 2784-2790. 19. DUBSKA, L., PILATOVA, K., DOLEJSKA, M., BORTLICEK, Z., FROSTOVA, T., LITERAK, I., VALIK, D. (2011) Surface-enhanced laser desorption ionization/time-of-flight (SELDI-TOF) mass spectrometry (MS) as a phenotypic method for rapid identification of antibiotic resistance. Anaerobe 17, 444-447. 20. ALBRECHTOVA, K., DOLEJSKA, M., CIZEK, A., TAUSOVA, D., KLIMES, J., BEBORA, L., LITERAK, I. (2012) Dogs of nomadic pastoralists in northern Kenya are reservoirs of plasmid-mediated cephalosporin- and quinolone-resistant Escherichia coli, including pandemic clone B2-O25- ST131. Antimicroial Agents and Chemotherapy 56, 4013-4017. 21. TAUSOVA, D., DOLEJSKA, M., CIZEK, A., HANUSOVA, L., HRUSAKOVA, J., SVOBODA, O., CAMLIK, G., LITERAK, I. (2012) Escherichia coli with extended-spectrum β-lactamase and plasmid-mediated quinolone resistance genes in great cormorants and mallards in Central Europe. Journal of Antimicrobial Chemotherapy 67, 1103-1107. 22. LITERAK, I., MICUDOVA, M., TAUSOVA, D., CIZEK, A., DOLEJSKA, M., PAPOUSEK, I., PROCHAZKA, J., VOJTECH, J., BORLEIS, F., GUARDONE, L., GUENTHER, S., HORDOWSKI, J., LEJAS, C., MEISSNER, W., MARCOS, BF., TUCAKOV, M. (2012) Plasmid-Mediated Quinolone Resistance Genes in Fecal Bacteria from Rooks Commonly Wintering Throughout Europe. Microbial Drug Resistance 18, 567-573. 23. DOLEJSKA, M., BRHELOVA, E., DOBIASOVA, H., KRIVDOVA, J., JURANKOVA J., SEVCIKOVA, A., DUBSKA, L., LITERAK, I., CIZEK, A., VAVRINA, M., KUTNIKOVA, L., STERBA, J. (2012) Dissemination of IncFIIK-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from children in hospital paediatric oncology wards. International Journal of Antimicrobial Agents, 40: 510-515. 24. DOLEJSKA, M., VILLA, L., HASMAN, H., HANSEN, L., CARATTOLI, A. (2013) Characterization of

IncN plasmids carrying blaCTX-M-1 and qnr genes in Escherichia coli and Salmonella from animals, environment and humans. Journal of Antimicrobial Chemotherapy, 68: 333-339. 25. DOLEJSKA, M., VILLA, L., POIREL, L., NORDMANN, P., CARATTOLI, A. (2013) Complete sequencing of an IncHI1 plasmid encoding the carbapenemase NDM-1, the ArmA 16S RNA methylase and a resistance-nodulation-cell division/multidrug efflux pump. Journal of Antimicrobial Chemotherapy, 68: 34-39. 26. LITERAK, I., REITSCHMIED, T., BUJNAKOVA, D., DOLEJSKA, M., CIZEK, A., BARDON, J., POKLUDOVA, L., ALEXA, P., HALOVA, D., JAMBOROVA, I. (2013) Broilers as a Source of Quinolone-Resistant and Extraintestinal Pathogenic Escherichia coli in the Czech Republic, Microbial Drug Resistance, 19: 57-63. 27. DOLEJSKA, M., VILLA, L., DOBIASOVA, H., FORTINI, D., FEUDI, C., CARATTOLI, A. (2013) Plasmid content of a clinically relevant Klebsiella pneumoniae clone from the Czech Republic, producing CTX-M-15 and QnrB1. Antimicrobial Agents and Chemotherapy, 57: 1073-1076.

4 28. KLIMES, J., MACHALKOVA, M., DOLEJSKA, M., CIZEK, A., JANOSZOWSKA, D., ALEXA, P., ALBRECHTOVA, K., VOJTECH, J., LITERAK, I. (2013) Escherichia coli-producing exteded- spectrum beta-lactamases CTX-M-15 in a captive South American tapir (Tapirus terrestris). Journal of Zoo and Wildlife Medicine, 44: 173-175. 29. ACCOGLI, M., FORTINI, D., GIUFRÈ, M., GRAZIANI, C., DOLEJSKA, M., CARATTOLI, A., CERQUETTI, M. (2013) IncI1 plasmids associated with the spread of CMY-2, CTX-M-1 and SHV-12 in Escherichia coli of animal and human origin. Clinical Microbiology and Infection. 2013, 19 :E238-240. 30. DOBIASOVA, H., DOLEJSKA, M., JAMBOROVA, I., BRHELOVA, E., BLAZKOVA, L., PAPOUSEK, I., KOZLOVA, M., KLIMES, J., CIZEK, A., LITERAK, I. (2013) Extended spectrum beta-lactamase and fluoroquinolone resistance genes and plasmids among Escherichia coli isolates from zoo animals, Czech Republic. FEMS Microbiology Ecology, 85: 604-611. 31. VILLA, L., CAPONE, A., FORTINI, D., DOLEJSKA, M., RODRÍGUEZ, I., TAGLIETTI, F., DE PAOLIS, P., PETROSILLO, N., CARATTOLI, A. Reversion to susceptibility of a carbapenem-resistant clinical isolate of Klebsiella pneumoniae producing KPC-3. (2013) Journal of Antimicrobial Chemotherapy, 68: 2482-2486. 32. JANATOVA, M., ALBRECHTOVA, K., PETRZELKOVA, K. J., DOLEJSKA, M., PAPOUSEK, I., MASARIKOVA, M., CIZEK, A., TODD, A., SHUTT, K., KALOUSOVA, B., PROFOUSOVA, I., MODRY, D., LITERAK, I. (2014) Antimicrobial-resistant Enterobacteriaceae from humans and wildlife in Dzanga-Sangha Protected Area, Central African Republic. Veterinary Microbiology, 171:422-431. 33. DOBIASOVA, H., KUTILOVA, I., PIACKOVA, V., VESELY, T., CIZEK, A., DOLEJSKA, M. (2014) Ornamental fish as a source of plasmid-mediated quinolone resistance genes and antibiotic resistance plasmids. Veterinary Microbiology, 171:413-421. 34. ALBRECHTOVA, K., KUBELOVA, M., MAZANCOVA, J., DOLEJSKA, M., LITERAK, I., CIZEK, A. (2014) High Prevalence and Variability of CTX-M-15-Producing and Fluoroquinolone- Resistant Escherichia coli Observed in Stray Dogs in Rural Angola. Microbial Drug Resistance, 20:372-375. 35. DOLEJSKA, M., VILLA, L., MINOIA, M., GUARDABASSI, L., CARATTOLI, A. (2014) Complete

sequences of IncHI1 plasmids carrying blaCTX-M-1 and qnrS1 in equine Escherichia coli provide new insights into plasmid evolution. Journal of Antimicrobial Chemotherapy, 69:2388-2393.