A Location Corresponds to Position Within the Strain H16 Genome
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Table S1. Oligonucleotide primer sequences utilized to generate cDNA clones encompassing the full-length genome of the TGEV virus Primer name Primer sequence (5'-3') Locationa TGEV1F GATGGCTACATGCTGACAGCCTA b TGEV1R ATATATTGATCAACATATATGGCACC 667 TGEV2F TTAAAGTAAAGTGAGTGTAGCGTG 5 TGEV2R CATGGAGTCTTCTTGAATAATAGA 1477 TGEV3F TAAGCCTGGTGATGCTGTTGTC 1136 TGEV3R CCGATCTCAATATCATTGCGAG 2635 TGEV4F ATGATGGTGATATTGATGCTGC 2146 TGEV4R TGAGAGTCGTTGTCTTGAAAGG 3555 TGEV5F GTGCTGAAGGGATTTCTTCTCA 3370 TGEV5R GTCACCATTACGTGGTCCAACT 4556 TGEV6F CAATTTCTTGGAACCTGATGTTAT 4340 TGEV6R CAGCAACCTTAGAATTTTATCTCC 5831 TGEV7F CACAAGCAGAGGAAAGACCTAAGA 5656 TGEV7R TTGTTGACAGCCATTTCAAATGTA 7141 TGEV8F TAACAGTGATTTGCTTGAAGACCT 6962 TGEV8R GACACATTGTTCACAACAAGAGTG 8449 TGEV9F TGTGGTAATTCTGTGCTAGGATTC 8232 TGEV9R GAGCCATTTCCAAGTTCTAAGTGA 9457 TGEV10F TGGTTTATGGTTAGGAGATGAAGT 9032 TGEV10R AGAAAAGTCCCAGAACAAATTGTG 10109 TGEV11F TCTTTATGTACACACCCTTCACGT 9928 TGEV11R GAACATTGTTTTCCTGCCTAATCT 11427 TGEV12F AGTCAAAGATTGTTTCTGCTATGC 11224 TGEV12R CATTACCGAACTCACATCTACCCT 12635 TGEV13F AGGAATTTGGACAAACATGACG 12467 TGEV13R CTGTACGAGCTCTTGCCTTACC 13967 TGEV14F TTTGAATGTTATGACGGTGGGT 13736 TGEV14R CACAACGAAGTACAGTTTGCGA 15143 TGEV15F GCATACCCACTTACAAAACACCCT 14894 TGEV15R AAAGTTCTAGGAGCTGGTAGCTGC 16329 TGEV16F TCAATGCTCTACCAGAAGTAAGTT 16176 TGEV16R GTATGTCAATGCAGTAAGGGTTAT 17621 TGEV17F TGGTGGTCTTGAACTTACAACTAT 17452 TGEV17R ATTAGAAATAAGCACTGCATCTCT 18859 TGEV18F CAAAACGCAAACTTGGACTCACAC 18624 TGEV18R TCGGAAACGTAATCTCTCAAATCA 19779 TGEV19F GTAAGGCATGGAGATGGATGTTGT 19398 TGEV19R CTGTGGTGGTAGTAGGTGGTGAGC 20791 TGEV20F GCTCACCACCTACTACCACCAC 20768 TGEV20R CCAACAGGACTCAACGACAAAC 22265 TGEV21F TCTTGGTATGAAGCGTAGTGGTTA 21972 TGEV21R CAATTTAAGGGCATTTTCAGAAAC 23103 TGEV22F TTAGCACTGGTAACGTCACGAT 22850 TGEV22R TTCATAAGCCGTTGGTAATAGC 24045 TGEV23F ATTTCCAAGCCATTAGTAGTTCTA 23732 TGEV23R TATGATGCACATTTGCTGTGTTAT 25181 TGEV24F GCTGTACTTGACGAACTTGATTGT 24850 TGEV24R CTGAATTGAGGTCTTCCATATTGT 26303 TGEV25F GTTATTATTGTTCCAGCGCAACATG 25864 TGEV25R TGGAAGAGAACTGCAGGTAAAGGTG 27359 TGEV26F GTACCCAAAGGAATAGGTAACAGG 27040 TGEV26R AGACTAGAAAGATTAATCGAAGCA 28221 TGEV27F ACACCTAAGAATGAAAACAAACACAC 27903 TGEV27R TACCGTCGTTCCACTAGTGATTT c a Location corresponds to position within the strain H16 genome. b c The primer was provided in the 5’RACE and 3’RACE KIT.