Table S1 Genotyping of the ZC-Lpa Cu F5 Population Using Three Methods

Table S1 Genotyping of the ZC-lpa × Cu F5 population using three methods Sample code DNA concentration Regular HRMb CAPS CADMAc (ng/µl)a 1 1832.1 W W W 2 1358.3 M M M 3 330.9 M W W 4 456.6 M W W 5 427.5 M M M 6 746.2 M W W 7 321.3 M M M 8 998.6 H H H 9 364.3 H M M 10 455.4 W W W 11 617.4 M H H 12 1042.7 H H H 13 917.0 H M M 14 1447.3 H H H 15 804.0 H H H 16 1079.0 M M M 17 265.9 H H H 18 762.9 H H H 19 887.9 H H H 20 1050.4 M M M 21 871.2 H H H 22 531.7 H H H 23 807.7 H H H 24 1282.7 H H H 25 946.8 H H H 26 333.0 H H H 27 353.9 H H H 28 1119.8 M W W 29 545.4 M W W 30 914.9 M W W 31 1064.4 M W W 32 629.3 H H H 33 452.7 M W W 34 1253.8 M M M 35 805.0 H H H 36 1223.4 H W W 37 633.6 M M M 38 487.7 M M M 39 355.3 M M M Table S1, continued Sample code DNA concentration Regular HRMb CAPS CADMA-HRMc (ng/µl)a 40 581.3 M M M 41 1205.3 M M M 42 859.7 M M M 43 1497.0 M M M 44 1723.9 W M M 45 1039.7 M M M 46 647.6 M M M 47 574.8 M M M 48 735.6 M M M 49 1300.5 M M M 50 340.0 W M M 51 616.3 M M M 52 503.2 M M M 53 271.4 H M M 54 624.3 M M M 55 911.7 M M M 56 754.1 M M M 57 498.4 M M M 58 612.9 M M M 59 498.4 M M M 60 1905.0 W W W 61 1269.9 M W W 62 1399.4 W W W 63 812.9 M W W 64 514.2 M W W 65 1062.6 W W W 66 1266.3 M W W 67 1423.6 W W W 68 611.8 M W W 69 1313.2 W W W 70 2134.2 M W W 71 520.7 M W W 72 1004.3 H H H

Seventy-two plants from the F5 population of ZC-lpa × Cu were genotyped using regular HRM, CADMA-HRM and CAPS analysis. H, M and W represent heterozygous, mutant and wild-type genotypes, respectively. The samples in red showed the inconsistence of the genotyping result between HRM and CAPS analysis a After DNA was extracted from leaves using the CTAB method, the DNA concentration was detected using Nano-drop b The DNA was pre-adjusted to 50 ng/µl and used for regular HRM analysis c The raw DNA was used directly for CADMA-HRM analysis