Lock Haven University of Pennsylvania

Syllabus approved by UCC: 9/23/10

Lock Haven University of Pennsylvania Lock Haven, Pennsylvania Department of Biological Sciences BIOL321

Marine Molecular Technology: Applications for Management and Forensics.

I. Introductory Information A. Department Name: Biological Sciences B. Department Catalog Number: BIOL321 (at Wallops as MS321) C. Course Title: Marine Molecular Technology: Applications for Management and Forensics. D. Semester Hours of Credit: 3 (2 lect/3 lab) E. Clock Hours Per Week: 5 F. Overlays: None G. Restrictions Upon Student Registration: Prerequisites are Principles of Biology I and II (BIOL106, BIOL107). Genetics (Biol202) is recommended. This class will be taught only at the Wallops Island Marine Station Campus during summer sessions. Students must see Dr. Thompson before registering. II. Description of the Course A. Catalog description: Students will gain an overview of modern molecular technology and how it can be applied to the management of marine organisms and the forensics field. The laboratory component will allow students to learn some of the most widely used techniques and instrumentation in the molecular field (may not be used in place of BIOL220 - DNA Methods in Biology or BIOL345 - Advanced DNA Methods).

B. Comprehensive description: (Optional) not needed III. Exposition A. Objectives: Upon completion of this course, students will be able to do the following: 1. Explain DNA replication, transcription and translation 2. Describe basic molecular techniques employed in the field of molecular biology such as PCR, restriction analysis and blotting techniques. 3. Understand the basics of bioinformatics and their applications.

1 Syllabus approved by UCC: 9/23/10

4. Explain how these techniques are applied to management of marine organisms and forensic studies in marine science. 5. Apply this biotechnology to the study of environmental impacts. 6. Understand the legal issues of such impacts on marine systems.

B. Activities and Requirements: Successful completion of this course will require the following: 1. Attendance and participation at lectures, laboratory sessions and field trips. 2. Reading assigned materials in the form of texts, handouts, web sites, and books on reserve. 3. Completion of laboratory requirements including basic sampling techniques of marine organisms. 4. Understanding and carrying out standard molecular techniques such as: a. Agarose gell electrophoresis b. DNA extraction c. Restriction analysis of DNA and its application in marine science. d. PCR techniques and PCR application in marine science. e. Protein extraction and Western blotting techniques 5. Independent project assignments.

C. Major Units and Time Allotted: Topic Contact Hours 1. Basic cellular biology 2.0 2. DNA replication & transcription 2.0 3. Translation 2.0 4. Restriction enzyme analysis and PCR 3.0 5. Southern and Western blotting techniques 3.0 6. Basic proteomics 2.5

7. Beginning bioinformatics 2.5 8. Biotech applications to management of marine organisms 2.0 9. Forensic biotech applications in marine sciences 3.0 10. Basics of genetic expression 2.0 11. Recombinant DNA technology & application to marine science 2.0 12. Environmental impact of biotechnology in marine science 2.0 13. Regulations and the law 2.0

Total contact hours in lecture 30

D. Materials and Bibliography: 1. Suggested textbooks: A current text such as the following is recommended.

Fingerman, M., and R. Nagabhushanam. 2000. Recent advances in marine biotechnology: Aquaculture-Fishes. Science Publishers.

2. Other materials: None

3. Bibliographic support:

Committee on Marine Biotechnology. 2002. Marine biotechnology in the twenty- first century: Problems, promise and products. National Academies Press.

Dawson, M. N., K. A. Raskoff and D. K. Jacobs. 1998. Field preservation of marine invertebrate tissue for DNA analyses. Molecular Marine Biology and Biotechnology. 7(2):145-152. Hauser, L. 2009. The molecular ecology of dogfish sharks. In: Biology and Management of Dogfish Sharks. American Fisheries Society. Heist, E. J. 2004. Genetics of sharks, skates and rays. In: Carrier, J. C., J. A. Musick and M. R. Heithaus 2004. Sharks and their relatives. CRC Press. Le Gal, Y. and H.O. Halvorson. 2010. New developments in marine biotechnology. Springer, USA. Parry, W. 2010. Dolphin snot offers less painful source of DNA. Retrieved Sept. 8, 2010 from World Wide Web - http://www.livescience.com/animals/DNA- collected-when-dolphins-exhale-100830.html Pfeiffer, C. J. 2002. Molecular and cell biology of marine mammals. Krieger Publishing. Shivji, M. S. 2010. DNA forensic applications in shark management and conservation. In: Carrier, J. C., J. A. Musick and M. R. Heithaus. 2010. Sharks and their relatives II. CRC Press. IV. Standards Grades will be assigned in accordance with University policy. They will be based on a combination of examinations, quizzes, web and writing assignments, attendance, and classroom participation.

V. Rationale and Impact: A. This course is intended to be taught during summer sessions at the Wallops Island Campus of the Marine Science Consortium. It will be an elective in biology. B. Students in the Marine Biology concentration are required to take six semester hours of electives in marine biology. This course will meet those requirements. Students in all other BS Biology concentrations and the BS Biology/Chemistry program will be able to take it as an elective in biology.

C. No other department will be affected.

VI. Cost and Staff Analysis: A. This class will be taught during summer sessions at the Wallops Island Campus of the Marine Science Consortium by faculty from various member universities. No new Lock Haven University faculty or equipment will be required.

B. This class will be taught by faculty from various members of the Marine Science Consortium as demand dictates.