PHS 398 (Rev. 9/04), Biographical Sketch Format Page s5

Curriculum Vitae Provide the following information for the key personnel and other significant contributors. Follow this format for each person. DO NOT EXCEED FOUR PAGES.

NAME POSITION TITLE Pillus, Lorraine Professor of Biology eRA COMMONS USER NAME lpillus EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) DEGREE INSTITUTION AND LOCATION YEAR(s) FIELD OF STUDY (if applicable) Brown University, Providence, RI B.A. 1980 Biology Massachusetts Institute of Technology, Ph.D. 1986 Biology Cambridge, MA University of California, Berkeley, CA Postdoctoral 1987-1991 Genetics/Biochemistry

Research and Professional Experience:

1980-1986 Graduate research in the laboratory of Dr. Frank Solomon, Department of Biology & Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 1987-1991 Postdoctoral research in the laboratory of Dr. Jasper Rine, Department of Molecular Cell Biology, Genetics Division, University of California, Berkeley, CA 1991-1998 Assistant Professor, Department of Molec, Cell. & Dev. Biol., Univ. of Colorado, Boulder, CO 1998 Associate Professor, Department of Molec, Cell. & Dev. Biol., Univ. of Colorado, Boulder, CO 1998- 2002 Associate Professor, Molecular Biology, Division of Biology, Univ. of California, San Diego 2002-Present Professor, Molecular Biology, Division of Biological Sciences, Univ. of California, San Diego

Fellowships, Awards, Honors:

1984-1985: Johnson & Johnson Graduate Fellow, M.I.T. 1987-1990: John D. and Catherine T. MacArthur Foundation Fellow, Life Sciences Research Foundation 1994: Mortar Board Honor Society Teaching Excellence Award 1992-1996: Pew Scholar in the Biomedical Sciences, The Pew Charitable Trusts 1992-1997: National Science Foundation New Young Investigator 1997: American Society for Cell Biology, Women in Cell Biology Junior Career Recognition Award 2003-2004: Member, Science & Society Institute of the Pew Charitable Trusts 2005: UCSD Skaggs School of Pharmacy & Pharmaceutical Sciences Teaching Excellence Award

Professional Societies and Activities:

American Association for the Advancement of Science American Society for Cell Biology, Publications Committee, Program Committee American Society for Human Genetics American Society for Microbiology, Division X, Alternate Councilor 1999-2001 Genetics Society of America, Yeast Genetics Meeting Program Committee, 1996-2000 The Wellcome Trust, International Fellowship Review, 2000 Editorial Reviewer for BioEssays, Cell, Genes & Development, Genetics, J. Cell Biology, Molecular and Cellular Biology, Nature Genetics, PLoS, Science, TIBS and Proc. Natl. Acad. Sci., USA National Science Foundation: Ad hoc special projects review (1992, 1994, 1998, 1999, 2000) Member, Univ. of Colorado Health Sciences Center Cancer Center, 1996-1998 Member, AIDS-Related Malignancies Focus Group, Univ. Colorado Health Science Center, 1996-1998 National Advisory Board, Genetic Education for Native Americans, 1998-2004 Associate Editor, Genetics 2001-present Editorial Board, Molecular Cell, 2001 - present Member, UCSD Center for Molecular Genetics and UCSD Moores Cancer Center National Cancer Institute Site Review Panel, Genetics Division, 2000, 2004 Co-organizer, FASEB Conference on Chromatin and Transcription, 2001 Co-Organizer, Keystone Conference on Chromatin Structure and Activity, 2002 Keystone Symposia Scientific Advisory Board, 2003-present University of California Cancer Research Coordinating Committee, 2005- present

Selected Publications:

Pillus, L. and F. Solomon (1986) Components of microtubular structures in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci., USA 83:2468-2472. Bond, J.F., J.L. Fridovich-Keil, L. Pillus, R.C. Mulligan and F. Solomon (1986) A chicken-yeast chimeric-tubulin protein incorporated into mouse microtubules in vivo. Cell 44:461-468. Schatz, P.J., L. Pillus, P. Grisafi, F. Solomon and D. Botstein (1986) Two functional-tubulin genes of the yeast Saccharomyces cerevisiae encode divergent proteins. Mol. Cell Biol. 6:3711-3721. Pillus, L. and F. Solomon (1986) Overview: Why the yeast cytoskeleton? In: Yeast Cell Biology (J. Hicks, ed.), Alan R. Liss, New York, pp.3-12. Pillus, L. and J. Rine (1989) Epigenetic inheritance of transcriptional states in S. cerevisiae. Cell 59:637-647. Pillus, L. (1991) Any which way but loose—determining a transcription state in yeast. BioEssays 13:303-304. Pillus, L. (1992) An acquired state: epigenetic mechanisms in transcription. Curr. Op. Cell Biol. 4:453-458. Palladino, F., T. Laroche, E. Gilson, A. Axelrod, L. Pillus and S.M. Gasser (1993) SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres. Cell 75:531-542. Palladino, F., T. Larouche, E. Gilson, L. Pillus and S. Gasser (1994) Sir3 and Sir4 proteins function in telomeric positioning and integrity. Cold Spring Harbor Symposium on Quantitative Biology LVIII:733-746. Rivier, D. and L. Pillus (1994) Silencing speaks up. Cell 76:963-966. Pillus, L. and M. Grunstein (1995) Chromatin structure and epigenetic regulation in yeast. In: Chromatin Structure & Gene Expression (S.C.R. Elgin, ed.). Oxford: Oxford University Press, pp. 123-146. Brachmann,C., J.Sherman, S.Devine, E.Cameron, L.Pillus and J.Boeke (1995) The SIR2 gene family, conserved from bacteria to humans, functions in silencing, cell cycle progression and chromosome stability Genes & Development 9:2888-2902. Stone, E.M. and L. Pillus (1996) Activation of a MAP kinase cascade leads to Sir3p hyperphosphorylation and strengthens transcriptional silencing. J. Cell Biol. 135:571-583. Reifsnyder, C., J. Lowell, A. Clarke and L. Pillus (1996) Yeast silencing genes SAS2 and SAS3 and human genes associated with acute myeloid leukemia and with HIV-1 Tat interactions are homologous and share similarities with acetyltransferases. Nature Genet. 14:42-49. Sherman, J.M. and L. Pillus (1997) An uncertain silence. Trends Genet. 13:308-313. Nislow, C., E. Ray and L. Pillus (1997) SET1, a yeast member of the Trithorax family, functions in transcriptional regulation and diverse cellular processes. Mol. Biol. Cell 8:2421-2436. Stone,E.M. and Pillus,L. (1998) Silent chromatin in yeast: an orchestrated medley featuring Sir3p. BioEssays 20:30-40. Lowell, J.E. and L. Pillus (1998) Telomere tales: chromatin, telomerase and telomere function in Saccharomyces cerevisiae. Cell. Mol. Life Sci. 54:32-49. Smith, J.S., C.B. Brachmann, L. Pillus and J.D. Boeke (1998) Distribution of a limited Sir2 protein pool regulates the strength of yeast rDNA silencing and is modulated by Sir4p. Genetics 149:1205-1219. Clarke, A.S., J. Lowell, S. Jacobson and L. Pillus (1999) Esa1p is an essential histone acetyltransferase required for cell cycle progression. Molec. Cell. Biol. 19:2515-2526. Allard, S., R. T. Utley, J. Savard, A. Clarke, P. Grant, C.J .Brandl, L. Pillus, J.L. Workman, and Côté,J (1999) NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p. EMBO J. 18:5108-5119. Jacobson,S. &L.Pillus(1999) Modifying chromatin and concepts of cancer. Curr.Opin. in Genetics and Devel. 9:175-184 Garcia, S.N. and L. Pillus (1999) Net results of nucleolar dynamics. Cell 97: 825-828. Sherman, J., E.M. Stone, L. Freeman-Cook, C. Brachmann, J. Boeke and L. Pillus (1999). The conserved core of a human SIR2 homologue functions in yeast silencing. Mol. Biol. Cell 10:3045-3059. Freeman-Cook,L.L., J.M.Sherman, C.B.Brachmann, R.Allshire, J.D.Boeke & L.Pillus (1999) The Schizosaccharomyces pombe hst4+ gene is a SIR2 homologue with silencing and centromeric functions. Mol. Biol. Cell 10:3171-3186. Stone, E.M., C.M. Reifsnyder, M. McVey, B.M. Gazo, and L. Pillus (2000) Two classes of sir3 mutants enhance the sir1 mutant mating defect and abolish telomeric silencing in S. cerevisiae. Genetics 155: 509-522. Stone, E.M., P. Heun, T. LaRoche, L. Pillus and S.M. Gasser (2000) MAP kinase signaling induces nuclear reorganization in budding yeast. Current Biology 10: 373-382. Landry, J, A. Sutton,, S. Tafrov, R. Heller, J. Stebbins, L. Pillus and R. Sternglanz (2000) The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases. Proc. Natl. Acad. Sci., USA 97:5807-5811. Freeman-Cook, L., R. Kamakaka & L. Pillus (2000) Chromatin contributions to epigenetic transcriptional states in yeast. In: Chromatin Structure & Gene Expression (S.C.R.Elgin & J.Workman,eds).Oxford:Oxford University Press pp.203-227. Dutnall, R.D. and L. Pillus (2001) Deciphering NAD-dependent deacetylases. Cell 105:161-164. Howe, L., Auston, D., Grant, P., John, S., Cook, R.G., Workman, J.L., and L. Pillus (2001) Histone H3 specific acetyltransferases are essential for cell cycle progression Genes & Development 15: 3144-3154. Garcia, S.N. and Pillus, L. (2002) A unique class of conditional sir2 mutants display distinct silencing defects in Saccharomyces cerevisiae. Genetics 162:721-736. Parsons, X-J., Garcia, S.N., l. Pillus and J.T. Kadonaga (2003) Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex. Proc. Natl. Acad. Sci., USA 100:1609-1614. Clements, A., A.N. Poux, W.-S. Lo, L. Pillus, S.L. Berger, R. Marmonstein (2003) Structural basis for histone and phospo-histone binding by the GCN5 histone acetyltransferase. Mol. Cell 12: 461-473. Lowell, J.E., A.I. Roughton, V. Lundblad and L. Pillus (2003) Telomerase-independent proliferation is influenced by cell-type in Saccharomyces cerevisiae. Genetics 164: 909-921. Henry, K.W., Wyce, A., Lo, W.-S., Duggan, L.J., Emre, T., Kao, C., Pillus, L., Shilatifard, A., Osley, M.A., and S.L. Berger (2003) Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Genes & Development 17:2648-2663. Murphy, G.A., E, J. Spedale, S. T. Powell, L. Pillus, S. C. Schultz, and L. Chen (2003) The Sir4 C-terminal coiled-coil is required for telomeric and mating type silencing in Saccharomyces cerevisiae J. Mol. Biol. 334:769-780. Jacobson, S., Laurenson, P. and L. Pillus (2004) Functional analysis of chromatin modification in yeast. Methods in Enzymology 377: 3-55 (C. Wu and C.D. Allis, eds.). Jacobson, S., Laurenson, P. and L. Pillus (2004) Molecular requirements for gene expression mediated by targeted histone acetyltransferases. Mol. Cell. Biol. 24:6029-6039. Freeman-Cook, L., E. B. Gomez, E. J. Spedale, J. Marlett, S. L. Forsburg, L. Pillus and P. Laurenson (2005) Conserved locus-specific silencing functions of S. pombe sir2+. Genetics 169:1243-1260. Lo, W.-S., E.R. Gamache, K.W. Henry, D. Yang, L. Pillus, S.L. Berger (2005) Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms. EMBO J. 24: 957-1008. Pillus. L. and J. Rine (2005) SIR1 and the origin of epigenetic states in Saccharomyces cerevisiae. Cold Spring Harbor Symposium on Quantitative Biology LXIX: 259-265. Wirén, M., Silverstein, R. A., Sinha, I., Walfridsson, J., Lee, H., Laurenson, P., Pillus, L., Robyr, D., Grunstein, M., and Ekwall, K. (2005) Genome wide analysis of nucleosome density, histone acetylation, and HDAC function in fission yeast. EMBO J. 24:2906-2918. Kennedy, E.J. L. Pillus, and and G. Ghosh (2005) Pho5p and newly identified nucleotide pyrophosphatases/phosphodiesterases regulate extracellular nucleotide phosphate metabolism in Saccharomyces cerevisiae. Eukaryotic Cell 4:1892-1902. Clarke, A.S., Samal, E., and Pillus, L. (2006) Distinct roles for the essential MYST family HAT Esa1p in transcriptional silencing. Mol. Biol. Cell 17: 1744-1757. Wilson, J.M., Le, V.Q., Zimmerman, C., Marmorstein, R., and Pillus L (2006) Nuclear export modulates the cytoplasmic Sir2 homologue Hst2 EMBO Reports 7:1247-1251 Ruault, M. and Pillus, L. (2006) Chromatin modifiying enzymes are essential when the Saccharomyces cerevisiae morphogenesis checkpoint is constitutively activated Genetics 174:1135-1149 Lafon A, Chang CS, Scott EM, Jacobson SJ, and Pillus L. (2007) MYST opportunities for growth control: yeast genes illuminate human cancer gene functions. Oncogene. 26:5373-5384. Allis C.D., Berger S.L., Cote J., Dent S., Jenuwein T., Kouzarides T., Pillus L., Reinberg D., Shi Y., Sheikhattar R., Shilatifard A., Workman J., and Zhang Y. (2007) New nomenclature for chromatin-modifying enzymes. Cell. 131:633-636. Kennedy, E.J., Ghosh G., and Pillus, L. (2008) Identification of functionally distinct regions that mediate biological activity of the Protein Kinase A homolog TPK2. Journal of Biological Chemistry. 283: 1084- 1093. Darst, R.P., Garcia, S.N., Krick, M., and Pillus, L. (2007) Slx5p promotes transcriptional silencing and is required for robust growth in the absence of Sir2p. Molecular and Cellular Biology. 28:1361-1372. Pillus L (2008) MYSTs Mark Chromatin for Chromosomal Functions. Current Opinion in Cell Biology (in press). Kennedy E.J., Yang J., Pillus L., Taylor S., and Ghosh G. Identifying critical non-catalytic residues that modulate PKA activity. (Submitted) Jacobson S; Pillus L The SAGA subunit Ada2p functions in transcriptional silencing. (Submitted)