Customer Information s1

Protein Expression and Purification Service Quotation Request Form

Instructions Please answer all of the following questions and send the completed form to [email protected] if you choose bacterial, yeast, and/or baculovirus/insect cell expression system(s); Send the completed form to [email protected] if you choose mammalian cell expression system. Our service representative will contact you with a quote shortly. Customer Information If you have registered an account with GenScript, you can just identify yourself by giving us your name and email address or Account No. Name: Account No.: Phone: Organization: Shipping address: (Necessary to determine shipping cost) Email address:

Protein Expression Protein name: Species: MW: Target protein: Protein sequence: (crucial for evaluation) or accession number: Note: Please enter the protein sequence if mutants are included in your protein sequence. Gene synthesis with codon optimization (Gene synthesis starting from $0.39/bp) (Please enter your gene sequence: ) Gene synthesis without codon optimization (Gene synthesis starting from $0.39/bp) (Please enter your gene sequence: ) Your supplied template DNA Starting material: (Please enter your gene sequence: , vector name: and attach vector map (e.g. NTI file) Your supplied expression ready construct (Please give a brief description of your expression construct: ) Others (Please specify): Please indicate the preferred expression host system: Bacteria Yeast Baculovirus/insect cell Mammalian cell If mammalian cell expression system is preferred, please select preferred host cell line: HEK293 CHO Others (Please specify): No preference If mammalian cell expression system is preferred, please choose whether GLP compliance service is preferred: Yes No Notes: If you DO NOT have preference, HEK293 or its derivatives will be used as default cell line in our platform. Expression system: Has the gene been expressed in any host before? Yes No If YES, please indicate the host: Bacteria Yeast Baculovirus/insect cell Mammalian cell Others (Please specify): Yield: Solubility: Protocol: Other information: Notes: The above information is crucial for us to evaluate your project. Do you have any preferred secretion signal sequence for expressing your protein of interest? Its own signal peptide No preference Others (Please specify): Secretion signal sequence: (For insect Please specify your preferred expression format: cell and mammalian cell Secretory expression only (GenScript will only purify the protein from the medium) expression systems) Protein purification from the cell lysate if very little protein can be obtained from the medium (Extra fee is charged) Expression evaluations on both secretory expression with the signal peptide and intracellular expression without the signal peptide (Recommended) Expression scale: Amount of protein: mg or Volume of culture required: liter(s) Membrane-bound Secreted A protease Other enzyme Cytoplasmic protein IgG antibody Toxic to E.coli Protein properties: Other features that may cause difficulty in either expression or purification (Please specify): Application(s) of your High-throughput screening (HTS) Activity assays NMR X-ray protein: (Please select crystallography Antigen in vivo studies all that apply) Protein-protein interactions Others (Please specify): Comments:

Protein Purification Product requirements: Purity: % Endotoxin Limit: Concentration: Do you want GenScript to recommend a purification system for purifying your protein of interest? Yes No, I want to choose my own purification system (Please specify): Purification method: Do you need GenScript to follow any specific expression and/or purification protocol? Yes (Please attach your protocol: ) No Note: Your protocol will be very helpful to increase success rate and shorten the delivery time. Do you want GenScript to select 1 or 2 tags (6xHis, GST, or MBP) to facilitate purification? Yes No, I want to choose my own tag(s) (Please specify):

Do you want GenScript to select additional 1 or 2 tags (Trx, TF, Dsb, or Nus) to increase solubility and/or expression level? (Recommended—can greatly improve yield; the tag(s) will be Tag(s) selection: added to N-terminus) Yes No, I want to choose my own tag(s) (Please specify):

Do you want GenScript to add the tag(s) at the N-terminus? Yes No, I have designated place(s) to add the tag(s) (Please specify): Note: If internal tag(s) is/are involved, please designate the position number(s) that corresponds to the sequence of which the accession number you have indicated above. Do you want GenScript to add a protease cleavage site between the tags and the target protein? Yes No If YES, please specify the protease you want to add: GenScript’s choice Enterokinase PreScission rTev Thrombin Tag(s) removal: Note: An extra of 4-6 aa will be left after protease cleavage of C-terminal tag, therefore, we DO NOT recommend the introduction of protease cleavage site between the target protein and the C- terminal tag.

Do you want GenScript to remove tag(s) in the purification step? Yes No Small tag (e.g. His) can be retained after purification Note: The recovery rate varies with the characteristics of the protein. If the expressed protein is insoluble, do you want GenScript to attempt the refolding? Refolding: Yes No Note: GenScript will NOT charge extra fee for conventional refolding. Protein SDS-PAGE IEF ELISA HPLC Endotoxin characterization: N-terminal sequence MALDI-TOF Others (Please specify): Special requirements: (information on buffers or handling, etc.) Comments:

Project Information Nature of inquiry: Pricing estimation Quote for ordering For grant application purpose Immediately Within one month Within three months Order initiation: Within six months