Participant Demographics

Supplementary Material Marusak et al., 2014

Data Supplement for Marusak, Martin, Etkin, and Thomason, Childhood trauma exposure disrupts the automatic regulation of emotional processing, Neuropsychopharmacology

Methods

The present sample of low-income, urban, minority youth was chosen based on high sociodemographic risk. Prior research shows that minority, urban residents are nearly two times more likely to develop emotional psychopathology following trauma exposure (Gillespie et al, 2009; Goldmann et al, 2011; Kessler et al, 1995). In addition, trauma frequency is extreme among African Americans living in impoverished areas

(nearly 90%; Gillespie et al, 2009). Despite this population’s apparent increased susceptibility to stress, little research has examined trauma and its neural correlates in high-risk, urban residents.

Reward Sensitivity

To examine individual differences in RS, we focused on the Behavioral Activation

System (BAS) component of the BIS/BAS scales (Carver and White, 1994). The BAS is comprised of three subscales: drive, fun seeking, and reward responsiveness. These subscales are intercorrelated (r’s > 0.3, p’s ≤ 0.05 in the present sample), but relate to discrete aspects of reward behavior. BASdrive captures the strength with which reward outcome guides subsequent behavior, and has been used as a measure of trait reward- seeking (Hickey et al, 2010). BASfun seeking is akin to trait novelty-seeking, whereas reward responsiveness (BASrr) indexes the degree to which a person derives pleasure from reward. We tested each of the BAS subdimensions, as well as a combination of the three (Garner et al, 2012) using z-scores to form an overall index of RS.

1 Experimental paradigm

The task consisted of 163 presentations of happy or fearful facial expression photographs, overlaid with the words “FEAR” or “HAPPY” to create emotionally congruent and incongruent stimuli (see Fig.1). Participants were instructed to indicate the facial affect with a button press response, while trying to ignore the task-irrelevant word stimuli. Stimuli were presented for 1,000 ms, with a varying interstimulus interval of

2,000-4,000 ms (mean = 3,000 ms), in a pseudorandom order, counterbalanced across trial types for expression, word, response button, and gender. Participants were given a practice task before entering the scanner. Stimuli were presented with EPrime software v.2.0 (Psychology Software Tools, Inc., Pittsburgh, PA) during functional MRI (fMRI) scanning and displayed on a back-projection screen that was viewed by the participants via a mirror attached to the head coil. Task duration was 12:46.

Imaging Data Analysis

All fMRI data were processed using SPM8 software (Statistical Parametric

Mapping; http://www.fil.ion.ucl.ac.uk/spm/) implemented in MATLAB (MathWorks, Inc.,

Natick, MA). The first four image volumes were excluded from analysis to allow for signal equilibration effects. Preprocessing steps included: (i) image realignment, (ii) spatial transformation to the Montreal Neurological Institute (MNI) template using the participant-specific transformation parameters created by fitting mean functional images to the single reference EPI standard template (in SPM). Data were not resampled during normalization, thus retained the native resolution (3.44 x 3.44 x 4 mm) for subsequent analysis. (iii) Images were then spatially smoothed with a Gaussian kernel of 6 mm full width at half maximum.

A 128-second temporal high-pass filter was applied to the data, and temporal autocorrelation was estimated using a first-order autoregressive model. Two independent participant-level models were created in the context of a general linear

2 Supplementary Material Marusak et al., 2014 model to examine effects of (1) conflict and (2) conflict regulation. In the first model, separate regressors for the stimulus events (convolved with a canonical hemodynamic response function) were created for incongruent (I) and congruent (C) trials. 82 experimental trials were incongruent, and 81 were congruent. For the second model, trial types were broken down based on the preceding trial type: regressors were created for postcongruent incongruent trials (cI), postincongruent incongruent trials (iI), postcongruent congruent trials (cC), and postincongruent congruent trials (iC). There were 38 cI trials, 44 cC trials, 37 iC trials, and 44 iI trials. All participant-level models included regressors of no interest corresponding to the six motion parameters, and modeled error and posterror trials separately. Participant-level contrasts isolated (1) conflict-related neural activity by comparing I-C trials, and (2) conflict regulation by comparing iI-cI trials.

3 Table S1. Behavioral effects of task congruency. ACC (%) RT (ms) Trial type M SD M SD Congruent (C) 86.91 10.63 859.39 171.18 Incongruent (I) 77.99 15.09 898.19 186.96 A b b r e v i a t i o n s : A C C , a c c u r a c y ; R T , r e a c t i o n t i m e ; m e a n , M ; s t a n d a r d d e v i a t i o n , S D .

4 Supplementary Material Marusak et al., 2014

Table S2. Group Differences in Whole-Brain Activity During the Emotional Conflict Task. peak Contrast Brain Region BA x y z volume (voxels) T-score Conflict (I-C) trauma > comparison L cerebellum n/a -36 -76 -24 18 3.08 R midbrain n/a 14 -12 -8 59 3.21 L midbrain n/a -18 -16 -12 57 3.35 L middle occipital gyrus 37 -38 -70 8 338 3.52 R middle temporal gyrus 41 38 -48 4 41 3.5 R primary visual cortex 19 26 -64 4 53 3.2 R middle occipital gyrus 19 38 -70 6 19 3.52 R middle occipital gyrus 19 44 -80 10 116 3.44 L middle occipital gyrus 19 -26 -82 20 243 3.39 L cuneus 19 -2 -92 26 40 3.29 R superior occipital gyrus 19 20 -90 30 59 3.29 L middle frontal gyrus 6 -28 -12 42 32 3.16 L precentral gyrus 6 -49 -4 48 24 3.18 L paracentral lobule 7 14 -42 54 25 3.09 comparison > trauma no voxels survived at this threshold Conflict regulation (iI-cI) trauma > comparison L lingual gyrus 18 -20 -92 -18 265 3.68 R lingual gyrus 17 6 -100 2 851 5.19 L superior occipital lobe 17 -22 -82 6 15 3.03 L superior temporal lobe 40 -54 -30 18 37 3.4 L superior occipital lobe 19 -26 -88 22 42 3.03 L middle temporal gyrus 39 -30 -62 24 22 3.04 L middle occipital gyrus ? -30 -74 32 35 3.18 R cuneus 19 6 -86 32 13 3.01 L middle frontal gyrus 9 -46 30 38 26 3.42 R middle frontal gyrus 9 48 32 40 14 3.25 R precuneus 7 6 -84 42 12 3.04 R precentral gyrus 6 38 -8 56 76 3.91 L middle frontal gyrus 6 -44 6 56 42 3.84 L superior frontal gyrus 6 -26 -4 68 168 4.05 comparison > trauma no voxels survived at this threshold W h o l e - b r a i n r e s u l t s p r o v i d e d a t p < 0 . 0 0 5 u n c o r r e c t e d , c l u s t e r m i n i m u m = 1 0 v o x e l s . A b b r e v i a t i o n s : B A , B r o d m a n n ' s A r e a ; L , l e f t ; R , r i g h t ; I - C , i n c o n g r u e n t m i n u s c o n g r u e n t ; i I - c I , p o s t i n c o n g r u e n t i n c o n g r u e n t m i n u s p o s t c o n g r u e n t i n c o n g r u e n t . C o o r d i n a t e s g i v e n i n M N I c o n v e n t i o n

5 Reference List for Supplementary Material

Carver CS, White TL (1994). Behavioral inhibition, behavioral activation, and affective responses to impending reward and punishment: The BIS/BAS Scales. Journal of Personality and Social Psychology 67(2): 319-333.

Garner KG, Dux PE, Wagner J, Cummins TD, Chambers CD, Bellgrove MA (2012). Attentional asymmetries in a visual orienting task are related to temperament. Cognition & emotion 26(8): 1508-1515.

Gillespie CF, Bradley B, Mercer K, Smith AK, Conneely K, Gapen M, et al (2009). Trauma exposure and stress-related disorders in inner city primary care patients. General hospital psychiatry 31(6): 505-514.

Goldmann E, Aiello A, Uddin M, Delva J, Koenen K, Gant LM, et al (2011). Pervasive exposure to violence and posttraumatic stress disorder in a predominantly African American Urban Community: the Detroit Neighborhood Health Study. Journal of traumatic stress 24(6): 747-751.

Hickey C, Chelazzi L, Theeuwes J (2010). Reward guides vision when it's your thing: trait reward-seeking in reward-mediated visual priming. PloS one 5(11): e14087.

Kessler RC, Sonnega A, Bromet E, Hughes M, Nelson CB (1995). Posttraumatic stress disorder in the National Comorbidity Survey. Archives of general psychiatry 52(12): 1048-1060.