Journal: Plant Cell Reports

1An expression analysis of 57 transcription factors derived

2from ESTs of developing seeds in Maize (Zea mays)

3Journal: Plant Cell Reports

4Guifeng Wang*, Hui Wang*, Jia Zhu, Jing Zhang, Xiaowei Zhang, Fei Wang, Yuanping Tang,

5Bing Mei, Zhengkai Xu, Rentao Song§

71Shanghai Key Laboratory of Bio-Energy Crops, Nanchen Road, Shanghai, P.R. China

82School of Life Sciences, Shanghai University, Nanchen Road, Shanghai, P.R. China

10*These authors contributed equally to this work

11§Corresponding author ([email protected])

13Supplementary Tables

14Supplementary Table 1 Primers used for semi-quantitative RT-PCR in this study

Name Primer Sequence (5’----3’) Product (bp)

ZmZF1 Sense AGAGGACTGCCACTAAGCTTTT 472 Antisence CGGGGATTTTCTTCATCATATT ZmZF2 Sense GCTGGCAGCATGACTGTTTA 416 Antisence ACGGAGTTCCCTATGCCATA ZmZF3 Sense GGGCTCGAGTTCACATTCTC 442 Antisence CGGATAAGTTTCTGGTTGCA ZmZF4 Sense ATGGGTGGCTGTTGTTGCTG 415 Antisence AGCGGGGATTTTCTTCATCG ZmZF5 Sense GGAGAGAGGGAGAAGGGAGA 410 Antisence GGAACTTATGCTGACAGGGC ZmZF6 Sense CAAAATCTCATGACCAGCCG 409 Antisence TCGTGGAATTGGCTGATTTT ZmZF7 Sense TGGCAGCGTCTTGTGATCAT 664 Antisence CATCCTCTGAAGTTTTGCTGGT ZmZF8 Sense GCGACGAAGACCCTGGCTAT 465 Antisence ATTCTGCCGCTCTGCGACTA ZmZF9 Sense CAGATCATGCACCCGTTACA 447 Antisence GCCATTTTCATGTCGAGGTT ZmZF10 Sense GCCAAGCACTTCAAGTGCCA 575 Antisence GAGGGGCAACAGGTGAAGGT ZmZF11 Sense GAGACCCCCGCAAGTACCACTA 408 Antisence TTGGAACATTTGGCTGCTGC ZmZF12 Sense CCTTCAAATTATGCGCCGAT 409 Antisence TGCAGATGCCGTAACGAGAA ZmZF13 Sense GGGCTGGTGGGATTTGTTTA 455 Antisence CGCGAACTTTTGATGAGGCT ZmZF14 Sense GCCTTCGTCACGTGGTTCTC 427 Antisence CTTGCACACTGCACCGAATT ZmZF15 Sense GCGATTCACTCCAACTAGGT 484 Antisence GCTTTGGGGGTAGTACCTTA ZmZF16 Sense ACCTGCTGTGAAGACTCGCA 424 Antisence CAACTCTGTGACCATTGCGC ZmZF17 Sense GCAGTCTAAGGCACGGGAGA 503 Antisence GGCCCACCGTAGAAGATCCT ZmbZIP1 Sense CAGGCATTACAGTCAGAAGGG 626 Antisence CGGGGGTAGTAACAGATTAGAAC ZmbZIP2 Sense AGAACGCAAGGGGTGCTCGA 527 Antisence GCCGGGGACATTCTCAAGCA ZmbZIP3 Sense TAACCACCTCAGCCAATC 461 Antisence CCCTAGTAAATCCAACCCT ZmbZIP4 Sense CAAGTCCACAGGGTCATC 774 Antisence GAGCCAACTCCTCACATT ZmbZIP5 Sense TGTGCTTAACCCTATGATGC 382 Antisence ATTTTCTGAGGTCAAGCCAT ZmbZIP6 Sense GGACCATGACTCCAAGTG 508 Antisence CGTATCCTCCTAAACCCA ZmbZIP7 Sense TCTCCGTGATAACCAACT 630 Antisence CTCTTCTCGGTGACTACAT ZmbZIP8 Sense GATTCTTTTGGACTCTGCTGG 603 Antisence GCTGAGATGTTGCCTGGTAA ZmbZIP9 Sense CTATTCCAAGGGAGACAACT 381 Antisence GGTTCAGATCCCTACGAGTT ZmbZIP10 Sense CTGGGAGTGAACCAACAA 654 Antisence TCCCGAGTGAATAACAGC ZmbZIP11 Sense CCATGGTCCCGGCTTCTAAT 501 Antisence CCCAAGCCACAAGAAGCACC ZmbZIP12 Sense CGAAGTCCAATAACGAGCAT 418 Antisence AAGGAGGAAGCAGTTCAAGG ZmbZIP13 Sense GTTTATGGGCACCCTGTC 610 Antisence GATGCTCCAAACGACCTG ZmAP2-1 Sense AAGGCAGAAAGACGGGAGAT 481 Antisence GCTTCGCTAACATTGCAAAA ZmAP2-2 Sense TGGGAACATTTGACACTCAAGA 491 Antisence ACCATCCTTGCTTCCTTCGT ZmAP2-3 Sense ATCGCAGTGAAGAAAATGGTT 530 Antisence TCGTCATAAACAGGAATCTCATC ZmMADS1 Sense ACCAGCAGGAGTCCTCCAAG 406 Antisence TGGAGGGTTGTAGGTTGCAG ZmMADS2 Sense TCACTGACCATAAAAGAACTCCA 431 Antisence CAAAACTACACTGGGAAGACCTC ZmMADS3 Sense AGAAAGACACAACTTATGATGGAGC 374 Antisence GGATGGATGATTAACAGAGCAAC ZmMADS4 Sense GTCGGCCTTGTCGTCTTCTC 464 Antisence TCAGACGACTGCCCTTCCTC ZmMADS5 Sense TGCTTGGTGAAGATCTTGAGTC 319 Antisence CCTATCTGAAGGGTGGGTTC ZmMADS6 Sense GCCTTTACGAGTACGCCAAC 521 Antisence GGGGAAGAAGCACTTCATGT ZmHD1 Sense TCAGGTGGACAGTGAGAGCA 522 Antisence GAGGGTCTTTTCTGGAGCAA ZmHD2 Sense GACCACGAGCTCAAGGAGAT 466 Antisence TCAACCTCAGCTCAGCAGATT ZmHD3 Sense GTCTTGCGAGGNTACATAATAAAG 555 Antisence AAGGAGAGTAGTGCCTGACAGTAT ZmbHLH1 Sense CGGTGTGTTGATTGTGAGCA 644 Antisence CCACTGGAGACATCACACGAA ZmbHLH2 Sense ATGCAGTGTGCAATATCCCTAC 501 Antisence TGTCTTCCGAGTCTAGTGCG ZmbHLH3 Sense ATGCTTGACGAGGCCATTGA 501 Antisence TGTGAGCAACCATCAAACTGAT ZmbHLH4 Sense GGATTTGAGCAGTCTAATTTGG 473 Antisence GGGATTGGAGCACTTTTGTT ZmbHLH5 Sense CAGAGTAGGATGATGTATTCCCC 374 Antisence ACGTAGTCCTCTCTCTTGGCTC ZmbHLH6 Sense GAGTAAGGGCTCGTCGTGGA 515 Antisence ATGGGAGCAGCAGCATGTCT ZmbHLH7 Sense AAAATGAGGAGAGACAAGCTGA 462 Antisence GCCAGAGCTTGGATTTTGAG ZmbHLH8 Sense CAAGCCTCAAGAGTTCATGGA 434 Antisence GCAAGTTCTGCATCTGGCTC ZmMYB1 Sense TCAGTGAAATCTGAGTGTGCATC 561 Antisence CTGAAAAGCATGTTGCATGTT ZmMYB2 Sense AGGATTATATTCGTAGTTCTGTCCC 576 Antisence CACTCGAACCTAACTGAATATCAGT ZmMYB3 Sense CCTGTTTCCAAGGACAGCGT 463 Antisence CTGAAGCCCAACCATCCATT ZmMYB4 Sense TTGGCACCACAAACGACAAA 542 Antisence CCCCAGCATTCTGATGCATC ZmMYB5 Sense GCGAAAAAGCTACTCCTAAGGG 481 Antisence TCAGACGAACGTTCTCCCAG ZmMYB6 Sense GAACTTCGAGGAGATGGGAG 476 Antisence GCTCCGGAAATTCTCTCTCA ZmMYB7 Sense CGAACGCTTCGAGTGTATTTG 554 Antisence CGTTGCTGACCTATTAGGTTCTACT cGAPDH Sense GTCACCGTCTTTGGCATCAG 511 Antisence CAACAACCGAGACATCCACA 1