OIE Reference Laboratory Reports s1

OIE Reference Laboratory Reports Activities in 2011

Name of disease (or topic) for Channel catfish virus which you are a designated OIE Reference Laboratory:

Address of laboratory: Fish Diagnostic Laboratory, College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, Mississippi State, Mississippi, UNITED STATES OF AMERICA 39762

Tel.: +1-662-325-1202

Fax: +1-662-325-1031

e-mail address: [email protected]

website:

Name (including Title and Dr Larry A. Hanson, Professor Position) of Head of Laboratory (Responsible Official):

Name(including Title and Dr Larry A. Hanson, Professor Position) of OIE Reference Expert:

Name (including Title and Position) of writer of this report

(if different from above):

Annual reports of OIE Reference Centres, 2011 1 Channel catfish virus

Part I: Summary of general activities related to the disease

1. Test(s) in use/or available for the specified disease/topic at your laboratory

Surveillance/ Disease Test For Specificity Total research diagnosis

Cell culture Virus NA 20 21 41

Serum Antibody species 0 0 0 neutralization

PCR Viral DNA species 30 16 46

2. Production and distribution of diagnostic reagents

We submitted the mycoplasma free CCO cell line to the American Type Culture Collection (ATCC) in 2003. This and the type CCV isolate are available from ATCC. CCV DNA and infected cell lysates are available for positive controls for PCR and antigen based tests. Neutralizing monoclonal antibody is available. There were no requests for these reagents in 2011.

Part II: Activities specifically related to the mandate of OIE Reference Laboratories

3. International harmonisation and standardisation of methods for diagnostic testing or the production and testing of vaccines

a) Establishment and maintenance of a network with other OIE Reference Laboratories designated for the same pathogen or disease and organisation of regular inter-laboratory proficiency testing to ensure comparability of results

b) Organisation of inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results

4. Preparation and supply of international reference standards for diagnostic tests or vaccines

We submitted the mycoplasma free CCO cell line to the American Type Culture Collection (ATCC) in 2003. This and the type CCV isolate are available from ATCC. CCV DNA and infected cell lysates are available for positive controls for PCR and antigen based tests. Neutralizing monoclonal antibody is available.

5. Research and development of new procedures for diagnosis and control

1. We are evaluating different extraction and enrichment procedures to enhance our ability to detect carrier fish.

2. We are evaluating the potential of controlling CCVD using vaccinations.

3. We have established a BAC system for producing recombinant CCV for vaccine work.

2 Annual reports of OIE Reference Centres, 2011 Channel catfish virus

4. We have established a Microarray that contains 70 CCV genes on it for gene expression studies.

5. We are building and characterizing a CCV case isolate library to be used for evaluating diagnostic tests and genetic/antigenic diversity of CCV.

6. We have performed an initial evaluation of our case library and determined that there are at least 2 genogroups of the virus. We have established a PCR method that distinguishes the genogroups and are in the process of evaluating these tests on a larger number of samples.

6. Collection, analysis and dissemination of epizootiological data relevant to international disease control

 none

7. Maintenance of a system of quality assurance, biosafety and biosecurity relevant to the pathogen and the disease concerned

 none

8. Provision of consultant expertise to OIE or to OIE Member Countries

 none

9. Provision of scientific and technical training to personnel from other OIE Member Countries

 none

10. Provision of diagnostic testing facilities to other OIE Member Countries

 none

11. Organisation of international scientific meetings on behalf of OIE or other international bodies

 none

12. Participation in international scientific collaborative studies

 none

13. Publication and dissemination of information relevant to the work of OIE (including list of scientific publications, internet publishing activities, presentations at international conferences)

 Scientific publications in peer-reviewed journals

 Hanson, L.A. (2012) Ictalurid herpes-like viruses. In C.A. Tidona and G. Darai (eds). The Springer Index of Viruses, 2nd ed., Part 37, 725-733, Springer-Verlag. Berlin, DOI: 10.1007/978-0-387-95919-1_102

 Hanson, L.; A. Dishon; M. Kotler. (2011) Herpesviruses that Infect Fish. Viruses, 3, (11), 2160-2191.

 *Arnizaut, A. B., L. A. Hanson (2011). Antibody response of channel catfish after channel catfish virus infection and following dexamethasone treatment. Diseases of Aquatic Organisms 95: 189-201.

Annual reports of OIE Reference Centres, 2011 3 Channel catfish virus

 Other communications

 Plumb, J.A. and L.A. Hanson. (2011) Health Maintenance and Principal Microbial Diseases of Cultured Fishes, Third Edition. Blackwell Publishing. ISBN 978-0-8138-1693-7

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4 Annual reports of OIE Reference Centres, 2011