Supplementary File

Kinetic, equilibrium and thermodynamic studies of uranium(VI)

biosorption by free and entrapped fresh water micro-algae

Ilkay Acıkgoz Erkaya1, M. Yakup Arica2, Aydın Akbulut3, Gulay Bayramoglu2,4*

1Department of Biology Education, Gazi University, 06500 Teknikokullar, Ankara,

Turkey

2Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500

Teknikokullar, Ankara, Turkey

3Department of Environmental Science, Institute of Natural and Applied Sciences,

Hacettepe University, 06800 Beytepe, Ankara, Turkey

4Department of Chemistry, Faculty of Science, Gazi University, 06500 Teknikokullar,

Ankara, Turkey

*Correspondence: Tel: +90-312-202-1142; E-mail: [email protected] Figure S1. Effect of temperature on the U(VI) biosorption on the free C. reinhardtii

cells, entrapped algae, and bare CMC beads. Figure S2. The Langmuir plots for biosorption of U(VI) on the free C. reinhardtii cells,

entrapped algae, and bare CMC beads. Figure S3. The Pseudo-second kinetic model plots for biosorption of U(VI) on the free

C. reinhardtii cells, entrapped algae, and bare CMC beads. Table S1. Kinetic parameters for biosorption of U(VI) ion by the algae cells, algae immobilized CMC and bare CMC beads from aqueous solutions. Model parameters 1 2 Biosorbent qe,exp qe, cal k1x10 R (mg/g) (mg/g) (min-1) Algae cells 337.2 986.3 1.38 0.896 First-order Immobilized algae 196.8 691.2 1.15 0.914 Bare CMC beads 153.4 58.9 0.56 0.851 1 2 qe,exp qe, cal k2x10 R (mg/g) (mg/g) (g/mg min) Algae cells 337.2 357.1 1.65 0.999 Second- Immobilized algae 196.8 212.8 1.22 0.998 order Bare CMC beads 153.4 166.7 1.04 0.995 -5 2 Ki x10 C R (mg/g min0.5) Algae cells 18.4 172 0.875 Intra- Immobilized algae 12.8 80.9 0.895 particle Bare CMC beads 9.81 67.8 0.823 3 2 α kb x10 R (L/g) Algae cells 0.229 13.2 0.956 Bangham’s Immobilized algae 0.328 2.04 0.961 Bare CMC beads 0.310 1.10 0.932 Table S2. Thermodynamic parameters for biosorption of U(VI) ion by the algae cells, algae immobilized CMC and bare CMC beads from aqueous solutions. ΔG° ΔS° ΔH° Biosorbent (kJ/mol) (J/mol) (kJ/mol K) -25.1 -27.2 Algae cells -28.2 99.7 2.46 -29.3 -21.7 -23.6 Immobilized algae -24.7 94.8 4.57 -25.6 -21.3 -22.8 Bare CMC beads -23.8 85.4 2.55 -24.6