BIO 208 TERMS AND OBJECTIVES s08 Objectives Unit 4 Ch 8,9 + handouts, films

Chapter 7, 8 17 (portions), notes, films, handouts

1. Detail the process involved in the creation of transgenic bacteria and provide an example of a human drug produced by recombinant bacteria 2. Provide the rational and science behind cloning human growth hormone 3. Examine the advantages of cloning genes for human proteins into bacteria 4. Delve into the process of genetically modified plants and their use in agriculture. Provide pros and cons of GM foods. 5. Outline specific GM crops and the advantages (or disadvantages) they provide 6. Examine patent law as it applies to genetically engineered organisms 7. Determine how to regulate tissue specific gene expression so that human proteins are expressed in sheep or goat milk. Examine the process of producing a transgenic goat. 8. Examine a variety of transgenic animals currently available for various uses including xenotransplantation, agriculture, models for human disease 9. Compare and contrast 3 types of cloning: gene, reproductive, and therapeutic 10. View the technique of egg microinjection 11. Review the steps involved in somatic cell nuclear transfer (SCNT) and describe the reason for each step 12. Discuss physiological problems often observed in cloned animals and why human cloning has not been attempted and is illegal 13. Review the steps involved in the generation of embryonic stem cell lines via donated embryos or therapeutic cloning 14. Discuss the ethical aspects of embryonic stem cell research 15. Define the terms: pluripotent, totipotent, and multipotent stem cell, blastocyst, inner cell mass, differentiation 16. Discuss the sources of human stem cells – embryonic and other 17. Formulate an opinion on human therapeutic cloning 18. Review the goals of the Human Genome Project 19. Read an autoradiogram of a DNA sequencing gel 20. Define: Genome, Transcriptome 21. Discuss the field of pharmacogenomics (handout), goals, problems, benefits, drug metabolism differences 22. Explore the use of association studies pharmacogenomics 23. Discuss some of the benefits of pharmacogenomics 24. Describe single nucleotide polymorphisms and their potential usefulness in medicine 25. Relate SNPs to the diagnosis of genetic predispositions and to the cause of some genetic diseases 26. Examine SNPs in melanoma and alcohol dehydrogenase and discuss the association of SNPs with alcohol sensitivity 27. Review the differences people exhibit in drug absorption, metabolism, target receptors, side effects, transport, and excretion. 28. Describe the use of Y chromosome markers (SNPs) in determining ancestry (film, handout) 29. To clone and express the green fluorescent protein gene in bacteria (lab) 30. Compare H. sapiens mitochondrial DNA sequence with Neanderthal and other primate DNA (lab) 2