Amino Acid Availability in the Ovine Fetus in Response to Acute Ethanol Exposure, Acute

Supplemental Table 1

Detailed significances among groups for change in maternal plasma amino acid concentrations at 60 min from baseline (Figure 5)

Saline Alcohol ALC+GLN GLN30 ALC+GLN GLN100 Acidemia (a) (b) 30 (d) 100 (f) (g) (c) (e) GLU b c e f a d g a d g b c e f a d g a d g b c e f PHE e a f e GLN e f c d e f b e f b e f g a b c a b c d e a b c d e CIT b a f b TYR f f a c MET f a LEU b c e f a a a a VAL b e a a ILE e e a c BCAA b c e f a a a a SER b e a f a f b e ASN b e a f e b e a c d f e ASP e b f g e e a = statistically different compared to Saline control group (P < 0.05) b = statistically different compared to Alcohol group (P < 0.05) c = statistically different compared to ALC+GLN30 (alcohol + 30 mg glutamine/kg BW) group (P < 0.05) d = statistically different compared to GLN30 (30 mg glutamine/kg BW) group (P < 0.05) e = statistically different compared to ALC+GLN100 (alcohol + 100 mg glutamine/kg BW) group (P < 0.05) f = statistically different compared to GLN100 (100 mg glutamine/kg BW) group (P < 0.05) g = statistically different compared to Acidemia group (P < 0.05)

Supplemental Table 2

1 Detailed significances among groups for change in fetal plasma amino acid concentrations at 60 min from baseline (Figure 6)

Saline Alcohol ALC+GLN GLN30 ALC+GLN GLN100 Acidemia (a) (b) 30 (d) 100 (f) (g) (c) (e) ARG d b e f d d PHE b a GLN b f a c d e f g b f b f b a b c d b CIT g g g b e f VAL f a g f ALA f g f g b e b e ASN b a g g b e ASP f b TRP b a d f g B b b

a = statistically different compared to Saline control group (P < 0.05) b = statistically different compared to Alcohol group (P < 0.05) c = statistically different compared to ALC+GLN30 (alcohol + 30 mg glutamine/kg BW) group (P < 0.05) d = statistically different compared to GLN30 (30 mg glutamine/kg BW) group (P < 0.05) e = statistically different compared to ALC+GLN100 (alcohol + 100 mg glutamine/kg BW) group (P < 0.05) f = statistically different compared to GLN100 (100 mg glutamine/kg BW) group (P < 0.05) g = statistically different compared to Acidemia group (P < 0.05)