The Use Of Sigma Molecular Weight Markers For Gel Filtration Chromatography.

Molecular weight markers for Gel filtration chromatography.Sigma product MW-GF-200.Contains 10mg cytochrome c, 15mg carbonic anhydrase, 50mg bovine serum albumen containing 0.3% dithiothreitol, 25mg alcohol dehydrogenase,15mg beta amylase containing 15% sodium chloride, 4%glucose and 1% dithiothreitol , 50mg blue dextran. 165mg material per kit. Harmful R42-May cause sensitation by inhalation. Sigma recommend avoiding contact and inhalation.The full toxicological properties have not been fully investigated. 25 or 50mM Tris buffer containing 0.1 or 1.0mM EDTA 500mls N.S.H. FPLC Purified inorganic pyrophosphatase enzyme samples. 0.5mls NO RISK PHRASES AVAILABLE. Avoid inhalation,skin and eye contact. May be possible biological hazard see assessment 1683/G33. Hazardous Substances Policy - Assessment CHEMICAL HAZARD AND RISK ASSESSMENT School of Biosciences

Name of Dr. T. W. Young Assessment Number* 1798 supervisor Signature Assessor C.Sampayo Date of Assessment 03-05-2001 Signature

Notes A School COSHH form in Word is available on the School Server. Available from the Health and Safety Unit. Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment. Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form. Use a continuation sheet to expand any section of this form in hard copy version.

1 LOCATION OF THE WORK ACTIVITY Laboratory G6

2 PERSONS WHO MAY BE AT RISK List names where possible Dr. T. W. Young, A. Wadeson, Ph.D. students

3 ACTIVITY ASSESSED The use of Sigma molecular weight markers for Gel filtration chromatography.

4 MATERIALS INVOLVED Attach copies of data sheet(s) NAME AMOUNT HAZARD RISK PHRASES HAZDAT NO*** BIOSCIENCESNO*** max/stored Molecular weight 165mg Harmful R42-May cause sensitation markers for Gel filtration material by inhalation. Sigma chromatography.Sigma per kit. recommend avoiding product MW-GF- contact and inhalation.The 200.Contains 10mg full toxicological properties cytochrome c, 15mg have not been fully carbonic anhydrase, investigated. 50mg bovine serum albumen containing 0.3% dithiothreitol, 25mg alcohol dehydrogenase,15mg beta amylase containing 15% sodium chloride, 4%glucose and 1% dithiothreitol , 50mg blue dextran. 25 or 50mM Tris buffer 500mls N.S.H. containing 0.1 or 1.0mM EDTA FPLC Purified inorganic 0.5mls NO RISK PHRASES pyrophosphatase enzyme AVAILABLE. Avoid samples. inhalation,skin and eye contact. May be possible biological hazard see assessment 1683/G33.

5 INTENDED USE** Give brief details and attach protocol/instructions To be used as markers for molecular Weight identification for gel filtration chromatography.

6 RISKS to HEALTH and SAFETY from INTENDED USE From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk? From the stock molecular weight reagents there is a risk of skin and eye contact and risk of inhalation of dust and vapours which may also cause sensitisation especially by inhalation in the case of beta amylase. There is a risk of skin and eye contact and inhalation of Aerosols from all the solutions when handling and pipetting. The possible Biological risk from the FPLC purified inorganic pyrophosphatase enzyme solution is small but contact Should be avoided .

7 CONCLUSIONS ABOUT RISKS Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary? The risks associated with all the compounds and reagents are significant. Control measures should be taken As detailed in section 8,together with Good Chemical Practice.

8 CONTROL MEASURES Additional to Good Chemical Practice In addition to Good Chemical Practice, care should be taken to minimise inhalation of substances and aerosols especially where they contain potentially sensitising substances. Appropriate face masks should be used . Gloves and goggles should be worn. Waste containing molecular weight Markers, this includes such items as pipette tips, and contaminated equipment and glassware should be rinsed and bagged prior to disposal or transffering to the normal wash up. Check integrity of gloves and face mask and eye glasses before using.

9 INSTRUCTION/TRAINING Specify course(s) and/or special arrangements. General Laboratory Practice Only experienced laboratory personnel should be using these compounds and reagents.

10 MONITORING Performance of control measures,

Personal exposure Health Surveillance

11 WASTE DISPOSAL PROCEDURE See School Server for Approved Procedure Document on specific Chemical Waste Disposal. Small quantities of solutions containing tris buffer is disposed of to the drain. Chemicals can be disposed of according to the hazardous substances policy of the University.

12 REVIEW Enter the date or circumstances for review of assessment (maximum review interval 5 years) April 2006. 0r any change in protocol or amounts used. 13 EMERGENCY ACTION TO CONTROL HAZARDS To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc. Only adequately trained and experienced personnel should deal with emergency spills. Solutions involved are small volumes .A chemical spill kit can be used.

TO PROTECT PERSONNEL Evacuation, protection for personnel involved in clean-up, Special First Aid Only adequately trained and experienced personnel should deal with emergency spills. Gloves and eye glasses and appropriate face mask should be worn to prevent contact with spills of the above Solids and solutions.

TO RENDER SITE OF EMERGENCY SAFE Clean-up/decontamination Wash spill site with soap and water and dry.

DR YOUNG/A.WADESON. 3523 CONTACT PHONE

10.10.00 * Prefix T is used for Teaching Assessment Number.

** Please include amount of chemicals used and how.

*** Hazdat No is the UNICOSHH datasheet report number.

Biosciences No is the Biosciences data sheet number.

UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER. BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.